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ORIGINAL RESEARCH article

Front. Nutr.

Sec. Sport and Exercise Nutrition

Volume 12 - 2025 | doi: 10.3389/fnut.2025.1588421

Six Weeks of Either EPA-rich or DHA-rich Omega-3 Supplementation Alters Submaximal Exercise Physiology in Endurance Trained Male Amateurs

Provisionally accepted
  • 1University of Birmingham, Birmingham, United Kingdom
  • 2Swiss Distance University of Applied Sciences, Brig, Switzerland

The final, formatted version of the article will be published soon.

Purpose: Supplementation with omega-3 Omega-3 fatty acids such as Docosahexaenoic acid (DHA) and/or Eicosapentaenoic acid (EPA) have been shown to lower submaximal exercise heart rate (HR) and whole-body oxygen consumption along with other positive exercise physiology adaptations. However, the impact of supplementation on exercise physiology is inconsistent. This could be due to existing study heterogeneity, including inconsistent use of EPA or DHA supplements. The current study aimed to investigate if EPA-rich or DHA-rich supplements are equally efficient in at eliciting modifying physiological responses to submaximal exercise and potentially improving performance.Methods: Fifty-five endurance trained amateurs participated in a submaximal exercise test followed by a 24 km cycling time trial (TT) before and after a six-week supplementation period. Participants were supplemented with either 3g/day EPA-rich fish oil, DHA-rich algae oil, or a coconut oil placebo. Omega-3 index, submaximal exercising HR, rating of perceived exertion (RPE), respiratory exchange ratio (RER), and time trialTT performance were all assessed during exercise.Results: The EPA-rich and DHA-rich supplements significantly increased the omega-3 Omega-3 index, EPA and DHA content of red blood cells (RBCs), whereas the placebo supplement had no effect. Statistically significant changes between pre-and postsupplementation were found in submaximal exercise physiology. Both EPA-rich and DHArich supplementation lowered submaximal exercising HR (∆=-4, P=.005) (∆=-9, P≤.001) and RPE (∆=-0.7, P≤.001) (∆=-0.9, P≤.001), while only EPA-rich supplementation increased RER (∆=+0.03, P≤.001). Change in Omega-3 index inversely correlated with both change in submaximal exercising HR (RHO=-0.43, P=0.007) and RPE (RHO=-0.40, P=0.013). Time trialTT performance improved in all three conditions, but there were no significant differences in the gains across the three conditions.1 Conclusion: This study adds further evidence that both EPA and DHA can alter submaximal exercise physiology, but further research is required to determine their effects on exercise performance outcomes.

Keywords: omega-3 index, EPA, DHA, Submaximal exercise, Heart Rate, performance

Received: 05 Mar 2025; Accepted: 01 Aug 2025.

Copyright: © 2025 Boulton, Thielecke and Blannin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Andy Blannin, University of Birmingham, Birmingham, United Kingdom

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