Biomedical Applications of Metallosupramolecular Assemblies—Structural Aspects of the Anticancer Activity

Introduction

The coordination bond directed self-assembly processes have been broadly exploited in building metallosupramolecular species with diverse composition (Bilbeisi et al., 2014), discrete size and shape (Schmidt et al., 2014; Cook and Stang, 2015), and variety of useful properties. Unarguably, the aesthetics of these highly symmetric metallosupramolecules has been the initial source of inspiration for many chemists. This resulted in not only building a myriad of fascinating structures but also in establishing the main principles in the design and synthesis of metallosupramolecular complexes with predefined shapes by exploiting the bond directionality and the coordination properties of the metal ions, and intelligent design of the linking organic ligands (Datta et al., 2018). Nowadays, the main targets are the specific properties of the metallosupramolecular complexes in view of their practical applications that range from chemical catalysis to multi-purpose and stimuli-responsive advanced materials (McConnell et al., 2015). The demonstrated high potential to purposefully combine the properties of metal ions with that of organic ligands has recently attracted the attention of bioinorganic chemists which added up more facets to the range of practical use of the metallosupramolecules—their biological application for therapy and/or diagnostics (Casini et al., 2017; Leung et al., 2017).

The continuous studies related to the clinical use of Pt-complexes as anticancer drugs have evolved to establishing structure-activity relationships for this class of compounds. It is, therefore, not surprising that the first studies on the potential biological applications of metallosupramolecules had focused on anticancer activity of Pt-linked metallosupramolecular cycles (Mounir et al., 2007) and interaction of Fe- or Ru-linked metallohelicates with DNA fragments (Hotze et al., 2006; Malina et al., 2008). This had also given impetus to explore a broader range of metal complexes including the organometallic half-sandwich structures (Aird et al., 2002). Some of the initial examples for studying in details the anticancer activity profiles of coordination driven self-assemblies are the organometallic Ru(II) metallacycles and cages that have been extensively investigated (Therrien et al., 2008; Smith and Therrien, 2011; Dubey et al., 2013; Vajpayee et al., 2013). Thereby, the topic of metal-based drugs had naturally reached for the advantages that metallosupramolecular entities can provide—the major one is their encapsulation and delivery potential in addition to the intrinsic properties of their building components (Therrien et al., 2008; Schmitt et al., 2012; Therrien, 2013; Ma and Zhao, 2015).

As a multidisciplinary field, the topic of biomedical application of metallosupramolecules requires mutual understanding between synthetic chemists and biologists with the continuing help of specialist in photochemistry, pharmacology, bioimaging, etc. With the ultimate goal to facilitate this process, we intended to describe the recent achievements in the design of metallacycles, metallacages and helicates, and metallosupramolecular capsules and barrels in view of their anticancer properties, recognition of biomolecules with therapeutic implication, as well as improved delivery of bioactive molecules for therapeutic or imaging purposes. The examples of advanced nanomaterials that are based on, or incorporate, metallosupramolecular entities have grown in number and enriched in functionality, and are, therefore, given a major importance in this review paper. While the specific areas of biological applications of the metal-based supramolecules are described, the effect of their characteristic structural features is emphasized. This includes the charge, the shape and the size of the metallosupramolecules, as well as their specific cavity, which reflect the overall stability, reactivity, encapsulation potential, etc.—all being related to the biologically relevant applications. Ultimately, the wide variety of structures with desired composition and properties that can be suitably obtained through supramolecular coordination chemistry, makes this class of compounds a generous area for exploration not only from synthetic point of view but also in view of their biological activity.

Metallacycles

As two-dimensional structures, the properties of metallacycles can be tuned by mere combination of the intrinsic characteristics of the metal ion and the linking organic ligands, thus forming supramolecular entities of variable size, shape, and charge. The stability issues have been overcome by employing inert metal ions as metal hinges or organometallic clips that form more robust metal-carbon bonds. Among the latter are the half-sandwich Ru(II) arene clips with bridging oxalate ligands that have been developed by Süss-Fink and coworkers and employed in the construction of the first organometallic Ru(II) metallamacrocycles (Yan et al., 1997). As the anticancer properties of ruthenium complexes came into focus after their introduction into clinical trials, and the first reports on the in vitro and in vivo cytotoxicity of a series of novel Ru(II) organometallic arene complexes (Aird et al., 2002), the topic expanded further on to the anticancer potency of metallosupramolecular assemblies with the pioneering works of Therrien and coworkers (Therrien et al., 2008; Mattsson et al., 2009). Several groups had greatly contributed in the field of biological applications of metallacycles of different shape and composition. The anticancer properties of series of tetracationic metallacycles, formed by [2+2] assembly between dipyridyl ligands and Ru(II) arene clips with oxalate or dioxydo-1,4-quinonato types of bridges of different size, have been thoroughly studied and later reviewed by Cook, Stang and coworkers (Cook et al., 2013). While this class of organometallic Ru(II) metallacycles demonstrated promising cytotoxicity profiles and structure-activity relationships could be outlined, the bis-ruthenium arene clips played further role in building 3D supramolecular cages and boxes by coordinating with tripodal or tetrapodal pyridyl ligands. Thereby, additional functionality has been introduced in terms of guest-encapsulation capability that further empowered the biological potential of the organometallic arene-metallosupramolecules. This topic has been extensively reviewed (Zhang et al., 2017) and therefore shall be left outside the scope of this paper.

Pt(II) and Pd(II)-cornered square-planar complexes have long been utilized in building highly symmetric 2D and 3D structures of desired shape and size. As chemically more inert units, the ethylenediamine-Pt(II) corners have been used to form [2+2] self-assemblies with rhomboidal structures by coordinating with bent-shape bipyridyl ligands. Some recent examples utilized the cationic N-monoalkylated 4,4′-bipyridinium or 2,7- diazapyrenium based ligands (Terenzi et al., 2012; Domarco et al., 2017) depicted in Figure 1. Similarly to earlier reports on DNA-binding capability of tetranuclear Pt(II) squares of linear bipyridyne ligands (Mounir et al., 2007), the formed hexa- and octacationic Pt(II) rectangular metallacycles strongly bind native and G-quadruplex DNA. While the smaller size, unsymmetrical 2,7- diazapyrenium ligand is able to intercalate the DNA, the formed metallacycle (1) binds DNA ten times more strongly and bends its structure, thereby blocking the DNA transaction that possibly reflects on the observed anticancer activity against four types of human cancer cell lines (Terenzi et al., 2012). Similarly, the octacationic Pt(II)-cornered metallacycles 2a-c exhibit moderate anticancer activity as compared to cisplatin (Table 1), which has also been related to their strong binding to DNA (Domarco et al., 2017). Interestingly, the size of the rectangular box determines their effect on the DNA binding and the G4 folding process—the smaller Pt-box exhibited lower binding affinity but better selectivity for a particular G4 topology. The middle-sized box 2b showed highest anticancer activity against the tested cancer cells, including the cisplatin resistant cell line VM-1, which, however, can be associated with the detected moderate toxicity of the free ligand (L3) against the U2OS cells (38.2 ± 3.4 μM).

FIGURE 1

Figure 1. Metallosupramolecular rhomboids and triangles based on cis-blocked Pt(II) and Pd(II) corners and bent shaped or linear donors.

TABLE 1

Table 1. Cytotoxicity data of selected metallacycles against a panel of human cancer cells.

More recently, new types of cornered Pt(II) and Pd(II) square-planar complexes (A1-A3, Figure 1) have been utilized in building metallosupramolecules with potential biomedical use. Notable examples are the highly fluorescent triangles constructed from boron dipyrromethene (BODIPY) based dipodal ligands and Pt(II) or Pd(II) corners, as they combine cellular imaging and cancer treatment potency of the building components. The highly emissive BODIPY-Platinum supramolecular triangles (3 and 4 in Figure 1) proved potent for intracellular visualization by confocal microscopy as well as for photodynamic therapy (PDT) and chemotherapy (Zhou et al., 2018a). Indeed, these metallacycles were used in the form of nanoparticles (NPs) that have been prepared through nanoprecipitation. More favorable cellular internalization of the NPs, formed from the cationic metallacycles 3 and 4, has been observed in comparison with the free, pyridine functionalized BODIPY ligand. Furthermore, metallacycles 3 and 4 demonstrated strong anticancer activity against HeLa cells, which is intrinsic property of the Pt(II) corners A1 and A2 (Table 2). By light irradiation, however, the anticancer activity could be further amplified through inducing the photodynamic therapy modality, which is characteristic property of the BODIPY fluorophore, and have resulted in significant enhancement in the cytotoxicity against the cisplatin resistant cell line A2780cis. Thereby, effective theranostic agents have been obtained through combination of the chemotherapeutic potential of the platinum acceptors with the BODIPY donors as imaging probes and photosensitizers.

TABLE 2

Table 2. Cytotoxicity data of selected metallacycles against a panel of human cancer cells.

In another work, BODIPY derived Pd(II) metallosupramolecular triangles have been obtained by using Pd(II) hinges that are cis-capped with 1,3-bis-diphenylphosphino-propane, and the triangle-to-square conversion was studied (5a-d in Figure 1) (Gupta et al., 2017). The structures were fully characterized by ¹H, ³¹P NMR, ESI-MS, and X-ray diffraction (of the triangle). The triangle and square topologies exist in a solvent-dependent equilibrium with the triangles being favored in higher polarity solvents. The stability of the complexes has been evaluated by UV-Vis absorption in presence of chlorides and in phosphate-buffered solution in DMSO or Dulbecco's Modified Eagle Medium, showing no change in the spectra for up to 48 h, except for the partial disruption seen upon addition of chloride ions. These BODIPY derived Pd(II) supramolecular triangles showed selective cytotoxicity against brain cancer (glioblastoma) cells U87 in comparison to that against normal brain cells WI-38. The anticancer activity of the triangles is higher or comparable to that of cisplatin, whereas the estimated selectivity is less pronounced than the selectivity of cisplatin (Table 2). These green fluorescent metallacycles have also been tested for fluorescence imaging of the cancer cells; the data demonstrated internalization in the cytoplasm and accumulation of aggregates in the cell membrane but not in the nucleus. Unlike the smaller precursors, the supramolecular triangles show appreciable interaction with the tested biomolecules—dsDNA, ssDNA, and BSA.

Hexagonal metallamacrocycles have been obtained by self-assembly of an organometallic platinum(II) clip A4 with linear dipodal ligands, 4,4′-bipyridine or pyrazine, depicted in Figure 2 (Bhowmick et al., 2018). Both macrocycles (6a and 7) exhibited cytotoxicity higher than cisplatin against the human breast cancer cell line MCF7. In general, the smaller-size macrocycle 6a showed higher anticancer activity against all cell types (Table 3), and induced higher cell membrane damage. The latter has been associated with eventual necrosis of the cancer cells, in addition to the apoptosis, which was related to the swollen morphology of the treated cells seen by fluorescence microscopy imaging. The difference in the observed anticancer activity of the studied macrocycles has been associated with the lower hydrophobicity and lower molecular weight of 6a; stability in presence of biomolecules has not been tested. Similarly to the macrocycles 6a and 7, the synthesis and anticancer potency of other flexible [2 + 2] tetracationic metallamacrocycles have been estimated (Jana et al., 2018). The macrocycle 6b exhibited moderate anticancer activity against the tested cell lines (Table 3) as a result of self-assembly of the non-toxic organometallic clip A5 and the pyrazine ligand. Yet, its cytotoxicity is lower than cisplatin and no selectivity could be attained.

FIGURE 2

Figure 2. Metallacycles built from organoplatinum(II) acceptors and bent shaped ligands.

TABLE 3

Table 3. Cytotoxicity data of selected metallacycles against a panel of human cancer cells.

Recently, two supramolecular metallacycles with hexagonal and rhombic shape have been obtained by using pyrazine-based organoplatinum(II) clip A6 and two isomeric, also pyrazine-based, ditopic pyridyl ligands (Jana et al., 2019). The para- isomer of the ligand L7 is shown in Figure 2 and leads to formation of the hexagonal metallacycle 11 upon [3+3] self-assembly with the pyrazine-based organoplatinum(II) acceptor, whereas the meta- isomer of L7 forms a rhomboidal structure (not shown) upon [2+2] self-assembly with the same organoplatinum(II) acceptor. Both types of metallacycles showed slightly higher anticancer activity than the organoplatinum(II) acceptor A6 and cisplatin (Table 3), which is more appreciable for the larger, hexacationic [3+3] self-assembly 11.

The rhomboidal Pt(II)-metallacycle 9, depicted in Figure 2, has been evaluated for its anticancer activity both in vivo and in vitro (Grishagin et al., 2014). The formed metallacycle shows 13 times higher emission in the visible region (400–700 nm) than the parent ligand in aqueous-DMSO solution. This property has been used to monitor the cellular uptake and the stability of the metallacycle by laser-scanning confocal microscopy in A549 and HeLa cells. The data evidenced their stability upon cellular internalization in addition to the high resistance to photobleaching under the conditions of the confocal microscopy experiment. At low concentrations (1 nM−5 μM) the rhomboidal metallacycle and the organoplatinum(II) acceptor are not toxic, as seen by MTT test after 48 h of treatment of the cancerous cells. However, in the in vivo experiments in immunocompromised nude mice with tumor xenografts of breast cancer MDA-MB-231, the rhomboid 9 exerted a median tumor volume reduction of 88% (P < 0.001) (Figure 3). A maximum dose of 6 mg/kg in PBS:DMSO = 1:1 (v/v) has been applied by i.p. injections of 300 μL per animal, leading to no obvious signs of toxicity in terms of weight loss and visual appearance and behavior of the treated mice. The tumor growth inhibition (T/C%) value, defined as the ratio of the median tumor volume for the treated vs. the control group at a particular day, has been estimated to 36% at the last day of the experiment of 30 days (Figure 3 left).

FIGURE 3

Figure 3. Effect of rhomboid 9 on tumor growth rate in MDA-MB-231 xenografts. (Left) Representative pictures of tumors, excised from control and mice treated with rhomboid 9. (Right) Localization of the near-infrared contrast agent IR-783 in the tumors of the control and treated mice. The signal was processed with Living Image software with one representative sample for each group presented above. Mice from the rhomboid 9-treated group show lower intensity of the signal originating from the tumor-accumulated contrast agent compared with the control group. Adapted with permission from Grishagin et al. (2014).

Stang and coworkers prepared metallosupramolecular polymers based on an organometallic Pt-linked rhomboidal metallacycle 8b (Figure 2) that was conjugated with two types of polymers via amidation reaction between alkylamine and N-hydroxysuccinimide-activated carboxylic acid (Zhang et al., 2016). These metallacycle-cored polymers P1 and P2 (Figure 4) have been utilized to form nanoparticles at low concentration. All synthetic modifications resulted in enhanced emission due to aggregation-induced emission properties of the tetraphenylethene-based pyridyl donor that is the main building block of the metallacycle. The fluorescence properties of these polymers were further used in cell imaging, which showed a significant enrichment in lung cells after i.v. injection into a mouse bearing an MDA-MB-231 tumor. The observed significant fluorescence of the tumor even 24 h after injection demonstrated that the studied metallosupramolecular polymer (P2) is both chemostable and photostable in vivo. Considering the anticancer activity of previously reported rhomboidal Pt(II) metallacycles of Grishagin et al. (2014), the authors infer that this type of fluorescent metallacycle-cored polymers can have potential applications in lung cancer theranostics.

FIGURE 4

Figure 4. Polymeric networks in P1 and P2 built by interconnecting metallacylces 8b. Adapted with permission from Zhang et al. (2016).

The aggregation-induced emission could also be exploited in building metallosupramolecular species as has been described for a hexagonal [3+3] self-assembled metallacycle 10 built from tetraphenylethylene (TPE)-based organoplatinum(II) acceptors and the ditopic TPE-based dipyridyl ligand L6 (Figure 2), as well as that of octacationic cages that were obtained from a tetrapodal tetrapyridyl ligand similar to L6 (Yan et al., 2016). Interestingly, both types of supramolecular assemblies showed distinct photophysical properties depending on the degree of flexibility that the ligand attains after self-assembling with the TPE-based platinum(II) acceptors. The hexacationic metallacycle 10 exhibits aggregation-induced emission (AIE) by increasing the amount of hexane in the CH₂Cl₂ solutions (up to 90%) due to the formation of nanoaggregates, as seen from the scanning electron microscopy (SEM) images, whereas the cages are intrinsically highly emissive. Taking advantage of the observed aggregation-induced enhanced emission (AIEE) of these systems, the same authors applied advanced approaches to build hybrid metallosupramolecular polymeric and bio-nanoparticles. The hexacationic metallacycle 10 has been assembled with the negatively charged rod-like tobacco mosaic virus (TMV) to form three-dimensional micrometer-sized superstructures via electrostatic interactions (Tian et al., 2016). In this way the fluorescence dramatically increased and provided an easily detectable evidence for the formation of novel metallosupramolecular biohybrid materials. Interestingly, addition of tetrabutylammonium bromide disrupts the metallosupramolecular core which results in lower fluorescence. The same strategy has been used to build similar biohybrid materials with bacteriophage M13 and ferritin, although with different degree of aggregation-induced emission. The demonstrated fluorescence switching upon aggregation with negatively charged protein-based species, forming emissive bionanoparticle/metallosupramolecular hybrid complexes, and their controllable disruption, enrich significantly the possibilities for design and development of different supramolecular assemblies with dynamic optical properties and high potential to be used for both imaging and delivery of bioactive compounds.

On the other hand, aggregation-induced quenching may significantly compromise the photophysical functionality of some fluorophores such as porphyrins. In a recent paper, an alternative way to produce porphyrin functionalized hexagonal metallacycle has been reported (Chen et al., 2018). To avoid self-aggregation of the porphyrin rings, the metallacycle depicted in Figure 5 has been produced in the confined cavity of mesoporous carbon FDU-16. Thereby, the molecules of the porphyrin-containing metallacycle have been dispersed within the mesoporous cavities, which resulted in increased stability and photocatalytic activity of the porphyrin-containing metallacycles. These properties have been demonstrated through successful use of the hybrid material in heterogeneous catalysis for photooxidation of sulfides. The six-fold faster generation of singlet (¹O₂), compared to that of free metallacycles in solution, is an important improvement that has been achieved. Although no biological applications have been discussed yet, the demonstrated strategy to construct well-defined porphyrin-containing metallacycles with improved stability and photoactivity may find implications in the future development of nanohybrids with improved properties for PDT.

FIGURE 5

Figure 5. Construction of porphyrin-containing metallacycle within the confined cavity of mesoporous carbon FDU-16. Adapted with permission from Chen et al. (2018); Copyright 2018 American Chemical Society.

Another strategy to build metallosupramolecules with significantly augmented photophysical properties, which allow for their use in photodynamic therapy, has recently been demonstrated for a heterometallic Ru-Pt metallacycle 12 (Figure 6) (Zhou et al., 2018b). The authors utilized the excellent photostability and two-photon absorption characteristics of the Ru(II) polypyridyl precursor, which after formation of the metallacycle exhibits shifted luminescence to the near-infrared region characterized also with a larger two-photon absorption cross-section and higher singlet oxygen generation efficiency. Thereby, the formed large macrocyclic structure of 12 renders it a very potent two-photon PDT agent. It is proposed that the highly charged (decacationic) structure of 12 facilitates its internalization in cells and localization in mitochondria and nuclei as evidenced by time-dependent inductively coupled plasma MS (ICP-MS) analysis of cellular uptake and the biodistribution of 12 in A549 cells. The metallacycle exhibits very high photocytotoxicity, induced by the generation of intracellular ¹O₂ upon irradiation and damaging the mitochondria and cellular nuclei, whereas the toxicity in the dark is very weak. These observations have also been confirmed in the in vivo studies which demonstrated that 12 is capable to efficiently ablate cancer tissues at low light doses with minimal system toxicity.

FIGURE 6

Figure 6. Heterobimetallic cycles 12 – 14 with notable optical, electrochemical, and anticancer properties.

The tetracationic heterobimetallic cycles 13 and 14 have been obtained from an N, N′-bis(4-(pyridin-4-ylethynyl)phenyl)pyridine-2,6-dicarboxamide ligand and cis-blocked palladium(II) or platinum(II) 1,1′-bis(diphenylphosphino)ferrocene triflates (Mishra et al., 2014). Their structure and composition have been characterized by multinuclear NMR spectroscopy, elemental analysis, and high-resolution electrospray mass spectrometry (HR-ESMS) and further modeled by molecular mechanics calculations. The stability of the metallacycles has been tested either by monitoring the cytotoxicity effect after different periods of pre-incubation in cell culture medium (Dulbecco's modified eagle medium—DMEM) or in DMSO (for 0, 10, 25, and 50 h at 37 ⁰C) or by fluorescence spectroscopy measurements in physiological buffer solution at pH 7.4 and in presence of redox active compounds (H₂O₂ or dithiothreitol). While both metallacycles exhibit cytotoxicity higher than cisplatin against the tested human-cancer cell lines (T98G, KB and SNU-80), they are twice less toxic to the normal human embryonic kidney cells (HEK-293) than cisplatin, which points to a much better cancer cell selectivity (Table 3). The stability studies indicated that under physiological conditions and even in presence of redox-active compounds the metallacycles 13 and 14 remain intact for at least 50 h, whereas in presence of DMSO a significant loss of cytotoxicity has been seen for pre-incubation periods of > 25 hours. These observations could suggest that the metallosupramolecular structure of 13 and 14 is crucial for the observed cytotoxicity profiles, since the DMSO induced disassembly is proposed as the main reason for decrease in the cytotoxicity. The mode of action leading to the observed cytotoxicity has been associated with apoptosis as evidenced by the caspase-3/7 activity assays.

Metallacages and Helicates

Metallacages and helicates are 3D structures that have characteristic cavity or cylindrical shape. Thereby, except combining the properties of the building components, metallacages can be excellent hosts for a variety of guest molecules that can be encapsulated in the hollow of the cages. This particular feature of metallacages has long been utilized in the design and development of smart nanostructures for multiple applications, such as molecular flasks (Yoshizawa et al., 2009), containers (Zarra et al., 2015), for safe storage (Yamashina et al., 2014) and recognition (Rodriguez et al., 2017), etc. Metallahelicates, on the other hand, can have specific helicity that is capable of selective interactions with biomolecules with certain conformation, such as DNA fragments. The growing interest in exploring the biomedical applications of these types of 3D metallosupramolecules has progressed to building metallacages that are either capable of encapsulating bioactive molecules or can be structurally decorated with biofunctional fragments. Thereby, the areas of potential applications of metallacages already include drug delivery and bioimaging except the initially explored biological activities that are intrinsically characteristics for their components. Similar expansion in the exploration of the properties of metallahelicates is also in progress.

Some examples of metallacages that had attracted the attention of synthetic and bioinorganic chemists are the [Pd₂L₄]⁴⁺ cages that assemble from simple ditopic tripyridyl ligands−2,6-bis(pyridin-3-ylethynyl)pyridine or its bis(pyridin-3-ylethynyl)benzene analog (Figure 7). The interest has been initiated with the reported encapsulation of the anticancer drug cisplatin by the supramolecular cage 15 that can be controllably released by external stimuli (Lewis et al., 2012). The cage formation and cisplatin encapsulation proceeds virtually quantitatively and the cage 15 and its host-guest complex with encapsulated two molecules of cisplatin have been characterized by single-crystal X-ray diffraction analyses. While the cage and the host-guest complex are rather stable in organic solvents, the cage readily disassembles in presence of competing amine ligands or chlorides, and thus the encapsulated cisplatin molecules can be released. The authors studied in details the encapsulation phenomenon and highlighted the importance of the hydrogen bonding interactions between the cage and the amine ligands of the cisplatin guest. This conclusion has been suggested by the lack of encapsulation evidences when a Pd₂L₄ metallacage built from 1,3-bis(pyridin-3-ylethynyl)benzene ligand (when X = CH in 15, Figure 7) has been used.

FIGURE 7

Figure 7. Metallosupramolecular cages with general formula [Pd₂L₄](BF₄)₄ built from a series of tri- or bi-pyridyl ditopic ligands.

Further exploration of the observed stimuli-responsive catch and release of cisplatin by cage 15 has been focused on suitable ligand modifications to include an optically responsive fragment and/or increase the stability of the cage and the host-guest complex under biologically relevant conditions. The Cu(I)-catalyzed azide-alkyne cycloaddition “click” reaction (CuAAC) has been utilized to build a library of electrochemically or photochemically responsive cages (16a-c, Figure 7) (Lewis et al., 2013, 2014). It has been demonstrated that the formed 1,2,3-triazole units in the exo-functionalization does not affect the self-assembling process nor does it interfere with the ability of the formed cages to bind cisplatin as guest molecules. Similarly, single-crystal X-ray structure has been determined for the 1:2—host-guest complex of cage 16a and cisplatin (Lewis et al., 2013). The cage formation does not alter the one-electron reversible oxidation characteristic for 4-ferrocenyl triazoles. The same strategy has been used to “click” photoresponsive or biological molecules, such as 4-amino-1,8-naphthalimide (in 16b, Figure 7) and fac-[Re(CO)₃] complex with the 4-(2-pyridyl)-1,2,3-triazolyl moiety (in 16c, Figure 7) or caffeine, acetate protected-D-glucose, estradiol and dipeptide units (Lewis et al., 2014). The attachment of the organic or inorganic fluorophores (in 16b and 16c, respectively) transformed the virtually non-emissive cages into emissive ones, with especially efficient emission seen for cage 16b showing quantum yield of fluorescence of 0.59.

With the ultimate goal to improve the kinetic stability of the studied Pd₂L₄ cages and evaluate their biological activity, Crowley and coworkers further modified the used tripyridyl ligands by adding NH₂-susbstituents in the terminal pyridyl rings (Preston et al., 2016). Thereby, cages 17a and 17b have been obtained and their kinetic and biological properties have been compared with the non-functionalized cage 18d. The stability studies showed that 2-amino substitution on the terminal pyridine rings significantly enhances the stability of the formed cage 17a in presence of chloride ions or histidine and cysteine—potent ligands in biological environment. Interestingly, cage 17b has kinetic properties and cisplatin-encapsulation ability similar to that of 18d, which points out to the importance of subtle structural modification of the self-assembling ligands on the properties of the formed metallacages. Enhanced stability of 17a of up to several hours to almost 2 days (in presence of histidine) reflected also to the observed appreciable cytotoxicity against the cisplatin-resistant breast cancer cell line MDA-MB-231 (Table 4). In contrast, the 17b and 18d did not show appreciable cytotoxicity against the tested lung and breast cancer cell (for 24 h of incubation), which can be in favor to their stimuli-responsive cisplatin encapsulation and the suggested drug-delivery potential.

TABLE 4

Table 4. Cytotoxicity data of selected metallacages against a panel of human cancer cells (Figure 7).

Further functionalization and cytotoxicity studies on this class of metallacages have been undertaken by Casini, Kühn and coworkers. The antiproliferative effects of Pd(II) cages of hydroxymethyl-functionalized bi- and tripyridyl ligands (18c and 18d in Figure 7, respectively) indicated that upon 72 h of incubation the cages exert cytotoxicity against the tested cancer cells either comparable to that of cisplatin (for 18c) or 4-times lower (for 18d, Table 4) (Schmidt et al., 2016b). The other two examples of Pd(II) cages derived from differently functionalized bipyridyne ligands (18a and 18b in Figure 7) have the same order of cytotoxicity effect as 18c. The observed lower toxicity of 18d and its distinctive ability to encapsulate cisplatin, as suggested earlier by Crowley and confirmed herein by X-ray data of the host-guest complex, can be regarded as favorable combination of properties for the sake of anticancer drug delivery potential (Schmidt et al., 2016b). To introduce optical functionality to the observed higher cytotoxicity of the Pd(II) cages derived from the bi-pyridyl ligands, the same authors exo-functionalized the Pd₂L₄ cages with naphthalene or anthracene fluorophores (Schmidt et al., 2016a). The formed ligands exhibit weak fluorescence and upon self-assembly and formation of the cages 19a – 19d (Figure 7) the emissive properties of the fluorophore-tagged ligands have been preserved leading to the formation of weakly or non-emissive metallacages, most probably due to the high degree of flexibility. The exo-functionalization with aromatic rings, however, reflected the cytotoxicity of the ligands and the corresponding metallacages 19a, 19b, and 19d that increased considerably when compared with the non-functionalized cage 19c (Table 4). Interestingly, by introducing methoxy groups in the terminal pyridine rings the fluorescence of the ligands increases (Kaiser et al., 2016); upon capsule formation, however, virtually non-emissive Pd(II)- and a novel Pt(II) cage have been obtained. Interaction of the Pd(II) cages of metoxy-functionalized bi- and tri-pyridine ligands with cisplatin has been suggested by ¹H NMR measurements. Pd(II) cage 20 and its analog of a bi-pyridine ligand (structure not shown) have been proposed as suitable drug-delivery platforms as the estimated cytotoxicity is relatively low.

To achieve efficient targeting, functionalization of metallacages with biological fragments has been reported for the Pd₂L₄ cages of the di-pyridyl ligands depicted in Figure 7 (Han et al., 2017). Two different approaches for exo-functionalization with a model linear peptide Ac-NLEFK-Am have been described, which are based on amide bond formation between the carboxylic (or amine) group of the exo-functionalized ligand/cage and that of the side chains of the model peptide. Better results could be obtained with the pre-conjugation approach, where the peptide is coupled to the ligand prior to the following in situ self-assembly and formation of the Pd₂L₄ cages. Thereby, metallacages of larger size could be obtained that can more efficiently benefit from the enhance permeability effect (EPR) for passive targeting to cancer cells and tissues. More importantly, this approach could open the prospect to bioconjugation and specific targeting of metallosupramolecules by attaching receptor-specific peptides or antibodies as an way to complement their either drug-delivery potential or intrinsic cytotoxicity (Han et al., 2017).

Metallosupramolecular systems of composition [Pd₂L₄]⁴⁺ have been isolated by using smaller size ditopic ligands based on bis-triazoles similar to L9 in Figure 8 (McNeill et al., 2015). The formed complexes have the form of quadruply-stranded helicates similarly to the crystal structure of 21, depicted in Figure 8 and reported earlier (Crowley and Gavey, 2010). These helical structures have improved stability that is much more pronounced in the case of bis-triazole ligand with hexyl pendant arms forming the analogous complex 21-hex (structure not shown). The increased stability in presence of chlorides, histidine and cysteine correlated with the observed higher cytotoxicity against the tested cancer cell lines in comparison of the metallacage 15 (Table 4). Interestingly, the complex 21-hex attains its half-inhibitory concentration the first 2 h of treatment of the cells in contrast to the known anticancer platinum drugs. This suggested different mode of anticancer action that has been associated with loss of cellular membrane integrity as derived from fluorescence microscope imaging and enzymatic (LDH) activity assay (McNeill et al., 2015). This hypothesis also corroborated with observed lack of selectivity of the cytotoxicity of complex 21-hex. A large library of bis(bidentate) 2-pyridyl-1,2,3-triazole ligands with various lengths have been explored for their ability to form triple helicates with Fe(II) ions with general composition [Fe₂L₃]⁴⁺ (Vellas et al., 2013). While self-assembly and formations of cylinders have been confirmed in solution as well as in the solid state, the poor stability of these complexes hampered their potential biological activity. Molecular docking studies of these complexes suggested they could bind duplex and triplex DNA fragments.

FIGURE 8

Figure 8. Dipodal ligands (L9 – L13) for building helical metallosupramolecules similarly to represented crystallographic views of selected examples (21 – 25). Views of the crystal structures are produced with Mercury3.8 software from the corresponding files: 21 – CCDC 762916; 22 – CCDC 603391; 23 – CCDC 603392; 24 – CCDC 636261; 25 – CCDC 1566897; all are freely available from www.ccdc.cam.ac.uk.

The chiral metallohelical complexes [Fe₂(L10)₃]⁴⁺ and [Fe₂(L11)₃]⁴⁺, constructed by Scott, Qu and coworkers, have shown evidences to selectively target and enantioselectively inhibit amyloid-β (Aβ) aggregation (Li et al., 2014). The ability of these metal complexes to inhibit Aβ assembly suggested that they might be useful in blocking Aβ-mediated cellular toxicity and thereby present a therapeutic strategy for treatment of Alzheimer's disease. The selective targeting of alpha/beta-discordant stretches at the early steps of aggregation has been demonstrated by multiple biophysical and biochemical approaches. More recently, a series of analogs of [Fe₂(L11)₃]⁴⁺, having linking bridges of different flexibility, have been tested for their anticancer activity and evidenced both very good water solubility and stability (Kaner et al., 2016). Interestingly, the so-called flexicates exhibit anticancer activity of sub-micromolar to nanomolar range against the tested human cancer cell lines: MDA-MB-468 (human epithelial breast adenocarcinoma), HCT116 p53^+/+ and HCT116 p53^−/− that are genetically identical human colorectal cancer cell lines except for the presence or absence of functional p53, which is a common genetic difference associated with increased resistance to chemotherapy. In the cisplatin-sensitive cells MDA-MB-468, the glycolbridged flexicate analogous to [Fe₂(L11)₃]⁴⁺ have shown a cytotoxicity (IC₅₀ = 0.2 ± 0.1 μM) higher than cisplatin by an order of magnitude. Moreover, some of the tested flexicates were very toxic against the resistant HCT116 p53^−/−cells that lack p53 and showing cytotoxicity as low as 40 nM. Another important feature of this class of flexicates is their excellent selectivity, which is estimated to be nearly three orders of magnitude higher than that of cisplatin against healthy human retinal pigment epithelial (ARPE19) and lung fibroblast (WI38) cells. The helical complex [Fe₂(L10)₃]⁴⁺ showed limited solubility in water and could not be fully characterized, whereas its m-xylenyl analog exhibited cytotoxicity as high as 0.4 ± 0.1 μM against the MDA-MB-468 cell lines. In some cases significant enantiomeric difference had been observed pointing to the structural and conformational importance of the helicates. Nevertheless, the investigated mode of action could not be associated with DNA interaction nor apoptosis induced cell death.

The earlier reported metallosupramolecular helicate [Fe₂(L12)₃]⁴⁺ of Hannon and coworkers are capable of binding DNA Y-shaped junctions and could also unwind DNA and bind preferentially to regular alternating purine–pyrimidine sequences (Malina et al., 2008). More recent studies on the DNA interaction preferences have also revealed that this metallohelicate is capable of recognizing DNA bulges that are DNA defects consisting of one or more unpaired bases in the DNA double helical strands (Malina et al., 2014). These findings complemented the available data on the ability of the [Fe₂(L12)₃]⁴⁺ helicate to recognize non-canonical structures in DNA that might have relevance with various diseases. Recent report on the ability of the [Fe₂(L12)₃]⁴⁺ helicate to bind to TAR RNA, which is transactivation responsive region RNA, suggested it biological potential of inhibiting an essential step in the HIV-1 replication cycle (Malina et al., 2016). The observation that the iron(II) supramolecular helicates binds to TAR RNA at nanomolar concentrations and inhibits the interaction between the HIV-1 transactivator protein Tat and TAR, which plays a critical role in HIV-1 transcription, broadens the scope of biological importance of metallosupramolecular helicates.

The anticancer potency of dinuclear ruthenium metallohelicates have been among the first examples of biological activity demonstrated for double-stranded (22 and 23 in Figure 8) (Hotze et al., 2006) and triple-stranded (24 in Figure 8) metallohelicates (Pascu et al., 2007). The two different isomers of the dinuclear [Ru₂L₂Cl₄] complex exhibit slightly different cytotoxicity profiles (Table 4) with the symmetric 23 isomer being an order of magnitude more cytotoxic than the cis/trans isomer 22 against the tested cancer cell lines. The analogous triple-stranded helicate 24 (Pascu et al., 2007) demonstrated very high stability and capability of binding DNA. This cylinder is photoresponsive to additions of ct-DNA in terms of fluorescence enhancement from the ruthenium cylinder and a concomitant blue shift (8 nm) in the emission maximum. The observed cytotoxicity, however, is significantly lower than the double-stranded analogs and also lower than that of cisplatin (Table 4). More recent examples on the construction and biological activity of double-stranded Ru(II) helicates of L13 have been reported by Rice and coworkers and suggested the formed helicate 25 as a suitable compound that can target difficult to treat tumors (Allison et al., 2018), such as the human colorectal cancer cell line HCT116. The selectivity of 25 was similar to cisplatin and oxaliplatin toward the HCT116 p53^+/+ cancer cells, but it also showed substantively higher cytotoxicity toward the p53^−/− cells.

Advanced approaches that utilize metallosupramolecular cages as delivery system for either anticancer drugs and prodrugs or fluorescent agents have gained increasing interest as alternative ways to achieve selectivity and efficient targeting of tumor tissues for both therapeutic and imaging purposes. Among the initial examples are those that use the hexanuclear octahedral metallacage of Fujita (Pt-cage 1 in Figure 9), (Ibukuro et al., 1998), which has been shown to encapsulate four molecules of adamantyl Pt(IV)-complex exhibiting prodrug properties (Zheng et al., 2015). The formed host-guest complex has a diameter of about 3 nm, as characterized by detailed NMR analyses. The cytotoxicity of the host-guest complex is much higher than these of the Pt-cage 1 or the Pt(IV)-prodrug guest alone, and is comparable to the anticancer activity of cisplatin against a panel of human cancer cells lines. The improved anticancer activity is caused by biological reductions of the Pt(IV)-prodrug, as evidenced by adding ascorbic acid, and release of the cytotoxic cisplatin. Thereby the cisplatin resistance in the tested A280CP70 cell lines could be overcome that was explained with the high cellular uptake of the highly positive charge of the host-guest nanostructure. On the other hand, by changing the type of the Pt(II)-cornering complexes of Pt-cage 1, from ethilenediamine to an 1,1′-bipyridine complex, the formed octahedral cage becomes more cytotoxic than cisplatin against a panel of human cancer cells including the cisplatin-resistant celss (IC₅₀ = 3.42 ± 0.63 μM for A2780CP70 and IC₅₀ = 7.85 ± 0.21 μM for MD-MBA-231 vs. IC₅₀ values for cisplatin of 6.49 ± 1.40 μM for A2780CP70 and 15.4 ± 2.3 μM for MDA-MB-231) (Zheng et al., 2016). The detailed study on the mechanism of action of this Pt₆L₄ octahedral cage showed that it interacts with DNA in a non-covalent intercalative way leading to DNA damage, and induces apoptosis through upregulation of p53 and p21 proteins.

FIGURE 9

Figure 9. Advanced metallo supramolecular systems constructed from platinum metallacages. (A) Pt(IV) adamantyl prodrug encapsulated in the rhombic Pt-cage 1 (adapted from Zheng et al., 2015; Published by The Royal Society of Chemistry); (B) hybrid metallosupramolecular-polymeric nanoparticles built from host-guest complex of fluorescein-conjugated Pt(IV) prodrug and Pt-cage 1 coated with anionic polymer shell (adapted from Yue et al., 2018; with permission from RSC); (C) theranostic supramolecular nanoparticles built from highly emissive metallacage, Pt-cage 2, and biotin-conjugated PEGylated surfactant (adapted from Yu et al., 2016).

Another strategy has been tested in building drug-loaded nanoparticles with polymeric shell of anionic block copolymer (methoxy polyethylene glycol-blockpolyglutamic acid, MPEG5k-PGA30) (Yue et al., 2018). The same octahedral metallacage (Pt-cage 1) has been used to encapsulate a fluorescein-linked Pt(IV) prodrug (Figure 9B). The Pt(IV) prodrug-loaded supramolecular nanoparticles have spherical shape, and their average size was estimated to 20 nm by cryo-TEM imaging or overall size of 80 nm including the peripheral polymers and according to the dynamic light scattering (DLS) data. The drug-release profiles speeds up dramatically under acidic conditions (pH = 5.0 in acetate buffer) and reducing environment of 1 mM ascorbic acid, that is estimated by the release half-life being 3 days in PBS and only 23 h in acidic and reducing environment. Intracellular release of the fluorescein-conjugated Pt(IV) prodrug was evidenced by fluorescence microscopy that has been enabled by the appearance of bright green fluorescence of the prodrug released from the otherwise non-fluorescent nanoparticle. Moreover, the cytotoxicity of the nanoparticles against HeLa cells is comparable to that of cisplatin (IC₅₀ = 5.02 ± 0.67 μM for the nanoparticles; 2.95 ± 0.42 μM for cisplatin, and 5.98 ± 0.58 μM for the Pt(IV) prodrug), whereas the metallacage (Pt-cage 1) is essentially non-toxic. This work demonstrates an working strategy to use anionic block copolymer, to include a cytotoxic host-guest complex into negatively charged nanoparticles of appropriate size that are able to slowly release the therapeutic content and show excellent anticancer efficacy accompanied with fluorescence imaging modality.

Alternatively, Stang and coworkers utilized a highly emissive tetraphenylethene metallacage to build supramolecular nanoparticles with 1,2-distearoyl-phosphatidylethanolamine (DSPE)/polyethylene glycol (PEG) shell (Figure 9C) showing excellent stability and biotin-receptor targeting ability to be used as cancer-cell selective delivery systems (Yu et al., 2016). Moreover, these supramolecular nanoparticles preserve the coordination triggered aggregation induced emission (AIE) enhancement, which renders them highly emissive in biological media and suitable for imaging purposes. The incorporated metallacage is built from Pt(II) linked triphenyl pillars, decorated with differently modified PEG-arms, and two tetraphenyl-ethene ligands each bearing four pyridines for coordination with the metal. The metal precursor is a cis-Pt(Et₃P)₂(OTf)₂ complex that exhibits high and unselective cytotoxicity. Upon formation of self-assembled polymeric aggregates with sizes of about 37 nm (as seen by TEM and DLS techniques) selective delivery to cancer cells has been achieved employing the EPR effect additionally to the biotin-mediated internalization in cancer cells. Thereby, the metallacage loaded nanoparticles combine the therapeutic with the imaging modality making them suitable for theranostic applications. Confocal laser scanning microscopy and flow cytometry confirmed the selective targeting of the biotin functionalized nanoparticles to cancer cells overexpressing the corresponding receptor (HeLa and HepG2 cells) in contrast to normal cells (CHO and HEK-293), which also corroborated with their higher and selective cytotoxicity and high Pt-content in the cancer cells nuclei. The theranostic functions of the biotin-functionalized nanoparticles have been demonstrated also in vivo in HeLa tumor bearing female nude mice confirming their superiority than the clinical formulations of oxaliplatin, carboplatin, or cisplatin used as control. Importantly, while higher accumulation of Pt from the nanoparticles was observed in the tumor in comparison with the control Pt-drugs, administration of nanoparticles resulted in a lower Pt uptake by the liver, spleen, lung, and kidneys, which suggested that the use of PEGylated nanoparticles also provides way to decrease the systemic toxicity of Pt-drugs.

Metallocapsules and Barrels

The design and utilization of large aromatic panels as multidentate donor ligands enabled the construction of 3D metallosupramolecular structures with a characteristic well-confined cavity, and having capsular or barrel-like shapes. Interesting examples consist of aromatic panels with intrinsic fluorescence properties, such as anthracene and carbazole fragments in the ligands depicted in Figure 10. The formation of robust M₂L₄ coordination capsules have been described by Yoshizawa and coworkers (Li et al., 2012), and their fluorescence and guest-encapsulation properties have been studied in details (Kishi et al., 2011) for the sake of practical applications for safe storage of reactive compounds (Yamashina et al., 2014) or recognition purposes (Yamashina et al., 2016, 2017).

FIGURE 10

Figure 10. Metallosupramolecular capsules 26 and 27 and barrels 28 and 29 with characteristic hydrophobic cavities.

Recent results on the anticancer properties of Pd(II) and Pt(II) capsules 26 and 27 (Figure 10) indicated that they exhibit anticancer activity profiles much superior to cisplatin in terms of higher toxicity against two types of leukemic cells (HL-60 and SKW-3) as well as against cisplatin-resistant cells (HL-60/CDDP) (Ahmedova et al., 2016b). More importantly, the capsules showed more than 5 times better selectivity toward cancerous cells than that of cisplatin, which has been estimated by the IC₅₀ of normal-to-cancer cell toxicity ratio employing human embryonic kidney cells (HEK-293) as a model for non-malignant cells. The stability tests revealed that both types of coordination capsules remain intact in presence of excess of biologically relevant molecules such as amino acids and DNA bases. However, the addition of sulfur-containing biomolecules such as cysteine, causes disassembly of the capsules that proceeds instantaneously in case of the Pd(II) capsule 26 and takes up to 3 days for the more inert Pt(II) analog 27. Decomposition of the capsules can be easily monitored by the appearance of the characteristic strong fluorescence of the liberated bis-anthracene ligand L16. Further on, the effect of guest encapsulation on the stability and the cytotoxicity of the capsules have been investigated (Ahmedova et al., 2016a). A successful modulation of these properties has been achieved by encapsulation of different types of hydrophobic guest molecules (pyrene and caffeine). The stabilities of the empty hosts and the corresponding host-guest complexes have been tested in presence of glutathione – the sulfur-containing oligopeptide that has relevance to cancer evolution. The observed strong stabilization of the capsules by encapsulation of pyrene guests has resulted in virtually complete loss of cytotoxicity of the otherwise highly toxic empty hosts 26 and 27 against the tested human cancer cell lines (HT-29, T-24, HL-60 and its resistant counterparts HL-60/Dox and HL-60/CDDP). These findings have suggested that the cytotoxicity of 26 and 27 can be readily modulated by encapsulation of large aromatic guests. More interestingly, the observed cytotoxicity profiles correlated with the capsules stability in the presence of glutathione that was estimated by NMR-based kinetic experiments. The observed stability-cytotoxicity correlation allowed proposing that the glutathione-triggered decomposition of the capsules could be the reason for the observed anticancer activity of these robust capsular metallosupramolecules. This hypothesis corroborates with the reported very high selectivity of the capsules to cancerous cells as well as with their cross-resistance cytotoxicity.

Mukherjee and coworkers reported the synthesis and single-crystal X-ray structure of a Pd₈L₄ water-soluble molecular barrel 28 that self-assembled from a tetrapyridyl donor (L17) and a cis-blocked Pd(II) precursor [cis-(en)Pd(NO₃)₂] (Bhat et al., 2017). The affinity of the barrel's cavity to hydrophobic guest molecules has been employed in encapsulation of the anticancer drug curcumin, which resulted in significant stabilization of curcumin and its solubilization in water. This reflected on the increased anticancer activity of water solutions of curcumin against HeLa cancer cells demonstrating the successful utility of the metallosupramolecular barrel as a carrier and protector for photosensitive and hydrophobic drugs. Although stability and cytotoxicity of the empty barrels alone have not been investigated, this study clearly demonstrated the increased bioavailability and stability of curcumin in water solutions as seen from the fluorescence microscopy imaging of the cancer cells and the increased cytotoxicity with respect to the curcumin concentration.

A similar tetrafacial barrel with composition Pd₈L₄ (29, Figure 10) has been built from tetraimidazolyl carbazole ligand (L18) (Roy et al., 2015). This metallosupramolecule has a tubular morphology that was fully characterized by multinuclear 1D/2D NMR and ESI-MS as well as by single-crystal X-ray diffraction of the coronene enclosing host-guest complex. While the barrel itself is water soluble, its hydrophobic cavity renders it ideal to solubilize hydrophobic guest molecules. Moreover, the inclusion of polyaromatic hydrocarbons with strong optical absorption and emission properties is accompanied with color and/or emission change. This property has been employed in live-cell fluorescence microscopy imaging by the water soluble perylene enclosing barrel, which demonstrated enhanced cell membrane permeability of the inclusion complex. This example gives the hope for further successful utilization of water soluble metalosupramolecules as drug or probe carriers provided their biocompatibility, stability and drug release properties is proved.

The flexible tetra(pyridin-4-yl)benzene-1,4-amine (L17, Figure 10) has also been used to construct organometallic octanuclear Ru(II) cages from dinuclear p-cymene ruthenium(II) acceptors, used as bridging oxo-pillars that vary from oxalate, benzoquinones, naphthoquinones (Ajibola Adeyemo et al., 2017). The largest box was characterized by X-ray diffraction besides the NMR, ESI-MS and electronic absorption spectra that have been used for characterization of all other boxes. All Ru8 cages showed strong anticancer activity in vitro that is comparable with cytotoxicity of cisplatin against A549 and HeLa cells. The anticancer profiles showed increasing cytotoxicity by enlarging the aromatic unit of the oxo-bridging pillars.

Conclusions

From the presented recent examples for the fast-expanding chemotherapeutic applications of metallosupramolecular entities, it can be concluded that this class of compounds provide a rich field for exploration. The diversity in their properties stems from the fact that the metallosupramolecules can intelligently combine metal hinges of different coordination and reactivity properties as well as multidentate ligands that can be readily decorated without affecting the self-assembly processes. This has presented several working strategies to either increase the size or stability of the formed metalosupramolecular structures or to link them with receptor specific vectors. Construction of larger size composites that incorporate metallosupramolecules proved useful to target and deliver both therapeutic and imaging probes. The unique property of the 3D structures of metallacages, capsules, and barrels, is their discrete cavity which opens up further prospects for biological applications. Among the mostly explored ones are the encapsulation and delivery of bioactive molecules. Thereby, one and the same metallosupramolecular host can be used for different biological (therapeutic or diagnostic) purposes depending on the properties of the encapsulated guest molecule. The demonstrated encapsulation of Pt(IV)-prodrugs, which can be activated and subsequently released in the intracellular media by selective reduction and formation of the corresponding Pt(II)-drug, is an intriguing advancement to fully explore the potential for biomedical applications of the coordination supramolecules. Moreover, the encapsulation of a fluorescein-bearing Pt(IV)-prodrugs by the Pt-cage 1, shown in Figure 9, had actually added imaging modality to the selective anticancer treatment. The presented examples are only some of the suggestions on how to augment the cancer treatment potential of such host-guest complexes with additional imaging modality.

The showed examples can expand in the near future toward modifying or multiplying the main therapeutic action by introducing immunomodulatory, anti- or pro-oxidative molecules, or other bioactive fragments either as guest molecules or as exo-functionalization of the supramolecular hosts. The proof-of-concept for exo-functionalization of metallosupramolecules with large peptides has already been demonstrated and is expected to open additional prospect to construct hybrid systems with larger size (>30 nm) that can benefit from both the targeting properties of the attached biomolecule and the truly efficient EPR effect.

The flexibility to modulate the overall stability of the 3D metallosupramolecular structures, by simply encapsulating appropriate guest molecule is another approach to fine-tune either the delivery properties or their therapeutic potential. In the cases when the increased stability affects the biological action of the supramolecules, guest-encapsulation can be exploited to turn the toxic species into non-toxic or vice versa, and thus, transform them from toxic anticancer agents to safe drug-delivery vehicles. Exploiting also building components with interesting optical or redox properties enriches further the possibilities to adjust the structure and properties of the constructed metallosupramolecules to the desired biomedical application. Inevitably, the presented examples are an expanding source of inspiration for many synthetic and biological chemists to, hopefully, allow them fully reveal the multifaceted potential of the metallosupramolecules.

Author Contributions

The author confirms being the sole contributor of this work and has approved it for publication.

Conflict of Interest Statement

The author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

The National Science Fund of Bulgaria is gratefully acknowledged for the financial support (DFNI-B02/24). The support from H2020-Twinning project (Materials Networking; 692146) is gratefully acknowledged.

Abbreviations

CDDP, cisplatin; SISO, cervix adenocarcinoma; A-427, lung carcinoma; LCLC-103H, large cell lung cancer; 5637, urinary bladder carcinoma; U2OS, osteosarcoma; VM-1, cisplatin resistant melanoma cell line; MCF7, breast cancer; A549, lung carcinoma; KB, oral cancer; HaCaT, skin keratinocyte; MDA-MB-231, human breast adenocarcinoma cell line; T98G, (brain tumor); SNU-80, (thyroid cancer); HEK-293, human embryonic kidney cells (non-malignant cell lines); HepG2, human hepatocellular carcinoma; DU-145, prostate cancer cells; MCF10A, immortalized non-malignant breast tissue; SKOV-3, Human ovarian cancer; HBL100 and T47D, Human breast-tumor cell lines; HT-29, colorectal adenocarcinoma cells; T-24, bladder cancer cells; SKW-3, T-cell leukemia; HL-60, acute myelocyte leukemia; HL-60/Dox and HL-60/CDDP, Doxorubicin and cisplatin-resistant counterparts of the HL-60 cells.

References

Ahmedova, A., Mihaylova, R., Momekova, D., Shestakova, P., Stoykova, S., Zaharieva, J., et al. (2016a). M2L4 coordination capsules with tunable anticancer activity upon guest encapsulation. Dalton Trans. 45, 13214–13221. doi: 10.1039/C6DT01801G

PubMed Abstract | CrossRef Full Text | Google Scholar

Ahmedova, A., Momekova, D., Yamashina, M., Shestakova, P., Momekov, G., Akita, M., et al. (2016b). Anticancer Potencies of Pt(II) - and Pd(II)-linked M2L4 coordination capsules with improved selectivity. Chem. Asian J. 11, 474–477. doi: 10.1002/asia.201501238

PubMed Abstract | CrossRef Full Text | Google Scholar

Aird, R. E., Cummings, J., Ritchie, A. A., Muir, M., Morris, R. E., Chen, H., et al. (2002). In vitro and in vivo activity and cross resistance profiles of novel ruthenium (II) organometallic arene complexes in human ovarian cancer. Br. J. Cancer 86, 1652–1657. doi: 10.1038/sj.bjc.6600290

PubMed Abstract | CrossRef Full Text | Google Scholar

Ajibola Adeyemo, A., Shettar, A., Bhat, I. A., Kondaiah, P., and Mukherjee, P. S. (2017). Self-Assembly of discrete RuII8 molecular cages and their in vitro anticancer activity. Inorg. Chem. 56, 608–617. doi: 10.1021/acs.inorgchem.6b02488

PubMed Abstract | CrossRef Full Text | Google Scholar

Allison, S. J., Cooke, D., Davidson, F. S., Elliott, P. I. P., Faulkner, R. A., Griffiths, H. B. S., et al. (2018). Ruthenium-containing linear helicates and mesocates with tuneable p53-selective cytotoxicity in colorectal cancer cells. Angew. Chem. 130, 9947–9952. doi: 10.1002/ange.201805510

CrossRef Full Text | Google Scholar

Bhat, I. A., Jain, R., Siddiqui, M. M., Saini, D. K., and Mukherjee, P. S. (2017). Water-soluble Pd8L4 self-assembled molecular barrel as an aqueous carrier for hydrophobic curcumin. Inorg. Chem. 56, 5352–5360. doi: 10.1021/acs.inorgchem.7b00449

PubMed Abstract | CrossRef Full Text | Google Scholar

Bhowmick, S., Jana, A., Singh, K., Gupta, P., Gangrade, A., Mandal, B. B., et al. (2018). Coordination-driven self-assembly of ionic irregular hexagonal metallamacrocycles via an organometallic clip and their cytotoxicity potency. Inorg. Chem. 57, 3615–3625. doi: 10.1021/acs.inorgchem.7b01561

PubMed Abstract | CrossRef Full Text | Google Scholar

Bilbeisi, R. A., Olsen, J. C., Charbonnière, L. J., and Trabolsi, A. (2014). Self-assembled discrete metal-organic complexes: Recent advances. Inorg Chim Acta 417, 79–108. doi: 10.1016/j.ica.2013.12.015

CrossRef Full Text | Google Scholar

Casini, A., Woods, B., and Wenzel, M. (2017). The promise of self-assembled 3D supramolecular coordination complexes for biomedical applications. Inorg. Chem. 56, 14715–14729. doi: 10.1021/acs.inorgchem.7b02599

PubMed Abstract | CrossRef Full Text | Google Scholar

Chen, L. J., Chen, S., Qin, Y., Xu, L., Yin, G. Q., Zhu, J. L., et al. (2018). Construction of porphyrin-containing metallacycle with improved stability and activity within mesoporous carbon. J. Am. Chem. Soc. 140, 5049–5052. doi: 10.1021/jacs.8b02386

PubMed Abstract | CrossRef Full Text | Google Scholar

Cook, T. R., and Stang, P. J. (2015). Recent developments in the preparation and chemistry of metallacycles and metallacages via coordination. Chem. Rev. 115, 7001–7045. doi: 10.1021/cr5005666

PubMed Abstract | CrossRef Full Text | Google Scholar

Cook, T. R., Vajpayee, V., Lee, M. H., Stang, P. J., and Chi, K. W. (2013). Biomedical and biochemical applications of self-assembled metallacycles and metallacages. Acc. Chem. Res. 46, 2464–2474. doi: 10.1021/ar400010v

PubMed Abstract | CrossRef Full Text | Google Scholar

Crowley, J. D., and Gavey, E. L. (2010). Use of di-1,4-substituted-1,2,3-triazole “click” ligands to self-assemble dipalladium(ii) coordinatively saturated, quadruply stranded helicate cages. Dalton Trans. 39, 4035–4037. doi: 10.1039/C003683H

PubMed Abstract | CrossRef Full Text | Google Scholar

Datta, S., Saha, M. L., and Stang, P. J. (2018). Hierarchical assemblies of supramolecular coordination complexes. Acc. Chem. Res. 51, 2047–2063. doi: 10.1021/acs.accounts.8b00233

PubMed Abstract | CrossRef Full Text | Google Scholar

Domarco, O., Lötsch, D., Schreiber, J., Dinhof, C., Van Schoonhoven, S., García, M. D., et al. (2017). Self-assembled Pt2L2 boxes strongly bind G-quadruplex DNA and influence gene expression in cancer cells. Dalton Trans. 46, 329–332. doi: 10.1039/c6dt03876j

PubMed Abstract | CrossRef Full Text | Google Scholar

Dubey, A., Min, J. W., Koo, H. J., Kim, H., Cook, T. R., Kang, S. C., et al. (2013). Anticancer potency and multidrug-resistant studies of self-assembled arene-ruthenium metallarectangles. Chem. Eur. J. 19, 11622–11628. doi: 10.1002/chem.201300870

PubMed Abstract | CrossRef Full Text | Google Scholar

Grishagin, I. V., Pollock, J. B., Kushal, S., Cook, T. R., Stang, P. J., and Olenyuk, B. Z. (2014). In vivo anticancer activity of rhomboidal Pt(II) metallacycles. Proc. Natl. Acad. Sci. U.S.A.111, 18448–18453. doi: 10.1073/pnas.1418712111

PubMed Abstract | CrossRef Full Text | Google Scholar

Gupta, G., Das, A., Park, K. C., Tron, A., Kim, H., Mun, J., et al. (2017). Self-Assembled Novel BODIPY-based palladium supramolecules and their cellular localization. Inorg. Chem. 56, 4616–4622. doi: 10.1021/acs.inorgchem.7b00260

PubMed Abstract | CrossRef Full Text | Google Scholar

Han, J., Schmidt, A., Zhang, T., Permentier, H., Groothuis, G. M., Bischoff, R., et al. (2017). Bioconjugation strategies to couple supramolecular exo-functionalized palladium cages to peptides for biomedical applications. Chem. Commun. 53, 1405–1408. doi: 10.1039/c6cc08937b

PubMed Abstract | CrossRef Full Text | Google Scholar

Hotze, A. C., Kariuki, B. M., and Hannon, M. J. (2006). Dinuclear double-stranded metallosupramolecular ruthenium complexes: potential anticancer drugs. Angew Chem. 45, 4839–4842. doi: 10.1002/anie.200601351

PubMed Abstract | CrossRef Full Text | Google Scholar

Ibukuro, F., Kusukawa, T., and Fujita, M. (1998). A thermally switchable molecular lock. Guest-templated synthesis of a kinetically stable nanosized cage. J. Am.Chem. Soc. 120, 8561–8562. doi: 10.1021/ja980853f

CrossRef Full Text

Jana, A., Bhowmick, S., Kumar, S., Singh, K., Garg, P., and Das, N. (2019). Self-assembly of Pt(II) based nanoscalar ionic hexagons and their anticancer potencies. Inorg. Chim. Acta 484, 19–26. doi: 10.1016/j.ica.2018.09.009

CrossRef Full Text | Google Scholar

Jana, A., Lippmann, P., Ott, I., and Das, N. (2018). Self-assembly of flexible [2 + 2] ionic metallamacrocycles and their cytotoxicity potency. Inorg. Chim Acta 471, 223–227. doi: 10.1016/j.ica.2017.11.002

CrossRef Full Text | Google Scholar

Kaiser, F., Schmidt, A., Heydenreuter, W., Altmann, P. J., Casini, A., Sieber, S. A., et al. (2016). Self-assembled palladium and platinum coordination cages: photophysical studies and anticancer activity. Eur. J. Inorg. Chem. 2016, 5189–5196. doi: 10.1002/ejic.201600811

CrossRef Full Text | Google Scholar

Kaner, R. A., Allison, S. J., Faulkner, A. D., Phillips, R. M., Roper, D. I., Shepherd, S. L., et al. (2016). Anticancer metallohelices: nanomolar potency and high selectivity. Chem. Sci. 7, 951–958. doi: 10.1039/c5sc03677a

PubMed Abstract | CrossRef Full Text | Google Scholar

Kishi, N., Li, Z., Yoza, K., Akita, M., and Yoshizawa, M. (2011). An M2L4 molecular capsule with an anthracene shell: encapsulation of large guests up to 1nm. J. Am. Chem. Soc. 133, 11438–11441. doi: 10.1021/ja2037029

CrossRef Full Text | Google Scholar

Leung, C. H., Kang, T. S., Yang, C., Leung, K. H., and Ma, D. L. (2017). “Biological applications of supramolecular coordination complexes,” in Comprehensive Supramolecular Chemistry II, ed J. L. Atwood (Elsevier Inc.), 3–20.

Lewis, J. E., McAdam, C. J., Gardiner, M. G., and Crowley, J. D. (2013). A facile “click” approach to functionalised metallosupramolecular architectures. Chem. Commun. 49, 3398–3400. doi: 10.1039/c3cc41209a

PubMed Abstract | CrossRef Full Text | Google Scholar

Lewis, J. E. M., Elliott, A. B. S., McAdam, C. J., Gordon, K. C., and Crowley, J. D. (2014). ‘Click' to functionalise: synthesis, characterisation and enhancement of the physical properties of a series of exo- and endo-functionalised Pd2L4 nanocages. Chem. Sci. 5, 1833–1843. doi: 10.1039/c4sc00434e

CrossRef Full Text | Google Scholar

Lewis, J. E. M., Gavey, E. L., Cameron, S. A., and Crowley, J. D. (2012). Stimuli-responsive Pd2L4metallosupramolecular cages: towards targeted cisplatin drug delivery. Chem. Sci. 3, 778–784. doi: 10.1039/c2sc00899h

CrossRef Full Text | Google Scholar

Li, M., Howson, S. E., Dong, K., Gao, N., Ren, J., Scott, P., et al. (2014). Chiral metallohelical complexes enantioselectively target amyloid beta for treating Alzheimer's disease. J. Am. Chem. Soc. 136, 11655–11663. doi: 10.1021/ja502789e

PubMed Abstract | CrossRef Full Text | Google Scholar

Li, Z., Kishi, N., Yoza, K., Akita, M., and Yoshizawa, M. (2012). Isostructural M2L4 molecular capsules with anthracene shells: Synthesis, crystal structures, and fluorescent properties. Chem - Eur J 18, 8358–8365. doi: 10.1002/chem.201200155

PubMed Abstract | CrossRef Full Text | Google Scholar

Ma, X., and Zhao, Y. (2015). Biomedical applications of supramolecular systems based on host-guest interactions. Chem. Rev. 115, 7794–7839. doi: 10.1021/cr500392w

PubMed Abstract | CrossRef Full Text | Google Scholar

Malina, J., Hannon, M. J., and Brabec, V. (2008). DNA binding of dinuclear iron(II) metallosupramolecular cylinders. DNA unwinding and sequence preference. Nucleic Acids Res. 36, 3630–3638. doi: 10.1093/nar/gkn244

PubMed Abstract | CrossRef Full Text | Google Scholar

Malina, J., Hannon, M. J., and Brabec, V. (2014). Recognition of DNA bulges by dinuclear iron(II) metallosupramolecular helicates. FEBS J. 281, 987–997. doi: 10.1111/febs.12696

PubMed Abstract | CrossRef Full Text | Google Scholar

Malina, J., Hannon, M. J., and Brabec, V. (2016). Iron(II) supramolecular helicates interfere with the HIV-1 Tat-TAR RNA interaction critical for viral replication. Sci. Rep. 6:29674. doi: 10.1038/srep29674

PubMed Abstract | CrossRef Full Text | Google Scholar

Mattsson, J., Govindaswamy, P., Renfrew, A. K., Dyson, P. J., Stepnicka, P., Süss-Fink, G., et al. (2009). Synthesis, molecular structure and anticancer activity of cationic arene ruthenium metallarectangles. Organometallics 28, 4350–4357. doi: 10.1021/om900359j

CrossRef Full Text | Google Scholar

McConnell, A. J., Wood, C. S., Neelakandan, P. P., and Nitschke, J. R. (2015). Stimuli-responsive metal-ligand assemblies. Chem. Rev. 115, 7729–7793. doi: 10.1021/cr500632f

PubMed Abstract | CrossRef Full Text | Google Scholar

McNeill, S. M., Preston, D., Lewis, J. E., Robert, A., Knerr-Rupp, K., Graham, D. O., et al. (2015). Biologically active [Pd2L4](4+) quadruply-stranded helicates: stability and cytotoxicity. Dalton Trans. 44, 11129–11136. doi: 10.1039/c5dt01259g

PubMed Abstract | CrossRef Full Text | Google Scholar

Mishra, A., Lee, S. C., Kaushik, N., Cook, T. R., Choi, E. H., Kaushik, N. K., et al. (2014). Self-assembled supramolecular hetero-bimetallacycles for anticancer potency by intracellular release. Chem. Eur. J. 20, 14410–14420. doi: 10.1002/chem.201403372

PubMed Abstract | CrossRef Full Text | Google Scholar

Mounir, M., Lorenzo, J., Ferrer, M., Prieto, M. J., Rossell, O., Aviles, F. X., et al. (2007). DNA interaction and antiproliferative behavior of the water soluble platinum supramolecular squares [(en)Pt(N-N)]4(NO3)8 (en = ethylenediamine, N-N = 4,4'-bipyridine or 1,4-bis(4-pyridyl)tetrafluorobenzene). J. Inorg. Biochem. 101, 660–666. doi: 10.1016/j.jinorgbio.2006.12.009

PubMed Abstract | CrossRef Full Text | Google Scholar

Pascu, G. I., Hotze, A. C., Sanchez-Cano, C., Kariuki, B. M., and Hannon, M. J. (2007). Dinuclear ruthenium(II) triple-stranded helicates: luminescent supramolecular cylinders that bind and coil DNA and exhibit activity against cancer cell lines. Angew Chem. 46, 4374–4378. doi: 10.1002/anie.200700656

PubMed Abstract | CrossRef Full Text | Google Scholar

Preston, D., McNeill, S. M., Lewis, J. E., Giles, G. I., and Crowley, J. D. (2016). Enhanced kinetic stability of [Pd2L4](4+) cages through ligand substitution. Dalton Trans. 45, 8050–8060. doi: 10.1039/c6dt00133e

PubMed Abstract | CrossRef Full Text | Google Scholar

Rodriguez, J., Mosquera, J., Couceiro, J. R., Nitschke, J. R., Vazquez, M. E., and Mascarenas, J. L. (2017). Anion recognition as a supramolecular switch of cell internalization. J. Am. Chem. Soc. 139, 55–58. doi: 10.1021/jacs.6b11103

PubMed Abstract | CrossRef Full Text | Google Scholar

Roy, B., Ghosh, A. K., Srivastava, S., D'Silva, P., and Mukherjee, P. S. (2015). A Pd8 tetrafacial molecular barrel as carrier for water insoluble fluorophore. J. Am. Chem. Soc. 137, 11916–11919. doi: 10.1021/jacs.5b08008

PubMed Abstract | CrossRef Full Text | Google Scholar

Schmidt, A., Casini, A., and Kühn, F. E. (2014). Self-assembled M2L4 coordination cages: synthesis and potential applications. Coord. Chem. Rev. 275 19–36. doi: 10.1016/j.ccr.2014.03.037

CrossRef Full Text | Google Scholar

Schmidt, A., Hollering, M., Drees, M., Casini, A., and Kühn, F. E. (2016a). Supramolecular: Exo -functionalized palladium cages: Fluorescent properties and biological activity. Dalton Trans. 45, 8556–8565. doi: 10.1039/c6dt00654j

PubMed Abstract | CrossRef Full Text | Google Scholar

Schmidt, A., Molano, V., Hollering, M., Poethig, A., Casini, A., and Kühn, F. E. (2016b). Evaluation of new palladium cages as potential delivery systems for the anticancer drug cisplatin. Chem. Eur. J. 22, 2253–2256. doi: 10.1002/chem.201504930

PubMed Abstract | CrossRef Full Text | Google Scholar

Schmitt, F., Freudenreich, J., Barry, N. P. E., Juillerat-Jeanneret, L., Süss-Fink, G., and Therrien, B. (2012). Organometallic cages as vehicles for intracellular release of photosensitizers. J. Am. Chem. Soc. 134, 754–757. doi: 10.1021/ja207784t

PubMed Abstract | CrossRef Full Text | Google Scholar

Smith, G. S., and Therrien, B. (2011). Targeted and multifunctional arene ruthenium chemotherapeutics. Dalton Trans. 40, 10793–10800. doi: 10.1039/c1dt11007a

PubMed Abstract | CrossRef Full Text | Google Scholar

Terenzi, A., Ducani, C., Blanco, V., Zerzankova, L., Westendorf, A. F., Peinador, C., et al. (2012). DNA binding studies and cytotoxicity of a dinuclear PtII diazapyrenium-based metallo-supramolecular rectangular box. Chem. Eur. J. 18, 10983–10990. doi: 10.1002/chem.201201519

PubMed Abstract | CrossRef Full Text | Google Scholar

Therrien, B. (2013). Transporting and shielding photosensitisers by using water-soluble organometallic cages: a new strategy in drug delivery and photodynamic therapy. Chem. Eur. J. 19, 8378–8386. doi: 10.1002/chem.201301348

PubMed Abstract | CrossRef Full Text | Google Scholar

Therrien, B., Süss-Fink, G., Govindaswamy, P., Renfrew, A. K., and Dyson, P. J. (2008). The “complex-in-a-complex” cations [(acac)2M-Ru6-(p-iPrC6H4Me)6(tpt) 2(dhbq)3]6+: A trojan horse for cancer cells. Angew. Chem. Int. Ed Engl. 47, 3773–3776. doi: 10.1002/anie.200800186

PubMed Abstract | CrossRef Full Text | Google Scholar

Tian, Y., Yan, X., Saha, M. L., Niu, Z., and Stang, P. J. (2016). Hierarchical Self-Assembly of Responsive Organoplatinum(II) Metallacycle-TMV Complexes with Turn-On Fluorescence. J. Am. Chem. Soc. 138, 12033–12036. doi: 10.1021/jacs.6b07402

PubMed Abstract | CrossRef Full Text | Google Scholar

Vajpayee, V., Lee, S. M., Park, J. W., Dubey, A., Kim, H., Cook, T. R., et al. (2013). Growth inhibitory activity of a bis-benzimidazole-bridged arene ruthenium metalla-rectangle and prism. Organometallics 32, 1563–1566. doi: 10.1021/om301174s

PubMed Abstract | CrossRef Full Text | Google Scholar

Vellas, S. K., Lewis, J. E., Shankar, M., Sagatova, A., Tyndall, J. D., Monk, B. C., et al. (2013). [Fe(2)L(3)](4)(+) cylinders derived from bis(bidentate) 2-pyridyl-1,2,3-triazole “click” ligands: synthesis, structures and exploration of biological activity. Molecules 18, 6383–6407. doi: 10.3390/molecules18066383

CrossRef Full Text | Google Scholar

Yamashina, M., Akita, M., Hasegawa, T., Hayashi, S., and Yoshizawa, M. (2017). A polyaromatic nanocapsule as a sucrose receptor in water. Sci. Adv. 3:e1701126. doi: 10.1126/sciadv.1701126

PubMed Abstract | CrossRef Full Text | Google Scholar

Yamashina, M., Matsuno, S., Sei, Y., Akita, M., and Yoshizawa, M. (2016). Recognition of multiple methyl groups on aromatic rings by a polyaromatic cavity. Chem. Eur. J. 22, 14147–14150. doi: 10.1002/chem.201603032

PubMed Abstract | CrossRef Full Text | Google Scholar

Yamashina, M., Sei, Y., Akita, M., and Yoshizawa, M. (2014). Safe storage of radical initiators within a polyaromatic nanocapsule. Nature Commun. 5:4662. doi: 10.1038/ncomms5662

PubMed Abstract | CrossRef Full Text | Google Scholar

Yan, H., Süss-Fink, G., Neels, A., and Stoeckli-Evans, H. (1997). Mono-, di- and tetra-nuclear p-cymeneruthenium complexes containing oxalato ligands. J. Chem. Soc. Dalton Trans. 4345–4350. doi: 10.1039/a704658h

CrossRef Full Text | Google Scholar

Yan, X., Wang, M., Cook, T. R., Zhang, M., Saha, M. L., Zhou, Z., et al. (2016). Light-emitting superstructures with anion effect: coordination-driven self-assembly of pure tetraphenylethylene metallacycles and metallacages. J. Am. Chem. Soc. 138, 4580–4588. doi: 10.1021/jacs.6b00846

PubMed Abstract | CrossRef Full Text | Google Scholar

Yoshizawa, M., Klosterman, J. K., and Fujita, M. (2009). Functional molecular flasks: new properties and reactions within discrete, self-assembled hosts. Angew. Chem. Int Ed 48, 3418–3438. doi: 10.1002/anie.200805340

PubMed Abstract | CrossRef Full Text | Google Scholar

Yu, G., Cook, T. R., Li, Y., Yan, X., Wu, D., Shao, L., et al. (2016). Tetraphenylethene-based highly emissive metallacage as a component of theranostic supramolecular nanoparticles. Proc. Natl. Acad. Sci. U.S.A. 113, 13720–13725. doi: 10.1073/pnas.1616836113

PubMed Abstract | CrossRef Full Text | Google Scholar

Yue, Z., Wang, H., Bowers, D. J., Gao, M., Stilgenbauer, M., Nielsen, F., et al. (2018). Nanoparticles of metal-organic cages designed to encapsulate platinum-based anticancer agents. Dalton Trans. 47, 670–674. doi: 10.1039/c7dt03537c

PubMed Abstract | CrossRef Full Text | Google Scholar

Zarra, S., Wood, D. M., Roberts, D. A., and Nitschke, J. R. (2015). Molecular containers in complex chemical systems. Chem. Soc. Rev. 44, 419–432. doi: 10.1039/C4CS00165F

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhang, M., Li, S., Yan, X., Zhou, Z., Saha, M. L., Wang, Y. C., et al. (2016). Fluorescent metallacycle-cored polymers via covalent linkage and their use as contrast agents for cell imaging. Proc. Natl. Acad. Sci. U.S.A. 113, 11100–11105. doi: 10.1073/pnas.1612898113

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhang, Y.-Y., Gao, W.-X., Lin, L., and Jin, G.-X. (2017). Recent advances in the construction and applications of heterometallic macrocycles and cages. Coord. Chem. Rev. 344, 323–344. doi: 10.1016/j.ccr.2016.09.010

CrossRef Full Text | Google Scholar

Zheng, Y. R., Suntharalingam, K., Bruno, P. M., Lin, W., Wang, W., Hemann, M. T., et al. (2016). Mechanistic Studies of the Anticancer Activity of An Octahedral Hexanuclear Pt(II) Cage. Inorg. Chim. Acta 452, 125–129. doi: 10.1016/j.ica.2016.03.021

PubMed Abstract | CrossRef Full Text | Google Scholar

Zheng, Y. R., Suntharalingam, K., Johnstone, T. C., and Lippard, S. J. (2015). Encapsulation of Pt(IV) Prodrugs within a Pt(II) cage for drug delivery. Chem. Sci. 6, 1189–1193. doi: 10.1039/C4SC01892C

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhou, J., Zhang, Y., Yu, G., Crawley, M. R., Fulong, C. R. P., Friedman, A. E., et al. (2018a). Highly Emissive Self-Assembled BODIPY-Platinum Supramolecular Triangles. J. Am. Chem. Soc. 140, 7730–7736. doi: 10.1021/jacs.8b04929

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhou, Z., Liu, J., Rees, T. W., Wang, H., Li, X., Chao, H., et al. (2018b). Heterometallic Ru-Pt metallacycle for two-photon photodynamic therapy. Proc. Natl. Acad. Sci. U.S.A. 115, 5664–5669. doi: 10.1073/pnas.1802012115

PubMed Abstract | CrossRef Full Text | Google Scholar