Original Research ARTICLE
Gastrodin alleviates oxidative stress-induced apoptosis and cellular dysfunction in human umbilical vein endothelial cells via the Nrf2/HO-1 pathway and accelerates wound healing in vivo
- 1Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, China
- 2School of Second Clinical Medical, Wenzhou Medical University, China
- 3The Second School of Medicine, Wenzhou Medical University, China
Angiogenesis, primarily carried out by endothelial cells, is an important biological process in wound healing. Excessive levels of reactive oxygen species (ROS) produced at wound sites cause endothelial cell dysfunction, leading to delayed wound healing. Gastrodin (GAS), the major active component of Gastrodia elata, has been previously reported to possess antioxidative effects. In our study, the protective effect of GAS on human umbilical vein endothelial cells (HUVECs) under oxidative stress and its function in wound healing were investigated. The results show that treating HUVECs with GAS attenuated tert-butyl hydroperoxide (TBHP)-induced apoptosis and cellular dysfunction, including cellular tube formation, migration, and adhesion. Mechanistically, we found that GAS protects HUVECs from TBHP-induced cellular apoptosis by activating the nuclear factor (erythroid-derived 2)-like 2 (Nrf2)/haem oxygenase 1 (HO-1) pathway. An in vivo study illustrated that the oral administration of GAS enhances vascularization in regenerated tissue and facilitates wound healing. The findings of this study demonstrated that GAS may serve as a potential agent that accelerates wound healing.
Keywords: Gastrodin, Wound Healing, Angiogenesis, Apoptosis, Nrf2/HO-1
Received: 21 Jul 2019;
Accepted: 04 Oct 2019.
Copyright: © 2019 Lin, Shi, Liao, Wu, Lin, Wu, Zeng, Qi, Liu, Jin and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Dr. Haiming Jin, Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China, firstname.lastname@example.org
Prof. Xiangyang Wang, Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China, email@example.com