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Front. Chem. | doi: 10.3389/fchem.2018.00197

Ultrasound-assisted esterification of valeric acid to ethyl valerate promoted by biosilicified lipases

Soledad Cebrian-Garcia1,  Alina M. Balu1 and  Rafael Luque1*
  • 1Quimica Organica, Universidad de Córdoba, Spain

A novel, environmentally friendly and sustainable ultrasound-assisted methodology in the valorisation of valeric acid to alkyl valerate using a biosilicified Lipase from Candida Antarctica (LCA) is reported. This one-pot room temperature methodology of enzyme biosilicification leads to biosilicified lipases (Bio-LCA) with improved activity and reaction efficiency as compared to free enzymes. Yields in the ultrasound-promoted esterification of valeric acid was ca. 90% in two hours with 15% m/v of Bio-LCA (616 U/ mg biocatalyst enzymatic activity) and a molar ratio 1:2 (valeric acid: ethanol), slightly superior to that observed by the free enzyme (75% conversion, 583U/mg biocatalyst enzymatic activity). The reuse of enzymes in these conditions was tested and the results show a relatively good reusability of these biosilicified enzymes under the investigated conditions, particularly preserving fairly stable specific activities (616 vs 430 U/mg biocatalyst after four reuses).

Keywords: Biosilicification, Esterification, Ultrasounds, Biocatalysis, Lipases

Received: 02 Feb 2018; Accepted: 15 May 2018.

Edited by:

Florent Allais, AgroParisTech Institut des Sciences et Industries du Vivant et de L'environnement, France

Reviewed by:

Victor Sans Sangorrin, University of Nottingham, United Kingdom
Alexei Lapkin, University of Cambridge, United Kingdom  

Copyright: © 2018 Cebrian-Garcia, Balu and Luque. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Rafael Luque, Universidad de Córdoba, Quimica Organica, Córdoba, Spain, q62alsor@uco.es