Error in Figure/Table
In the original article, there was a mistake in Table 3 as published. The data under columns for the header “Large segment” and the header “Small segment” were mistakenly switched prior to submission. The corrected Table 3 appears below.
Table 3
| Sample Source | PCR Cycle | Pool | Large Segment | Medium Segment | Small Segment | |
|---|---|---|---|---|---|---|
| % Genome Coverage | ||||||
| Supernatant | 1x | 1 | 100% | 96% | 98% | |
| 3 | 100% | 97% | 96% | |||
| 7x | 1 | 100% | 96% | 99% | ||
| 3 | 0% | 98% | 99% | |||
| 15x | 1 | 0% | 83% | 99% | ||
| 3 | 0% | 82% | 99% | |||
| 20x | 1 | 0% | 82% | 99% | ||
| 3 | 0% | 96% | 98% | |||
| Cells | 1x | 1 | 0% | 0% | 0% | |
| 3 | 0% | 0% | 0% | |||
| 7x | 1 | 0% | 0% | 0% | ||
| 3 | 0% | 0% | 0% | |||
| 15x | 1 | 0% | 0% | 0% | ||
| 3 | 0% | 82% | 97% | |||
| 20x | 1 | 0% | 0% | 0% | ||
| 3 | 0% | 82% | 96% | |||
Percent genome coverage of ANDV Illumina MiSeq run using a 400x minimum coverage depth.
For those samples stated as “3 pool”, the sequencing strategy ( Figure 1D) used overlapping amplicons for each segment, however primers were placed into one of three disjointed primer pools. The multiplexed amplicons were purified and normalized before the 3 pools were mixed for preparation of Nextera XT libraries. In contrast, for those samples stated as “1 pool”, all amplicons were synthesized in one reaction in Figure 1B (one pool) and purified for preparation of Nextera XT libraries.
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
In the original article, there was an error. This error included a statement which described the incorrect Table 3 in the Results section.
A correction has been made to Results, Two-Step Amplification of Full-Length Sequences of S, M, and L Segment vRNA of ANDV Using MiSeq From ANDV-Infected Vero E6 Supernatant or ANDV-Infected Vero E6 Cells, Paragraph number 5:
We used a 400x depth of coverage threshold to assess ANDV genome coverage (Table 3). The supernatant samples which gave the highest genome coverage across all segments was the 1x PCR and 7x PCR. The samples from infected cells showed good coverage at 15x and 20x PCR cycles although this was only true for the S and M segments as the L segment provided no coverage at this depth.
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
Publisher’s Note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Summary
Keywords
hantavirus, Orthohantavirus, NGS, next generation sequencing, MinION, illumina MiSeq, single nucleotide polymorphism, SNP
Citation
Taylor MK, Williams EP, Wongsurawat T, Jenjaroenpun P, Nookaew I and Jonsson CB (2022) Corrigendum: Amplicon-Based, Next-Generation Sequencing Approaches to Characterize Single Nucleotide Polymorphisms of Orthohantavirus Species. Front. Cell. Infect. Microbiol. 11:804914. doi: 10.3389/fcimb.2021.804914
Received
29 October 2021
Accepted
15 November 2021
Published
24 January 2022
Volume
11 - 2021
Edited and reviewed by
Connie S. Schmaljohn, Integrated Research Facility (NIAID), United States
Updates
Copyright
© 2022 Taylor, Williams, Wongsurawat, Jenjaroenpun, Nookaew and Jonsson.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Colleen B. Jonsson, cjonsson@uthsc.edu
This article was submitted to Virus and Host, a section of the journal Frontiers in Cellular and Infection Microbiology
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.