ORIGINAL RESEARCH article
Front. Nutr.
Sec. Clinical Nutrition
Volume 12 - 2025 | doi: 10.3389/fnut.2025.1592045
Establishment of Age-Stratified Serum Vitamin B6 Pyridoxal and Pyridoxic Acid Reference Intervals among Children Aged 1-16 Years in Henan, China
Provisionally accepted
- 1Children’s Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan Province, China
- 2Children’s Hospital Affiliated of Zhengzhou University, Zhengzhou, China
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Background/Objective: The development of population-specific reference intervals (RIs) for serum vitamin B6 (B6) in Chinese pediatric populations has not been established. Current clinical practice relies on international thresholds, but diagnostic sensitivity with domestic biochemical profiles remains suboptimal.This study aims to establish age-stratified reference intervals for serum vitamin B6 pyridoxal (B6-PL) and pyridoxic acid (B6-PA) in Henan children aged 1-16 years.: This retrospective study analyzed 1,458 healthy children undergoing routine physical examinations at Henan Children's Hospital from December 2021 to June 2024. Serum B6-PL and B6-PA were quantified using the Xevo TQ-S Micro liquid chromatography-tandem mass spectrometry system (Waters Corporation). Non-normally distributed data are expressed as median (interquartile range) [M (P25, P75)]. Age-and sexrelated differences were assessed using Mann-Whitney U or Kruskal-Wallis tests.Results: Analysis revealed that age significantly influences serum levels of B6-PL and B6-PA in children, while gender showed no significant effect. The pediatric cohort demonstrated a male predominance (58.98%, 860/1458) with a notable prepubertal skew (93.28%, 1360/1458 aged <12 years). Further analysis facilitated the establishment of reference intervals (RIs) for B6-PL and B6-PA across different age groups. The RIs for B6-PL were 1.62-17.70 ng/mL for children aged 1-5 years, 1.39-15.15 ng/mL for children aged 6-11 years, and 1.06-14.30 ng/mL for children aged 12-16 years. The RIs for B6-PA were 1.20-5.46 ng/mL for children aged 1-5 years, 1.10-4.41 ng/mL for children aged 6-11 years, and 0.95-4.99 ng/mL for children aged 12-16 years.This study establishes the first age-stratified reference intervals (RIs) for serum vitamin B6 metabolites (B6-PL and B6-PA) among children aged 1-16 years in Henan, China.
Keywords: Vitamin B6 pyridoxal, Vitamin B6 pyridoxic Acid, Reference intervals, Children, Henan
Received: 13 Mar 2025; Accepted: 14 Jul 2025.
Copyright: © 2025 Luan, Chen, Wang, Li, Yang and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Junmei Yang, Children’s Hospital Affiliated of Zhengzhou University, Zhengzhou, China
Tiewei Li, Children’s Hospital Affiliated of Zhengzhou University, Zhengzhou, China
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