The association between lutein and zeaxanthin intake and multi-level biological aging

Meiyi Tao¹Lin Zhang^2,3Caidi Jiang⁴Jia Xiang^5,6*Shipeng Chen^5,6*Songwen Tan^3,7*Shengli Sun^1*

• ¹Hunan Provincial People's Hospital (First Affiliated Hospital of Hunan Normal University), Changsha, Anhui Province, China
• ²Department of Epidemiology and Preventive Medicine, The School of Public Health and Preventive Medicine, Monash University, Melbourne, Australia
• ³Suzhou Industrial Park Monash Research Institute of Science and Technology, Suzhou, Liaoning Province, China
• ⁴Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, Hunan Province, China
• ⁵Hunan Pilot Free Trade Zone Global Cell Bank, Changsha, China
• ⁶Hunan Provincial Key Laboratory of the Research and Development of Novel Pharmaceutical Preparations, Changsha Medical University, Changsha, Hunan Province, China
• ⁷Monash University-Southeast University Joint Research Institute, Suzhou, China

This study investigates the potential association between lutein and zeaxanthin (LZ) intake, particularly lutein, and biological aging. The study aims to explore how LZ intake affects the biological aging progression, particularly in organs like the liver, kidneys, and cardiovascular system, and explore the potential mechanism of lutein as the main carotenoid mediating this effect.We analyzed biological aging using biological age calculations based on data from the NHANES 2007-2015 database. Various adjusted models were used to assess the relationship between LZ intake and aging phenotypes. Transcriptome analysis was conducted to explore the potential mechanisms underlying the anti-aging effects of lutein.A higher intake of LZ was associated with a slower biological aging rate (P < 0.01), observed in major organs such as the liver and kidneys, as well as the cardiovascular system. LZ intake showed a significant negative correlation with biological aging acceleration (P < 0.05). Enrichment analysis suggested that lutein's anti-aging effects might be mediated through telomere regulation and modulation of aging-related metabolic pathways. Additionally, lutein intake appeared to reduce pro-inflammatory Th1 cell abundance, further suggesting a potential anti-aging effect by suppressing inflammation. Sustained lutein intake also led to a decrease in the expression of aging phenotype-related molecules. However, in the evaluation of linear relationships, excessive lutein intake beyond a certain threshold may not yield additional benefits.Combined LZ intake is associated with attenuated multi-level biological aging (OR (95% CI): 0.93 (0.88, 0.93), P = 0.016) and high LZ intake significantly reduce the risk of all-cause death (P < 0.001), with lutein driving systemic effects via telomere regulation and inflammation suppression. These findings highlight lutein's translatable potential for aging interventions and provide insights for dietary strategies in aging health management.