%A Chen,Pengfei
%A Zhou,Liying
%A Chen,Jiying
%A Lu,Ying
%A Cao,Chaoxia
%A Lv,Shuangli
%A Wei,Zhihong
%A Wang,Liping
%A Chen,Jiao
%A Hu,Xinglin
%A Wu,Zijing
%A Zhou,Xiaohua
%A Su,Danna
%A Deng,Xuefeng
%A Zeng,Changchun
%A Wang,Huiyun
%A Pu,Zuhui
%A Diao,Ruiying
%A Mou,Lisha
%D 2021
%J Frontiers in Immunology
%C
%F
%G English
%K unexplained recurrent pregnancy loss,Human decidua,immune heterogeneity,single-cell RNA sequencing,decidual natural killer cell,ScRNA,the immune atlas
%Q
%R 10.3389/fimmu.2021.689019
%W
%L
%M
%P
%7
%8 2021-June-07
%9 Original Research
%+ Zuhui Pu,Department of Radiology, First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital,China,lishamou@email.szu.edu.cn
%+ Ruiying Diao,Centre of Reproductive Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital,China,lishamou@email.szu.edu.cn
%+ Lisha Mou,Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital,China,lishamou@email.szu.edu.cn
%#
%! Unexplained recurrent pregnancy loss
%*
%<
%T The Immune Atlas of Human Deciduas With Unexplained Recurrent Pregnancy Loss
%U https://www.frontiersin.org/articles/10.3389/fimmu.2021.689019
%V 12
%0 JOURNAL ARTICLE
%@ 1664-3224
%X Recurrent pregnancy loss (RPL) is a common fertility problem that affects 1%-2% of couples all over the world. Despite exciting discoveries regarding the important roles of the decidual natural killer cell (dNK) and regulatory T cell in pregnancy, the immune heterogeneity in patients with unexplained recurrent pregnancy loss (URPL) remains elusive. Here, we profiled the transcriptomes of 13,953 CD45+ cells from three normal and three URPL deciduas. Based on our data, the cellular composition revealed three major populations of immune cells including dNK cell, T cell, and macrophage, and four minor populations including monocytes, dendritic cell (DC), mast cell, and B cell. Especially, we identified a subpopulation of CSF1+ CD59+ KIRs-expressing dNK cells in normal deciduas, while the proportion of this subpopulation was decreased in URPL deciduas. We also identified a small subpopulation of activated dDCs that were accumulated mainly in URPL deciduas. Furthermore, our data revealed that in decidua at early pregnancy, CD8+ T cells exhibited cytotoxic properties. The decidual macrophages expressed high levels of both M1 and M2 feature genes, which made them unique to the conventional M1/M2 classification. Our single-cell data revealed the immune heterogeneity in decidua and the potentially pathogenic immune variations in URPL.