ORIGINAL RESEARCH article
Front. Pharmacol.
Sec. Cardiovascular and Smooth Muscle Pharmacology
Volume 16 - 2025 | doi: 10.3389/fphar.2025.1578913
This article is part of the Research TopicInnovative Approaches and Molecular Mechanisms in Cardiovascular PharmacologyView all 14 articles
Bitter Taste Receptor Agonists Induce Vasorelaxation in Porcine Coronary Arteries
Provisionally accepted
- 1E-Da Hospital, Kaohsiung City, Taiwan
- 2I-Shou University, Kaohsiung, Taiwan
- 3Ikutora Clinic, Hokkaido, Japan
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Background: Ischemic heart disease (IHD) remains a leading cause of global morbidity and mortality, necessitating the search for novel therapeutic approaches. Recent studies have identified bitter taste receptors (TAS2Rs) in vascular smooth muscle cells as potential therapeutic targets because of their vasorelaxant properties. This study investigated the vasorelaxant effects of TAS2R agonists on porcine coronary arteries ex vivo and explored their potential as novel therapeutic targets for IHD.Methods: Isolated porcine coronary artery rings were precontracted using U46619 and treated with TAS2R agonists, including flufenamic acid, dapsone, phenanthroline, chloroquine, and quinine. Vasorelaxation induced by TAS2R agonists was quantitatively assessed, and pharmacological inhibitors were used to elucidate the underlying mechanisms of vasorelaxation. Real-time PCR analysis was conducted to confirm the expression of specific TAS2R subtypes in porcine coronary arterial tissue.Results: TAS2R agonists induced concentration-dependent vasorelaxation, with flufenamic acid showing potent effects, exhibiting an EC50 of 30.4 μM, whereas phenanthroline and chloroquine exhibited moderate responses. In contrast, quinine and dapsone showed mild relaxation. The flufenamic acid-induced effect was attenuated by NG-nitro-L-arginine (47.4 ± 3.04%), apamin (49.2 ± 3.7%), and glibenclamide (49.6 ± 1.5%), indicating the involvement of nitric oxide signaling and potassium channels. PCR analysis revealed the differential expression of TAS2R subtypes, with TAS2R42 showing the highest expression, followed by subtypes 40, 10, and 38.This study showed that TAS2R agonists, especially flufenamic acid, phenanthroline, and chloroquine, induced vasorelaxation in isolated porcine coronary arteries. The vasorelaxation mechanism of flufenamic acid may involve nitric oxide signaling and potassium channels. The expression of specific TAS2R subtypes, together with functional observations, suggest that bitter taste receptors play a role in coronary vascular regulation, warranting further investigation into their therapeutic potential.
Keywords: TAS2R agonists, Vasorelaxation, Ischemic Heart Disease, bitter taste receptors, Porcine coronary arteries, Nitric Oxide Signaling, Potassium Channels
Received: 18 Feb 2025; Accepted: 25 Jun 2025.
Copyright: © 2025 Tsai, Li, Chang and Huang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Shih-Che Huang, Ikutora Clinic, Hokkaido, Japan
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.