Serum levels of Per-and polyfluoroalkylated substances and methylation of DNA from peripheral blood

Hanane Omichessan^1,2Dzevka Dragic^2,3,4Vittorio Perduca^2,5Thérèse Truong²Silvia Polidoro⁶Marina Kvaskoff²German Cano-Sancho⁷Jean-Philippe Antignac⁷Laura Baglietto⁸Francesca Romana Mancini²Gianluca Severi^2,9*

• ¹Université Paris-Saclay, Saint Aubin, France
• ²Université Paris-Saclay, UVSQ, Inserm "Exposome, inheritance, cancer and health" team, CESP U1018, Gustave Roussy, Villejuif, France
• ³Department of Social and Preventive Medicine, Faculty of Medicine, Laval University, Quebec, Canada
• ⁴Centre de recherche sur le cancer de l’Université Laval, Centre de recherche du CHU de Québec, Université Laval, Québec, Quebec, Canada
• ⁵Université Paris Cité, CNRS, MAP5, Paris, France
• ⁶Department of Translational Medicine, University of Eastern Piedmont, Novara, Piedmont, Italy
• ⁷Oniris, INRAE, LABERCA, Nantes, France
• ⁸Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Tuscany, Italy
• ⁹Department of Statistics, Computer Science, Applications "G. Parenti" (DISIA), University of Florence, Florence, Italy

Background. Perfluorooctanoic acid (PFOA) and Perfluorooctane sulfonate (PFOS) are among numerous chemicals in the Per-and polyfluoroalkylated substances (PFAS) group, which are commonly present in various consumer and industrial products. These chemicals are recognized for their persistency, the ability to accumulate in biological systems and their documented adverse effects on human health . Previous research, which has primarily centered on global methylation patterns, has suggested that some effects of PFAS on human health may be linked to modifications in DNA methylation (DNAm). The aim of our study was to assess the relationship between the serum levels of PFOS and PFOA and CpG site-specific methylation of DNA from peripheral blood.Methods. We used a case-control study on breast cancer nested within the E3N cohort, a prospective study of French women, in which we measured DNAm at more than 850,000 CpG sites with the Illumina Infinium MethylationEPIC BeadChip for 166 case-control pairs. Serum levels of PFOS and PFOA were measured by liquid chromatography coupled to tandem mass spectrometry.Results. We found 64 CpG sites with significant hypomethylation or hypermethylation associated with increased levels of PFOA or PFOS (p-value Bonferroni <0.05). The strongest association was found between PFOA serum levels and decreased DNAm at cg06874740 (p-value Bonferroni = 2.2x10 -5 ) and between PFOS serum levels and decreased DNAm at cg02793158 (p-value Bonferroni = 9.3x10 -5 ). Gene-set enrichment analyses using all CpG sites associated with PFOA or PFOS with an unadjusted p-value <0.01, identified 20 KEGG pathways for each of these compounds.Conclusions. PFAS exposure may be linked to substantial and widespread changes in the methylome that may be involved in the consequences on health of these pollutants. Our findings indicate that the biological and health effects of PFOA and PFOS may be more intricate and varied than previously thought, reinforcing the need for policies aimed at regulating this class of endocrine-disrupting chemicals.