ORIGINAL RESEARCH article
Front. Pharmacol.
Sec. Ethnopharmacology
Volume 16 - 2025 | doi: 10.3389/fphar.2025.1612233
Nymphaea 'Eldorado' Flower Extract Targets Serpine 1 to Attenuate Inflammatory and Antioxidant Crosstalk in Zebrafish
Provisionally accepted
Liyan Mao1
Ling Yun Long1Qian Qin1Qiuwei Huang1Qiulan Huang1Yanping Yu1Liqiong Ding1Gongde Liu1Ji Zhang1
Beimi Cui2
Xiaohui Tan1*- 1Guangxi Academy of Agricultural Science, Nanning, China
- 2Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom
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Chronic inflammation and oxidative stress are pivotal drivers of pathological conditions, necessitating safer plant-derived therapeutic alternatives. This study elucidates the anti-inflammatory and antioxidant mechanisms of Nymphaea 'Eldorado' flower water extract (NEWE) in a zebrafish (Danio rerio) model of copper sulfate (CuSO₄)-induced inflammation. NEWE (25-100 µ g/mL) attenuates CuSO₄-triggered neutrophil migration and oxidative stress by downregulating proinflammatory genes (il1b, ptgs2a/b) and reducing reactive oxygen species (ROS) production. Transcriptomic profiling identified 339 differentially expressed genes (DEGs), enriched in cytokine signaling and redox regulation, with serpine1, stat3, and mmp9 emerging as key regulatory hubs. Widely targeted metabolomics revealed 891 bioactive compounds, including flavonoids and phenylpropanoids, with network pharmacology predicting multi-target interactions involving inflammatory and oxidative stress pathways. Molecular docking confirmed binding affinities between protocatechuic acid, L-pyroglutamic acid, and Serpine1's active site, indicating direct interference with inflammation modulation. Collectively, these results establish NEWE as a polypharmacological agent that disrupts inflammation-oxidative stress crosstalk primarily through Serpine1-mediated pathways, offering a molecular foundation for plant-derived interventions against chronic inflammatory diseases.
Keywords: Nymphaea 'Eldorado', anti-inflammatory, antioxidant, Zebrafish, Transcriptomics, molecular docking
Received: 17 Apr 2025; Accepted: 23 Jun 2025.
Copyright: © 2025 Mao, Long, Qin, Huang, Huang, Yu, Ding, Liu, Zhang, Cui and Tan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Xiaohui Tan, Guangxi Academy of Agricultural Science, Nanning, China
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