In the published article, there was an error for the units used to report the press juice concentration. The units were incorrectly written as µg/mL, instead of mg/mL. In the original article, this error appeared in the legend for Figure 4, in Figures 1–3 and in the Materials and Methods and Results sections.
A correction has been made to Materials and Methods, Phosphorylation Experiments, Paragraph number 1. This sentence previously stated:
“hTERT-C3 cells (4.7 × 104 cell/mL) were seeded into 6-well plates three to 4 days before experiments were performed. Once 90% confluence was reached, cells were treated with OT (100 nM) for 2, 5, 15, 30 or 45 min. To investigate the modulation of OT-induced phosphorylation under these conditions, cells were pre-treated with BPJ (2% corresponding to 20 μg/mL), atosiban (100 nM) or just medium. In additional experiments, cells were pre-treated with BPJ (20 μg/mL), BEF (2.20 μg/mL), FEF (17.39 μg/mL), A-Mix (0.68 μg/mL), BEF plus FEF and BEF plus A-Mix (same concentrations as in the single treatments), or just medium for 30 min before stimulation with OT for 5 min.”
The corrected sentence appears below:
“hTERT-C3 cells (4.7 × 104 cell/mL) were seeded into 6-well plates three to 4 days before experiments were performed. Once 90% confluence was reached, cells were treated with OT (100 nM) for 2, 5, 15, 30 or 45 min. To investigate the modulation of OT-induced phosphorylation under these conditions, cells were pre-treated with BPJ (2% corresponding to 20 mg/mL), atosiban (100 nM) or just medium. In additional experiments, cells were pre-treated with BPJ (20 mg/mL), BEF (2.20 μg/mL), FEF (17.39 μg/mL), A-Mix (0.68 μg/mL), BEF plus FEF and BEF plus A-Mix (same concentrations as in the single treatments), or just medium for 30 min before stimulation with OT for 5 min.”
The corrected Figure 1, Figure 2 and Figure 3 and the corresponding captions appear below.
FIGURE 1
FIGURE 2
FIGURE 3
The corrected Figure 4 caption appears below.
“FIGURE 4 Effect of BPJ fractions/compounds and atosiban on OT-induced MAPKs phosphorylation. In the time-course experiments, hTERT-C3 cells were pre-treated with or without 20 mg/mL BPJ or 100 nM atosiban for 30 min, before incubation with 100 nM OT for 2, 5, 15, 30, and 45 min (A, C, E). To compare the effects of BPJ and the various fractions/compounds, cells were pre-treated with 20 mg/mL BPJ, 2.20 μg/mL BEF, 17.39 μg/mL FEF, 0.68 μg/mL A-Mix, BEF plus FEF, or BEF plus A-Mix (same concentrations as for single fractions) for 30 min before stimulation with OT for 5 min (B, D, F). Whole cell proteins were subjected to western blot analysis with antibodies against phosphorylated p38 (A, B), SAPK/JNK (C, D) and ERK1/2 (E, F); matching densitometry analyses are depicted bellow the representative blots. Samples from the same experiment were processed in parallel and membranes were probed with GAPDH to confirm equal loading. Blot images are from a representative experiment, and line between bands delineate boundary between the gels that were cropped. Values represent the mean ± SEM of four independent experiments; *p < 0.05 compared with control; #p < 0.05 compared to OT-treated.”
A correction has been made to Results, BPJ inhibits OT-induced rise of [Ca2+]i in PHM1-41 myometrial cells, Paragraph number 1. These sentences previously stated:
“As previously shown, the exposure of hTERT-C3 cells to 100 nM OT induced an increase of [Ca2+]i with a peak response at about 10–20 s after stimulation, and a subsequent decrease to resting conditions (Figures 3A,B). Pre-incubation with BPJ (0.1%–2.0% corresponding to 1.0–20.0 μg/mL) led to a concentration dependent decrease of the [Ca2+]i peak induced by OT (p < 0.0001; Figure 3A). To verify if this effect was cell line dependent, experiments were conducted in a second myometrial cell line (PHM1-41). A peak response of [Ca2+]i was observed at 12–20 s after stimulation with OT (Figures 3C,D). When pre-incubated with BPJ, a decrease of the OT-induced increase of cytosolic [Ca2+]i peak was observed (Figure 3C). BPJ thus promoted a concentration-dependent effect on Δ[Ca2+]i in both cell lines (p < 0.0001; Figure 3E), with significant effects at concentrations >2.5 μg/mL in hTERT-C3 cells (p = 0.012), and >5.0 μg/mL in PHM1-41 cells (p = 0.026).”
The corrected sentence appears below:
“As previously shown, the exposure of hTERT-C3 cells to 100 nM OT induced an increase of [Ca2+]i with a peak response at about 10–20 s after stimulation, and a subsequent decrease to resting conditions (Figures 3A,B). Pre-incubation with BPJ (0.1%–2.0% corresponding to 1.0–20.0 mg/mL) led to a concentration dependent decrease of the [Ca2+]i peak induced by OT (p < 0.0001; Figure 3A). To verify if this effect was cell line dependent, experiments were conducted in a second myometrial cell line (PHM1-41). A peak response of [Ca2+]i was observed at 12–20 s after stimulation with OT (Figures 3C,D). When pre-incubated with BPJ, a decrease of the OT-induced increase of cytosolic [Ca2+]i peak was observed (Figure 3C). BPJ thus promoted a concentration-dependent effect on Δ[Ca2+]i in both cell lines (p < 0.0001; Figure 3E), with significant effects at concentrations >2.5 mg/mL in hTERT-C3 cells (p = 0.012), and >5.0 mg/mL in PHM1-41 cells (p = 0.026).”
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
Statements
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Summary
Keywords
Bryophyllum pinnatum, Kalanchoe pinnata, myometrium cells, oxytocin, MAPK, intracellular calcium, cell signaling
Citation
Santos S, Zurfluh L, Mennet M, Potterat O, von Mandach U, Hamburger M and Simões-Wüst AP (2023) Corrigendum: Bryophyllum pinnatum compounds inhibit oxytocin-induced signaling pathways in human myometrial cells. Front. Pharmacol. 14:1141346. doi: 10.3389/fphar.2023.1141346
Received
10 January 2023
Accepted
20 January 2023
Published
08 February 2023
Volume
14 - 2023
Edited and reviewed by
Sabina Passamonti, University of Trieste, Italy
Updates
Copyright
© 2023 Santos, Zurfluh, Mennet, Potterat, von Mandach, Hamburger and Simões-Wüst.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Ana Paula Simões-Wüst, anapaula.simoes-wuest@usz.ch
This article was submitted to Drug Metabolism and Transport, a section of the journal Frontiers in Pharmacology
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.