In the original article, there was a mistake in Table 1: Protocols tested for assessing inactivation using lysis buffers as published. During the publication process the components for each of the three kits tested in this study (as stated in the ‘Reagents’ and ‘Active virucidal components’ columns), were unclearly formatted. The corrected Table 1: Protocols tested for assessing inactivation using lysis buffers appears below.
Table 1
| Manufacturer, RNA extraction kit, Platform | Reagents (volume/sample) | Active virucidal components* | Reagent: Sample ratio |
|---|---|---|---|
| Qiagen, QIAamp 96 Virus QIAcube HT Kit (Cat #: 57731), Qiagen Qiacube HT. (Referred to here as Qiagen protocol) | ACL buffer (190 µl) | GITC 30 - <50% | 1.6: 1 |
| ATL buffer (100 µl) | 1 - <3% SDS | ||
| Proteinase K (20 µl) | |||
| Carrier RNA (5 µl) | |||
| MS2 (10 µl) | |||
| ThermoFisher, MagMax Pathogen RNA/DNA kit (Cat #: 4462359), Kingfisher Flex. (Referred to here as MagMax Protocol 1) | Lysis binding buffer (350 µl) | GITC 55-80% <0.001% Acrylamide Zwittergent | 3.8: 1 |
| Isopropanol (300 µl) | 100% 2-propanol | ||
| Carrier RNA (2 µl) | |||
| Water (100 µl) | |||
| MS2 (10 µl) | |||
| ThermoFisher, MagMax viral/pathogen nucleic acid isolation kit (Cat #: A48310), Kingfisher Flex. (Referred to here as MagMax Protocol 2) | Lysis binding buffer (265 µl) | GITC 55-80% <0.001% Acrylamide Zwittergent | 1.4: 1 |
| Proteinase K (5 µl) | |||
| †Water (Magnetic beads) (10 µl) | |||
| MS2 (10 µl) |
Protocols tested for assessing inactivation using lysis buffers.
*As identified directly from components, manufacturer information, or inferred from the associated MSDS.
†Water was used to replace the magnetic beads as the washing steps described below would not remove the beads and the beads interfered the read-out of the TCID-50 assay.
GITC, Guanidinium thiocyanate; SDS, Sodium dodecyl sulphate.
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
Publisher’s Note
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Summary
Keywords
SARS-CoV-2, high throughput, PCR, biosafety, laboratory-acquired infection, clinical diagnosis
Citation
Thom RE, Eastaugh LS, O’Brien LM, Ulaeto DO, Findlay JS, Smither SJ, Phelps AL, Stapleton HL, Hamblin KA and Weller SA (2021) Corrigendum: Evaluation of the SARS-CoV-2 Inactivation Efficacy Associated With Buffers From Three Kits Used on High-Throughput RNA Extraction Platforms. Front. Cell. Infect. Microbiol. 11:813442. doi: 10.3389/fcimb.2021.813442
Received
11 November 2021
Accepted
23 November 2021
Published
08 December 2021
Volume
11 - 2021
Edited and reviewed by
Max Maurin, Université Grenoble Alpes, France
Updates
Copyright
© 2021 Thom, Eastaugh, O’Brien, Ulaeto, Findlay, Smither, Phelps, Stapleton, Hamblin and Weller.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Simon A. Weller, sweller@dstl.gov.uk
This article was submitted to Clinical Microbiology, a section of the journal Frontiers in Cellular and Infection Microbiology
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.