ORIGINAL RESEARCH article
Front. Nutr.
Sec. Food Chemistry
Volume 12 - 2025 | doi: 10.3389/fnut.2025.1548103
Ultrasound-assisted Enzymatic Extraction of Dark Tea Total Polyphenols
Provisionally accepted
- 1School of Chemistry and Bioengineering, Hunan University of Science and Engineering, Hunan, China
- 2Hunan University of Science and Engineering, Hunan, China
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This study used dark tea as the raw material and employed the Plackett Burman method to screen for 8 factors that may affect the extraction of dark tea total polyphenols. Box Behnken response surface methodology was used to further optimize the four most important variables. At a solid-liquid ratio of 1:50, enzyme dosage of 2.5%, pH of 5.6, enzymatic hydrolysis temperature of 45℃, enzymatic hydrolysis time of 50 min, ethanol volume fraction of 50%, ultrasonic temperature of 72℃, and ultrasonic time of 50 min, the highest extraction amount of dark tea total polyphenols was obtained.Compared with the ethanol reflux extraction method, the extraction amount of dark tea total polyphenols increased by about 43.38%, and compared with the ultrasonic assisted extraction method, the extraction amount of dark tea total polyphenols increased by about 30.45%. Ultrasonic assisted enzymatic extraction of dark tea total polyphenols has the strongest antioxidant activity.
Keywords: Dark tea, Ultrasound-assisted enzymatic method, Polyphenols, antioxidant activity, Response Surface Methodology
Received: 19 Dec 2024; Accepted: 30 May 2025.
Copyright: © 2025 Shao, Xiao, Li, Chen, Xing, Zhu and Qi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Zhiyong Zhu, Hunan University of Science and Engineering, Hunan, China
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