ORIGINAL RESEARCH article
Front. Oncol.
Sec. Thoracic Oncology
Volume 15 - 2025 | doi: 10.3389/fonc.2025.1665342
This article is part of the Research TopicMolecular Pathways and Signaling Molecules in Cancer Therapy: Advances and InnovationsView all 12 articles
Hsp70 regulates CD24 expression and promotes metastasis and invasion of lung cancer via the MAPK/ERK signaling pathway
Provisionally accepted
- 1The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, China
- 2Bayannur Hospital, Bayannur, China
- 3University of Rochester, Rochester, United States
- 4Youjiang Medical University for Nationalities, Baise, China
- 5Department of Neurology, the Affiliated Hospital of Inner Mongolia Medical University, Huhhot, China
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Purpose: To investigate the effects and mechanisms of Hsp70-mediated regulation of CD24 expression on the invasion and metastasis of lung cancer. Methods: Protein-protein interactions between Hsp70 and CD24 were analyzed by co-immunoprecipitation and immunofluorescence. Lentiviral-mediated Hsp70 knockdown and CD24 overexpression were validated using RT-qPCR and Western blotting. Functional consequences were assessed through CCK-8 proliferation assays, colony formation, wound healing, Transwell migration/invasion, and angiogenesis assays. In vivo metastatic potential was evaluated using a tail vein injection model in nude mice. Results: Hsp70 and CD24 demonstrated interaction through co-precipitation. Knockdown of Hsp70 significantly attenuated CD24 expression (p<0.01), whereas CD24 overexpression did not alter Hsp70 levels. Phenotypically, Hsp70 suppression impaired cellular proliferation, migration, invasion and angiogenic capacity, while CD24 overexpression potentiated these oncogenic properties. Both manipulations modulated ERK1/2, MEK, Raf and Ras phosphorylation status. Conclusion: Hsp70 upregulates CD24 expression and activates downstream MAPK/ERK signaling, thereby enhancing the metastatic and invasive capacity of lung cancer.
Keywords: Hsp70, CD24, lung cancer, MAPK/ERK Pathway, Mechanism & characterization
Received: 24 Jul 2025; Accepted: 06 Oct 2025.
Copyright: © 2025 Liu, Yu, Hu, Zhang, Zhang, Liu, Jiaqi and Qiao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Xiaojuan Qiao, xiaojuanqiao@126.com
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.