Soil Application of Wheat Straw Vermicompost Enhances Morpho-Physiological Attributes and Antioxidant Defense in Wheat Under Drought Stress

Introduction

Wheat (Triticum aestivum L.) is a significant crop that is cultivated throughout the world. The crop is cultivated on an area of 214.79 million hectares with a total production of 735.17 million tons on the globe (FAO, 2019). It is a dominant cereal crop that provides a food source for over 1.2 billion people and is of significant importance as a commercial product (Iqbal et al., 2018). Wheat is a C3 crop and is particularly vulnerable to harsh environments, including drought stress (Wang et al., 2014), which affects the growth and developmental processes, particularly when it occurs at early developmental stages. Drought stress also disrupts the physiological events in crop plants as it reduces chlorophyll content (Ahmad et al., 2022), photosynthetic assimilation of CO₂ (Golldack et al., 2011; Wei et al., 2015), inhibits cell division and elongation (Avramova et al., 2015), and decreases photosynthesis-related parameters and relative water contents (Hussain et al., 2019; Ahmad et al., 2021a). Inevitably, prolonged drought also results in oxidative damage owing to increasing the production of reactive oxygen species (ROS) that markedly affected cell membranes and caused tissue injuries (Reddy et al., 2004; Liu et al., 2015; Farooq et al., 2020). Nonetheless, different enzymatic and non-enzymatic antioxidants, also called scavenging enzymes (including ascorbic acid, superoxide dismutase, peroxidase, and catalase), are activated in plants as a defense response to detoxify the ROS (Abid et al., 2016; Hussain et al., 2018). Prolonged drought episodes also caused a significant reduction in grain yield which accounted for a 20–40% reduction in wheat yield globally (Daryanto et al., 2016). In recent years, various approaches are being used to minimize the negative effects of drought; these include the application of osmoprotectants, cultivation of drought-tolerant cultivars, and the conservation of agricultural practices using conservation tillage, natural and synthetic mulches, and vermicompost application.

In today’s agriculture scenario, the main concern of researchers and farmers is to enhance crop productivity in a sustainable way to maintain soil fertility and protect the environment. Hence, vermicompost application has great potential to promote plant growth and grain yield while protecting the soil environment (Varghese and Prabha, 2014). Its application enhances the physiochemical and organic properties of the soil. Vermicompost, degraded solid organic residues formed under aerobic conditions, contains a bulk quantity of earthworms and other microfauna that enhance the availability of growth regulators/hormones, degrading enzymes (including chitinase, cellulase, lipase, amylase, and proteases), and vitamins. These earthworms also help in the modification and fragmentation of organic residues by lowering the C/N ratio and exposed area for microfauna to react with cellulolytic-degrading microfauna for complete degradation. Earthworm guts can release bacterial excrements which are the product called humus (Dominguez and Edwars, 2004). The epigeic earthworms, Eisenia fetida and E. andrei, are among the most commonly used worm types in vermiculture; however, some other species including Polipheretima elongate, Millsonia anomala, and Pontoscolex corethurus are also used in the preparation of vermicompost (Lattaud et al., 1997a, b; Urbasek and Pizl 1991; Zhang et al., 1993; Zhang et al., 2000).

Vermicompost application plays a pivotal role in regulating drought tolerance in crop plants. In this regard, Curtis and Classsen (2005) demonstrated that applying vermicompost significantly enhanced the availability of soil water, increased leaf water content, and enhanced plant biomass production. Working with maize and soybean crops, Bowden et al. (2010) confirmed that vermicompost application enhanced drought tolerance as it promoted the antioxidant enzyme activities and photosynthesis traits. According to Suthar (2009), the application of vermicompost improves the drought tolerance of garlic (Allium sativum L.) grown under pot conditions. The positive influence of vermicompost was also reported for other field-grown crops, such as Triticum aestivum L. (wheat), Capsicum annum L. (peppers), Brassica oleracea L. (cabbage), and Lycopersicum esculentom L. (tomatoes) (reviewed by Subler et al., 1998). Working with chickpea (Cicer arietinum L.) crop, Jat and Ahlawat (2006) reported that vermicompost improved the soil nutrient status, thereby promoting crop growth.

The positive influence of vermicompost application combined with other organic fertilizers on morpho-physiological and yield traits under drought stress has been well-discussed in previous studies (Aboelsoud and Ahmed, 2020; Hafez et al., 2020). The vermicompost fertilizer and humic acid application have been reported to induce the activities of scavenging enzymes, including antioxidant enzyme catalase, superoxide dismutase, and peroxidase, to detoxify the ROS (Garcia et al., 2012; Kiran, 2019). A similar phenomenon has also been reported in chickpea (Gholipoor et al., 2011; Hosseinzadeh et al., 2018), tomato (Solanum lycopersicon L.) (Ahanger et al., 2021), mungbean (Mahmoudi et al., 2016), lentil (Ahmadpour and Hosseinzadeh, 2017), and the oilseed crop: canola (Rashtbari et al., 2012). Considering the aforementioned scenario, the present investigation was focused on studying the effect of wheat straw vermicompost application on wheat morphology and physiology under water stress and investigating the damaging effects of drought stress on wheat growth. In the current study, we hypothesized that wheat straw vermicompost will improve wheat growth and drought tolerance by modulating the physiological and biochemical characteristics of our crop.

Materials and Methods

Experimental Station and Plant Material

The concurrent study was executed as a pot experiment in the wirehouse at the Student Research Farm, Department of Agronomy, Faculty of Agriculture, University of Agriculture, Faisalabad (UAF) (latitude 31°-04′ N, longitude 73°-06′ E, and an elevation of 184.4 m above sea level) during the winter season of 2020–21. Clay loam soil with pH 8.31, Ec 1.46 dSm⁻¹, available N 0.056%, accessible phosphorus 36.5 ppm, exchangeable potassium 325 ppm, and organic matter 1.08% was used in this experiment to fill the pots. The seeds of two contradicting wheat cultivars, drought-tolerant (cv. Faisalabad-2008) and drought-sensitive (cv. Galaxy-2013), were obtained from the Directorate of Farms, Department of Agronomy, UAF and Seed Department, Ayub Agricultural Research Institute, Faisalabad (AARI).

Analysis of Raw Materials and Vermicompost

The raw material used for the preparation of vermicompost was wheat straw obtained from the Department of Agronomy, UAF. The potential of hydrogen (pH), electrical conductivity (EC), ash content, available nitrogen (N) content, available phosphorus (P), available potassium (K), calcium (Ca), magnesium (Mg), iron (Fe), sulfur (S), and heavy metals such as, cadmium (Cd), nickel (Ni), lead (Pb), mercury (Hg), chromium (Cr), and stannum (Sn) of raw material and vermicompost were measured according to standard protocols and are shown in Tables 1, 2.

TABLE 1

TABLE 1. Analysis of raw material, vermicompost, and cellulolytic microbe–enriched vermicompost.

TABLE 2

TABLE 2. Mean sum of squares regarding the effect of soil-applied wheat straw vermicompost on the growth and physio-biochemical attributes of wheat cultivars under drought stress.

Meteorological Observation

Weather data were gaged throughout the growing period of the wheat crop from the meteorological observatory at Agronomy Department, UAF, and are manifested in Figure 1.

FIGURE 1

FIGURE 1. Meteorological data, mean monthly maximum and minimum temperatures (Tmax and Tmin, respectively), relative humidity (RH), and rainfall during the crop growing season.

Experimental Design and Treatments

This wirehouse experiment consisted of three factors, viz., (a) two contrasting wheat cultivars (Faisalabad-08 and Galaxy-13 as drought-tolerant and drought-sensitive, respectively), (b) three drought levels (control, 70% of field capacity (D0); mild drought, 45% of field capacity (D1); and severe drought, 30% of field capacity (D2)), and (c) different application rates of vermicompost [control (VT₀), 4 t ha⁻¹ (VT₁), 6 t ha⁻¹ (VT₂) and 8 t ha⁻¹ (VT₃)]. The experimental pots were intended under a complete randomized design with factorial arrangements where each pot gets three replicates. Initially, vermicompost was applied before sowing and wheat seeds were sown in uniform soil moisture conditions. After that, water stress treatments were started after first irrigation until harvesting. In each replicate pot, water losses were compensated by applying a measured amount of water to prevail the required field capacity. For each treatment, soil moisture contents were measured on a daily basis by using a soil moisture meter (TZS-W). After 35 days of stress imposition, plant samples, from each replicate pot, were harvested to analyze the morpho-physio-biochemical traits.

Crop Husbandry

The seeds of wheat cultivars were sown in pots (with a measurement of 20 cm × 20 cm), filled with sieved soil 5 kg each, in the rabi season (sown on 06 October 2020). Urea and diammonium phosphate (60 and 57 mg kg⁻¹ soil, respectively) were mixed and applied to each pot at the time of sowing. Initially, fifteen seeds were sown at identical distances; later, ten seedlings were maintained in each replicate pot. Except for treatments, all other practices, including chemical application, were kept uniform for all pots.

Data Recorded

Growth-Related Traits

A rod meter scale was used to calculate the seedling lengths (cm). In order to calculate the seedling weights, first, shoots and roots were separated, and an electrical balance was used to record the fresh weights, which were expressed in g. Then, seedling parts were transferred to an oven at 70 ± 02°C for 72 h and the aforementioned weighing balance was used to calculate the dry weights in g.

Physiological Attributes

Water Potential (Ψw)

At 60 days after sowing, a water potential apparatus (Chas W. Cook and Sons., Birmingham-B, Industrial manufacturer, England) was used to measure the Ψ_w, where a fully emerged flag leaf was used (Scholander et al., 1964). First, pressure-filled gas was exerted into the leaves until the xylem components are visible on the cut section. To ensure no evaporative loss, the samples were taken between 6.00 p.m. and 8.00 a.m., and plant leaves were placed as early as possible in the pressure chamber. From each replicate pot, three samples were taken from each plant, and the average value was measured.

Leaf Osmotic Potential and Turgor Pressure (Ψs & Ψp)

For Ψs, leaves were frozen for seven successive days at −20°C and later, the extracted secretion was used to assess the osmotic potential/solute potential using a vapor pressure osmometer, Wescor-5500. Then, the difference in water and osmotic potential was measured as turgor pressure.

$\left({\mathrm{\psi }}_{\mathrm{P}}\right)=\left({\mathrm{\psi }}_{\mathrm{w}}\right)-\left({\mathrm{\psi }}_{\mathrm{s}}\right).$

Canopy Temperature (°C), Photosynthetic Rate (An), and Transpiration Rate (E)

An infrared temperature sensor (IRIS) was used to measure the canopy temperature. The infrared gas analyzer (IRGA) was used to measure An and E values where five plant leaves in each replicate pot were used, and the average was measured according to the protocol of Singh et al. (2018) and Rosolem et al. (2019).

Stomatal Conductance (gs)

An open system LCA-4 ADC-portable infrared gas analyzer (developed by Analytical Development Company, Hoddeson-England) with the following accommodations: ambient CO₂ concentration-Cref and leaf surface area of 371 μmol mol⁻¹ and 6.25 cm², respectively; the temperature of leaf chamber-Tch varying from 25–28°C; leaf chamber molar gas flow rate-U and leaf chamber volume gas flow rate-v of 400 μmol s⁻¹ and 296 ml min⁻¹, respectively; ambient pressure-P, 97.95 kPa; PAR-Q_leaf at the leaf surface, up to 770 μmol m⁻² s⁻¹ was used to measure gs, and the values were recorded from the 3^rd leaf from the top.

Enzymatic Antioxidants

Enzyme Extraction

First, fresh leaves of 0.5 g were extracted in 50 mM of phosphate buffer solution (with pH 7.8); after that, the extract was then centrifuged through filtration at 16,000 × g for 20 min at 4 ± 0.5°C. Later, the filtrate supernatant was utilized for enzyme assay.

Catalase

A UV–Visible spectrophotometer was used to determine the CAT content, through decomposition and change in absorption by adding hydrogen peroxide at every 30 s interval for 5 min, at a wavelength of 240 nm. The reaction mixture for the estimation of CAT was 900 μl hydrogen peroxide (H₂O₂) solution (5.9 mM) and 2 ml phosphate buffer solution (50 mM). For measuring the enzyme activity, the reaction was initiated by adding an extract of 100 μl of the enzyme to the reaction mixture, and the CAT activity was manifested as μmol of H₂O₂ min⁻¹ mg protein⁻¹, according to the procedure of Chance and Maehly (1955).

Superoxide Dismutase

A method described by Giannopolitis and Ries (1977) based on the use of nitroblue tetrazolium (NBT) was used to determine the SOD activity spectrophotometrically (by using UV–Visible spectrophotometer, IRMECO U2020) at 560 nm wavelength. First, 50 μl of the enzyme extract was added to the working solution containing 50 μM of ethanol-containing NBT solution, 1.3 μM solution of riboflavin, 13 mM of methionine, 75 nM EDTA solution, and 50 Mm phosphate buffer solution (pH 7.8). Then, the solutions were transferred in a chamber under 30-W fluorescent lamp illumination for 5 min, and then the values were measured spectrophotometrically at 560 nm wavelength. The working solution without leaf extract was kept as the control. One unit of SOD was specified as the enzyme amount needed to diminish the rate of reduction of NBT at 560 nm by 50% compared to the reaction solution without plant extract.

Peroxidase

The estimation of POD content was performed by oxidation of guaiacol and specified as a change in absorbance min⁻¹ mg⁻¹ protein. At first, a 100-μl filtrate enzyme extract solution was supplemented with a reaction mixture containing 400 μl of guaiacol solution (20 mM), 500 μL of hydrogen peroxide (H₂O₂) (40 mM), and 2 ml of phosphate (50 mM). Later, the change in absorbance was observed spectrometrically at 470 nm, according to Chance and Maehly (1955).

Statistical Analysis

All accumulated data on wheat growth and morpho-physio-biochemical attributes were computed using Microsoft Excel 2016 and subjected to the analysis of variance technique (an ANOVA). Tukey’s honest significance test (HSD) was used to test the comparative significance of treatments with help of Statistics version 8.1 (Analytical Software ^©, 1985–2005) according to the protocol of Steel et al. (1997). The software SigmaPlot 10.0 was used for graphical presentation.

Results

Morphological Growth

The analyzed data regarding the morphological traits of wheat crop (including seedling length and fresh and dry weights) showed that drought treatments, 45 and 30% FC, significantly (p ≤ 0.05) affected these parameters of both cultivars as compared to well-watered conditions (70% FC) (Table 2; Figure 2). The extent of reduction for these traits was higher in Galaxy-13 than that in Faisalabad-08. Nonetheless, the application of vermicompost significantly promoted the morphological traits of both cultivars under control and drought stress conditions. Significantly higher values of these traits were recorded in plants treated with a high rate of vermicompost (VT₃) than those of the lower ones (VT₀, VT₁, and VT₂). Under severe drought, VT₃ increased root length by 27.57 and 37.19%, shoot length by 16.09 and 31.34%, root fresh weight by 14.74 and 22.90%, shoot fresh weight by 20.69 and 24.39%, root dry weight by 19.44 and 30.76%, and shoot dry weight by 28.57 and 27.26% in Faisalabad-08 and Galaxy-13, respectively. For studied cultivars, under well-watered conditions, Galaxy-13 depicted more biomass than Faisalabad-08; however, both tested cultivars showed a similar trend under drought treatments.

FIGURE 2

FIGURE 2. Effect of soil-applied wheat straw vermicompost (VT) on root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, and shoot dry weight of two wheat cultivars under different drought levels [70% field capacity (FC), 45% FC, and 30% FC]. Bars with different alphabetical letters differ significantly at 5% probability, according to Tukey’s HSD test. VT0, control; VT₁, 4 t ha⁻¹; VT₂, 6 t ha⁻¹; and VT₃ 8 t ha⁻¹.

Physiological Attributes

The physiological attributes (i.e., leaf water potential, osmotic potential, turgor potential, photosynthetic rate, transpiration rate, and stomatal conductance) were significantly reduced under drought stress treatments (45% FC and 30% FC) compared to well-watered condition (70% FC) in both cultivars. Severe drought (30% FC) caused more reduction in plant physiological attributes than moderate drought (45% FC). Among cultivars, the magnitude of reduction for these traits was greater in Galaxy-13 than in Faisalabad-08. However, the vermicompost application significantly affected these traits under control and drought stress in both cultivars where VT₃ recorded significantly higher values followed by other treatments that were ordered as VT₂>VT₁>control (Figure 3). Under severe drought stress, VT₃ treatment increased water potential by 9.43 and 12.17%, osmotic potential by 4.59 and 4.52%, turgor potential by 6.06 and 10.16%, photosynthetic rate by 15.20 and 23.44%, transpiration rate by 14.05 and 26.10%, and stomatal conductance by 14.15 and 13.10% in Faisalabad-08 and Galaxy-13, respectively, than control treatment without vermicompost application. For cultivars under well-watered conditions, the Galaxy-13 recorded significantly higher values than Faisalabad-08. However, under drought treatments, Faisalabad-08 depicted higher values than Galaxy-13, except for canopy temperature. We also noted that drought treatments increased the canopy temperature; however, vermicompost application decreased its values under normal and drought conditions in both cultivars.

FIGURE 3

FIGURE 3. Effect of soil-applied wheat straw vermicompost (VT) on leaf water potential, leaf osmotic potential, leaf turgor potential, canopy temperature, photosynthetic rate, transpiration rate, and stomatal conductance of two wheat cultivars under different drought levels [70% field capacity (FC), 45% FC, and 30% FC]. Bars with different alphabetical letters differ significantly at 5% probability, according to Tukey’s HSD test. VT₀, control; VT₁, 4 t ha⁻¹; VT₂, 6 t ha⁻¹; and VT₃, 8 t ha⁻¹.

Enzymatic Antioxidants

Drought stress and vermicompost application significantly (p ≤ 0.05) affected the activities of antioxidant enzymes including catalase, superoxide dismutase, and peroxidase in both cultivars (Figure 4). Under drought and well-watered conditions, antioxidant enzyme activities were increased by the application of vermicompost where VT₃ recorded the highest increase than other rates in both cultivars. Under severe drought, VT₃ increased superoxide dismutase activity by 8.53 and 11.96%, peroxidase activity by 20.01 and 18.66%, and catalase activity by 27.50 and 6.32% in Faisalabad-08 and Galaxy-13, respectively, than control treatment without vermicompost application. Between tested cultivars, Galaxy-13 depicted statistically similar values compared to those of Faisalabad-08, under sufficient water conditions; however, under drought stress, the Faisalabad-08 cultivar showed better performance than Galaxy-13.

FIGURE 4

FIGURE 4. Effect of soil-applied wheat straw vermicompost on superoxide dismutase, peroxidase, and catalase activities of two wheat cultivars under different drought levels [70% field capacity (FC), 45% FC, and 30% FC]. Bars with different alphabetical letters differ significantly at 5% probability, according to Tukey’s HSD test. VT₀, control; VT₁, 4 t ha⁻¹; VT₂, 6 t ha⁻¹; and VT₃ 8 t ha⁻¹.

Discussion

The current study evaluated the effectiveness of wheat straw vermicompost application in improving plant performance under drought stress conditions. When compared to control, drought stress significantly affected the performance of wheat crop mainly through the production of reactive oxygen species (ROS), which redirected the leaf water potential, caused protein denaturation, and ultimately influenced crop productivity. Prolonged drought also retards plant growth and crop productivity due to severe loss in cell turgidity and dehydration of protoplasm. According to Chen et al. (2011), protoplasm dehydration is directly related to a decrease in cell division. Our results showed that vermicompost application significantly stimulated wheat morphological, physiological, and enzymatic antioxidant activities under drought stress.

It is well-reported that selecting suitable crop cultivars that have standard emergence; morphological, physiological, and antioxidant attributes; and the application of vermicompost are helpful in enhancing crop performance, in terms of better growth and yield under drought stress (Amiri et al., 2017; Ahmad et al., 2021). Similar to these reports, our results indicated that vermicompost application positively influenced the morpho-physiological traits and enzymatic antioxidant activities of both cultivars under drought stress, and the Faisalabad-08 was more productive and proved to be a drought-tolerant cultivar. Drought treatments caused significant reduction in seedling growth of studied wheat cultivars, and the reduction was more prominent in drought-susceptible wheat cultivars (Rahbarian et al., 2011). In this work, wheat straw vermicompost application significantly lowered the detrimental effects of drought. Similar findings were reported in a previous study where the authors have reported that vermicompost, when applied at a high rate, rendered more positive effects under control and drought conditions (Rahbarian et al., 2011; Kiran, 2019). Under drought stress, higher dehydration, denaturation of proteins, and excessive production of ROS caused significant reduction in seedling growth (Boyer and Westgate, 2004). Severe drought caused more reduction in seedling lengths and higher rates of vermicompost were more effective in reducing the negative effects of stress (Garcia et al., 2012). Similar findings were reported in our results.

Leaf water potential, osmotic and turgor potential, photosynthetic and transpiration rates, and stomatal conductance are the important indicators to determine the stress effects (Garcia et al., 2012; Kiran, 2019). In this study, moderate and severe drought resulted in significant decrease in the abovementioned physiological traits of both cultivars when compared to control, where drought-sensitive cultivars recorded a greater decrease than other studied varieties. Similar findings were reported in previous studies in which authors have reported that drought-resistant varieties had higher leaf water, osmotic and turgor potentials, photosynthetic and transpiration rates, and stomatal conductance (Gunes et al., 2007). In our work, the drought-resistant variety Faisalabad-08 exhibited better physiological traits under drought stress for all vermicompost treatments than the sensitive one Galaxy-13. Plants with higher water potential, turgor potential, stomatal conductance, and photosynthetic rates can store more assimilates for better growth and productivity. In stressed plants, reduced water losses help in stomatal closure for better tolerance to drought (Li-Ping et al., 2006; Sakuma et al., 2006).

In this work, drought significantly lowered water and osmotic potentials. Similar findings were reported in a previous study by Xue and Loveridge (2004) who found that drought had decreased osmotic potential owing to decreased water availability than the untreated control. In addition, we found that vermicompost application increased the osmotic potential owing to re-established turgor pressure. Similarly, Kashem et al. (2015) reported that maintaining turgor pressures is crucial for proper cell growth, expansion, and stomatal conductivity.

The application of vermicompost significantly alleviated drought stress by increasing the concentrations of scavenging enzymes including SOD, POD, and CAT. Similar findings were reported by Hussain et al. (2010), where the authors have reported that higher antioxidant activities positively reduced oxidative damage caused by overproduction of activated oxygen species. In this work, a high rate of vermicompost significantly increased the plant tolerance to drought-induced oxidative stress by increasing the concentrations of antioxidant enzymes in both cultivars. The findings are consistent with those of Nazarideljou and Heidari (2014), who demonstrated that vermicompost application increased SOD, POD, and CAT activity.

Conclusion

Soil-applied vermicompost is an effective approach to alleviate drought damage in wheat. In response to drought stress, vermicompost application, particularly at a high rate, considerably promoted the growth and physiological attributes in Faisalabad-08 and Galaxy-13. Wheat genotypes, thereby, lead to better growth performance under drought stress. The upregulation of antioxidant enzymes such as catalase, peroxidase, and superoxide dismutase activities may also have contributed to better drought tolerance in wheat. Wheat straw vermicompost–induced improvement in physiological traits played a key role in improving maize growth under drought stress. This study advocates the possibility of soil-applied wheat straw vermicompost to improve drought tolerance by promoting the growth, physiology, and antioxidant defense system.

Data Availability Statement

The raw data supporting the conclusion of this article will be made available by the authors without undue reservation.

Author Contributions

All authors listed have made a substantial, direct, and intellectual contribution to the work and approved it for publication.

Funding

The current work was funded by the Taif University Researchers Supporting Project Number (TURSP-2020/94), Taif University, Taif, Saudi Arabia.

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s Note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors, and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Acknowledgments

The results presented in this study are a part of PhD studies by AA. The authors gratefully acknowledge HEC for providing financial support to carry out this research work under HEC projects “NRPU-HEC project no. 7527/Punjab/NRPU/R&D/HEC/2017_Vermicomposting: A resourceful organic fertilizer to improve agriculture production and soil health and second project “Vermicomposting: An Agricultural Waste Management Technology,” Pak-Turk Researchers Mobility Grant Program Phase- II, vide letter No. (Ph-II-MG-9)/PAKTURK/R&D/HEC/2018.” The authors also extend their sincere appreciation to Taif University for funding the current work by Taif University Researchers Supporting Project number (TURSP-2020/94), Taif University, Taif, Saudi Arabia.

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