In the published article, there was an error in affiliation 1. Instead of “Ministry of Agriculture” the correct name of the ministry is “Ministry of Agriculture and Rural Affairs”.
In Table 1, the references for “53813,” “GEB88,” and “pSW7848,” were incorrectly written as “This lab.” It should be “Le Roux et al., 2007,” “Nguyen et al., 2018,” and “Val et al., 2012,” respectively. Additionally, the intermediate host Escherichia coli strain was named as “GEB802,” but should be “53813.”
The corrected Table 1 appears below.
Table 1
| Strains or plasmids | Relevant characteristics | Sources |
|---|---|---|
| V. alginolyticus | ||
| ZJ-T | Apr (ampicillin resistant), translucent/smooth variant of wild strain ZJ-51 (Xiaochun et al., 2017); isolated from diseased Epinephelus coioides off the Southern China coast | Chang et al., 2009 |
| ZJ-T-Δsrvg23535 | Apr; ZJ-T carrying an deletion of srvg23535 | This study |
| E. coli | ||
| Π3813 | Emrr, Tcr, lacIQ, thi1, supE44, endA1, recA1, hsdR17, gyrA462, zei298::tn10[Tc], ΔthyA:: (erm-pir116); the intermediate host of suicide vector pSW7848 | Le Roux et al., 2007 |
| GEB883 | Eryr, Tetr, WT E. coli K12 ΔdapA::erm pir RP4-2 ΔrecA gyrA462, zei298::Tn10; donor strain for conjugation | Nguyen et al., 2018 |
| Plasmids | ||
| pSW7848 | Cmr; suicide vector with an R6K origin, requiring the Pir protein for its replication, and the ccdB toxin gene | Val et al., 2012 |
| pSW7848-Δsrvg23535 | Cmr; pSW848 containing the mutant allele of Δsrvg23535 | This study |
Strains and plasmids used in this study.
A correction has also been made to the MATERIALS AND METHODS, Bacterial Strains, Plasmids, and Growth Conditions and Gene Disruption, paragraph one:
“To generate the sRNA disruptant, the sequence from 46 bp before the 5′ end to 2 bp after the 3′ end was deleted from the chromosome of V. alginolyticus ZJ-T. The deletion was constructed by homologous recombination as described before with some modification (Yiqin et al., 2016). Briefly, two flanking fragments of srvg23535 (Figure 1A) were amplified with two pairs of primers, srvg23535-UP-F and -R and srvg23535-DOWN-F and -R respectively, and the linearized pSW7848 was amplified with pSW7848-F and -R (Supplementary Table 1). srvg23535-UP-F and srvg23535-DOWN-R contained overlapping extensions with pSW7848-R and -F, respectively, and srvg23535-UP-R contained overlapping extensions with srvg23535-DOWN-F. The two flanking fragments were further assembled into the linearized pSW7848 by using a ClonExpress Multis One Step Cloning Kit (Vozyme, China), generating the recombinant plasmid pSW7848-Δsrvg23535 comprising the 1,084 bp upstream and 1,105 bp downstream regions of srvg23535 (Table 1), using E. coli Π3813 as an intermediate host. The recombinant plasmid was transferred by conjugation from strain GEB883 (Table 1) to V. alginolyticus ZJ-T before allelic exchange as described above. The sRNA disruptant was then confirmed by sequencing and the strain was named ZJ-T-Δsrvg23535 (Figure 1 and Table 1).”
The authors apologize for these errors and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
Statements
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
References
1
ChangC.JinX.ChaoqunH. (2009). Phenotypic and genetic differences between opaque and translucent colonies of Vibrio alginolyticus. Biofouling25, 525–531. 10.1080/08927010902964578
2
Le RouxF.BinesseJ.SaulnierD.MazelD. (2007). Construction of a Vibrio splendidus mutant lacking the metalloprotease gene vsm by use of a novel counterselectable suicide vector. Appl. Environ. Microbiol. 73, 777–784. 10.1128/AEM.02147-06
3
NguyenA. N.DisconziE.CharrièreG. M.Destoumieux-GarzónD.BoulocP.Le RouxF.et al. (2018). csrB gene duplication drives the evolution of redundant regulatory pathways controlling expression of the major toxic secreted metalloproteases in Vibrio tasmaniensis LGP32. mSphere3:e00582-18. 10.1128/mSphere.00582-18
4
ValM. E.SkovgaardO.Ducos-GalandM.BlandM. J.MazelD. (2012). Genome engineering in Vibrio cholerae: a feasible approach to address biological issues. PLoS Genet. 8:e1002472. 10.1371/journal.pgen.1002472
5
XiaochunH.ChangC.ChunhuaR.YingyingL.YiqinD.YiyingY.et al. (2017). Identification and characterization of a locus putatively involved in colanic acid biosynthesis in Vibrio alginolyticus ZJ-51. Biofouling34, 1–14. 10.1080/08927014.2017.1400020
6
YiqinD.ChangC.ZheZ.JingjingZ.JacqA.XiaochunH.et al. (2016). The RNA chaperone Hfq is involved in colony morphology, nutrient utilization and oxidative and envelope stress response in Vibrio alginolyticus. PLoS ONE11:e0163689. 10.1371/journal.pone.0163689
Summary
Keywords
small non-coding RNAs, srvg23535, Vibrio alginolyticus, identification, Phenotype MicroArray technology
Citation
Deng Y, Su Y, Liu S, Guo Z, Cheng C, Ma H, Wu J, Feng J and Chen C (2019) Corrigendum: Identification of a Novel Small RNA srvg23535 in Vibrio alginolyticus ZJ-T and Its Characterization With Phenotype MicroArray Technology. Front. Microbiol. 10:21. doi: 10.3389/fmicb.2019.00021
Received
14 December 2018
Accepted
09 January 2019
Published
31 January 2019
Volume
10 - 2019
Edited and reviewed by
Eric Altermann, AgResearch, New Zealand
Updates
Copyright
© 2019 Deng, Su, Liu, Guo, Cheng, Ma, Wu, Feng and Chen.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Juan Feng juanfeng@scsfri.ac.cnChang Chen chen.chang@scsio.ac.cn
This article was submitted to Evolutionary and Genomic Microbiology, a section of the journal Frontiers in Microbiology
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