In the original article, there was a mistake in Table S3 as published. The sequence of Read2_SP, the Read2 sequencing primer, was incorrect. The corrected Table S3 appears below.
Table S3
| Primer name | Function in FB5P-seq | Sequence 5′-3′ |
|---|---|---|
| (dT)30_Smarter | Prime Reverse Transcription at 3′ ends of polyadenylated mRNA and introduce PCR handle | TGCGGTATCTAAAGCGGTGAGTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTVN |
| TSO_BCx_UMI5_TATA | Perform template switching and introduce 8 nt. well-specific barcode & 5 nt. UMI | AGACGTGTGCTCTTCCGATCTXXXXXXXXNNNNNTATArGrGrG |
| Satija_PCR | Forward primer for LD-PCR amplification of cDNA libraries | AGACGTGTGCTCTTCCGATCT |
| SmarterR | Reverse primer for LD-PCR amplification of cDNA libraries | TGCGGTATCTAAAGCGGTGAG |
| i7_BC1 | Forward primer for 5′-end enrichment of tagmented library and incorporation of plate-specific barcode | CAAGCAGAAGACGGCATACGAGATCCTGGTAGGTGACTGGAG TTCAGACGTGTGCTCTTCCGATCT |
| i7_BC2 | CAAGCAGAAGACGGCATACGAGATTAAGCATGGTGACTGGAG TTCAGACGTGTGCTCTTCCGATCT | |
| i7_BC3 | CAAGCAGAAGACGGCATACGAGATAGATGTGCGTGACTGGAG TTCAGACGTGTGCTCTTCCGATCT | |
| i7_BC4 | CAAGCAGAAGACGGCATACGAGATGTCGAGCAGTGACTGGAG TTCAGACGTGTGCTCTTCCGATCT | |
| i7_BC5 | CAAGCAGAAGACGGCATACGAGATGAATTGCTGTGACTGGAG TTCAGACGTGTGCTCTTCCGATCT | |
| i7_BC6 | CAAGCAGAAGACGGCATACGAGATAAGCAACTGTGACTGGAG TTCAGACGTGTGCTCTTCCGATCT | |
| i7_primer | Forward primer for amplification of sequencing library | CAAGCAGAAGACGGCATACGA |
| Read1_SP | Read1 sequencing primer | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG |
| i7_SP | Index Read i7 sequencing primer | AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC |
| Read2_SP | Read2 sequencing primer | GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT |
Primers used in FB5P-seq.
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
Summary
Keywords
single-cell RNA sequencing, transcriptome, antigen receptor, B cells, T cells
Citation
Attaf N, Cervera-Marzal I, Dong C, Gil L, Renand A, Spinelli L and Milpied P (2020) Corrigendum: FB5P-seq: FACS-Based 5-Prime End Single-Cell RNA-seq for Integrative Analysis of Transcriptome and Antigen Receptor Repertoire in B and T Cells. Front. Immunol. 11:1521. doi: 10.3389/fimmu.2020.01521
Received
08 June 2020
Accepted
09 June 2020
Published
17 July 2020
Approved by
Frontiers Editorial Office, Frontiers Media SA, Switzerland
Volume
11 - 2020
Updates
Copyright
© 2020 Attaf, Cervera-Marzal, Dong, Gil, Renand, Spinelli and Milpied.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Pierre Milpied milpied@ciml.univ-mrs.fr
‡Present address: Iñaki Cervera-Marzal, Eura Nova, Marseille, France
This article was submitted to B Cell Biology, a section of the journal Frontiers in Immunology
†These authors have contributed equally to this work
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