ORIGINAL RESEARCH article
Front. Pharmacol.
Sec. Ethnopharmacology
Antioxidant, antiaging and mitochondrial protective effects of JadeAging in Caenorhabditis elegans
Provisionally accepted
- 1Shandong University of Traditional Chinese Medicine, Jinan, China
- 2Qingdao Chenland Pharmaceutical Co., Ltd, Qingdao, China
- 3Shandong University of Traditional Chinese Medicine Affiliated Hospital, Jinan, China
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Objective: This study aims to evaluate the antioxidant, anti-aging, and mitochondrial protective effects of JadeAging, a Chinese medicine-based longevity formulation, using the model organism Caenorhabditis elegans. Methods: We used C. elegans to assess the impact of JadeAging and its individual components (rehmannia, poria, and ginseng) on various health metrics. RNA sequencing (RNA-seq) was performed to identify differentially expressed genes (DEGs) and their effects on biological pathways. Lifespan and healthspan assays were performed to evaluate cell vitality, whereas mitochondrial health was assessed using mitochondrial fragmentation analysis. Antioxidant capacity was determined by measuring protection against reactive oxygen species (ROS). Results: Treatment with JadeAging did not significantly extend lifespan but showed improvements in healthspan, with treated animals exhibiting more vigorous movement over their lifespan. JadeAging and its components demonstrated significant antioxidant capacity, protecting C. elegans from the deleterious effects of paraquat-induced oxidative stress. Furthermore, JadeAging treatment significantly reduced mitochondrial fragmentation, indicating enhanced mitochondrial health. RNA-Seq data revealed downregulation of daf-2 and rsks-1 and upregulation of cpr-1, suggesting the activation of the TOR pathway and promotion of autophagy. Conclusion: JadeAging exerts protective effects against oxidative stress and age-related mitochondrial decline, likely by modulating key longevity pathways. These findings support the positive effects of JadeAging and its components on health.
Keywords: JadeAging, antioxidant, anti-aging, mitochondrial health, Caenorhabditis elegans, TOR pathway
Received: 19 Jun 2025; Accepted: 26 Nov 2025.
Copyright: © 2025 Lu, Yin, Wang, Wang, Zou and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Bowen Lu
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