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Front. Pharmacol. | doi: 10.3389/fphar.2018.01557

Seabuckthorn pulp oil protects against myocardial ischemia-reperfusion injury in rats through activation of Akt/eNOS

  • 1All India Institute of Medical Sciences, India
  • 2R. C. Patel Institute of Pharmaceutical Education and Research, India
  • 3College of Medicine & Health Sciences, UAE University, United Arab Emirates

Abstract
Seabuckthorn (SBT) pulp oil obtained from the fruits of seabuckthorn [Hippophae rhamnoides L. (Elaeagnaceae)] has been used traditionally for its medicinal and nutritional properties. However, its role in ischemia-reperfusion (IR) injury of myocardium in rats has not been elucidated so far.The present study reports the cardioprotective effect of SBT pulp oil in IR-induced model of myocardial infarction in rats and underlying mechanism mediating activation of Akt/eNOS signaling pathway. Male albino Wistar rats were orally administered SBT pulp oil (5, 10and 20 ml/kg/day) or saline for 30 days. On the day 31, ischemia was induced by one-stage ligation of left anterior descending coronary artery for 45 min followed by reperfusion for 60 min. SBT pulp oil pretreatment at the dose of 20 ml/kg observed to stabilize cardiac function and myocardial antioxidants such as glutathione, superoxide dismutase, catalase and inhibited lipid peroxidation evidenced by reduced malondialdehyde levels as compared to IR-control group. SBT pulp oil also improved hemodynamic and contractile function and decreased tumor necrosis factor and activities of myocyte injury marker enzymes; lactate dehydrogenase and creatine kinase-MB. Additionally, a remarkable rise in expression of pAkt-eNOS, Bcl-2 and decline in expression of IKKβ/NF-κB and Bax was observed in the myocardium. The histopathological and ultrastructural salvage of cardiomyocytes further supports the cardioprotective effect of SBT pulp oil. Based on findings, it can be concluded that SBT pulp oil protects against myocardial IR injury mediating favorable modulation of Akt-eNOS and IKKβ/NF-κB expression.

Keywords: Apoptosis, Natural Products, Myocardial ischemia–reperfusion injury, inflammatioin, Edible oil, Lehberry, Seabuckthorn, Oxidative Stress

Received: 20 Sep 2018; Accepted: 21 Dec 2018.

Edited by:

Martin C. Michel, Johannes Gutenberg University Mainz, Germany

Copyright: © 2018 suchal, Bhatia, Malik, Malhotra, Gamad, Goyal, Nag, Arya and Ojha. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Shreesh K. Ojha, College of Medicine & Health Sciences, UAE University, AlAin, United Arab Emirates, shreeshojha@uaeu.ac.ae