CORRECTION article

Front. Pharmacol., 31 October 2023

Sec. Gastrointestinal and Hepatic Pharmacology

Volume 14 - 2023 | https://doi.org/10.3389/fphar.2023.1256600

Corrigendum: Effects of Xiao Chengqi formula on slow transit constipation by assessing gut microbiota and metabolomics analysis in vitro and in vivo

  • 1. Department of Coloproctology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China

  • 2. Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China

  • 3. Guangdong Institute of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China

In the published article, there was an error in Figure 10 as published. In Figure 10C, the PD+L+B apoptosis figure was misplaced with ST+L+B figure while drawing. The corrected Figure 10 and its caption appear below.

FIGURE 10

Additionally, the “TSN” and “AGS and HGC-27 cells” words showed in the Materials and Methods section were incorrect.

A correction has been made to Materials and Methods, Cell Viability, Paragraph 1. This sentence previously stated:

“The effect of TSN on cell viability was tested using the Cell Counting Kit-8 (CCK-8) assay (Nanjing KeyGen Biotech Co., Ltd., Nanjing, Jiangsu, China). Briefly, cells were seeded into 96- well plates (3 × 103 cells/well) and treated with TSN (0.5–2 µM).”

The corrected sentence appears below:

“The effect of XCQ formula on cell viability was tested using the Cell Counting Kit-8 (CCK-8) assay (Nanjing KeyGen Biotech Co., Ltd., Nanjing, Jiangsu, China). Briefly, cells were seeded into 96-well plates (5 × 103 cells/well) and treated with Butamben or Loperamide.”

A correction has been made to Materials and Methods, Cell Cycle Analysis, Paragraph 1. This sentence previously stated:

“AGS and HGC-27 cells were incubated with TSN for the indicated times, after which the cells were collected, washed, and fixed with 66% cold ethanol at 4°C overnight.”

The corrected sentence appears below:

“ICC cells were incubated with Butamben or Loperamide for the indicated times, after which the cells were collected, washed, and fixed with 66% cold ethanol at 4°C overnight.”

A correction has been made to Materials and Methods, Cell Apoptosis Analysis, Paragraph 1. This sentence previously stated:

“Cells were treated with or without TSN for the indicated times, after which the cells were collected, washed, and suspended in 500 µL of binding buffer, and mixed with 5 µL of annexin V-FITC and 5 µL of propidium iodide.”

The corrected sentence appears below:

“Cells were treated with or without specific drugs for the indicated times, after which the cells were collected, washed, and suspended in 500 µL of binding buffer, and mixed with 5 µL of annexin V-FITC and 5 µL of propidium iodide.”

Lastly, the figure insertion was mislabeled in Results, RNA-Sequencing section.

A correction has been made to the Results section, subsection RNA-Sequencing, Paragraph 1. This sentence previously stated:

“Furthermore, we validated the changes in CD117, IL-21R, and their downstream molecules at the protein level (Figures 5B–E).”

The corrected sentence appears below:

“Furthermore, we validated the changes in CD117, IL-21R, and their downstream molecules at the protein level (Figures 5B–F).”

The authors apologize for these errors and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

Statements

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Summary

Keywords

Xiao Chengqi formula, traditional Chinese medicine, slow transit constipation, butyl aminobenzene, interstitial cells of cajal, interleukin-21 receptor

Citation

Zhou Q, Zhang D, Zhang H, Wan X, Hu B, Zou Q, Su D, Peng H, Huang D and Ren D (2023) Corrigendum: Effects of Xiao Chengqi formula on slow transit constipation by assessing gut microbiota and metabolomics analysis in vitro and in vivo. Front. Pharmacol. 14:1256600. doi: 10.3389/fphar.2023.1256600

Received

11 July 2023

Accepted

12 October 2023

Published

31 October 2023

Volume

14 - 2023

Edited and reviewed by

Angelo A. Izzo, University of Naples Federico II, Italy

Updates

Copyright

*Correspondence: Donglin Ren, ; Dandan Huang,

† These authors have contributed equally to this work and share first authorship

Disclaimer

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

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