ORIGINAL RESEARCH article
Front. Vet. Sci.
Sec. Veterinary Epidemiology and Economics
Volume 12 - 2025 | doi: 10.3389/fvets.2025.1619301
Epidemiological survey and Genotype diversity of Mycoplasma hyopneumoniae in Guangxi China swine herds based on MLST
Provisionally accepted
Ying Zhou
Shuo Zhao
Longhua LiangYibin Qin
Cheng KeZhongwei ChenBinxia LuQuanpeng DuanYing PengYu HuangZhenhua DuanKaiyi JiangXunye YangYing He*- Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, China
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Mycoplasma hyopneumoniae (Mhp) infection is a significant challenge for pig farms across Guangxi, yet a comprehensive understanding of its prevalence and molecular characteristics remains elusive. This study sought to define the circulating Mhp genotypes and their distribution in the region's swine population. From 2022 to 2023, 1362 pig lung samples were collected randomly from 14 regions across Guangxi. Mhp detection was performed utilizing a TaqMan Real-time PCR assay. Samples exhibiting strong positive results were selected for multilocus sequence typing (MLST) to assess genetic relationships. Of the 1362 samples collected, 655 tested positive for Mhp. Successful MLST amplification of three housekeeping genes (adk, rpoB, and tpiA) was achieved for 61 of these positive samples. Twenty-seven sequence types, including 24 novel sequences, were identified across the 14 sampled regions in Guangxi Province. Phylogenetic analysis demonstrated a predominance of Mhp types II and V circulating in Guangxi. Overall, a high Mhp incidence rate coupled with considerable genetic diversity was observed. This study represents one of the most comprehensive analyses of Mhp incidence and molecular typing in Guangxi to date. The findings expand the existing knowledge base of Mhp epidemiology in China and contribute a theoretical foundation for developing preventative and control strategies against Mhp in Guangxi.
Keywords: Mycoplasma hyopneumoniae, genotyping, mlst, Swine epidemiology, Guangxi
Received: 07 May 2025; Accepted: 09 Jul 2025.
Copyright: © 2025 Zhou, Zhao, Liang, Qin, Ke, Chen, Lu, Duan, Peng, Huang, Duan, Jiang, Yang and He. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Ying He, Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.