Original Research ARTICLE
Toward multi-parametric porous silicon transducers based on covalent grafting of graphene oxide for biosensing applications
- 1Istituto per la Microelettronica e Microsistemi, Consiglio Nazionale delle Ricerche, Italy
- 2Università degli Studi di Napoli Federico II, Italy
Graphene oxide (GO) is a two-dimensional material with peculiar photoluminescence emission and good dispersion in water, that make it an useful platform for the development of label-free optical biosensors. In this study, a GO-porous silicon (PSi) hybrid device is realized using a covalent chemical approach in order to obtain a stable support for biosensing applications. Protein A, used as bioprobe for biosensing purposes, is covalently linked to the GO, using the functional groups on its surface, by carbodiimide chemistry. Protein A bioconjugation to GO-PSi hybrid device is investigated by atomic force microscopy (AFM), scanning electron microscopy (SEM), water contact angle (WCA) measurements, Fourier transform infrared (FTIR) spectroscopy, steady-state photoluminescence (PL) and fluorescence confocal microscopy. PSi reflectance and GO photoluminescence changes can thus be simultaneously exploited for monitoring biomolecule interactions as in a multi-parametric hybrid biosensing device.
Keywords: porous silicon, graphene oxide, Covalent grafting on Si, Photoluminescence, Optical device
Received: 10 Sep 2018;
Accepted: 08 Nov 2018.
Edited by:Thierry Djenizian, École des Mines de Saint-Étienne - Campus Georges Charpak Provence, France
Reviewed by:Rabah Boukherroub, UMR8520 Institut d'électronique, de microélectronique et de nanotechnologie (IEMN), France
Ciro Chiappini, King's College London, United Kingdom
Copyright: © 2018 Moretta, Terracciano, Dardano, Casalino, De Stefano, Schiattarella and Rea. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Dr. Luca De Stefano, Istituto per la Microelettronica e Microsistemi, Consiglio Nazionale delle Ricerche, Catania, 95121, Sicily, Italy, firstname.lastname@example.org