Translocation of Phthalates From Food Packaging Materials Into Minced Beef

Introduction

Over the past decades, a vast number of studies related to the existence of phthalate compounds in packaging materials and food have been published (1–4). Phthalates (diesters of ortho phthalic acid) are organic chemicals that are commonly used as plasticizers to make plastic polymers softer and more flexible. Additionally, they are used to produce lacquers and printing inks as additives to improve the flexibility, surface adhesion, color, elasticity, and wrinkle resistance to manufacturing adhesives, solvents, waxes, pharmaceuticals, and cosmetics insecticides, and packaging from regenerated cellulose (5).

The presence of phthalates in food poses a significant concern regarding their impact on human health (6). Following exposure and uptake, phthalates are rapidly metabolized by hydrolysis followed by conjugation (7). The first phase, which occurred in the intestine and parenchyma and catalyzed by esterases and lipases, the diester phthalate is hydrolyzed into the monoester phthalate (8). Monoester phthalates are more bioactive than non-hydrolyzed diester phthalates, as shown in in vitro and in vivo studies. Short-branched phthalates are mainly excreted as monoester phthalates via urine. In contrast, long-branched compounds are further biotransformed through hydroxylation and oxidation and then excreted in feces and urine as phase II conjugated compounds (9). Phthalates were also found in breast milk and amniotic fluid (10).

It was reported that exposure to phthalates and their metabolites might inadvertently be disrupting the endocrine system, act as carcinogens, and negatively impact the reproductive system (11). Some phthalates may have anti-androgenic and cause reproductive and developmental toxicity in animals (12). Researches demonstrated that phthalates could adversely affect the male reproductive system, causing asthma, rhinitis, and eczema in children, in addition to their impacts on metabolism and neurological development (13). Neurotoxicity, infertility, respiratory symptoms, epigenetic, immune and metabolic abnormalities are other endpoints of target organ toxicity caused by phthalates (14).

People are mainly exposed to phthalates from food, water, air, and consumer products, including building materials, household furnishings, clothing, cosmetics, pharmaceuticals, nutritional supplements, medical devices, dentures, children's toys, glow sticks, modeling clay, food packaging, automobiles, lubricants, waxes, cleaning materials, and insecticides (15). The routes of phthalate exposure ranked by their importance include food intake, dust ingestion, and indoor air inhalation (16). Mechanical stress or high temperature can cause chemical migration from packaging materials to food or drink (17). Phthalates are readily leached from products and released to the environment because of their weak bonds with bridging substrate (11).

In addition to the direct effect of phthalates through food items, their migration to the soil, groundwater, and dust can pose a significant risk to human health and/or sensitive environmental receptors. Plastics account for 25% of municipal solid waste (18). The most rational way for treating plastic is separate collection and recycling as it gives the possibilities for environmentally friendly management of every municipal solid waste stream (19). Even in this case, plastic materials should be handled, treated, and disposed of safely (20). In the case of landfilling of plastic materials, the impact of phthalates on environmental quality is associated with certain long-term related soil and groundwater contamination problems (21).

European Union has set limits and regulations for the content of chemical compounds in packaging. European Food Safety Authority has specified tolerable daily intakes (TDIs) of 0.01 mg × kg⁻¹ bw for di-n-butyl phthalate (DnBP), 0.05 mg × kg⁻¹ bw for di(2-ethylhexyl) phthalate (DEHP), 0.50 mg × kg⁻¹ bw for benzylbutyl phthalate (BBP), and a group TDI of 0.15 mg × kg⁻¹ bw for diisodecyl phthalate (DiDP) and diisononyl phthalate (DiNP) (22–26). Russia has also established the rules governing the packaging components migration tests using simulators of food (Technical Regulations CU TR 005/2011 on the safety of the packaging). However, it is still inadequately controlled. Although there were many studies on phthalates in packaging materials and food in Europe and America (27–31), Russia lacks information. Hence, this study aimed to evaluate the migration of phthalates from various common packaging materials to beef sold in the Russian meat market.

Materials and Methods

Standards, Solvents, and Chemicals

Phthalate standards, including dimethyl terephthalate (DMTP), di-n-butyl phthalate (DnBP), and diisooctyl phthalate (DiOP), were procured from ChromLab Ltd. (Lyubertsy, Russia). LC grade or glass-distilled solvents were used. Dichloromethane and hexane were from Merck (Darmstadt, Germany). HPLC gradient grade methanol was from J.T.Baker (Deventer, Netherlands). Water is produced using Milli-Q Type 1 Ultrapure Water Systems (Millipore, Burlington, Massachusetts, United States).

Standard stock solution for each analyte (DMTP, DnBP, and DiOP) was prepared in methanol at a 1 mg·ml⁻¹ concentration. These solutions were further diluted in methanol, yielding various concentrations (0.005, 0.01, 0.1, and 0.5 mg·ml⁻¹) for constructing the calibration curves. All working solutions are prepared freshly before use.

During all manipulations, contact with any plastic material was not allowed. All glassware and accessories were rinsed with methanol, acetone, and n-hexane, immediately before and after use.

Sample Collection

Beef striploin (50 samples) and packaging materials (35 samples) were secured from various shopping areas in Saint-Petersburg and Leningrad region, Northwestern Federal District, Russia, between November 2020 and May 2021. Samples of beef striploin were selected with an average fat content of about 20%. It should be noted that the distribution networks of the Northwestern Federal District and the range of goods are common or very similar for beef and packaging materials compared to most regions of Russia.

Farm beef samples (40 samples) were obtained from striploin cuts weighing 4.5–5 kg each from three carcasses. These three striploin cuts were delivered to the laboratory in glass containers. In the laboratory, the cuts were carved into appropriate pieces and analyzed, minced and/or packed in five samples for each type of processing. Each sample was packaged and sealed in selected packaging material, as shown in Table 1. Each packaging sample was taken from a single roll or unit of packaging material. Samples of commercially packaged beef were purchased in quantities of five for each of the two types. The pH of the farm fresh beef was 5.7 ± 0.1 (24 h), increased to 6.1 ± 0.1 (120 h) after slaughter. The pH of the chilled commercially packaged beef samples was 6.0 ± 0.2. The water content of farm fresh beef, commercially packaged beef sample 1, and commercially packaged beef sample two were 64.5 ± 0.8, 65.7 ± 0.5, and 65.2 ± 0.7, whereas the fat contents were 16 ± 1, 17.3 ± 0.8, and 18.6 ± 0.7, respectively. Except for commercially packaged and minced beef, all samples were sliced at 10 ± 2 mm thickness. Some samples were subjected to microwave heat treatment for 10 min at a power of 900 W. Each sample was homogenized in a stainless-steel laboratory blender Grindomix GM200 (Retsch GmbH, Haan, Germany) before analysis. The blender was thoroughly cleaned after processing each sample to avoid cross-contamination.

TABLE 1

Table 1. Food samples, packaging types, and conditions.

Extraction

To ensure low backgrounds from phthalate contamination, all glassware was rinsed with glass-distilled acetone immediately before use and only glass-distilled or HPLC grade solvents were utilized. At all stages of analysis, contact between food samples and materials other than glass, PTFE, or stainless steel was avoided. Extraction using organic solvents is widely used for phthalates analysis in solid matrix samples, such as food-packaging materials and foods (32). An average sample with a mass of about 10 g was taken from each packaged striploin or minced beef. Average meat samples were blended with the same mass of sodium sulfate and 50 ml of dichloromethane. The supernatant was filtered and removed by rotary evaporation. The weight of extracted fat was determined. Plasticizers were then isolated from aliquots of the lipid material with hexane, then analyzed by gas chromatography coupled with mass spectrometry (GC-MS).

Gas Chromatography

Analysis was carried out by GC-2010 Plus gas chromatograph (Shimadzu, Kyoto, Japan) linked with gas chromatograph-mass spectrometer GCMS-TQ8040. The separation was performed on a cross bonded a 30 m × 0.25 mm I.D. Rxi-5Sil MS column (Restek, Bellefonte, Pennsylvania, USA) coated with 5% diphenyl 95% dimethylpolysiloxane (film thickness 0.25 μm). The oven temperature was programmed from 50°C (holding time 5 min) to 300°C at 5°C min⁻¹. One μl was injected in the injection mode (split ratio 1:10). Helium was used as the carrier gas (1.1 ml min−1), and the injection temperature was set at 250°C. The electron-impact ionization mass spectrometer was operated as follows: ionization voltage, 70 eV; interface and ion source temperatures were 275°C and 200°C, respectively; scan mode, mass range 40.0–500.0; solvent cut time 2 min. Plasticizer identification was performed by comparing mass spectra with those in the mass spectral library (NIST, Gaithersburg, USA). The phthalates were detected using a SCAN mode (Figure 1).

FIGURE 1

Figure 1. GC–MS chromatograms of standard methanol solutions of DMTP, DnBP, and DiOP in SCAN mode.

A base peak and a qualifier ion were chosen by the intensity of signals for each phthalate (Table 2). The dominant product ion 149 m/z was used to quantify all phthalates, except for DMTP. The ion 163 m/z was used for DMTP quantification. The qualifier to target the ion ratio of each phthalate had to be <20% of the same in a standard solution for positive identification. The retention times on the Rxi-5Sil MS column, similarity range and the characteristic m/z values for these compounds are summarized in Table 2.

TABLE 2

Table 2. Conditions for GC-MS analysis of phthalates.

Quantification was based on comparing the areas of identified peaks with calibration curves in GC-MS Solution software (Shimadzu, Kyoto, Japan). In the studied concentration range, the calibration curves fitted a linear mode (R²> 0.99). The State Pharmacopeia of the Russian Federation (XIV edition, 2018) indicates that in most cases, linear models are used if they meet the condition R ≥ 0.99. In our case, for the least-squares model, this corresponds to R² ≥ 0.9801. Also, R²> 0.99 corresponds to the previously developed methods for determining phthalates in meat (33). Method performance is shown in Table 3.

TABLE 3

Table 3. Method performance for GC-MS analysis of phthalates.

To minimize the risk of contamination, each analytical batch was composed of several samples of blank solvent, standard calibration solutions, a reference, and several experimental samples (five replicates at the most). The whole procedure was carried out using minimum solvents and glassware and as fast and straightforward as possible. Mathematical statistics methods were used for data processing at a theoretical frequency of 0.95. To determine the significant differences between the values, a two-sample t-test was used for each pair of means.

Results and Discussion

Fasano et al. (11) investigated the migration of phthalates from a common form of food packaging. They concluded that simulants may be helpful for identification and comparing legislated maximum concentrations of compounds migrating from containers. However, this is not always adequate to real conditions, and lately, migration experiments should be performed directly in food. The use of mass spectrometry makes it possible to evaluate the migration of phthalates not only in simple phantom mixtures but also in real objects with a complex matrix (34). So, we used the meat samples as research objects in our study.

Figure 2 shows the typical chromatogram of chilled minced farm beef extract previously stored in a vacuum package. Selected conditions gave an excellent resolution of phthalates peaks, and the matrix did not interfere with the identification. This proves that phthalates could be adequately identified and quantified in all investigated samples.

FIGURE 2

Figure 2. GC–MS chromatogram of an extract of chilled minced farm beef in a vacuum package.

The food's specific surface area, moisture, fat content, and thermal (microwave) treating impact the degree and rate of migration of harmful substances (11). That is why cut and minced samples were prepared for the research. The effect of temperature on migration was investigated for storage at 2 and −18°C, as well as after heat treatment using microwave radiation. The results of various beef samples are presented in Table 4.

TABLE 4

Table 4. Levels of phthalates in beef samples μg·kg⁻¹ (mean ± sd).

In general, the obtained values of phthalate concentrations vary wildly, even for the same type of plastic. This might be attributed to the fact that each plastic material uses different plasticizers and additives.

We have found phthalates in the farm-fresh beef that has been collected directly after the slaughter at the farm with no contact with any packaging materials. This finding suggests phthalates contamination of farm fodder or water, which necessitated further research.

The use of all packaging and processing options for farm fresh beef increased phthalate content compared to untreated and unpackaged samples. This indicates the migration of these xenobiotics from all types of the investigated packaging for almost any condition. The large contact area and the presence of more fat on the surface of the minced meat resulted in significantly higher phthalate migration (DnBP: 378.5 μg·kg⁻¹, DiOP: 37 μg·kg⁻¹) than beef slices (DnBP: 88.2 μg·kg⁻¹, DiOP: 12.2 μg·kg⁻¹). Deserves attention, frozen storage resulted in nearly the same phthalate levels (DnBP: 89 μg·kg⁻¹, DiOP: 10 μg·kg⁻¹) as refrigerated storage (DnBP: 88.2 μg·kg⁻¹, DiOP: 12.2 μg·kg⁻¹). This is probably due to phthalates migration during freezing and thawing since it is evident that the migration of components at −18°C should be very slow. This requires further research. However, as a primary recommendation, more intensive freezing of packaged meat products and defrosting outside plastic packaging can be proposed.

DnBP showed the highest mean values for most samples ranging from 34.5 to 378.5 μg·kg⁻¹. DiOP displayed the lower mean values, ranging from LOD to 37 μg·kg⁻¹. Particular attention should be paid to the fact that according to regulatory documents in Russia, DnBP presence in food is not allowed. The European Union regulation EC No. 11/2011 also states that DnBP is not allowed in fat-containing food. DiOP contents correspond to Russian normative documents (CU TR 005/2011). Also, they correspond to the European Union regulation EC No. 10/2011, which established a limit of 60 mg·kg⁻¹ for DiOP in food products. Regarding phthalates regulation, the USA and the European Union have harmonized their directives and implemented a “threshold policy” (35). Thus, the conclusions drawn regarding the obtained DnBP and DiOP concentrations for the European Union are also relevant for the United States. Polyethylene terephthalate (PET) used in the study is usually made with the addition of DMTP, and it was expected to be found. However, DMTP could not be recovered and was not detected in any samples. Thus, the DMTP concentration was below the LOD in the studied samples. One of the possible reasons for this may be its less use in the production of plastic packaging since it is more used as solvent and fixative in fragrances, additive in cosmetics, medical devices, and household and personal care products (36).

Analysis of phthalates in foods sold on the Belgian market revealed DnBP in 14 out of 22 meat and meat products with concentrations of up to 15.0 μg·kg⁻¹(37). These concentrations in beef correlated with predicted by environmental food transfer model for organic contaminants in Europe (5). DnBP was determined in all the analyzed samples of spices used to cook the chicken meat in concentrations ranging from 3.47 to 29.3 μg·kg⁻¹ (38). The chicken meat samples roasted with spices in a plastic bag presented concentrations from 0.1 to 1.17 μg·kg⁻¹ for DnBP. DnBP was the most frequently detected ortho-phthalate in USA fast food samples (39). It was detected in 81% of all food items in median concentrations of 2.4–4.8 μg·kg⁻¹. A study of convenience meat products on the Chinese market (40) found a median DnBP content of 336 μg·kg⁻¹ for 48 samples. Also, it was found that the migration of the phthalates into foods increased with time and the temperature and their content in the convenience foods near the shelf-life was much higher than those which were just manufactured. This study confirms these trends concerning plastic packaging available on the Russian market. Research on phthalates in food packaged in various types of plastics worldwide suggests a need to carefully analyze their overall consumption and assess the associated health risks (33, 35).

Besides polymer packages, we found phthalates in foods stored in wrapping paper. The chilled farm beef samples wrapped in paper contained DnBP and DiOP 41 and 11 μg·kg⁻¹, respectively. The possible reason is the use of recycled cartons to produce paper. Using recycled fibers comes with new challenges, such as controlling potential packaging contamination by harmful chemicals introduced by using pre-and post-consumer waste. Numerous studies have revealed the migration of various contaminants, such as phthalates (41), diisopropyl naphthalenes, benzophenones and others (42). In a study conducted by USA Food and Drug Administration scientists reported detectable concentrations of DnBP in paper-based fast food packaging collected from restaurants (31). Also, this is understandable, as phthalate compounds are widely used additives for printing inks and lacquers in food contact materials.

Daily intake (DI) was determined and compared with its tolerable daily intake (TDI) value established by the European Food Safety Authority to assess the health risks of human exposure to phthalate residues in beef. The DnBP TDI value is 10 μg·kg⁻¹ body weight a day (23). Since no TDI values have been established for DiOP, the risk assessment was carried out only in relation to the detected DnBP. The DI (μg·kg⁻¹·day⁻¹) was determined by multiplying the daily beef consumption (kg·day⁻¹) by obtaining mean DnBP concentration (μg·kg⁻¹) and dividing by human body weight (kg). The daily beef consumption value of 27.4 g (0.0274 kg·day⁻¹) was obtained by dividing the stated annual beef consumption in Russia by 10 kg·year⁻¹ by 365 (43). An average body weight of 62 kg for adults was used as a reference value (44). The results are indicated in Table 5.

TABLE 5

Table 5. Estimated DI levels of DnBP residues in beef and their percentage of TDI level in adults.

Thus, the daily intake did not exceed TDI for all types of packaging and beef processing. At the same time, the largest percentage of TDI was determined for chilled minced beef in vacuum packaging. In the annual and, accordingly, the average daily beef consumption is 1.36% (10 kg per year of beef from 735.6 kg per year of all food products) (45). Thus, if we assume a similar level of phthalates translocation from plastic packaging to other food products, as well as previously unaccounted drinking water, the daily intake of DnBP and other phthalates may exceed TDI. Thus, it is necessary to reduce the amount of plastic packaging and reduce the content and migration of phthalates into food from it. Also, an obvious conclusion is a need for the global introduction of new types of packaging materials without phthalates. Bio-based resources can be a base for producing biopolymers in food packaging applications instead of conventional plastics traditionally produced from fossil fuels. Microorganisms can produce biopolymers through fermentative processes of different bioresources (e.g., polyhydroxyalkanoates), and biomass may be produced directly from different types of plants (starch, cellulose, pectin, zein, gluten) and animal materials (chitosan, gelatin, caseins) (46). Biopolymer production does not include phthalates as glyceryl monoesters, glycerol, fatty acids, and polyethylene glycol are used as common plasticizers (47).

Conclusions

The Technical Regulations TR 005/2011 on the safety of the packaging were adopted in 2011 in the Russian Federation. This document defined allowable migration amounts for DiOP of 2.00 mg·L⁻¹, and migration of DnBP into food products was not allowed. The examined food packages from the Russian market do not meet the requirements of this standard, as DnBP migrates to food products. Perhaps the manufacturers are still using the previous instruction from 1972, according to which the allowable migration amounts for DnBP was 0.25 mg·L⁻¹. The obtained results require discussion by all participants of the production processes and the use and control of food packaging materials.

The most alarming results obtained in this study are the presence of phthalates in beef produced on a farm in the Leningrad region and not subjected to packaging. Presumably, phthalates could enter animals' diet along with fodder, compound feed, or water. A full-scale study is needed to determine the pathways and sources of phthalates migration in the food chain. The implementation of separate collection and recycling of plastic materials in Russia may be a way to reduce the contamination of farm animals food chains with phthalates. Moreover, this should be carried out in such a way as to avoid cross-contamination of materials, for example, recycled paper. In addition, it is necessary to strive to reduce the use of plastic packaging. At present, both the encapsulated forms of food ingredients (48) and the edible food coatings that increase food shelf-life are being developed to improve product stability during storage (49).

Data Availability Statement

The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

Author Contributions

DB contributed to the study concept. DB and AE-A critically reviewed the article. DB, MB, IB, BA, and WL contributed to the design of the manuscript and figure preparation and edition. DB, MB, IB, BA, WL, and AE-A contributed to the acquisition and analysis of data and drafted the manuscript. All authors gave final approval for all aspects of the work, agreed to be fully accountable for ensuring the integrity and accuracy of the work, read, and approved the final manuscript.

Funding

This study was supported by the Russian Science Foundation (Grant No. 21-16-00124, https://rscf.ru/en/project/21-16-00124/).

Conflict of Interest

The reviewer (FO) declared a shared affiliation with one of the authors, (AE-A), to the handling editor at the time of review.

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher's Note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

References

1. Fromme H, Gruber L, Schlummer M, Wolz G, Boehmer S, Angerer J, et al. Intake of phthalates and di(2-ethylhexyl)adipate: results of the integrated exposure assessment survey based on duplicate diet samples and biomonitoring data. Environ Int. (2007) 33:1012–20. doi: 10.1016/j.envint.2007.05.006

PubMed Abstract | CrossRef Full Text | Google Scholar

2. Bopp K, Altkofer W. Survey on intake of certain phthalic acid esters in South Germany: comparative study of two boarding schools with conventional and organic nutrition. Dtsch Lebensm Rundsch. (2009) 105:35–8.

Google Scholar

3. COT Statement on Dietary Exposure to Phthalates–Data from the Total Diet Study (TDS). London, United Kingdom: Committee on Toxicity of Chemicals in Food, Consumer Products and the Environment.

4. Schecter A, Lorber M, Guo Y, Wu Q, Yun SH, Kannan K, et al. Phthalate concentrations and dietary exposure from food purchased in New York State. Environ Health Perspect. (2013) 121:473–9. doi: 10.1289/ehp.1206367

PubMed Abstract | CrossRef Full Text | Google Scholar

5. Fierens T, Servaes K, Van Holderbeke M, Geerts L, De Henauw S, Sioen I, et al. Analysis of phthalates in food products and packaging materials sold on the Belgian market. Food and Chem Toxicol. (2012) 50:2575–83. doi: 10.1016/j.fct.2012.04.029

PubMed Abstract | CrossRef Full Text | Google Scholar

6. Dodson RE, Nishioka M, Standley LJ, Perovich LJ, Brody JG, Rudel RA. Endocrine disruptors and asthma-associated chemicals in consumer products. Environ Health Perspect. (2012) 120:935–43. doi: 10.1289/ehp.1104052

PubMed Abstract | CrossRef Full Text | Google Scholar

7. Rusyn I, Peters JM, Cunningham ML. Modes of action and species-specific effects of di-(2-ethylhexyl)phthalate in the liver. Crit Rev Toxicol. (2006) 36:459–79. doi: 10.1080/10408440600779065

PubMed Abstract | CrossRef Full Text | Google Scholar

8. Calafat AM, Ye X, Silva MJ, Kuklenyik Z, Kuklenyik Z, Needham LL. Human exposure assessment to environmental chemicals using biomonitoring. Int J Androl. (2006) 29:166–71. doi: 10.1111/j.1365-2605.2005.00570.x

PubMed Abstract | CrossRef Full Text | Google Scholar

9. Frederiksen H, Skakkebæk NE, Andersson AM. Metabolism of phthalates in humans. Mol Nutr Food Res. (2007) 51:899–11. doi: 10.1002/mnfr.200600243

PubMed Abstract | CrossRef Full Text | Google Scholar

10. Ghisari M, Bonefeld-Jorgensen EC. Effects of plasticizers and their mixtures on estrogen receptor and thyroid hormone functions. Toxicol Let. (2009) 189:67–7. doi: 10.1016/j.toxlet.2009.05.004

PubMed Abstract | CrossRef Full Text | Google Scholar

11. Fasano E, Bono-Blay F, Cirillo T, Montuori P, Lacorte S. Migration of phthalates, alkylphenols, bisphenol A and di(2-ethylhexyl)adipate from food packaging. Food Control. (2012) 27:132–8. doi: 10.1016/j.foodcont.2012.03.005

CrossRef Full Text | Google Scholar

12. Silva MJ, Samandar E, Preau JJL, Reidy JA, Needham LL, Calafat AM. Quantification of 22 phthalate metabolites in human urine. J Chromatogr B. (2007) 860:106–12. doi: 10.1016/j.jchromb.2007.10.023

PubMed Abstract | CrossRef Full Text | Google Scholar

13. Meeker JD, Ferguson KK. Phthalates: human exposure and related health effects In: Schecter A, editor. Dioxins and Health: Including Other Persistent Organic Pollutants and Endocrine Disruptors. Hoboken: Wiley (2012). p. 415–43.

Google Scholar

14. Snedeker SM, Rudel RA, Just AC. Phthalates in Food Packaging, Consumer Products, and Indoor Environments. In: Snedeker SM, editor. Toxicants in Food Packaging and Household Plastics. London: Springer (2014). p. 31–59.

Google Scholar

15. Schettler T. Human exposure to phthalates via consumer products. Int J Androl. (2006) 29:134–9. doi: 10.1111/j.1365-2605.2005.00567.x

PubMed Abstract | CrossRef Full Text | Google Scholar

16. Wormuth M, Scheringer M, Vollenweider M, Hungerbuhler K. What are the sources of exposure to eight frequently used phthalic acid esters in Europeans? Risk Analysis. (2006) 26:803–24. doi: 10.1111/j.1539-6924.2006.00770.x

PubMed Abstract | CrossRef Full Text | Google Scholar

17. Rudel RA, Gray JM, Engel CL, Rawsthorne TW, Dodson RE, Ackerman JM, et al. Food packaging and bisphenol A and bis(2-ethyhexyl) phthalate exposure: findings from a dietary intervention. Environ Health Perspect. (2011) 119:914–20. doi: 10.1289/ehp.1003170

PubMed Abstract | CrossRef Full Text | Google Scholar

18. Kaspars K, Dzene KP, Blumberga D. Optimal strategies for municipal solid waste treatment–environmental and socio-economic criteria assessment. Energy Procedia. (2017) 128:512–19. doi: 10.1016/j.egypro.2017.09.071

CrossRef Full Text | Google Scholar

19. Barisa A, Dzene I, Rosa M, Dobraja K. Waste-to-biomethane concept application: a case study of Valmiera city in Latvia. Env Clim Tech. (2015) 15:48–58. doi: 10.1515/rtuect-2015-0005

CrossRef Full Text | Google Scholar

20. Aydin N. Review of Municipal Solid Waste Management in Turkey with a Particular Focus on Recycling of Plastics. Energy Procedia. (2017) 113:111–5. doi: 10.1016/j.egypro.2017.04.031

CrossRef Full Text | Google Scholar

21. Yogalakshmi KN, Singh S. Plastic waste: environmental hazards, its biodegradation, and challenges. In: Saxena G, Bharagava RN, editors. Bioremediation of Industrial Waste For Environmental Safety. Singapore: Springer (2020). p. 99–133.

PubMed Abstract | Google Scholar

22. European Food Safety Authority (EFSA). Opinion of the Scientific Panel on food additives, flavourings, processing aids and materials in contact with food (AFC) related to di-Butylphthalate (DBP) for use in food contact materials. EFSA J. (2005) 3:242. doi: 10.2903/j.efsa.2005.242

CrossRef Full Text | Google Scholar

23. European Food Safety Authority (EFSA). Opinion of the scientific panel on food additives, flavourings, processing aids and materials in contact with food (AFC) on a request from the commission related to butylbenzylphthalate (BBP) for use in food contact materials. EFSA J. (2005) 241:1–14. doi: 10.2903/j.efsa.2005.241

CrossRef Full Text | Google Scholar

24. European Food Safety Authority (EFSA). Opinion of the scientific panel on food additives, flavourings, processing aids and materials in contact with food (AFC) on a request from the commission related to di-isononylphthalate (DINP) for use in food contact materials. EFSA J. (2005) 244:1–18. doi: 10.2903/j.efsa.2005.244

CrossRef Full Text | Google Scholar

25. European Food Safety Authority (EFSA). Opinion of the scientific panel on food additives, flavourings, processing aids and materials in contact with food (AFC) on a request from the commission related to di-isodecylphthalate (DIDP) for use in food contact materials. EFSA J. (2005) 245:1–14. doi: 10.2903/j.efsa.2005.245

CrossRef Full Text | Google Scholar

26. European Food Safety Authority (EFSA). Opinion of the scientific panel on food additives, flavourings, processing aids and materials in contact with food (AFC) on a request from the commission related to bis(2-ethylhexyl)phthalate (DEHP) for use in food contact materials. EFSA J. (2005) 243:1–20. doi: 10.2903/j.efsa.2005.243

CrossRef Full Text | Google Scholar

27. Ibarra VG, de Quirós ARB, Losada PP, Sendón R. Identification of intentionally and non-intentionally added substances in plastic packaging materials and their migration into food products. Anal Bioanal Chem. (2018) 410:3789–803. doi: 10.1007/s00216-018-1058-y

PubMed Abstract | CrossRef Full Text | Google Scholar

28. Varshavsky JR, Morello-Frosch R, Woodruff TJ, Zota AR. Dietary sources of cumulative phthalates exposure among the US general population in NHANES 2005–2014. Environ Int. (2018) 115:417–29. doi: 10.1016/j.envint.2018.02.029

PubMed Abstract | CrossRef Full Text | Google Scholar

29. Alp AC, Yerlikaya P. Phthalate ester migration into food: effect of packaging material and time. Eur Food Res Technol. (2020) 246:425–35. doi: 10.1007/s00217-019-03412-y

CrossRef Full Text | Google Scholar

30. Perestrelo R, Silva CL, Algarra M, Câmara JS. Evaluation of the occurrence of phthalates in plastic materials used in food packaging. Appl Sci. (2021) 11:2130. doi: 10.3390/app11052130

CrossRef Full Text | Google Scholar

31. Carlos KS, de Jager LS, Begley TH. Determination of phthalate concentrations in paper-based fast food packaging available on the US market. Food Addit Contam A. (2021) 38:501–12. doi: 10.1080/19440049.2020.1859623

PubMed Abstract | CrossRef Full Text | Google Scholar

32. Gomez-Hens A, Aguilar-Caballos MP. Social and economic interest in the control of phthalic acid esters. Trend Anal Chem. (2003) 22:847–57. doi: 10.1016/S0165-9936(03)01201-9

CrossRef Full Text | Google Scholar

33. Tsai MY, Ho CH, Chang HY, Yang WC, Lin CF, Lin CT, et al. Analysis of Pollution of Phthalates in Pork and Chicken in Taiwan Using Liquid Chromatography–Tandem Mass Spectrometry and Assessment of Health Risk. Molecules. (2019) 24:3817. doi: 10.3390/molecules24213817

PubMed Abstract | CrossRef Full Text | Google Scholar

34. Guerreiro TM, de Oliveira DN, Melo CFOR, de Oliveira Lima E, Catharino RR. Migration from plastic packaging into meat. Food Res Int. (2018) 109:320–4. doi: 10.1016/j.foodres.2018.04.026

PubMed Abstract | CrossRef Full Text | Google Scholar

35. Giuliani A, Zuccarini M, Cichelli A, Khan H, Reale M. Critical review on the presence of phthalates in food and evidence of their biological impact. Inter J Env Res Pub Health. (2020) 17:5655. doi: 10.3390/ijerph17165655

PubMed Abstract | CrossRef Full Text | Google Scholar

36. Wypych A. Databook of Plasticizers. ChemTec Publishing:Toronto (2017). p. 710.

Google Scholar

37. Fierens T, Cornelis C, Standaert A, Sioen I, De Henauw S, Van Holderbeke M. Modelling the environmental transfer of phthalates and polychlorinated dibenzo-p-dioxins and dibenzofurans into agricultural products: the EN-forc model. Env Res. (2014) 133:282–93. doi: 10.1016/j.envres.2014.06.005

PubMed Abstract | CrossRef Full Text | Google Scholar

38. Moreira MA, André LC, de Lourdes Cardeal Z. Analysis of plasticiser migration to meat roasted in plastic bags by SPME–GC/MS. Food Chem. (2015) 178:195–200. doi: 10.1016/j.foodchem.2015.01.078

PubMed Abstract | CrossRef Full Text | Google Scholar

39. Edwards L, McCray NL, VanNoy BN, Yau A, Geller RJ, Adamkiewicz G, Zota AR. Phthalate and novel plasticizer concentrations in food items from U.S. fast food chains: a preliminary analysis. J Expo Sci Environ Epidemiol. (2021). doi: 10.1038/s41370-021-00392-8

PubMed Abstract | CrossRef Full Text | Google Scholar

40. Yang J, Song W, Wang X, Li Y, Sun J, Gong W, et al. Migration of phthalates from plastic packages to convenience foods and its cumulative health risk assessments. Food Addit Contam Part B Surveill. (2019) 12:151–8. doi: 10.1080/19393210.2019.1574909

PubMed Abstract | CrossRef Full Text | Google Scholar

41. Aurela B, Kulmala H, Soderhjelm L. Phthalates in paper and board packaging and their migration into Tenax and sugar. Food Addit Contam. (1999) 16:571–7. doi: 10.1080/026520399283713

PubMed Abstract | CrossRef Full Text | Google Scholar

42. Tiggelman I. Migration of Organic Contaminants Through Paper and Plastic Packaging [master's thesis]. [Stellenbosch], South Africa: University of Stellenbosch (2012).

Google Scholar

43. Gorlov IF, Fedotova GV, Slozhenkina MI, Mosolova NI. The meat products supply of population in Russia. In: Popkova EG, editor. Growth Poles of the Global Economy: Emergence, Changes and Future Perspectives. Cham: Springer (2020). p. 311–8.

Google Scholar

44. Walpole SC, Prieto-Merino D, Edwards P, Cleland J, Stevens G, Roberts I. The weight of nations: an estimation of adult human biomass. BMC Public Health. (2012) 12:439. doi: 10.1186/1471-2458-12-439

PubMed Abstract | CrossRef Full Text | Google Scholar

45. Antamoshkina EN, Rogachev AF. The model of statistical assessment of food security. In: Bogoviz AV, editor. Complex Systems: Innovation and Sustainability in the Digital Age. Cham: Springer (2020) p. 471–9.

Google Scholar

46. Grujić R, Vujadinović D, Savanović D. Biopolymers as food packaging materials. In: Pellicer E, Nikolic D, Sort J, Baró M, Zivic F, Grujovic N, Pelemis S, editors. Advances in Applications of Industrial Biomaterials. Cham: Springer (2017) p. 139–60.

Google Scholar

47. Vasiljevic L, Pavlovi S. Biodegradable Polymers Based on Proteins and Carbohydrates. In: Pellicer E, Nikolic D, Sort J, Baró M, Zivic F, Grujovic N, Pelemis S, editors. Advances in Applications of Industrial Biomaterials. Cham: Springer (2017) p. 87–101.

Google Scholar

48. Zabodalova L, Ishchenko T, Skvortcova N, Baranenko D, Chernjavskij V. Liposomal beta-carotene as a functional additive in dairy products. Agronomy Res. (2014) 12:825–34.

Google Scholar

49. Baranenko DA, Kolodyaznaya VS, Zabelina NA. Effect of composition and properties of chitosan-based edible coatings on microflora of meat and meat products. Acta Sci Pol Technol Alimen. (2013)12:149–57.

Google Scholar