METHODS article
Front. Nutr.
Sec. Food Chemistry
Volume 12 - 2025 | doi: 10.3389/fnut.2025.1661855
Determination of α-Mannan Content in Composite Yeast Cultures by Enzymatic Hydrolysis HPLC-UV Method
Provisionally accepted
Xiaojie Zhang1
Lan Yang1Haifei Lian2Zixuan Xu1Zichen Yun1Xiaohui Xu1
Shixiong Liu1Qifei Luo1Majigsuren Zolzaya3Dacheng Liu1*- 1Inner Mongolia Agricultural University College of Veterinary Medicine, Hohhot, China
- 2Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot, China
- 3Mongolian University of Life Sciences, Ulaanbaatar, Mongolia
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
ABSTRACT: Background: Composite yeast culture (CYC) serves as an important functional feed additive. The content of its active ingredient, α-mannan, directly affects product quality and efficacy. However, due to the complex matrix of CYC, establishing a highly specific and accurate quantitative method for α-mannan remains a challenge. Methods: This study aimes to establish an analytical method based on enzymatic hydrolysis combined with high-performance liquid chromatography equipped with an ultraviolet detector (HPLC-UV) for the quantification of α-mannan in CYC. The method involved the specific enzymatic hydrolysis of α-mannan in the sample using α-mannosidase, followed by the release of mannose, which was then derivatized with 1-phenyl-3-methyl-5-pyrazolone (PMP) and analyzed by HPLC-UV. The actual content of α-mannan was calculated using a formula. Results: Method validation results showed that mannose exhibited good linearity within the concentration range of 0.5~400 μg/mL, with a correlation coefficient of 0.99998. The limit of detection (LOD) and limit of quantification (LOQ) were 0.063 mg/L and 0.208 mg/L, respectively. The average recovery rate was 88.020%–94.204%, with a relative standard deviation (RSD) ranging from 0.702% to 2.259%, indicating that the method has high accuracy and good precision. Conclusion: This study successfully established an efficient and reliable HPLC-UV detection method suitable for the specific quantitative analysis of α-mannan in CYC. The method exhibited good reproducibility and operational feasibility, providing robust technical support for quality control of CYC products and related functional component research.
Keywords: composite yeast culture, α-Mannan, α-mannosidase, Enzymatic hydrolysis, high-performance liquid chromatography
Received: 08 Jul 2025; Accepted: 12 Sep 2025.
Copyright: © 2025 Zhang, Yang, Lian, Xu, Yun, Xu, Liu, Luo, Zolzaya and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Dacheng Liu, donnyspets.cherry@outlook.com
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.