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Correction ARTICLE

Front. Cell. Infect. Microbiol., 29 October 2020 | https://doi.org/10.3389/fcimb.2020.593413

Corrigendum: Identification of Novel Laminin- and Fibronectin-Binding Proteins by Far-Western Blot: Capturing the Adhesins of Streptococcus suis Type 2

Quan Li, Hanze Liu, Dechao Du, Yanfei Yu, Caifeng Ma, Fangfang Jiao, Huochun Yao, Chengping Lu and Wei Zhang*
  • Key Lab of Animal Bacteriology, Ministry of Agriculture, College of Veterinary Medicine, China OIE Reference Lab for Swine Streptococcosis, Nanjing Agricultural University, Nanjing, China

A Corrigendum on
Identification of Novel Laminin- and Fibronectin-Binding Proteins by Far-Western Blot: Capturing the Adhesins of Streptococcus suis Type 2

By Li, Q., Liu, H. Z., Du, D. C., Yu, Y. F., Ma, C. F., Jiao, F. F., et al. (2015). Front Cell Infect Microbiol. 5:82. doi: 10.3389/fcimb.2015.00082

In the original article, there was a mistake in the legend for Figures 1 and 2 as published. There are some creases and non-specific stains appear in the original Figures 1F, H and 2C. In order to improve the presentation quality of the figures, we modified the creases and non-specific stains outside of the results. These modifications do not affect the results of the article. This clarification needs to be added to the figure legends of these figures. The correct legends appears below.

FIGURE 1
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Figure 1 2-DE gels and Far-Western blot identification of LN- and FN-binding proteins of SS2 CW and EC. The CW and EC proteins were separated in the first dimension by IEF in the pI range of 4–7 and by 12% SDS-PAGE in the second dimension, and then the 2-DE gels transferred to PVDF and incubated with LN or LN. Arrows indicate potential LN- or FN-binding proteins recognized with goat anti-rabbit IgG antibody. (A) 2-DE gel of SS2 CW proteins. (B) Far-Western blot of CW proteins incubated with LN (LN-CW). (C) 2-DE gel of SS2 EC proteins. (D) Far-Western blot of EC proteins incubated with LN (LN-EC). (E) 2-DE gel of SS2 CW proteins. (F) Far-Western blot of CW proteins incubated with FN (FN-CW). (G) 2-DE gel of SS2 EC proteins. (H) Far-Western blot of EC proteins incubated with FN (FN-EC). In order to improve the presentation quality of (F, H), we modified the creases and non-specific stains outside of the results. These modifications do not affect the results of the article.

FIGURE 2
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Figure 2 Determine the binding of the recombinant proteins to LN and FN by Far-Western blot. Coomassie G-250-stained gel (A), Western blot analysis (B), and Far-Western blot analysis (C, D) of the SS2 recombinant proteins. Recombinant proteins were separated by 12% SDS-PAGE, then transferred to PVDF membrane and incubated with human LN or LN. Bound LN or FN was detected with goat anti-rabbit IgG antibody. The Western blot (B) was probed with his tag monoclonal antibody (Boster). In order to improve the presentation quality of (C), we modified the creases outside of the results. These modifications do not affect the results of the article.

In the original article, there was a mistake in Figure 4 as published. Each sample hole was photographed twice or more for the same or different field of view. Due to the similarity in the fluorescence intensity of LDH protein and IMPDH protein, we made a mistake while selecting the pictures among a lot of pictures. Another LDH picture from the same hole with different views was incorrectly named IMPDH. The corrected Figure 4 appears below.

FIGURE 4
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Figure 4 Adherence of recombinant proteins to Hep-2 cells confirmed by an indirect immunofluorescence assay.

The authors apologize for these errors and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

Keywords: Streptococcus suis serotype 2, interactions, surface proteins, LN- binding proteins, FN-binding proteins

Citation: Li Q, Liu H, Du D, Yu Y, Ma C, Jiao F, Yao H, Lu C and Zhang W (2020) Corrigendum: Identification of Novel Laminin- and Fibronectin-Binding Proteins by Far-Western Blot: Capturing the Adhesins of Streptococcus suis Type 2. Front. Cell. Infect. Microbiol. 10:593413. doi: 10.3389/fcimb.2020.593413

Received: 10 August 2020; Accepted: 12 October 2020;
Published: 29 October 2020.

Edited and reviewed by: Thomas Rudel, Julius Maximilian University of Würzburg, Germany

Copyright © 2020 Li, Liu, Du, Yu, Ma, Jiao, Yao, Lu and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Wei Zhang, vszw@njau.edu.cn