ORIGINAL RESEARCH article
Front. Pediatr.
Sec. Pediatric Urology
Volume 13 - 2025 | doi: 10.3389/fped.2025.1628435
Acellular Foreskin Dermal Matrix is Efficient in Supporting the Growth of urothelial cells Derived from Hypospadias Patients
Provisionally accepted
- 1Jiangmen Maternity and Child Health Care Hospital, Jiangmen, China
- 2Shanghai Eighth People’s Hospital, shanghai, China
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Hypospadias is a common congenital defect in males, with surgery remaining the primary treatment option. However, urethral reconstruction procedures often require additional tissue transplantation, which is limited by the availability of suitable tissue sources. In this study, we prepared acellular dermal matrix (ADM) from foreskin obtained through circumcision and isolated urothelium-derived cells from patients with hypospadias. We then evaluated the growth of these urothelium-derived cells on the ADM. Our results confirmed successful decellularization of the foreskin dermal tissues and demonstrated that the resulting ADM exhibited minimal cytotoxicity toward primary urothelium-derived cells. CFSE and CCK-8 staining assays revealed robust urothelial cell growth on the ADM. Furthermore, the ADM with growing urotheliumderived cells displayed superior biomechanical properties compared to native ADM, suggesting that foreskin ADM is an excellent scaffold for urothelial cell growth. Animal studies further confirmed the ADM's excellent biocompatibility, showing no significant inflammatory response and promoting vascularization while maintaining its critical hairless property. These findings indicate that foreskin ADM is a promising alternative material for tissue engineering in the treatment of conditions like hypospadias that require urethral reconstruction.
Keywords: Hypospadias, acellular dermal matrix (ADM), urothelium-derived cells, alternative material for tissue engineering, Urethral reconstruction
Received: 14 May 2025; Accepted: 21 Jul 2025.
Copyright: © 2025 Cao, Yang, Ye, Xiong and Zhu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Zhiqing Cao, Jiangmen Maternity and Child Health Care Hospital, Jiangmen, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.