ORIGINAL RESEARCH article
Front. Pharmacol.
Sec. Pharmacology of Anti-Cancer Drugs
This article is part of the Research TopicUnlocking Autophagy’s Full Potential: Embracing a Multidimensional Approach for Targeted Cancer TreatmentView all 9 articles
Targeting the AKT/mTOR Axis: Pectolinarigenin Induces Autophagy and Apoptosis in Human Cervical Cancer Cells
Provisionally accepted
Yaoyao Fang1
Jing Bai2Sijia Bo1Xiaoli Cui3Haiping Song4Li Guo5Zhaohui Luan4Qixuan Sui4Yingchun Zheng4
Li Sun4*- 1School of Clinical Medicine, Shandong Second Medical University, Weifang, Shandong Province, China
- 2Jinan Maternity And Child Care Hospital, Jinan, Shandong Province, China
- 3Pingdu People's Hospital, Qingdao, Shandong Province, China
- 4First Department of Gynecology Oncology, Qingdao Central Hospital, University of Health and Rehabilitation Sciences (Qingdao Central Hospital), Qingdao, China., Qingdao, China
- 5Qingdao Hospital, University of Health and Rehabilitation Sciences (Qingdao Municipal Hospital), Qingdao, China
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Cervical cancer (CC) remains a significant global health issue, accounting for approximately 7% of all cancer cases in women. This study investigated the anti-cancer potential of pectolinarigenin (PEC), a bioactive compound derived from plants, aiming to explore its therapeutic effects and underlying mechanisms against CC. By integrating network pharmacology analysis with cellular assays, we identified 13 key targets of PEC related to CC, with molecular docking highlighting AKT as a primary target. Experimentally, PEC demonstrated strong anti-cancer effects on cervical cancer both in vivo and in vitro. Western blotting analysis revealed that PEC treatment led to a dose-dependent decrease in Bcl-2 protein levels, coupled with increased activation of pro-apoptotic markers Bax and cleaved caspase-3 in both cell lines. PEC also elevated the levels of LC3B II protein, indicating the induction of autophagy. Notably, this autophagic response was inhibited by 3-MA, an autophagy inhibitor, suggesting that PEC played a regulatory role in activating autophagy. Mechanistic studies confirmed that PEC effectively suppressed the AKT/mTOR signaling pathway, a critical regulator of both autophagy and apoptosis in cancer cells. Overall, this is the first study to demonstrate that PEC exerted potent anti-cancer effects against CC by concurrently inducing autophagy and apoptosis through targeted inhibition of the AKT/mTOR pathway. These findings highlighted the potential of PEC as a promising natural therapeutic agent for CC, paving the way for new treatment strategies. Further comprehensive research is warranted to fully explore PEC's therapeutic capabilities and to develop innovative anti-cancer therapies.
Keywords: Akt/mTOR signaling pathway, apoptosis and autophagy, cervical cancer, Network Pharmacology, Pectolinarigenin
Received: 06 Mar 2025; Accepted: 16 Feb 2026.
Copyright: © 2026 Fang, Bai, Bo, Cui, Song, Guo, Luan, Sui, Zheng and Sun. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Li Sun
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