ORIGINAL RESEARCH article
Front. Pediatr.
Sec. Pediatric Infectious Diseases
This article is part of the Research TopicNew Discoveries and Challenges in Pediatric Infectious Diseases: Epidemiological, Clinical, and Pathogenic AdvancesView all 13 articles
Rapid diagnostic value of next-generation sequencing-based technologies in childhood pneumonia
Provisionally accepted
- 1Yangzhou Maternity and Child Health Care Hospital, Yangzhou, China
- 2The Affiliated Taizhou People's Hospital of Nanjing Medical University, Taizhou, China
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Background: This study evaluates the diagnostic efficacy of next-generation sequencing (NGS) in pediatric patients with suspected pneumonia and unidentified etiologies. Objective: This retrospective study encompassed pediatric patients with suspected pneumonia, spanning the period from January 2022 to December 2023. Nasal swabs and blood samples were collected for a comprehensive diagnostic panel, including NGS, blood culture, complete blood count, and serum biomarkers. Methods: Routine diagnostic tests were compared with NGS for turnaround time and diagnostic accuracy. Patients were categorized based on clinical diagnosis into non-pneumonia and pneumonia groups. Logistic regression analysis was performed to identify independent predictors of pneumonia. Results: NGS provided results within 24 hours, significantly faster than conventional bacterial cultures (3-5 days). The positivity rate for pathogen identification increased from 55.3% with traditional methods to 86.2% with NGS (p<0.05). Serum levels of procalcitonin, creatinine, and C-reactive protein were elevated in pneumonia patients, while albumin levels were decreased. Logistic regression identified C-reactive protein and albumin as independent predictors of pneumonia. The area under the receiver operating characteristic curve for NGS was superior to conventional methods and serum biomarkers alone or in combination. Conclusion: NGS is a promising tool for rapid and accurate etiologic diagnosis of pneumonia in children. The combination of NGS with albumin levels may serve as an effective screening strategy, potentially enhancing clinical management through earlier intervention and targeted therapy. Further validation in larger cohorts is warranted to establish the clinical utility of this approach.
Keywords: Pneumonia, Pneumococcus pneumoniae, Polymerase Chain Reaction, Blood, diagnosis
Received: 09 Jul 2025; Accepted: 24 Nov 2025.
Copyright: © 2025 Chen, Zhu, Fang, Ma, Zhang and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Hongxia Liu
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