%A Fernández-Fernández,Rosa
%A Lozano,Carmen
%A Eguizábal,Paula
%A Ruiz-Ripa,Laura
%A Martínez-Álvarez,Sandra
%A Abdullahi,Idris Nasir
%A Zarazaga,Myriam
%A Torres,Carmen
%D 2022
%J Frontiers in Microbiology
%C
%F
%G English
%K bacteriocin,Multidrug resistance (MDR) bacteria,BLIS activity,Coagulase - negative Staphylococcus,Coagulase positive staphylococci
%Q
%R 10.3389/fmicb.2022.870510
%W
%L
%M
%P
%7
%8 2022-April-26
%9 Original Research
%#
%! bacteriocin-like in staphylococci active against MDR bacteria
%*
%<
%T Bacteriocin-Like Inhibitory Substances in Staphylococci of Different Origins and Species With Activity Against Relevant Pathogens
%U https://www.frontiersin.org/articles/10.3389/fmicb.2022.870510
%V 13
%0 JOURNAL ARTICLE
%@ 1664-302X
%X Bacteriocins are antimicrobial peptides with relevance in the modulation of human and animal microbiota that have gained interest in biomedical and biotechnological applications. In this study, the production of bacteriocin-like inhibitory substances (BLIS) was tested among a collection of 890 staphylococci of different origins (humans, animals, food, and the environment) and species, both coagulase-positive (CoPS, 238 isolates of 3 species) and coagulase-negative staphylococci (CoNS, 652 isolates of 26 species). Of the 890 staphylococci, 60 (6.7%) showed antimicrobial activity by the spot-on-lawn method against at least one of the 25 indicator bacteria tested. BLIS-producer (BLIS+) isolates were detected in 8.8% of CoPS and 6.0% of CoNS. The staphylococcal species with the highest percentages of BLIS+ isolates were S. chromogenes (38.5%), S. pseudintermedius (26.7%), and S. warneri (23.1%). The production of BLIS was more frequently detected among isolates of pets, wild animals, and food. Moreover, 13 BLIS+ isolates showed wide antimicrobial activiy spectrum, and 7 of these isolates (of species S. aureus, S. pseudintermedius, S. sciuri, and S. hominis) demonstrated antimicrobial activity against more than 70% of the indicator bacteria tested. The genetic characterization (by PCR and sequencing) of the 60 BLIS+ isolates revealed the detection of (a) 11 CoNS and CoPS isolates carrying putative lantibiotic-like genes; (b) 3 S. pseudintermedius isolates harboring the genes of BacSp222 bacteriocin; and (c) 2 S. chromogenes isolates that presented the gene of a putative cyclic bacteriocin (uberolysin-like), being the first report in this CoNS species. Antimicrobial susceptibility testing was performed in BLIS+ isolates and one-third of the CoNS isolates showed susceptibility to all antibiotics tested, which also lacked the virulence genes studied. These BLIS+ CoNS are good candidates for further characterization studies.