Missing information
Add information about chemical formula, Table 2, polarization and antibody list.
TABLE 2
| Antibodies | Cat No. | Manufacturer | Sources of species |
|---|---|---|---|
| Fibronectin | ab2413 | abcam | Rabbit |
| Collagen-Ⅰ | 66761-1-Ig | proteintech | Mouse |
| IκBα | #4814 | cell signaling technology | Mouse |
| p38 | #8690 | cell signaling technology | Rabbit |
| P-p38 | #4511 | cell signaling technology | Rabbit |
| Arginase-1 | ab233548 | abcam | Rabbit |
| ASK-1 | 67072-1-Ig | proteintech | Rabbit |
| P-ASK-1 | 28846-1-AP | proteintech | Rabbit |
| NF-kB | ab32536 | abcam | Rabbit |
| P-NF-kB | ab76302 | abcam | Rabbit |
| iNOS | 18985-1-AP | proteintech | Rabbit |
| β-Actin | ab8226 | abcam | Mouse |
(Antibody list) Primary antibodies for Western Blot.
Chemical formula
Chemical structure of AKEX0011
Molecular formula C18H14N5O2F3, mass 389.3 g/mol, CAS:1590403-33-0.
Polarization
RAW 264.7 cells are a macrophage-like, Abelson leukemia virus-transformed cell line derived from BALB/c mice. This macrophage cell line is commonly used to study phagocytosis, apoptosis, inflammation, as well as M1/M2 polarization. Polarization of M1/M2 phenotype can be determined by the expression of specific M1 (CD80, CD86) and M2 (CD206, CD163) markers detected by flow cytometry. In the in vitro experiments of our study, we detected the expression of CD86 and CD163 in RAW264.7 cells of each group by flow cytometry to explore macrophage polarization. Our flow cytometry results confirmed that silica induced polarization of RAW264.7 from M0 macrophages toward certain M1 subtype (F4/80 + CD86+), but have no effect on the polarization towards M2 subtype (F4/80 + CD163+).
Statements
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Summary
Keywords
AKEX0011, macrophage polarization, pirfenidone, pulmonary fibrosis, silicosis
Citation
Fan M, Xiao H, Song D, Zhu L, Zhang J, Zhang X, Wang J, Dai H and Wang C (2023) Addendum: A novel N-Arylpyridone compound alleviates the inflammatory and fibrotic reaction of silicosis by inhibiting the ASK1-p38 pathway and regulating macrophage polarization. Front. Pharmacol. 13:1108989. doi: 10.3389/fphar.2022.1108989
Received
27 November 2022
Accepted
29 November 2022
Published
18 January 2023
Volume
13 - 2022
Edited and reviewed by
Paola Patrignani, University of Studies G. d'Annunzio Chieti and Pescara, Italy
Updates
Copyright
© 2023 Fan, Xiao, Song, Zhu, Zhang, Zhang, Wang, Dai and Wang.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Jing Wang, wangjing@ibms.pumc.edu.cn; Huaping Dai, daihuaping@ccmu.edu.cn; Chen Wang, wangchen@pumc.edu.cn
This article was submitted to Inflammation Pharmacology, a section of the journal Frontiers in Pharmacology
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.