ORIGINAL RESEARCH article
Front. Nutr.
Sec. Nutrition and Microbes
Volume 12 - 2025 | doi: 10.3389/fnut.2025.1607365
Restoration of antibiotic associated diarrhea induced gut microbiota disorder by using Dictyophora indusiata water-insoluble polysaccharides in C57BL/6J mice
Provisionally accepted
- 1Southwest Medical University, Luzhou, China
- 2Sichuan Academy of Chinese Medicine Sciences, Chengdu, China
- 3The First Affiliated Hospital of Chongqing Medical and Pharmaceutical College, Chongqing, China
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Background: A healthy gut depends on a balance of commensal and probiotic bacteria, and prolonged and inappropriate clinical use of antibiotics can cause an imbalance in the gut flora, resulting in antibiotic-associated diarrhea (AAD). Macrofungal polysaccharides are rich in bioactivities and have attracted much attention for their good performance in anti-inflammatory, antioxidant, anti-obesity and anti-tumor properties. Therefore, we explored the role of Dictyophora indusiata water-insoluble polysaccharides (DIPY) in modulating the gut flora to improve AAD.Methods: We initially prepared the water-insoluble polysaccharides derived from Dictyophora indusiata. Subsequently, by comprehensively evaluating multiple parameters including the body weight, dietary patterns, cecal histomorphological characteristics, intestinal microbiota composition, concentrations of short-chain fatty acids (SCFAs), and the levels of inflammatory factors in the antibiotic-associated diarrhea (AAD) model animals, we delved into both the action mechanisms of these polysaccharides and their impacts on the intestinal flora and metabolites within C57BL/6J mice.Results: Our results showed that DIPY effectively ameliorated AAD in mice by modulating the intestinal flora, increasing microbial diversity, the Shannon and Ace index was significantly higher in the DIPY group than in the NR group after the DIPY intervention (P<0.001). Also, compared with the NR group, DIPY increasing the relative abundance of Parasutterella and Blautia, increasing the production of acetic acid (p<0.001), and decreasing the levels of LPS, MCP-1, TNF-α, and IL-6 (p<0.05) which attenuating inflammatory responses. Conclusion: This study demonstrated that DIPY has intestinal prebiotic function, which provides a basis for further development of functional products for the treatment of AAD.
Keywords: Polysaccharides, Antibioticassociated diarrhea, Gut Microbiota, Dictyophora indusiata, prebiotic
Received: 07 Apr 2025; Accepted: 18 Jun 2025.
Copyright: © 2025 Lai, Zhang, Xu, Guo, Zhou, Liu, Deng and Song. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Huiling Deng, The First Affiliated Hospital of Chongqing Medical and Pharmaceutical College, Chongqing, China
Can Song, Southwest Medical University, Luzhou, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.