ORIGINAL RESEARCH article
Front. Pharmacol.
Sec. Experimental Pharmacology and Drug Discovery
Anwuligan promote the Transition of Hair Follicle Cycle via Wnt/β-catenin signaling pathway
Provisionally accepted
- Changzhi Medical College, Changzhi, China
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Hair loss is a significant dermatological condition and hair regeneration depends on orderly cycle of hair follicle activity. Traditional Chinese Medicine (TCM) suggests that BaoHeWan can improve dry and sparse hair, but the molecular mechanisms behind this effect are not well known. This study utilizes network pharmacology, transcriptomics and molecular biology methods to investigate how BaoHeWan promote hair regeneration. Using network pharmacology analysis and molecular docking, anwuligan was found to be a major active ingredient in BaoHeWan, potentially acting by binding to CTNNB1. Phenotype observation and H&E staining demonstrated that anwuligan promoted hair regeneration in model mice. Transcriptomics analysis of mice treated with anwuligan, complemented by cellular validation, demonstrated that anwuligan induces upregulation of Ctnnb1 expression, activates the Wnt/β-catenin signaling pathway, promotes the proliferation of hair follicle stem cells (HFSCs), and facilitates the progression of the hair follicle cycle. These results elucidate the molecular mechanisms underlying anwuligan's facilitation of hair regeneration and offer a scientific foundation for the utilization of TCM in the treatment of hair loss.
Keywords: anwuligan, cycle transition, Hair Follicle, Hair regeneration, Traditional Chinese Medicine, Wnt/β-catenin
Received: 12 Sep 2025; Accepted: 18 Dec 2025.
Copyright: © 2025 Li, Zhang, Li and Zheng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Fang Li
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