CASE REPORT article

Front. Nutr., 19 May 2025

Sec. Nutrition and Microbes

Volume 12 - 2025 | https://doi.org/10.3389/fnut.2025.1594022

This article is part of the Research TopicDietary Modulation of Gut Microbiota-X axisView all 12 articles

Therapeutic efficacy of fecal microbiota transplantation in severe food intolerance: a case report


Yanhui Huang,&#x;Yanhui Huang1,2†Jiayuan Huang&#x;Jiayuan Huang1†Yuange LiYuange Li1Tianyu XuTianyu Xu1Guoqiao QuanGuoqiao Quan1Peihao XuPeihao Xu1Xiaoya Yang*Xiaoya Yang3*Zhou Liu*Zhou Liu4*Wenrui Xie*Wenrui Xie1*
  • 1Department of Gastroenterology, Research Center for Engineering Techniques of Microbiota-Targeted Therapies of Guangdong, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, China
  • 2School of Clinical Medicine, Guangdong Medical University, Zhanjiang, China
  • 3Department of Physiology, Guangzhou Health Science College, Guangzhou, China
  • 4Department of Neurology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, China

This report presents the first documented application of fecal microbiota transplantation (FMT) for the management of extensive multi-food intolerance involving 52 specific foods in a pediatric patient with autism spectrum disorder (ASD). A 7 years-old autistic child was diagnosed with food intolerance to 52 items, presenting with generalized rashes, diarrhea, and malnutrition (BMI of 12.9) upon exposure or ingestion of the implicated foods. The child received oral fecal microbiota capsule treatment, with a daily dose of nine capsules (a total of 120 capsules per course) for two consecutive treatment courses. The rashes resolved, the child regained tolerance to previously intolerable foods, nutritional status improved, and stool consistency normalized. This case suggests that FMT may hold therapeutic potential for managing food intolerance in autistic patients.

1 Introduction

Food intolerance (FI) refers to non-immune-mediated adverse reactions to food or food components at normal tolerated doses, including metabolic, toxic, pharmacological, and undefined mechanisms (16). It’s prevalence ranges from 15% to 45% (7). The main mechanisms underlying FI include these following aspects: Firstly, Certain foods contain pharmacologically active com-pounds (e.g., histamine, monosodium glutamate, caffeine) that can induce physiological responses like smooth muscle contraction and inflammation, contributing to FI (8). Secondly, Lactase deficiency is a major cause of lactose. Similarly, deficiencies in histamine-degrading enzymes, such as diamine oxidase (DAO) and histamine-N-methyltransferase (HNMT), can lead to histamine accumulation and FI symptoms (9). What’s more, Food additives and preservatives (e.g., nitrates, nitrites) may also induce FI reactions (10, 11). The precise mechanisms remain unclear, but these components can trigger adverse reactions in susceptible individuals. Diagnosis of FI requires detailed medical history, dietary and lifestyle assessments, and laboratory tests (e.g., blood and stool analyses) or imaging to exclude organic diseases and food allergies (FA) (12). Currently, the double-blind, placebo-controlled oral challenge (DBPCFC) is considered the gold standard for FI diagnosis, involving food elimination and gradual reintroduction to identify triggers (13). Food-specific IgG or IgG4 serological testing is widely used in clinical practice (14).

The gut microbiota plays a crucial role in the pathogenesis of FI. FI is often associated with gut dysbiosis, characterized by the reduction or increase of specific microbial populations, leading to impaired gut barrier function, enhanced inflammatory responses (5, 15), and abnormal metabolic functions. FMT, as a therapeutic approach to modulate the gut microbiota, may restore microbial balance and improve FI symptoms by transplanting healthy donor microbiota into the recipient. Annabel Clancy and Thomas Borodyhave (16) demonstrated that FMT can significantly alleviate FI symptoms in patients with irritable bowel syndrome (IBS), highlighting the potential therapeutic value of gut microbiota in FI. FMT can also improve both autism symptoms and gastrointestinal symptoms in patients with ASD (17).

To date, there have been no reports on improvements in food intolerances in patients with ASD treated with FMT. Therefore, this case report of improvement in food intolerance and ASD symptoms after FMT in a male patient constitutes an important insight into a possible involvement of the gut microbiome in the pathogenesis of food intolerances.

2 Case report

The patient is a male born in 2017. Family history was negative for allergies or psychiatric disorders.

This study was approved by the Ethics Committee of the First Affiliated Hospital of Guangdong Pharmaceutical University [Approval No. 2923JS (11)]. And the informed consent form for FMT was signed by the patient’s legal guardian (PDF1).

In October 2019, he was diagnosed with “Autistic Spectrum Disorder” due to symptoms including poor language communication skills, irritability, social withdrawal, loose stools, multiple scattered eczema lesions and sleep disturbances. In December 2019, a 90-item food-specific IgG antibody test identified intolerance to 52 foods (Table 1), with severe reactivity to staples like rice, wheat, and milk. Elimination of these foods improved language expression, eczema, and sleep quality. Reintroduction of intolerant foods consistently provoked rashes and sleep disturbances, confirming provocation test results. Additionally, allergen-specific IgE antibody testing for inhaled and ingested allergens showed no significant abnormalities. The delayed diagnosis of FI in this pediatric patient with ASD and communication impairments was attributable to its less overt clinical manifestations compared to food allergy, compounded by age-related diagnostic challenges. The patient adheres to a structured food diary with rotation of tolerated foods. The patient presents with persistent generalized rashes, frequent diarrheal episodes, and an immunocompromised state, leading to multiple hospitalizations for recurrent pneumonia.

TABLE 1
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Table 1. Positive items of 90-item food-specific IgG antibody test.

In July 2024, the child received oral fecal microbiota capsule treatment.

Dosage and administration: three capsules per dose, three times daily, administered with warm water 30 min prior to meals. Each treatment course consists of 120 capsules (4.2 × 1013 CFU/course) (PDF2–Daily Microbial Suspension Logbook), with the therapy to be continued consecutively for two complete courses. Capsule specification: No. 3 pediatric-sized capsules are to be utilized for encapsulation.

The donor microbiota was sourced from a rigorously screened healthy adult who had no comorbidities or disorders known to be associated with changes in gut microbiota, were chosen as donors. Donor stools were screened for enteric pathogens including parasites (Entamoeba histolytica, Giardia) and bacteria (Salmonella, Shigella, Escherichia coli, Campylobacter, Yersinia, and C. difficile). The donors were accepted only if HAV IgM, HBsAg, anti-HCV antibodies, anti-human immunodeficiency virus antibodies, IgM antibodies against cytomegalovirus and tests for syphilis were negative. The stool sample was not accepted if donors had taken antibiotics or probiotics in previous 3 months. FMT possesses repeated microfiltration, centrifugation, washing, discarding resuspension and capsules preparation based on the automatic microfiltration machine (GenFMTer, Nanjing, China) in a biosafety level-3 laboratory (18, 19) and prepared by the Microecology Center of the First Affiliated Hospital of Guangdong Pharmaceutical University. Viable bacterial counts in all capsule preparations were validated to meet international standards (18, 20) prior to lyophilization. Furthermore, proactive donor fecal sample screening was implemented in response to real-time adverse event monitoring, with no FMT-related adverse events reported to date.

September 2024: After two courses of FMT capsules, the child’s symptoms significantly improved. The child tolerated previously intolerant foods (e.g., rice, wheat, soy, peanuts, and milk). No new rashes appeared, the existing rashes resolved (Figure 1), stool consistency normalized (Figure 1), and nutritional status improved with a gradual in-crease in body mass index (BMI) (Table 2).

FIGURE 1
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Figure 1. Changes in maculopapular rash and fecal characteristics after taking fecal microbiota capsules. (A–D) Maculopapular rash on the trapezius region: (A) on 2024-07-22; (B) on 2024-08-07; (C) on 2024-08-22; (D) on 2025-03-22. (E–H) Maculopapular rash on both lower legs: (E) on 2024-07-22; (F) on 2024-08-22; (G) on 2024-09-22; (H) on 2025-03-22. (I–L) Fecal characteristics: (I) on 2024-07-21; (J) on 2024-08-22; (K) on 2024-10-21; (L) on 2025-3-22.

TABLE 2
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Table 2. Changes in body mass index (BMI) after taking in the fecal microbiota capsules.

During the therapeutic course, the patient exhibited good tolerability and maintained high adherence to the prescribed pharmacological regimen. The legal guardian reported significant improvement in the patient’s rash condition post-capsule administration, with no pruritus or other adverse symptoms, demonstrating good acceptance. No adverse reactions were observed during the treatment.

December 2024: A repeat 90-item food-specific IgG antibody test showed significant improvement after two courses of fecal microbiota transplantation (Table 1). Post-FMT reassessment of food-specific IgG antibodies demonstrated a marked reduction in both the number and magnitude of food intolerances (Table 3).

TABLE 3
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Table 3. The alterations in the 90-item food-specific IgG antibody panel were analyzed before and after the administration of fecal microbiota capsules.

The chronological summary of the patient’s previous diagnostic and therapeutic interventions is systematically outlined in Figure 2.

FIGURE 2
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Figure 2. Timeline of clinical findings and nutritional intervention.

3 Discussion

This case report demonstrates notable clinical and methodological advancements in managing severe FI through FMT. Notably, it represents the first documented application of FMT for severe FI (52 items) in pediatric ASD, addressing a critical therapeutic gap in this complex patient population. Furthermore, the non-invasive oral capsule administration protocol overcomes procedural limitations typically encountered in ASD patients, enhancing clinical feasibility while maintaining therapeutic efficacy. Importantly, the intervention achieved multidimensional improvements encompassing cutaneous (rash resolution), gastrointestinal (stool normalization), immunological (restored food tolerance), and nutritional (BMI elevation) domains, suggesting systemic biological effects beyond symptomatic relief. Additionally, the standardized dosing regimen (nine capsules/day × 120 capsules/course × 2 courses) establishes a replicable framework for future trials. This correlation between microbiota modulation and regained oral tolerance aligns mechanistically with emerging evidence on gut microbiome-mediated antigen processing, thereby strengthening the biological plausibility of FMT as a disease-modifying therapy for FI.

Food intolerance can be caused by a variety of mechanisms, including enzyme deficiencies (e.g., lactase deficiency), pharmacological effects, irritant reactions, and toxicological responses. The gut microbiota plays a pivotal role in the pathogenesis of food intolerance.

The gut microbiota is involved in the pathogenesis of food intolerance caused by lactase deficiency. Lactase deficiency leads to undigested lactose interacting with the intestinal microbiota then produce short-chain fatty acids (SCFA) (acetate, propionate) and gases (hydrogen, carbon dioxide). When the amount of lactose exceeds the fermentation capacity of the colonic microbiota, or when the absorption capacity for SCFA in the colon is overwhelmed, diarrhea occurs. Brandao Gois et al. (21) found that, higher Bifidobacterium abundance in lactose intolerance individuals (P Wilcox = 4.56 × 10–9).

The gut microbiota participates in the pathogenesis of FI associated with Non-Celiac Gluten Sensitivity (NCGS): M. Daulatzai et al. (22) found that NCGS patients exhibit gut microbiota dysbiosis, characterized by reduced beneficial bacteria (e.g., Bifidobacterium) and increased pro-inflammatory bacteria (e.g., Enterobacteriaceae, Escherichia coli, and Firmicutes). This dysbiosis may contribute to bloating through enhanced fermentation. Molecular studies highlight increased Claudin-4, a tight junction protein regulating paracellular permeability, supporting the “leaky gut” hypothesis. Hansen et al. (23) demonstrated that an 8 weeks low-gluten diet in 60 Danish adults reduced fecal Bifidobacterium, Dorea, Blautia, Lachnospiraceae, and butyrate-producing bacteria (Anaerostipes hadrus, Eubacterium hallii), alongside decreased postprandial hydrogen breath and bloating.

The gut microbiota is involved in the pathogenesis of FI induced by FODMAPs. FODMAPs, short-chain carbohydrates including lactose, fructose, sugar alcohols, fructans, and galacto-oligosaccharides (GOS), are naturally present in fruits, vegetables, grains, and dairy products. Their malabsorption results from deficiencies in brush border enzymes (e.g., lactase, sucrase) in the small intestine, leading to osmotic water retention and diarrhea. Dysregulated gut microbiota further exacerbates this condition, as pathogenic bacteria ferment undigested FODMAPs in the colon, producing excessive hydrogen, methane, and acidic by-products. This accumulation of gas, liquid, and acids stimulates the intestinal wall, causing FI symptoms such as bloating, abdominal pain, and diarrhea. The gut microbiota is implicated in the pathogenesis of histamine intolerance.

Histamine intolerance arises from impaired histamine degradation due to reduced activity or levels of histamine-metabolizing enzymes. Consumption of histamine-rich foods, or DAO-inhibiting medications elevates exogenous histamine. In these patients, gut dysbiosis is characterized by increased Proteus bacteria, damaging epithelial cells and DAO production. Such dysregulation exacerbates endogenous histamine levels, worsening FI symptom (24). Histamine-intolerant individuals also exhibit reduced beneficial bacteria (e.g., Prevotellaceae, Ruminococcus, Faecalibacterium prausnitzii) and increased histamine-secreting bacteria (e.g., Staphylococcus, Proteus, Clostridium perfringens, Enterococcus faecium) (25).

The association between gut microbiota dysbiosis and FI is increasingly recognized, highlighting the potential of microbiota-targeted therapies in managing FI. Probiotics and prebiotics have been explored as treatments since 2001 (26), with recent studies supporting probiotic supplementation for lactose intolerance (27). Animal studies further validate their efficacy. Ardizzone et al. (28) demonstrated that a novel therapeutic formulation (NTN) containing Lactobacillus acidophilus and Lactobacillus reuteri restored intestinal barrier integrity and permeability in mice with diet-induced FI, alleviating related symptoms. Ferrari et al. (29) highlighted probiotics’ role in modulating gut microbiota, reducing ER stress, mitigating inflammation, and enhancing barrier function, collectively improving FI. Additionally, Besseling-van der Vaart et al. (30) showed that the multi-strain probiotic Ecologic® Tolerance (Syngut™) enhanced β-galactosidase activity, strengthened epithelial barriers, and improved resistance to digestive enzymes and bile salts in vitro.

These findings collectively underscore the therapeutic potential of probiotics in addressing gut microbiota dysbiosis and alleviating FI symptoms, providing a foundation for further clinical investigations.

After two consecutive treatment of oral fecal microbiota capsule, retesting of 90-food-specific IgG antibodies revealed decreased intolerance levels to most dietary antigens. Paradoxically, cashews and almonds progressed from moderate to severe intolerance, while corn, mushrooms, and capsaicin transitioned from tolerable to mild intolerance. We hereby propose the following discussion points.

Cashew and almond intolerance progressed from moderate to severe. These nuts are rich in lectins, which are resistant to high temperatures and enzymatic digestion in both rodents and humans (31). Emerging evidence highlights that children with ASD frequently exhibit depletion of butyrate-producing commensals, particularly Faecalibacterium prausnitzii (32). Post-FMT fluctuations in SCFA concentrations, notably butyrate, may activate GPR41/GPR43 receptors to modulate host energy metabolism and anti-inflammatory responses (33). However, altered intestinal transit time secondary to SCFA shifts could paradoxically prolong luminal exposure to undegraded dietary lectins. Lectins can translocate across the intestinal barrier into the bloodstream, where they deposit on blood and lymphatic vessel walls, stimulating the immune system (34), ultimately leading to elevated IgG levels. Lectins, through binding to glycans on the intestinal mucosa, may disrupt mucin polysaccharide architecture, impairing bacterial adhesion and proliferation (35), while concurrently inhibiting brush-border enzyme activity (e.g., disaccharidases), thereby exacerbating maldigestion and nutrient malabsorption (36, 37). Furthermore, lectin-mediated agglutination of beneficial symbionts may reduce their ecological fitness (38, 39), creating niches for opportunistic pathogens such as Escherichia coli and Lactobacillus spp. to proliferate. This dysbiosis disrupts microbial equilibrium, thereby contributing to fluctuations in IgG levels (40).

What’s more, New-onset mild intolerance to mushroom, corn, and capsaicin was observed. Notably, chitin—a fungal polysaccharide abundant in mushrooms—alters microbial community structure (41), and incomplete engraftment of donor-derived chitinolytic taxa could manifest as transient reactivity to mushroom components. The metabolism of resistant starch (e.g., maize-derived) relies on Clostridium butyricum-encoded amylases (42), whereas capsaicin a TRPV1 receptor agonist, demonstrates tolerability closely linked to gut microbiota composition.(43). FMT-induced dysbiosis may disrupt these specialized metabolic pathways, potentially explaining transient post-FMT intolerances.

Current understanding of post-FMT microbiota engraftment remains incomplete. Analogous to organ transplantation, FMT faces inherent challenges of “microbiota rejection,” wherein host immune and ecological factors limit donor strain persistence (44). Chen et al. demonstrated that donor strain engraftment rates rarely exceed 65%, with most clinical cohorts achieving < 30% colonization (45). While microbial network topology may attain dynamic equilibrium within weeks to 3 months post-FMT, functional stabilization (e.g., metabolic cross-feeding networks) likely requires extended timelines (46), paralleling gradual host physiological recovery. Delayed colonization of keystone taxa (e.g., F. prausnitzii) may thus underpin de novo food reactivity during this transitional phase.

Above all, these findings indicate that dysbiosis of the gut microbiota plays a significant role in the development of various types of FI. The mechanisms of FI in this case suggest a multifactorial origin of FI. FMT may be an effective intervention to restore microbial balance, reduce fermentation, repair the intestinal barrier, and reduce the levels of pro-inflammatory markers such as IFN-γ and histamine. This treatment likely contributed to the significant improvement in the child’s symptoms, including the resolution of rashes, normalization of stool consistency, and improvement in BMI. The successful clinical response supports the role of microbiota modulation in the treatment of complex FIs.

Fecal microbiota transplantation is a promising therapy for chronic diseases associated with gut microbiota alterations (47). Comparative analysis of FMT across distinct disease entities is necessary. Key mechanistic insights are summarized below: The therapeutic mechanism of FMT in IBS emphasizes gut dysbiosis-driven visceral hypersensitivity, characterized by overproliferation of Gram-negative bacteria such as Proteus mirabilis and depletion of probiotics including Lactobacillus rhamnosus GG. Dysregulated microbial metabolites, such as LPS, suppress resolvin D1 synthesis in colonic tuft cells via TLR4/MyD88 signaling, perpetuating inflammatory cascades and nociception (48).

In the management of FI,FMT primarily enhances intestinal barrier function and energy metabolism through probiotic engraftment to cure FI. For instance, lactose intolerance improvement correlates with increased Bifidobacterium abundance, whose β-galactosidase activity facilitates lactose digestion without generating gas byproducts (e.g., hydrogen, carbon dioxide, methane) that drive bloating.

In the management of ASD,FMT reshapes gut microbiota by enriching beneficial taxa (Bacteroides fragilis, Lactobacillus reuteri) while suppressing pathobionts (Clostridiales, Eubacterium coprostanoligenes). This modulates neuroactive metabolites (4-EPS, SCFAs) and neurotransmitters (serotonin, dopamine), restoring gut barrier integrity and suppressing inflammation. These effects synergistically ameliorate ASD core symptoms and gastrointestinal comorbidities via vagal nerve and hypothalamic-pituitary-adrenal (HPA) axis cross-talk.

Emerging evidence highlights that increased abundance of Bifidobacterium (4951) in the gut microbiota is associated with clinical improvement in FI, IBS, and ASD. Notably, reduced Faecalibacterium (5254) levels have been consistently reported across these three conditions, while ASD-specific dysbiosis is further characterized by overproliferation of Sutterella (55). Current research confirms the therapeutic efficacy of Bacteroides fragilis strain BF839 in ASD intervention; however, no specific microbial strains have yet been identified for targeted management of IBS or FI.

However, this study has several limitations. First, this study’s sample size was small, and studies with larger sizes and control group are needed for further exploration. Second, 8 months’ observations can’t fully reflect the effects of FMT on devel-oping FI symptoms. Studies with longer follow-up are needed to characterize the long-term efficacy and safety of FMT for pediatric patients. Finally, This case lacked pretreatment assessment of DAO, zonulin, endotoxin, serum histamine, SCFAs, and gut microbiota composition, The absence of zonulin and LPS measurements in this study may limit comprehensive evaluation of intestinal barrier integrity and systemic inflammation. For instance, Fasano et al. (56) demonstrated that zonulin serves as a sensitive biomarker of intestinal permeability, with dynamic changes reflecting FMT-induced mucosal repair. LPS, a key driver of gut hyperpermeability, inhibits tight junction proteins (e.g., occludin, claudin-1) via the TLR4/MyD88 pathway, directly compromising barrier function (45). The lack of LPS quantification precludes definitive conclusions regarding FMT-mediated LPS reduction and tight junction restoration.

Similarly, SCFA levels—critical mediators of microbiota-driven immune and barrier regulation (57) were not assayed. While clinical improvements (e.g., reduced diarrhea, enhanced behavioral scores) may correlate with SCFA restoration (e.g., butyrate, propionate), the absence of direct SCFA data impedes mechanistic validation. Histamine levels were also unmeasured. Histamine intolerance mechanisms include exogenous intake (high-histamine foods), dysbiosis, intestinal hyperpermeability, gastrointestinal bleeding, or mastocytosis. This omission constrains deeper mechanistic exploration (13).

Diamine oxidase levels hold significant reference value in diagnosing HIT, yet their clinical utility necessitates comprehensive multidisciplinary evaluation. Studies demonstrate that HIT patients exhibit markedly lower serum DAO levels compared to healthy controls, and strict dietary intervention correlates with DAO elevation alongside symptom remission, suggesting DAO as a biomarker for monitoring dietary compliance and therapeutic efficacy (58, 59). However, DAO’s diagnostic sensitivity remains constrained: only 50%–71% of HIT patients present DAO < 10 U/mL, while subnormal DAO levels are also observed in asymptomatic populations, resulting in a low positive predictive value for standalone testing (59, 60). Thus, DAO testing should serve as a complementary tool, integrated with clinical symptomatology, dietary provocation trials, and exclusion diagnostics to enhance diagnostic precision (58, 59, 61). These limitations mirror broader technical challenges in FMT research and underscore the necessity of standardized multi-omics platforms for mechanistic elucidation.

Due to the resource constraints and the patient’s outpatient clinical follow-up protocol, which precluded comprehensive analysis of fecal gut microbiota composition. The absence of microbiota profiling hinders mechanistic exploration of microbial metabolites in symptom amelioration. However, the single-case design inherently limits the statistical power required for microbiota-symptom correlation analyses. Future multicenter trials with serial metagenomic and metabolomic profiling will elucidate microbial drivers of therapeutic responses.

Data availability statement

The original contributions presented in this study are included in this article/Supplementary material, further inquiries can be directed to the corresponding authors.

Ethics statement

The studies involving humans were approved by. The studies involving human participants were reviewed and approved by the Ethics Committee of the First Affiliated Hospital of Guangdong Pharmaceutical University [reference number: 2923JS(11)]. The participant provided written informed consent to participate in this study. The studies were conducted in accordance with the local legislation and institutional requirements. Written informed consent for participation in this study was provided by the participants’ legal guardians/next of kin. Written informed consent was obtained from the individual(s), and minor(s)’ legal guardian/next of kin, for the publication of any potentially identifiable images or data included in this article.

Author contributions

YH: Conceptualization, Formal Analysis, Resources, Writing – original draft, Writing – review and editing. JH: Formal Analysis, Methodology, Writing – original draft, Writing – review and editing. YL: Data curation, Writing – original draft, Writing – review and editing. TX: Data curation, Writing – original draft. GQ: Data curation Writing – original draft. PX: Data curation, Writing – original draft. XY: Writing – review and editing. ZL : Writing – review and editing. WX: Writing – review and editing.

Funding

The author(s) declare that financial support was received for the research and/or publication of this article. This work was supported by Tertiary Education Scientific Research Project of Guangzhou Municipal Education Bureau (Nos. 202235423 and 202032875).

Acknowledgments

We would like to thank all those who participated in the studies, in particular our study subjects.

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Generative AI statement

The authors declare that no Generative AI was used in the creation of this manuscript.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Supplementary material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fnut.2025.1594022/full#supplementary-material

References

1. Boyce J, Assa’a A, Burks A, Jones S, Sampson H, Wood R, et al. Guidelines for the diagnosis and management of food allergy in the United States: Summary of the NIAID-Sponsored Expert Panel Report. Nutrition. (2011) 27:253–67. doi: 10.1016/j.nut.2010.12.001

PubMed Abstract | Crossref Full Text | Google Scholar

2. Lacy B. The science, evidence, and practice of dietary interventions in irritable bowel syndrome. Clin Gastroenterol Hepatol. (2015) 13:1899–906. doi: 10.1016/j.cgh.2015.02.043

PubMed Abstract | Crossref Full Text | Google Scholar

3. Turnbull J, Adams H, Gorard D. Review article: The diagnosis and management of food allergy and food intolerances. Aliment Pharmacol Ther. (2015) 41:3–25. doi: 10.1111/apt.12984

PubMed Abstract | Crossref Full Text | Google Scholar

4. Wong K, Horwitz R, Soffer G. Immunoglobulin G food testing. Ann Allergy Asthma Immunol. (2021) 126:611–2. doi: 10.1016/j.anai.2021.01.022

PubMed Abstract | Crossref Full Text | Google Scholar

5. Tuck C, Biesiekierski J, Schmid-Grendelmeier P, Pohl D. Food intolerances. Nutrients. (2019) 11:1684. doi: 10.3390/nu11071684

PubMed Abstract | Crossref Full Text | Google Scholar

6. Hon E, Gupta S. Gastrointestinal food allergies and intolerances. Gastroenterol Clin North Am. (2021) 50:41–57. doi: 10.1016/j.gtc.2020.10.006

PubMed Abstract | Crossref Full Text | Google Scholar

7. Jansson-Knodell C, White M, Lockett C, Xu H, Shin A. High prevalence of food intolerances among US internet users. Public Health Nutr. (2021) 24:531–5. doi: 10.1017/S1368980020003298

PubMed Abstract | Crossref Full Text | Google Scholar

8. Sánchez-Pérez S, Celorio-Sardà R, Veciana-Nogués M, Latorre-Moratalla M, Comas-Basté O, Vidal-Carou M. 1-methylhistamine as a potential biomarker of food histamine intolerance. A pilot study. Front Nutr. (2022) 9:973682. doi: 10.3389/fnut.2022.973682

PubMed Abstract | Crossref Full Text | Google Scholar

9. Maintz L, Novak N. Histamine and histamine intolerance. Am J Clin Nutr. (2007) 85:1185–96. doi: 10.1093/ajcn/85.5.1185

PubMed Abstract | Crossref Full Text | Google Scholar

10. Lerner A, Matthias T. Changes in intestinal tight junction permeability associated with industrial food additives explain the rising incidence of autoimmune disease. Autoimmun Rev. (2015) 14:479–89. doi: 10.1016/j.autrev.2015.01.009

PubMed Abstract | Crossref Full Text | Google Scholar

11. Chassaing B, Koren O, Goodrich J, Poole A, Srinivasan S, Ley R, et al. Dietary emulsifiers impact the mouse gut microbiota promoting colitis and metabolic syndrome. Nature. (2015) 519:92–6. doi: 10.1038/nature14232

PubMed Abstract | Crossref Full Text | Google Scholar

12. Lomer M. Review article: The aetiology, diagnosis, mechanisms and clinical evidence for food intolerance. Aliment Pharmacol Ther. (2015) 41:262–75. doi: 10.1111/apt.13041

PubMed Abstract | Crossref Full Text | Google Scholar

13. Gargano D, Appanna R, Santonicola A, De Bartolomeis F, Stellato C, Cianferoni A, et al. Food allergy and intolerance: A narrative review on nutritional concerns. Nutrients. (2021) 13:1638. doi: 10.3390/nu13051638

PubMed Abstract | Crossref Full Text | Google Scholar

14. Qin L, Tang L, Cheng L, Wang H. The clinical significance of allergen-specific IgG4 in allergic diseases. Front Immunol. (2022) 13:1032909. doi: 10.3389/fimmu.2022.1032909

PubMed Abstract | Crossref Full Text | Google Scholar

15. Ohtsuka Y. Food intolerance and mucosal inflammation. Pediatrics Int. (2015) 57:22–9. doi: 10.1111/ped.12546

PubMed Abstract | Crossref Full Text | Google Scholar

16. Clancy A, Borody T. Improvement in food intolerance symptoms after pretreatment with antibiotics followed by faecal microbiota transplantation: A case report. Case Rep Clin Nutr. (2021) 4:7–13. doi: 10.1159/000517306

Crossref Full Text | Google Scholar

17. Kang D, Adams J, Gregory A, Borody T, Chittick L, Fasano A, et al. Microbiota transfer therapy alters gut ecosystem and improves gastrointestinal and autism symptoms: An open-label study. Microbiome. (2017) 5:10. doi: 10.1186/s40168-016-0225-7

PubMed Abstract | Crossref Full Text | Google Scholar

18. Shi Q. Nanjing consensus on methodology of washed microbiota transplantation. Chinese Med J. (2020) 133:2330–2. doi: 10.1097/CM9.0000000000000954

PubMed Abstract | Crossref Full Text | Google Scholar

19. Zhang F, Cui B, He X, Nie Y, Wu K, Fan D. Microbiota transplantation: Concept, methodology and strategy for its modernization. Protein Cell. (2018) 9:462–73. doi: 10.1007/s13238-018-0541-8

PubMed Abstract | Crossref Full Text | Google Scholar

20. Cammarota G, Ianiro G, Kelly C, Mullish B, Allegretti J, Kassam Z, et al. International consensus conference on stool banking for faecal microbiota transplantation in clinical practice. Gut. (2019) 68:2111–21. doi: 10.1136/gutjnl-2019-319548

PubMed Abstract | Crossref Full Text | Google Scholar

21. Brandao Gois M, Sinha T, Spreckels J, Vich Vila A, Bolte L, Weersma R, et al. Role of the gut microbiome in mediating lactose intolerance symptoms. Gut. (2022) 71:215–7. doi: 10.1136/gutjnl-2020-323911

PubMed Abstract | Crossref Full Text | Google Scholar

22. Daulatzai M. Non-Celiac gluten sensitivity triggers gut dysbiosis, neuroinflammation, gut-brain axis dysfunction, and vulnerability for dementia. Cnsnddt. (2015) 14:110–31. doi: 10.2174/1871527314666150202152436

PubMed Abstract | Crossref Full Text | Google Scholar

23. Hansen L, Roager H, Søndertoft N, Gøbel R, Kristensen M, Vallès-Colomer M, et al. A low-gluten diet induces changes in the intestinal microbiome of healthy Danish adults. Nat Commun. (2018) 9:4630. doi: 10.1038/s41467-018-07019-x

PubMed Abstract | Crossref Full Text | Google Scholar

24. Schink M, Konturek PC, Tietz E, Dieterich W, Pinzer TC, Wirtz S. Microbial patterns in patients with histamine intolerance. J Physiol Pharmacol. (2018). doi: 10.26402/jpp.2018.4.09

PubMed Abstract | Crossref Full Text | Google Scholar

25. Sánchez-Pérez S, Comas-Basté O, Duelo A, Veciana-Nogués M, Berlanga M, Latorre-Moratalla M, et al. Intestinal dysbiosis in patients with histamine intolerance. Nutrients. (2022) 14:1774. doi: 10.3390/nu14091774

PubMed Abstract | Crossref Full Text | Google Scholar

26. Capurso L. Probiotics and prebiotics and food intolerance. Allergy. (2001) 56:125–6. doi: 10.1111/j.1398-9995.2001.00936.x

PubMed Abstract | Crossref Full Text | Google Scholar

27. Leis R, De Castro M, De Lamas C, Picáns R, Couce M. Effects of prebiotic and probiotic supplementation on lactase deficiency and lactose intolerance: A systematic review of controlled trials. Nutrients. (2020) 12:1487. doi: 10.3390/nu12051487

PubMed Abstract | Crossref Full Text | Google Scholar

28. Ardizzone A, Lanza M, Casili G, Campolo M, Paterniti I, Cuzzocrea S, et al. Efficacy of a novel therapeutic, based on natural ingredients and probiotics, in a murine model of multiple food intolerance and maldigestion. Nutrients. (2022) 14:2251. doi: 10.3390/nu14112251

PubMed Abstract | Crossref Full Text | Google Scholar

29. Ferrari E, Monzani R, Saverio V, Gagliardi M, Pańczyszyn E, Raia V, et al. Probiotics supplements reduce ER stress and gut inflammation associated with gliadin intake in a mouse model of gluten sensitivity. Nutrients. (2021) 13:1221. doi: 10.3390/nu13041221

PubMed Abstract | Crossref Full Text | Google Scholar

30. Besseling-van Der Vaart I, Heath MD, Guagnini F, Kramer MF. In vitro evidence for efficacy in food intolerance for the multispecies probiotic formulation Ecologic® Tolerance (Syngut™). BM. (2016) 7:111–8. doi: 10.3920/BM2015.0051

PubMed Abstract | Crossref Full Text | Google Scholar

31. Pusztai A, Ewen S, Grant G, Brown D, Stewart J, Peumans W, et al. Antinutritive effects of wheat-germ agglutinin and other N-acetylglucosamine-specific lectins. Br J Nutr. (1993) 70:313–21. doi: 10.1079/BJN19930124

PubMed Abstract | Crossref Full Text | Google Scholar

32. Zou R, Xu F, Wang Y, Duan M, Guo M, Zhang Q, et al. Changes in the gut microbiota of children with autism spectrum disorder. Autism Res. (2020) 13:1614–25. doi: 10.1002/aur.2358

PubMed Abstract | Crossref Full Text | Google Scholar

33. Zhi N, Chang X, Zha L, Zhang K, Wang J, Gui S. Platycodonis radix polysaccharides suppress progression of high-fat-induced obesity through modulation of intestinal microbiota and metabolites. Phytomedicine. (2025) 166:156653. doi: 10.1016/j.phymed.2025.156653

PubMed Abstract | Crossref Full Text | Google Scholar

34. Wang Q, Yu L, Campbell B, Milton J, Rhodes J. Identification of intact peanut lectin in peripheral venous blood. Lancet. (1998) 352:1831–2. doi: 10.1016/S0140-6736(05)79894-9

PubMed Abstract | Crossref Full Text | Google Scholar

35. Heilskov Rytter M, Andersen L, Houmann T, Bilenberg N, Hvolby A, Mølgaard C, et al. Diet in the treatment of ADHD in children—A systematic review of the literature. Nordic J Psychiatry. (2015) 69:1–18. doi: 10.3109/08039488.2014.921933

PubMed Abstract | Crossref Full Text | Google Scholar

36. Cagigal C, Silva T, Jesus M, Silva C. Does diet affect the symptoms of ADHD? CPB. (2019) 20:130–6. doi: 10.2174/1389201019666180925140733

PubMed Abstract | Crossref Full Text | Google Scholar

37. Nigg J, Lewis K, Edinger T, Falk M. Meta-analysis of attention-deficit/hyperactivity disorder or attention-deficit/hyperactivity disorder symptoms, restriction diet, and synthetic food color additives. J Am Acad Child Adolesc Psychiatry. (2012) 51: 86–97.e8. doi: 10.1016/j.jaac.2011.10.015

PubMed Abstract | Crossref Full Text | Google Scholar

38. Hamid R, Masood A. Dietary lectins as disease causing toxicants. Pak J Nutrit. (2009) 8:293–303. doi: 10.3923/pjn.2009.293.303

Crossref Full Text | Google Scholar

39. Mishra A, Behura A, Mawatwal S, Kumar A, Naik L, Mohanty S, et al. Structure-function and application of plant lectins in disease biology and immunity. Food Chem Toxicol. (2019) 134:110827. doi: 10.1016/j.fct.2019.110827

PubMed Abstract | Crossref Full Text | Google Scholar

40. Banwell J, Howard R, Kabir I, Costerton J. Bacterial overgrowth by indigenous microflora in the phytohemagglutinin-fed rat. Can J Microbiol. (1988) 34:1009–13. doi: 10.1139/m88-177

PubMed Abstract | Crossref Full Text | Google Scholar

41. Kim D, Wang Y, Jung H, Field R, Zhang X, Liu T, et al. type 2 immune circuit in the stomach controls mammalian adaptation to dietary chitin. Science. (2023) 381:1092–8. doi: 10.1126/science.add5649

PubMed Abstract | Crossref Full Text | Google Scholar

42. Bojarczuk A, Ska̧pska S, Mousavi Khaneghah A, Marszałek K. Health benefits of resistant starch: A review of the literature. J Funct Foods. (2022) 93:105094. doi: 10.1016/j.jff.2022.105094

Crossref Full Text | Google Scholar

43. Deng R, Yu S, Ruan X, Liu H, Zong G, Cheng P, et al. Capsaicin orchestrates metastasis in gastric cancer via modulating expression of TRPV1 channels and driving gut microbiota disorder. Cell Commun Signal. (2023) 21:364. doi: 10.1186/s12964-023-01265-3

PubMed Abstract | Crossref Full Text | Google Scholar

44. Porcari S, Benech N, Valles-Colomer M, Segata N, Gasbarrini A, Cammarota G, et al. Key determinants of success in fecal microbiota transplantation: From microbiome to clinic. Cell Host Microbe. (2023) 31:712–33. doi: 10.1016/j.chom.2023.03.020

PubMed Abstract | Crossref Full Text | Google Scholar

45. Chen Q, Wu C, Xu J, Ye C, Chen X, Tian H, et al. Donor-recipient intermicrobial interactions impact transfer of subspecies and fecal microbiota transplantation outcome. Cell Host Microbe. (2024) 32:349–365.e4. doi: 10.1016/j.chom.2024.01.013

PubMed Abstract | Crossref Full Text | Google Scholar

46. Sommer F, Anderson J, Bharti R, Raes J, Rosenstiel P. The resilience of the intestinal microbiota influences health and disease. Nat Rev Microbiol. (2017) 15:630–8. doi: 10.1038/nrmicro.2017.58

PubMed Abstract | Crossref Full Text | Google Scholar

47. Danne C, Rolhion N, Sokol H. Recipient factors in faecal microbiota transplantation: One stool does not fit all. Nat Rev Gastroenterol Hepatol. (2021) 18:503–13. doi: 10.1038/s41575-021-00441-5

PubMed Abstract | Crossref Full Text | Google Scholar

48. Grabauskas G, Gao J, Wu X, Zhou S, Turgeon D, Owyang C. WITHDRAWN: Gut microbiota alter visceral pain sensation and inflammation via modulation of synthesis of resolvin D1 in colonic tuft cells. Gastroenterology. (2022) S0016–5085:829–820. doi: 10.1053/j.gastro.2022.07.053

PubMed Abstract | Crossref Full Text | Google Scholar

49. Zhou Y, Zhang F, Mao L, Feng T, Wang K, Xu M, et al. Bifico relieves irritable bowel syndrome by regulating gut microbiota dysbiosis and inflammatory cytokines. Eur J Nutr. (2023) 62:139–55. doi: 10.1007/s00394-022-02958-0

PubMed Abstract | Crossref Full Text | Google Scholar

50. Lin C, Zeng T, Lu C, Li D, Liu Y, Li B, et al. Efficacy and safety of Bacteroides fragilis BF839 for pediatric autism spectrum disorder: A randomized clinical trial. Front Nutr. (2024) 11:1447059. doi: 10.3389/fnut.2024.1447059

PubMed Abstract | Crossref Full Text | Google Scholar

51. JanssenDuijghuijsen L, Looijesteijn E, Van Den Belt M, Gerhard B, Ziegler M, Ariens R, et al. Changes in gut microbiota and lactose intolerance symptoms before and after daily lactose supplementation in individuals with the lactase nonpersistent genotype. Am J Clin Nutr. (2024) 119:702–10. doi: 10.1016/j.ajcnut.2023.12.016

PubMed Abstract | Crossref Full Text | Google Scholar

52. Sarrabayrouse G, Bossard C, Chauvin J, Jarry A, Meurette G, Quévrain E, et al. CD4CD8αα lymphocytes, a novel human regulatory T cell subset induced by colonic bacteria and deficient in patients with inflammatory bowel disease. PLoS Biol. (2014) 12:e1001833. doi: 10.1371/journal.pbio.1001833

PubMed Abstract | Crossref Full Text | Google Scholar

53. Wan Y, Zuo T, Xu Z, Zhang F, Zhan H, Chan D, et al. Underdevelopment of the gut microbiota and bacteria species as non-invasive markers of prediction in children with autism spectrum disorder. Gut. (2022) 71:910–8. doi: 10.1136/gutjnl-2020-324015

PubMed Abstract | Crossref Full Text | Google Scholar

54. Hippe B, Remely M, Bartosiewicz N, Riedel M, Nichterl C, Schatz L, et al. Abundance and diversity of GI microbiota rather than IgG4 levels correlate with abdominal inconvenience and gut permeability in consumers claiming food intolerances. Emiddt. (2014) 14:67–75. doi: 10.2174/1871530314666140207103335

PubMed Abstract | Crossref Full Text | Google Scholar

55. Wang L, Christophersen C, Sorich M, Gerber J, Angley M, Conlon M. Increased abundance of Sutterella spp. and Ruminococcus torques in feces of children with autism spectrum disorder. Molecular Autism. (2013) 4:42. doi: 10.1186/2040-2392-4-42

PubMed Abstract | Crossref Full Text | Google Scholar

56. Fasano A, Chassaing B, Haller D, Flores Ventura E, Carmen-Collado M, Pastor N, et al. Microbiota during pregnancy and early life: Role in maternal-neonatal outcomes based on human evidence. Gut Microbes. (2024) 16:2392009. doi: 10.1080/19490976.2024.2392009

PubMed Abstract | Crossref Full Text | Google Scholar

57. Mann E, Lam Y, Uhlig H. Short-chain fatty acids: Linking diet, the microbiome and immunity. Nat Rev Immunol. (2024) 24:577–95. doi: 10.1038/s41577-024-01014-8

PubMed Abstract | Crossref Full Text | Google Scholar

58. Lackner S, Malcher V, Enko D, Mangge H, Holasek S, Schnedl W. Histamine-reduced diet and increase of serum diamine oxidase correlating to diet compliance in histamine intolerance. Eur J Clin Nutr. (2019) 73:102–4. doi: 10.1038/s41430-018-0260-5

PubMed Abstract | Crossref Full Text | Google Scholar

59. Arih K, Dordević N, Košnik M, Rijavec M. Evaluation of serum diamine oxidase as a diagnostic test for histamine intolerance. Nutrients. (2023) 15:4246. doi: 10.3390/nu15194246

PubMed Abstract | Crossref Full Text | Google Scholar

60. Manzotti G, Breda D, Di Gioacchino M, Burastero S. Serum diamine oxidase activity in patients with histamine intolerance. Int J Immunopathol Pharmacol. (2016) 29:105–11. doi: 10.1177/0394632015617170

PubMed Abstract | Crossref Full Text | Google Scholar

61. Beltrán-Ortiz C, Peralta T, Ramos V, Durán M, Behrens C, Maureira D, et al. Standardization of a colorimetric technique for determination of enzymatic activity of diamine oxidase (DAO) and its application in patients with clinical diagnosis of histamine intolerance. World Allergy Organ J. (2020) 13:100457. doi: 10.1016/j.waojou.2020.100457

PubMed Abstract | Crossref Full Text | Google Scholar

Keywords: food intolerance, fecal microbiota transplantation, gut microbiota, autism spectrum disorder, gastrointestinal symptoms

Citation: Huang Y, Huang J, Li Y, Xu T, Quan G, Xu P, Yang X, Liu Z and Xie W (2025) Therapeutic efficacy of fecal microbiota transplantation in severe food intolerance: a case report. Front. Nutr. 12:1594022. doi: 10.3389/fnut.2025.1594022

Received: 17 March 2025; Accepted: 18 April 2025;
Published: 19 May 2025.

Edited by:

Bowen Li, Southwest University, China

Reviewed by:

Fei Fan, China Academy of Chinese Medical Sciences, China
Himani Khanna, Jamia Hamdard Medical College, India

Copyright © 2025 Huang, Huang, Li, Xu, Quan, Xu, Yang, Liu and Xie. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Xiaoya Yang, eWFuZ3hpYW95YUBnendzLmVkdS5jbg==; Zhou Liu, bGl1emhvdUBnZG11LmVkdS5jbg==; Wenrui Xie, d2VucnVpeEBnZHB1LmVkdS5jbg==

These authors have contributed equally to this work

Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.