Biodecolorization of Reactive Red 120 Azo Dye by Metal-Ion Tolerant Lysinibacillus capsici Bacteria and its Application Potential in Textile Effluents Treatment

Kusum Lata¹Rajat Pratap Singh¹Dr. Balram Ambade²Ashish Kumar^1*

• ¹Guru Ghasidas Vishwavidyalaya, Bilaspur, India
• ²National Institute of Technology Jamshedpur, Jamshedpur, India

Currently, the textile industry is among the most rapidly expanding areas of the economy and is an important source of water pollution. There are many efficient chemical and physical methods for treating textile effluent, but they produce secondary pollutants. Therefore, there is a need to manage the textile waste wastewater. The potential Lysinibacillus capsici bacteria strain has been isolated from the bark borer insect tunnel from Peltophorum pterocarpum plant, and has been determined to be effective in > 95 decolorizing Reactive Red 120, and other azo dyes such as AB 113 85%, orange II 94.62%, Congo red 94.62%, phenol red 94.54%, and mixtures of azo dyes 81.66%. Physico-chemical factors were optimized manually, including Taguchi design. Fabric discolorations of L. capsici were qualitatively studied. The FTIR, GC-MS, and UV absorbance studies also confirmed that the dye had been broken into its amines. Research findings using enzyme assays have shown that the bacteria Lysinibacillus capsici can utilize laccase and manganese peroxidase and are capable of degrading dyes significantly. According to this work, immobilized L. capsici cells and the studied four bacterial consortiums, Lysinibacillus capsici, Alcaligenes faecalis subsp. Phenolicus, Acinetobacter baumanni, and Pseudomonas aeruginosa may be employed to degrade RR120 effectively, and it is concluded that L. capsici bacteria is significantly efficient in textile effluent treatment.