CORRECTION article

Front. Microbiol., 27 April 2022

Sec. Extreme Microbiology

Volume 13 - 2022 | https://doi.org/10.3389/fmicb.2022.903233

Corrigendum: Differences in Bioenergetic Metabolism of Obligately Alkaliphilic Bacillaceae Under High pH Depend on the Aeration Conditions

  • 1. Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo, Japan

  • 2. Graduate School of Agriculture, Hokkaido University, Sapporo, Japan

  • 3. College of Industrial Technology, Nihon University, Narashino, Japan

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In the original article, there was a mistake in the legend for Figure 2 as published. It described that “The acid residue-abundant segment of Glu25-Lys46 (Glu6-Lys27 in the processed protein base) in S. cohnii DSM 6307T is indicated by green box” at L2 –L3. The correct legend appears below.

Figure 2. Amino acid sequence alignment of membrane bound cytochrome c-550 from Evansella clarkii and other alkaliphilic and neutralophilic Bacillaceae. Asn (N)-rich segment, Asn (N)21-Asn (N)43 [Asn (N)4-Asn (N)26 in the processed protein base] in the E. clarkii cytochromes c is indicated by red box. The acid residue-abundant segment of Glu25-Lys46 (Glu6-Lys27 in the processed protein base) in S. cohnii DSM 6307T is indicated by red box. N-terminal amino acid residue in processed cytochrome c, [Cys (C)] is indicated by blue marker. The blue boxed sequences are heme sequences representing heme-binding site and axical ligands (H and M). Acidic and basic residues are indicated by blue and red letters. Although A. pseudofirmus OF4 is a facultative alkaliphile, the species A. pseudofirmus and the presented cytochrome c sequence are classified in obligate alkaliphile. Although Sporosarcina pasteurii is an obligate alkaliphile, its cytochrome c sequence is similar to those in the facultative alkaliphilic strains.

In the original article, there was a mistake in Table 2 as published. The unit for respiratory rate was incorrect. The corrected Table 2 appears below.

Table 2

Growth rate (μmax)1Cytochrome c content (nmol ·mg−1)1ΔΨ (mV)1,2Respiratory rate (μmol ·O2 min−1 mg−1)2H+/O1Maximum ATP synthesis rate2 (nmol ·min−1 ·mg−1)2
E. clarkii DSM8720T
Low aeration
0.360.89 ± 0.07−135 ± 8ND0.6 ± 0.1 (6)§ND
E. clarkii DSM 8720T
High aeration
0.420.36 ± 0.01−192 ± 30.19 ± 0.042.2 ± 0.2 (6)26.2 ± 1.7
S. cohnii YN-2000
High aeration
0.380.62 ± 0.01−173 ± 50.20 ± 0.032.6 ± 0.3 (6)9.6 ± 0.9
B. subtilis IAM 1026
Low aeration
0.260.18 ± 0.06−133 ± 13ND2.8 ± 0.8 (6)ND
B. subtilis IAM 1026
High aeration
0.550.21 ± 0.02−121 ± 70.50 ± 0.064.9 ± 0.1 (6)2.0 ± 0.6

Summary for high aeration and low aeration in obligate alkaliphilic Evansella clarkii, facultative alkaliphilic Sutcliffiella cohnii, and neutralophilic Bacillussubtilis*.

*

These data cited from

1

Goto (2006) and

2

Goto et al. (2016).

§

The numbers in parentheses are based on the theoretically extruded H+ in 1/2O2 consumption by the respiratory chain.

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

Publisher's Note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

References

  • 1

    GotoT. (2006). Functional Analyses of the Membrane of Alkaliphilic Bacillusspp. for Their Environmental Adaptations. [dissertation/Master's thesis]. Sapporo: Hokkaido University.

  • 2

    GotoT.HirabayashiT.MorimotoH.YamazakiK.InoueN.MatsuyamaH.et al. (2016). Contribution of intracellular negative ion capacity to donnan effect across the membrane in alkaliphilic Bacillus spp. J. Bioeng. Biomembr. 48, 8796. 10.1007/s10863-015-9641-9

Summary

Keywords

bioenergetic mechanism, membrane electrical potential, Bacillaceae, Evansella clarkii, Donnan effect, H+ capacitor, cytochrome c, alkaliphilic

Citation

Goto T, Ogami S, Yoshimume K and Yumoto I (2022) Corrigendum: Differences in Bioenergetic Metabolism of Obligately Alkaliphilic Bacillaceae Under High pH Depend on the Aeration Conditions. Front. Microbiol. 13:903233. doi: 10.3389/fmicb.2022.903233

Received

24 March 2022

Accepted

05 April 2022

Published

27 April 2022

Approved by

Frontiers Editorial Office, Frontiers Media SA, Switzerland

Volume

13 - 2022

Updates

Copyright

*Correspondence: Isao Yumoto

This article was submitted to Extreme Microbiology, a section of the journal Frontiers in Microbiology

Disclaimer

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

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