ORIGINAL RESEARCH article
Front. Nutr.
Sec. Nutritional Immunology
Volume 12 - 2025 | doi: 10.3389/fnut.2025.1643051
This article is part of the Research TopicHealth Effects of Natural Compounds from PlantsView all 11 articles
Structural characterization and protective effects of polysaccharides isolated from Berberis dasystachya M. fruits on H 2 O 2 -induced oxidative damage in RINm5F pancreatic β-cells
Provisionally accepted
- Qinghai University, Xining, China
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Our previous study had demonstrated the hypoglycemic and antioxidant properties of crude polysaccharides derived from Berberis dasystachya M. (BDP). However, it lacks analysis of its polysaccharide structure or the mechanism underlying its antioxidant properties. The objective of this study is to examine the structural composition of the homogeneous polysaccharide BDP-I and elucidate its regulatory pathways and mechanisms in mitigating oxidative damage to pancreatic islet cells. Results showed that the molecular weight was 3.947 kDa, BDP-I contained 4 monosaccharide residues (Ara, Gal, Glc, and GalA ), primarily linked through →6)-β-D-GalAp-(1→ glycosidic bonds.Create an oxidative damage model by treating RIN-m5F pancreatic islet cells with 250 μmol/L H2O2 for 3 hours. BDP-I intervention at 0.0625-0.5 mg/mL significantly improved RIN-m5F cell survival rates, reduced ROS and TBARS levels, and increased SOD and CAT activities, demonstrating a dose-dependent effect. Metabolomics results indicate that BDP-I mitigated oxidative stress in pancreatic beta cells and reversed metabolic disturbances by regulating 46 potential biomarkers.Moreover, the BDP-I treatment of pancreatic islet cells modulates apoptosis-related genes, apoptotic pathway genes, and mitochondrial damage genes (iNOS, NF-κB) to intervene in cell apoptosis. These findings highlight the potential of BDP-I in the treatment of oxidative stress, and provide insights for the development of body targeted antioxidant therapy.
Keywords: Berberis dasystachya M., Metabolomics, Oxidative Stress, Pancreatic β-cells, Polysaccharides, structural characterization
Received: 07 Jun 2025; Accepted: 17 Jul 2025.
Copyright: © 2025 Lu, Han and Deng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Lijuan Han, Qinghai University, Xining, China
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