A Retraction of the Original Research Article
Tobacco smoking affects the salivary gram-positive bacterial population
by Grine, G., Royer, A., Terrer, E., Diallo, O. O., Drancourt, M., and Aboudharam, G. (2019). Front. Public Health 7:196. doi: 10.3389/fpubh.2019.00196
The journal retracts the 19 July 2019 article cited above.
Following publication, it was brought to our attention that the ethical approval for this study referred to the same ethics approval number 2016-011 that had been used across a series of unrelated published studies (1–45). It remains unclear whether appropriate ethics provisions, in line with Frontiers Policies and Publication Ethics policies were adhered to.
The authors and a representative of Aix-Marseille Université Ethics Committee were not able to address all our concerns. As such, this article is retracted.
This retraction was approved by the Chief Executive Editor of Frontiers.
The authors do not agree with this retraction.
Correction note
This article has been corrected with minor changes. These changes do not impact the scientific content of the article.
References
1. Fall NS, Lo CI, Fournier PE, Sokhna C, Raoult D, Fenollar F, et al. Arcanobacterium ihumii sp. nov, Varibaculum vaginae sp nov and Tessaracoccus timonensis sp nov, isolated from vaginal swabs from healthy Senegalese women. New Microbes New Infect. (2019) 31:100585. doi: 10.1016/j.nmni.2019.100585
2. Bellali S, Bou Khalil J, Fontanini A, Raoult D, Lagier JC. A new protectant medium preserving bacterial viability after freeze drying. Microbiol Res. (2020) 236:126454. doi: 10.1016/j.micres.2020.126454
3. Afouda P, Hocquart M, Pham TPT, Kuete E, Ngom II, Dione N, et al. Alcohol pretreatment of stools effect on culturomics. Sci Rep. (2020) 10:5190. doi: 10.1038/s41598-020-62068-x
4. Bellali S, Lagier JC, Raoult D, Bou Khalil J. Among live and dead bacteria, the optimization of sample collection and processing remains essential in recovering gut microbiota components. Front Microbiol. (2019) 10:1606. doi: 10.3389/fmicb.2019.01606
5. Takakura T, Francis R, Anani H, Bilen M, Raoult D, Bou Khalil JY. Anaerosphaera massiliensis sp. nov, a new bacterium isolated from the stool of a 39-year-old pygmy. New Microbes New Infect. (2020) 33:100633. doi: 10.1016/j.nmni.2019.100633
6. Mekhalif F, Tidjani Alou M, Zgheib R, Lo CI, Fournier PE, Raoult D, et al. Brachybacterium massiliense sp. nov, a new bacterium isolated from stool from a healthy Senegalese child. New Microbes New Infect. (2019) 31:100588. doi: 10.1016/j.nmni.2019.100588
7. Kuete E, Mbogning Fonkou MD, Mekhalif F, Anani H, Baudoin JP, Raoult D, et al. Brachybacterium timonense sp. nov, a new bacterium isolated from human sputum. New Microbes New Infect. (2019) 31:100568. doi: 10.1016/j.nmni.2019.100568
8. Yimagou EK, Tall ML, Baudoin JP, Raoult D, Bou Khalil JY. Clostridium transplantifaecale sp. nov, a new bacterium isolated from patient with recurrent Clostridium difficile infection. New Microbes New Infect. (2019) 32:100598. doi: 10.1016/j.nmni.2019.100598
9. Dione N, Ngom II, Valles C, Cadoret F, Fournier PE, Raoult D, et al. ‘Collinsella provencensis' sp. nov, ‘Parabacteroides bouchesdurhonensis' sp nov and ‘Sutterella seckii,' sp nov, three new bacterial species identified from human gut microbiota. New Microbes New Infect. (2018) 23:44–7. doi: 10.1016/j.nmni.2018.02.003
10. Guindo CO, Terrer E, Chabrière E, Aboudharam G, Drancourt M, Grine G. Culture of salivary methanogens assisted by chemically produced hydrogen. Anaerobe. (2020) 61:102128. doi: 10.1016/j.anaerobe.2019.102128
11. Dubourg G, Morand A, Mekhalif F, Godefroy R, Corthier A, Yacouba A, et al. Deciphering the urinary microbiota repertoire by culturomics reveals mostly anaerobic bacteria from the gut. Front Microbiol. (2020) 11:513305. doi: 10.3389/fmicb.2020.513305
12. Afouda P, Traore SI, Dione N, Andrieu C, Tomei E, Richez M, et al. Description and genomic characterization of Massiliimalia massiliensis gen. nov, sp nov, and Massiliimalia timonensis gen nov, sp nov, two new members of the family Ruminococcaceae isolated from the human gut. Antonie Van Leeuwenhoek. (2019) 112:905–18. doi: 10.1007/s10482-018-01223-x
13. Senghor B, Seck EH, Khelaifia S, Bassène H, Sokhna C, Fournier PE, et al. Description of ‘Bacillus dakarensis' sp. nov, ‘Bacillus sinesaloumensis' sp nov, ‘Gracilibacillus timonensis' sp nov, ‘Halobacillus massiliensis' sp nov, ‘Lentibacillus massiliensis' sp nov, ‘Oceanobacillus senegalensis' sp nov, ‘Oceanobacillus timonensis' sp nov, ‘Virgibacillus dakarensis' sp nov and ‘Virgibacillus marseillensis' sp nov, nine halophilic new species isolated from human stool. New Microbes New Infect. (2017) 17:45–51. doi: 10.1016/j.nmni.2017.01.010
14. Ngom II, Hasni I, Senghor B, Lo CI, Armstrong N, Sokhna C, et al. Description of Gracilibacillus phocaeensis sp. nov, a new halophilic bacterium isolated from Senegalian human stool. New Microbes New Infect. (2020) 38:100799. doi: 10.1016/j.nmni.2020.100799
15. Hassani Y, Brégeon F, Aboudharam G, Drancourt M, Grine G. Detection of Methanobrevobacter smithii and Methanobrevibacter oralis in lower respiratory tract microbiota. Microorganisms. (2020) 8:1866. doi: 10.3390/microorganisms8121866
16. Takakura T, Francis R, Anani H, Naud S, Raoult D, Bou Khalil JY. Dorea phocaeensis sp. nov, a new bacterium isolated from the stool of a healthy 29-year-old male. New Microbes New Infect. (2019) 32:100600. doi: 10.1016/j.nmni.2019.100600
17. Diakite A, Dubourg G, Dione N, Afouda P, Bellali S, Ngom II, et al. Extensive culturomics of 8 healthy samples enhances metagenomics efficiency. PLoS ONE. (2019) 14:e0223543. doi: 10.1371/journal.pone.0223543
18. Naud S, Hasni I, Bellali S, Kieu HT, Lo CI, Anani H, et al. Genomic and phenotypic description of Streptococcus resistens sp. nov., Streptococcus buccae sp. nov. and Streptococcus mediterraneus sp. nov., three new members of the Streptococcus genus isolated from the human oral cavity. Res Sq. (2021). Available online at: https://www.researchsquare.com/article/rs-238204/v1 doi: 10.21203/rs.3.rs-238204/v1 (Accessed June 6, 2025).
19. Senghor B, Khelaifia S, Bassène H, Seck EH, Fournier PE, Sokhna C, et al. ‘Gracilibacillus phocaeensis' sp. nov, ‘Sediminibacillus massiliensis' sp nov and ‘Virgibacillus ndiopensis' sp nov, three halophilic species isolated from salty human stools by culturomics. New Microbes New Infect. (2017) 20:51–4. doi: 10.1016/j.nmni.2017.08.006
20. Fall NS, Raoult D, Sokhna C, Lagier JC. ‘Helcococcus massiliensis' sp. nov, a new bacterial species isolated from the vaginal sample of a woman with bacterial vaginosis living in Dielmo, Senegal. New Microbes New Infect. (2018) 25:27–9. doi: 10.1016/j.nmni.2018.06.002
21. Traore SI, Khelaifia S, Armstrong N, Lagier JC, Raoult D. Isolation and culture of Methanobrevibacter smithii by co-culture with hydrogen-producing bacteria on agar plates. Clin Microbiol Infect. (2019) 25:1561.e1–5. doi: 10.1016/j.cmi.2019.04.008
22. Hamad I, Abdallah RA, Ravaux I, Mokhtari S, Tissot-Dupont H, Michelle C, et al. Metabarcoding analysis of eukaryotic microbiota in the gut of HIV-infected patients. PLoS ONE. (2018) 13:e0191913. doi: 10.1371/journal.pone.0191913
23. Amrane S, Hocquart M, Afouda P, Kuete E, Pham TPT, Dione N, et al. Metagenomic and culturomic analysis of gut microbiota dysbiosis during Clostridium difficile infection. Sci Rep. (2019) 9:12807. doi: 10.1038/s41598-019-49189-8
24. Mbogning Fonkou MD, Bilen M, Gouba N, Khelaifia S, Cadoret F, Nguyen TT, et al. Non-contiguous finished genome sequencing and description of Enterococcus timonensis sp. nov isolated from human sputum. New Microbes New Infect. (2019) 29:100532. doi: 10.1016/j.nmni.2019.100532
25. Andrieu C, Mailhe M, Ricaboni D, Fonkou MDM, Bilen M, Cadoret F, et al. Noncontiguous finished genome sequences and description of Bacteroides mediterraneensis sp. nov, Bacteroides ihuae sp nov, Bacteroides togonis sp nov, Bacteroides ndongoniae sp nov, Bacteroides ilei sp nov and Bacteroides congonensis sp nov identified by culturomics. New Microbes New Infect. (2018) 26:73–88. doi: 10.1016/j.nmni.2018.06.006
26. Senghor B, Bassène H, Khelaifia S, Robert C, Fournier PE, Ruimy R, et al. Oceanobacillus timonensis sp. nov and Oceanobacillus senegalensis sp nov, two new moderately halophilic, gram-stain positive bacteria isolated from stools sample of healthy young Senegalese. Antonie Van Leeuwenhoek. (2019) 112:785–96. doi: 10.1007/s10482-018-01212-0
27. Diakite A, Dubourg G, Dione N, Afouda P, Bellali S, Ngom II, et al. Optimization and standardization of the culturomics technique for human microbiome exploration. Sci Rep. (2020) 10:9674. doi: 10.1038/s41598-020-66738-8
28. Maaloum M, Lo CI, Ndongo S, Meng MM, Saile R, Alibar S, et al. Ottowia massiliensis sp. nov., a new bacterium isolated from a fresh, healthy human fecal sample. FEMS Microbiol Lett. (2022) 369:fnac086. doi: 10.1093/femsle/fnac086
29. Yimagou EK, Dione N, Ngom II, Tall ML, Baudoin JP, Raoult D, et al. Parabacteroides bouchesdurhonensis sp. nov, a new bacterium isolated from the stool of a healthy adult. New Microbes New Infect. (2020) 34:100639. doi: 10.1016/j.nmni.2019.100639
30. Belkacemi S, Alou MT, Million M, Levasseur A, Khelaifia S, Raoult D. Prevalence of treponema species in the gut microbiome is linked to Bifidobacterium sp. and Bacteroides sp. Res Sq. (2020). Available online at: https://www.researchsquare.com/article/rs-117420/v1 doi: 10.21203/rs.3.rs-117420/v1 (Accessed June 6, 2025).
31. Anani H, Guilhot E, Andrieu C, Fontanini A, Raoult D, Fournier PE. Prevotella ihumii sp. nov, a new bacterium isolated from a stool specimen of a healthy woman. New Microbes New Infect. (2019) 32:100607. doi: 10.1016/j.nmni.2019.100607
32. Kuete Yimagou E, Mekhalif F, Lamine Tall M, Baudoin JP, Raoult D, Bou Khalil JY. Prevotella marseillensis sp. nov, a new bacterium isolated from a patient with recurrent Clostridium difficile infection. New Microbes New Infect. (2019) 32:100606. doi: 10.1016/j.nmni.2019.100606
33. Maaloum M, Afouda P, Lo CI, Dubourg G, Nguyen TT, Levasseur A, et al. Prevotella merdae sp. nov., a new bacterial species isolated from human faeces. FEMS Microbiol Lett. (2022) 369:fnac066. doi: 10.1093/femsle/fnac066
34. Ndongo S, Armstrong N, Raoult D, Fournier PE. Reclassification of eight Akkermansia muciniphila strains and description of Akkermansia massiliensis sp. nov and Candidatus Akkermansia timonensis, isolated from human feces Sci Rep. (2022) 12:21747. doi: 10.1038/s41598-022-25873-0
35. Hamad I, Ranque S, Azhar EI, Yasir M, Jiman-Fatani AA, Tissot-Dupont H, et al. RETRACTED ARTICLE: culturomics and amplicon-based metagenomic approaches for the study of fungal population in human gut microbiota. Sci Rep. (2017) 7:16788. doi: 10.1038/s41598-017-17132-4
36. Belkacemi S, Mazel A, Tardivo D, Tavitian P, Stephan G, Bianca G, et al. RETRACTED ARTICLE: peri-implantitis-associated methanogens: a preliminary report. Sci Rep. (2018) 8:9447. doi: 10.1038/s41598-018-27862-8
37. Grine G, Terrer E, Boualam MA, Aboudharam G, Chaudet H, Ruimy R, et al. RETRACTED ARTICLE: tobacco-smoking-related prevalence of methanogens in the oral fluid microbiota. Sci Rep. (2018) 8:9197. doi: 10.1038/s41598-018-27372-7
38. Amrane S, Bachar D, Lagier JC, Raoult D. RETRACTED: Clostridium scindens is present in the gut microbiota during Clostridium difficile infection: a metagenomic and culturomic analysis. J Clin Microbiol. (2018) 56:10.1128/jcm.01663-17. doi: 10.1128/JCM.01663-17
39. Bellali S, Lagier JC, Million M, Anani H, Haddad G, Francis R, et al. Running after ghosts: are dead bacteria the dark matter of the human gut microbiota? Gut Microbes. (2021) 13:1897208. doi: 10.1080/19490976.2021.1897208
40. Senghor B, Bassène H, Khelaifia S, Robert C, Fournier PE, Ruimy R, et al. Sediminibacillus massiliensis sp. nov, a moderately halophilic, gram-positive bacterium isolated from a stool sample of a young Senegalese man. Antonie Van Leeuwenhoek. (2018) 111:1225–36. doi: 10.1007/s10482-018-1032-x
41. Kuete E, Mbogning Fonkou MD, Mekhalif F, Anani H, Baudoin JP, Raoult D, et al. Selenomonas felix sp. nov, a new bacterium isolated from human sputum. New Microbes New Infect. (2019) 31:100567. doi: 10.1016/j.nmni.2019.100567
42. Benabdelkader S, Andreani J, Gillet A, Terrer E, Pignoly M, Chaudet H, et al. Specific clones of Trichomonas tenax are associated with periodontitis. PLoS ONE. (2019) 14:e0213338. doi: 10.1371/journal.pone.0213338
43. Hocquart M, Pham T, Kuete E, Tomei E, Lagier JC, Raoult D. Successful fecal microbiota transplantation in a patient suffering from irritable bowel syndrome and recurrent urinary tract infections. Open Forum Infect Dis. (2019) 6:ofz398. doi: 10.1093/ofid/ofz398
44. Yimagou EK, Anani H, Yacouba A, Hasni I, Baudoin JP, Raoult D, et al. Urinicoccus massiliensis gen. nov, sp nov, a new bacterium isolated from a human urine sample from a 7-year-old boy hospitalized for dental care. New Microbes New Infect. (2019) 32:100615. doi: 10.1016/j.nmni.2019.100615
Citation: Frontiers Editorial Office (2025) Retraction: Tobacco smoking affects the salivary gram-positive bacterial population. Front. Public Health 13:1654640. doi: 10.3389/fpubh.2025.1654640
Received: 26 June 2025; Accepted: 27 June 2025;
Published: 01 July 2025; Corrected: 07 July 2025.
Approved by:
Aleksandra Barac, University of Belgrade, SerbiaCopyright © 2025 Frontiers Editorial Office. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Frontiers Editorial Office, cmVzZWFyY2guaW50ZWdyaXR5QGZyb250aWVyc2luLm9yZw==