Abstract
Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder characterized by hyperandrogenism, anovulation, and insulin resistance (IR). Recent evidence suggests that gut microbiota (GM) dysbiosis contributes to PCOS pathophysiology, connecting metabolic, immune, and hormonal disturbances. Reduced microbial diversity, depletion of short-chain fatty acid (SCFA)-producing bacteria, and enrichment of endotoxin-producing taxa disrupt intestinal barrier integrity, promote low-grade inflammation, and aggravate IR, thereby fueling a vicious cycle of hyperinsulinemia and hyperandrogenism. Traditional Chinese medicine (TCM) has shown unique advantages in modulating GM and alleviating PCOS-IR. Herbal formulas, active compounds (e.g., berberine), acupuncture, and dietary therapies such as inulin, quinoa, and flaxseed oil restore microbial balance, enhance SCFA production, regulate bile acid metabolism, and strengthen gut barrier function. These effects mitigate endotoxemia, suppress chronic inflammation, and improve insulin sensitivity. This review summarizes advances in understanding the role of GM in PCOS-IR and emphasizes TCM as a promising microbiota-targeted therapeutic approach.
1 Introduction
PCOS is a common gynecological and endocrine disorder that involves both reproductive and metabolic dysfunctions. It affects approximately 5 to 20% of women of reproductive age worldwide and is recognized as a leading cause of anovulatory infertility (, ). The main characteristics of PCOS include ovulatory dysfunction, hyperandrogenism, and polycystic ovaries, and it is often accompanied by IR (). IR is present in an estimated 50 to 70% of women with PCOS and is associated with a range of adverse outcomes (, ). In the short term, it increases the risk of obesity, gestational diabetes and miscarriage, while in the long term, it contributes to the development of hyperlipidemia, type 2 diabetes mellitus (T2DM), metabolic syndrome, and cardiovascular disease (, ). These complications not only threaten the physical and mental health of patients but also complicate clinical treatment. Therefore, a comprehensive understanding of the mechanisms underlying insulin resistance in PCOS is essential for developing effective treatment strategies and enhancing both metabolic and reproductive outcomes for women affected by PCOS.
Recent studies have highlighted the crucial role of GM in the development of IR and PCOS (, ). As a symbiotic microorganism colonized in the human intestine, GM plays an essential role in regulating the host’s metabolic, immune, and endocrine functions (). Under healthy conditions, a dynamic balance exists between GM and the host, sustaining intestinal homeostasis. However, women with PCOS exhibit gut microbial dysbiosis, characterized by a decrease in beneficial probiotics and an increase in pathogens in the GM (, ). The imbalance of microbes undermines the integrity of the gut barrier, increasing gut permeability and allowing the bacterial endotoxin lipopolysaccharide (LPS) to enter the systemic circulation; the presence of LPS in the bloodstream activates the host’s immune response and pro-inflammatory signaling pathways, which interfere with insulin receptor function and promote the development of IR (, ). Additionally, GM and its metabolites influence metabolic regulation by stimulating the secretion of brain-gut peptides, promoting pancreatic β-cell proliferation, and reducing insulin sensitivity (). These effects result in compensatory hyperinsulinemia, further exacerbating IR. Therefore, maintaining a healthy and balanced GM is essential for mitigating insulin resistance and associated metabolic disturbances in PCOS.
Globally, the treatment of PCOS mainly focuses on conventional therapies like oral contraceptives, insulin sensitizers, and ovulation-inducing agents. However, these options are often limited by side effects, costs, and long-term safety concerns. As a result, more patients with PCOS are turning to complementary and alternative medicine (CAM), especially traditional Chinese medicine (TCM) such as herbs, acupuncture, and dietary supplements (). TCM, a major branch of CAM, has been practiced for thousands of years and is widely used in treating female reproductive disorders, including PCOS (, ). Chinese guideline for diagnosis and management of PCOS includes TCM as an auxiliary treatment method. Accumulating evidence suggests that certain individual herbs and herbal formulas containing multiple bioactive compounds have the potential to regulate menstruation, stimulate ovulation, reduce inflammation, and alleviate metabolic dysfunction (). Importantly, they have been shown to exert their therapeutic effects possibly through modulating the GM (). These oral herbal medicines interact directly with the GM, altering microbial composition and boosting the production of beneficial metabolites like short-chain fatty acids (SCFAs), which are crucial for maintaining metabolic balance and insulin sensitivity (). Therefore, TCM offers a promising complementary approach to managing PCOS-related insulin resistance. This review explores the current understanding of how TCM ameliorate PCOS-IR through GM modulation, providing a novel perspective for integrative therapeutic strategies.
2 Interaction between PCOS and IR
2.1 The relationship of IR and HA
The interplay between IR and hyperandrogenism (HA) is central to the pathogenesis of PCOS, forming negative feedback that drives both metabolic and reproductive dysfunction. In women with PCOS, IR leads to impaired glucose uptake, resulting in compensatory hyperinsulinemia (HI), which stimulates androgen production by ovarian theca cells. It also suppresses hepatic synthesis of sex hormone-binding globulin (SHBG), thereby elevating circulating free testosterone levels. Additionally, HI promotes neuroendocrine disturbances by enhancing gonadotropin-releasing hormone (GnRH) expression and luteinizing hormone (LH) secretion, further increasing ovarian androgen production. Moreover, IR disrupts the hypothalamic–pituitary–adrenal (HPA) axis, increasing adrenocorticotropic hormone (ACTH) levels and adrenal androgen synthesis (). These mechanisms converge to exacerbate HA, which in turn contributes to worsening IR by promoting visceral adiposity, reducing adiponectin and GLUT4 expression, and impairing insulin-stimulated glucose uptake in skeletal muscle (). This pathological loop fosters a pro-inflammatory and lipotoxic state, characterized by enlarged, dysfunctional adipocytes and dysregulated adipokine secretion, marked by decreased insulin-sensitizing adiponectin and elevated levels of leptin, resistin, and chemerin. HI also directly alters ovarian granulosa cell function by prematurely upregulating LH receptors, leading to early differentiation, follicular arrest, and anovulation. Furthermore, hyperinsulinemia enhances cytochrome P450c17 activity and increases insulin-like growth factor-1 (IGF-1) bioavailability, further stimulating androgen biosynthesis (). These disturbances are not limited to reproduction; they contribute to adverse pregnancy outcomes such as miscarriage and gestational diabetes, and promote the development of metabolic complications. Nevertheless, current evidence suggests that anti-IR treatment can decrease circulating levels of androgens and alleviate the phenotypes of PCOS () (Figure 1).
Figure 1
2.2 IR and metabolic dysfunction in PCOS
Compensatory hyperinsulinemia driven by IR not only promotes androgen excess but also leads to metabolic disturbances such as dysglycemia and dyslipidemia. Women with PCOS have approximately a threefold increased risk of developing impaired glucose tolerance (IGT), T2DM, and gestational diabetes mellitus (GDM) (, ). Lipid abnormalities are also prevalent in PCOS, with a characteristic profile of elevated triglycerides, reduced high-density lipoprotein (HDL) cholesterol, and increased levels of low-density lipoprotein (LDL) particles, changes that elevate cardiovascular risk (). Furthermore, metabolic dysfunction-associated steatotic liver disease (MASLD) is increasingly recognized in PCOS and is driven by androgen excess, IR, and enhanced lipolysis, leading to hepatic fat accumulation (). Accordingly, early intervention can alleviate the long-term health and economic burdens of patients with PCOS.
3 GM dysbiosis promotes PCOS-IR
3.1 GM and PCOS-IR
The GM is essential in preserving immune, metabolic, and endocrine homeostasis (). The most dominant bacterial phyla in the gut include Firmicutes and Bacteroidetes, which constitute about 90% of the gut microbiome. Within Firmicutes, the genus Clostridium is highly dominant, while Bacteroides and Prevotella are the major representatives of Bacteroidetes (). Other key phyla include Actinobacteria, mainly represented by Bifidobacterium, as well as smaller proportions of Proteobacteria, Fusobacteria, and Verrucomicrobia (). Under physiological conditions, these microbiomes maintain intestinal barrier integrity, modulate systemic inflammation, and support metabolic homeostasis through their close communication with intestinal epithelial and immune cells. The disruption of this delicate balance, termed dysbiosis, which is characterized by reduced microbial diversity, an imbalance in microbial composition, and compromised gut barrier function, has been implicated in the pathogenesis of various metabolic disorders (, , ).
Recent evidence suggests that PCOS is possibly related to dysbiosis, one of the primary features of dysbiosis is the reduction of microbial richness and diversity. GM diversity is a critical marker of intestinal and systemic health, influencing metabolism, immune balance, and inflammation (). It is commonly assessed using alpha (α) diversity, which reflects the richness and evenness of species within a sample, and beta (β) diversity, which measures compositional differences across individuals. In women with PCOS, several studies have reported a reduction in both α- and β-diversity, particularly in cases with IR (, ). In both clinical studies and animal models (e.g., letrozole-induced PCOS mice), decreased α- and β-diversity have been linked to altered gut microbial compositions, including a higher Firmicutes-to-Bacteroidetes ratio and increased abundance of pro-inflammatory gram-negative bacteria such as Escherichia, Shigella, and Bacteroides (, ). Inversely, some studies report no significant change in α-diversity in PCOS patients with normal BMI, while others suggest that observed changes are more closely tied to obesity or hyperandrogenism than to PCOS itself (, ). Factors such as diagnostic criteria, BMI, sex hormones, race, geography, eating habits, and host genetics may contribute to these discrepancies (, ). Overall, while dysbiosis is evident in PCOS, the extent of microbial diversity changes remains controversial.
Clinical studies consistently report alterations in microbial composition, characterized by the enrichment of pro-inflammatory and pathogenic taxa and depletion of beneficial commensals. A meta-analysis of 28 studies revealed a consistent enrichment of Bacteroides, Parabacteroides, Fusobacterium, and Escherichia/Shigella, and a reduction in Lachnospira and Prevotella, suggesting a shift toward a pro-inflammatory microbiome (). Further evidence associated these microbial shifts with reduced gut–brain peptides (serotonin, ghrelin, and PYY), increased testosterone, and altered BMI (). Elevated GABA-producing bacteria such as Parabacteroides distasonis, Bacteroides fragilis, and Escherichia coli were also found to correlate with elevated LH and LH/FSH ratios (). In obese adolescent girls with PCOS, higher relative abundance of Actinobacteria and Streptococcaceae, along with reduced Bacteroidaceae, has been reported. Importantly, several studies have distinguished PCOS-IR from non-IR phenotypes (). Significantly higher levels of Rothia, Enterococcus, Ruminococcus, and Bacteroidaceae, together with reduced Prevotellaceae, were observed in PCOS-IR patients, correlating with IR, inflammation, and hormonal disruption. Supporting these findings on the dysbiosis, animal models have confirmed causality (, ). Overgrowth of Bacteroides vulgatus induced IR and reproductive dysfunction in mice (), while microbiota depletion reversed IR and enhanced Farnesoid X receptor (FXR) signaling in PCOS models (). Other studies using letrozole- or DHEA-induced PCOS mice demonstrated increased Firmicutes and steroidogenic bacteria (e.g., Clostridiaceae, Nocardiaceae), alongside decreased beneficial taxa such as Akkermansia, Turicibacter, and Clostridium sensu stricto (, , ). Collectively, these findings underscore that gut microbial alterations in PCOS and PCOS-IR are closely linked to metabolic, endocrine, and inflammatory disruptions, reinforcing the GM as a potential therapeutic target (Table 1).
Table 1
| Investigation | Human/PCOS-like model | GM changes | Key Associations |
|---|---|---|---|
| Liang et al. () | Human (20 PCOS, 20 controls) | ↑ Parabacteroides distasonis, Bacteroides fragilis, E. coli | Positively correlated with LH levels and LH: FSH ratios |
| Li et al. () | Meta-analysis (28 studies; 1,022 PCOS, 928 controls) | ↓ Lachnospira, Prevotella; ↑ Bacteroides, Parabacteroides, Lactobacillus, Fusobacterium, Escherichia/Shigella | Indicative of pro-inflammatory dysbiosis |
| Liu et al. () | Human (33 PCOS, 15 controls) | ↑ Bacteroides, Escherichia/Shigella, Streptococcus; ↓ Akkermansia, Ruminococcaceae | Altered with BMI, testosterone, and decreased ghrelin and PYY |
| Jobira et al. () | Obese adolescents (58 total) | ↑ Actinobacteria, Streptococcaceae; ↓ Bacteroidetes, Bacteroidaceae, Porphyromonadaceae | PCOS-related changes in phylum/family levels in obese adolescents |
| Rodriguez Paris et al. () | DHT-induced PCOS-like mice | ↓ Bacteroides acidifaciens | Inversely associated with obesity |
| Qi et al. () | Human and mice | ↑ Bacteroides vulgatus | Promoted IR, altered bile acid metabolism, reduced IL-22, and induced infertility |
| Kelley et al. () | Letrozole-induced PCOS mice | ↓ Bacteroidetes; ↑ Firmicutes | Time-dependent changes associated with metabolic disturbances |
| Sherman et al. () | PCOS-like rats | ↑ Nocardiaceae, Clostridiaceae; ↓ Akkermansia, Bacteroides, Lactobacillus, Clostridium | Enriched for steroid hormone-related bacteria |
| Han et al. () | DHEA-induced PCOS-like rats | ↓ Turicibacter, Clostridium sensu stricto | Associated with glucose metabolism and fiber response |
| He et al. () | Human (PCOS-IR, PCOS-NIR, controls) | ↑ Rothia, Ruminococcus, Enterococcus; ↓ Prevotella | Linked to IR, blood pressure, waist/hip circumference |
| Zeng et al. () | Human (25 total) | ↑ Bacteroidaceae; ↓ Prevotellaceae | Abundance correlated with IR, inflammation, and hormone levels |
| Yang et al. () | Human and mice | ↑ Bacteroides | Gut microbiota removal reversed IR and upregulated FXR/FGF15 expression |
Investigations on regulating GM composition in PCOS.
3.2 The LPS and damaged gut barrier promote IR in PCOS
It is well known that the pathogenesis and development of PCOS is closely related to chronic low-grade inflammation, one of the key drivers of which is LPS, a pro-inflammatory endotoxin derived from Gram-negative gut bacteria (, ). The gut barrier is destroyed due to the GM dysbiosis in PCOS patients, allowing the transfer of LPS into systemic blood circulation and inducing metabolic endotoxemia (). The dysbiosis characterized by an overgrowth of Gram-negative bacteria such as Bacteroidaceae, Escherichia coli, Desulfovibrio, and Burkholderia leads to increased LPS production in the gut (, ). Under normal conditions, tight junction proteins such as occludin and ZO-1 maintain the integrity of the intestinal mucosal barrier. However, dietary factors such as high saturated fat intake and low fiber consumption compromise barrier function, increase gut permeability, and allow LPS to translocate into the bloodstream, which may be an early factor in the development of inflammation and IR in humans and mice (, ). In PCOS, patients often exhibit decreased expression of occludin and ZO-1, resulting in a “leaky gut” and elevated circulating LPS levels (, ). Once in the bloodstream, LPS binds to LPS-binding protein (LBP) and is recognized by the CD14/Toll-like receptor 4 (TLR4) complex on immune cells and various tissues, including ovarian theca cells. This interaction activates MyD88-dependent signaling cascades, leading to nuclear factor-κB (NF-κB) activation and the release of pro-inflammatory cytokines such as TNF-α, IL-1β, and IL-6 (, ). These cytokines interfere with insulin receptor signaling by promoting suppressor of cytokine signaling-3 (SOCS-3) expression and serine phosphorylation of insulin receptor substrate-1 (IRS-1), ultimately impairing GLUT4-mediated glucose uptake and exacerbating IR (). For instance, TNF-α has been shown to cause IR by increasing serine phosphorylation on IRS-1 (). IL-6, notably elevated in PCOS, further inhibits insulin signaling and contributes to IR and ovarian dysfunction by disrupting follicular development (). Experimental models have demonstrated that high-fat diets elevate LPS levels and induce IR (). Notably, direct LPS injection elevates fasting glucose and insulin levels, confirming its pathogenic role (). Emerging evidence suggests that modulating GM composition and restoring gut barrier integrity, such as through probiotics and Chinese herbal medicine, may reduce LPS translocation and inflammatory signaling. Collectively, in patients with PCOS, GM dysbiosis leads to elevated LPS levels, which increase intestinal permeability, impair insulin receptor function, and trigger a persistent inflammatory response, driving the progression of the PCOS-IR phenotype.
3.3 The products of GM promote IR
3.3.1 Short-chain fatty acids (SCFAs)
SCFAs, predominantly acetate, propionate, butyrate, and valerate, are critical microbial metabolites produced through the fermentation of dietary fibers by GM, which plays a vital role in metabolic regulation (). Studies show that women with PCOS have lower levels of SCFA-producing bacteria such as Butyricimonas, Blautia, Coprococcus, and Faecalibacterium prausnitzii, leading to decreased SCFA levels, especially butyrate, which may contribute to IR (). SCFAs exert their effects via activation of G protein-coupled receptors (GPR41, GPR43, GPR109A) and free fatty acid receptors (FFAR2/3) expressed on intestinal epithelial cells, enteroendocrine cells, adipose tissue, and pancreatic β-cells (). This signaling promotes the secretion of gut hormones such as glucagon-like peptide-1 (GLP-1) and peptide YY (PYY), which improve insulin sensitivity and regulate energy homeostasis (). Clinical evidence indicates that women with PCOS exhibit lower fecal SCFA levels compared to controls, with reductions inversely associated with fasting insulin (). Zhang et al. reported significantly higher fecal SCFAs in healthy women than in those with PCOS, while dietary or probiotic interventions restoring SCFA production improved glycemic and lipid profiles (). Probiotic supplementation, including strains like Bifidobacterium, has been shown to restore SCFA production, improve GLP-1 secretion, and enhance glycemic control in PCOS patients (). In mouse models, butyrate supplementation not only prevented obesity and IR on a high-fat diet but also enhanced mitochondrial function and energy expenditure (). Collectively, reduced SCFA levels, especially butyrate, due to gut microbial dysbiosis appear to play a pivotal role in the development of IR in PCOS. Besides, SCFAs strengthen the intestinal barrier and reduce LPS-induced endotoxemia by inhibiting inflammatory pathways like NF-κB, suppressing pro-inflammatory cytokines (e.g., TNF-α, IL-1β), and increasing the expression of tight junction proteins (). Collectively, reduced SCFA levels due to gut microbial dysbiosis contribute to the development of IR in PCOS.
3.3.2 Bile acid (BAs)
BAs are not only critical for lipid digestion and absorption but also function as potent signaling molecules that regulate glucose and lipid metabolism, inflammation, and energy homeostasis (). Primary BAs, synthesized in the liver as cholic acid and chenodeoxycholic acid, undergo microbial transformations in the intestine by bacteria such as Lactobacillus, Bifidobacterium, and Bacteroides, generating secondary BAs including deoxycholic acid (DCA), lithocholic acid (LCA), and ursodeoxycholic acid (UDCA) (). In PCOS, gut microbiota dysbiosis disrupts this transformation, leading to reduced beneficial bile acids like glycodeoxycholic acid (GDCA) and tauroursodeoxycholic acid (TUDCA) (). For instance, Bacteroides vulgatus overgrowth increases bile salt hydrolase (bsh) gene abundance, lowering GDCA and TUDCA levels and inducing insulin resistance and hormonal imbalance. Supplementation with GDCA or TUDCA can restore ovarian and metabolic function by activating the BA–IL-22 signaling axis (). Besides, BAs enhance insulin sensitivity via two major receptors: FXR and Takeda G-protein receptor 5 (TGR5) (). FXR activation suppresses gluconeogenesis and promotes glycogen synthesis through the PI3K/AKT pathway, while TGR5 activation in intestinal endocrine cells increases GLP-1 secretion and regulates appetite via the gut–brain axis (). In PCOS, impaired FXR/TGR5 signaling due to dysbiosis contributes to IR, hyperlipidemia, and chronic low-grade inflammation.
3.4 Brain-gut peptides
Recent evidence suggests that the pathogenesis of PCOS involves the gut-brain axis, a bidirectional network connecting the gut microbiota, the enteric nervous system, and central neuroendocrine circuits. Brain-gut peptides such as GLP-1, PYY, serotonin, and ghrelin are important factors in this axis. A clinical report indicated that the levels of ghrelin and PYY in the plasma of PCOS patients are significantly reduced, which is associated with an increase in the abundance of Bacteroides, E. coli/Shigella, and a decrease in beneficial bacteria such as Akkermansia (). Ghrelin not only regulates appetite and energy balance but also influences hypothalamic gonadotropin-releasing hormone GnRH secretion, modulating LH release and ovarian function. Similarly, PYY and GLP-1 promote satiety, delay gastric emptying, and enhance insulin sensitivity; their reduction exacerbates metabolic dysfunction and hyperinsulinemia. Studies have shown that fasting and postprandial GLP-1 levels are lower in both lean and obese PCOS women compared to healthy controls, and this deficiency persists despite oral contraceptive treatment (). In addition, ghrelin/obestatin imbalance and leptin dysregulation have been associated with altered lipid intake and increased HOMA-IR, further linking dietary composition, microbiota, and hormonal imbalance (). Moreover, gut microbes can modulate brain–gut peptides through the vagus nerve and neurotransmitter production, including serotonin and GABA, influencing both appetite control and hypothalamic–pituitary–ovarian (HPO) axis activity. Collectively, diminished gut–brain peptide signaling driven by microbial dysbiosis and altered metabolite production contributes to the bidirectional loop between metabolic and reproductive dysfunction in PCOS.
In summary, gut dysbiosis promotes PCOS-related IR by disrupting intestinal barrier integrity, elevating LPS-induced inflammation, and altering microbial metabolites such as SCFAs and bile acids. These changes impair brain-gut axis signaling, reduce GLP-1 and PYY secretion, and disrupt hormone balance, forming a vicious cycle of metabolic inflammation and reproductive dysfunction in PCOS (Figure 2).
Figure 2
4 TCM as GM modulator in PCOS-IR
4.1 Herbal formulas
Accumulating evidence highlights Chinese herbal medicine as a promising therapeutic method for PCOS, particularly by targeting GM to alleviate IR and chronic inflammation. Both clinical studies and PCOS-like animal models demonstrate that herbal formulas remodel gut microbial communities, restore intestinal barrier integrity, and regulate host metabolic signaling (Table 2; Figure 3). For instance, the Bu Shen Hua Zhuo Formula (BSHZF) reduced hyperandrogenism, fasting glucose, and IR in letrozole-induced PCOS rats while restoring microbial α-diversity, enriching Lactobacillus and SCFA-producing bacteria, and suppressing the TLR4/NF-κB inflammatory pathway by lowering serum LPS (). Similarly, the Shaoyao-Gancao Decoction (SGD) alleviated hyperandrogenism, estrous cycle disruption, and ovarian inflammation by increasing beneficial bacteria such as Akkermansia, Blautia, and Butyricicoccus, reducing LPS-producing Proteobacteria, enhancing tight junction proteins, and inhibiting TLR4/NF-κB activation (). Moreover, SGD was shown to regulate BA-related microbes and the BA/FXR pathway, suggesting dual actions on microbial and metabolic signaling ().
Table 2
| CHM | Human/Model | GM changes | Signaling pathways | Metabolic/reproductive outcomes |
|---|---|---|---|---|
| Formulas | ||||
| Bu Shen Hua Zhuo Formula (BSHZF) | Letrozole-induced PCOS rats | ↑ Lactobacillus, SCFA-producing bacteria (Allobaculum, Bacteroides, Ruminococcaceae), ↓ Firmicutes | ↓ LPS, TLR4/NF-κB inhibition | ↓ Body weight, fasting glucose, insulin, testosterone; improved ovarian morphology |
| Shaoyao-Gancao Decoction (SGD) | Letrozole-induced PCOS rats | ↓ Firmicutes/Bacteroidetes ratio; ↓ Proteobacteria; ↑ Butyricicoccus, Coprococcus, Akkermansia, Blautia, Bacteroides | Enhanced tight junctions; ↓ TLR4/NF-κB | Improved estrous cycles, reduced hyperandrogenism, ↓ inflammation |
| SGD (extended) | PCOS rats (fecal transplant study) | Remodelled BA-related bacteria | BA/FXR pathway regulation | Ameliorated dyslipidemia, estrous dysfunction |
| Bailing Capsules (BL) | DHEA + high-fat diet PCOS mice | ↑ Akkermansia, ↓ Muribaculaceae | IRS1/PI3K/AKT activation; ↓ TLR4/MyD88/NF-κB | Improved IR, hormone balance, ovarian morphology; ↓ inflammation |
| Heqi San (HQS) | DHEA + high-fat diet PCOS mice | ↑ Bifidobacterium, Parasutterella; ↓ Lachnoclostridium | ↓ NF-κB, M1 macrophage polarization; anti-apoptotic signaling | Improved IR, reduced granulosa apoptosis, ↓ IL-6, TNF-α, ovarian protection |
| Yulin Tong Bu Formula (YLTB) | DHEA + high-fat diet PCOS mice; pseudo-sterile models | Restored gut microbial diversity; metabolites (ferulic acid, folic acid) linked to PCOS parameters | Microbiota–metabolite interactions | Improved glucose clearance, insulin sensitivity, and ovarian function |
| Fufang Zhenzhu Tiao Zhi (FTZ) | Letrozole-induced PCOS mice | Not specified (systemic modulation) | ↑ Adiponectin signaling | Restored estrous cycles, alleviated IR, improved ovarian morphology |
| Guizhi Fuling Wan (GFW) | Letrozole + high-fat diet PCOS rats | ↑ Alloprevotella, ↓ pro-inflammatory Lachnospiraceae, Ruminococcaceae | ↓ Inflammatory cytokines (TNF-α, IL-6, hs-CRP) | ↓ Fasting insulin, improved IR and ovarian histology |
| Modified Banxia Xiexin Decoction | PCOS-IR rats (letrozole + high-fat diet) | ↑ Akkermansia, Blautia, ↓ Clostridium_sensu_stricto_1 | Barrier repair, metabolic regulation | Improved IR, corrected glucose metabolism |
| Modified Cangfu Daotan Decoction (MCDD) | PCOS-IR rats (letrozole + high-fat diet) | Not specified | ↓ NF-κB/LCN-2; ↑ Insr/Irs-1/Glut4 | ↓ Body weight, restored estrous cycle, ↓ fatty liver, improved IR |
| Jiawei Qi Gong Wan | Clinical study in PCOS patients (phlegm-dampness syndrome) | ↑ Butyrate-producing bacteria, ↓ LPS-producing and pro-inflammatory taxa | Microbiota–inflammation interaction | Improved IR, BMI, hormone balance, menstrual regulation |
| Active compounds | ||||
| Berberine (BBR) | Clinical trials in PCOS patients; DHEA-induced PCOS rats | ↓ Firmicutes/Bacteroidetes ratio; modulated Romboutsia, Bacteroides, Clostridium | Regulated glucose and glutamine metabolism; modulated KEGG pathways (T2DM, ABC transporters); ↓ LPS | ↓ Fasting insulin, HOMA-IR, testosterone; ↓ waist circumference; improved IR and metabolic profile |
| Naringenin (Nar) | Letrozole-induced PCOS rats | ↑ Butyricimonas, Lachnospira, Coprococcus, Roseburia; ↓ Prevotella | ↑ Tight junction proteins (claudin-1, occludin); modulated AMPK, SIRT1/PGC-1α | ↓ Body weight; improved estrous cycles, ovarian morphology, IR, and hormone balance |
| Dendrobium officinale polysaccharide (DOP) | Letrozole-induced PCOS rats | ↑ Butyrate-producing bacteria; ↑ α-diversity | ↑ Butyrate and PYY; GPR41-mediated gut–brain–ovary axis | Improved ovarian morphology, estrous cycles, and endocrine function |
| Cordyceps polysaccharide (CP) | PCOS-like rats | ↓ Desulfovibrionaceae, Helicobacter (Gram-negative bacteria) | ↓ LPS translocation; inhibited TLR4/MyD88/NF-κB in liver and adipose; restored insulin signaling | ↓ IR, improved glucose-lipid metabolism, alleviated ovarian polycystic lesions |
| Astragalus polysaccharide (APS) | DHEA-induced PCOS mice | ↑ Rikenellaceae, Odoribacter, Marinifilaceae; ↓ Bacteroidota | Reduced oxidative stress; gut microbiota–metabolite correlations with glucose and lipids | ↓ IR, OS, dyslipidemia; improved reproductive function |
| Mangiferin | Letrozole + HFD-induced PCOS rats | Altered Firmicutes, Bacteroidota, Proteobacteria; ↑ Blautia, Coprococcus, Roseburia | Anti-apoptotic (↓ Caspase-3, Cytc); modulated inflammation/apoptosis signaling | Improved lipid/glucose metabolism, IR, hormone balance, ovarian function |
| Curcumin | DHEA-induced PCOS mice | Not specified; improved barrier integrity | ↑ Occludin, ZO-1; inhibited TLR4/MyD88/NF-κB; ↓ IL-6, TNF-α, LPS | ↓ Body weight, testosterone, LH/FSH; ↑ insulin sensitivity, E2; improved ovarian/colon histology |
Effects of CHM Formulas and active compounds on GM and PCOS-IR.
Figure 3
Several classical prescriptions also show potential in regulating PCOS-IR through GM. Guizhi Fuling Wan (GZFL) improved IR and inflammation by reshaping microbial composition, notably restoring Alloprevotella and reducing inflammatory taxa (). Modified Banxia Xiexin Decoction (BX) and Modified Cangfu Daotan Decoction (MCDD) attenuated hyperinsulinemia, reduced inflammatory cytokines, and modulated NF-κB and LCN-2 pathway (, ). Clinical evidence also supports these findings, Jiawei Qi Gong Wan (JQG) improved IR and endocrine dysfunction in PCOS patients with phlegm-dampness syndrome by increasing butyrate-producing bacteria, reducing LPS-producing species, and restoring microbial diversity ().
Other formulas exhibit complementary benefits. Bailing capsules (BL) improved insulin sensitivity and ovarian function in DHEA-induced PCOS mice by repairing gut barrier integrity, reducing systemic inflammation, and inhibiting TLR4/NF-κB activation, while enriching Akkermansia (). Heqi San (HQS) demonstrated anti-inflammatory effects by suppressing NF-κB activity, inhibiting macrophage M1 polarization, and preventing granulosa cell apoptosis, alongside enriching Bifidobacterium and Parasutterella (). Yulin Tong Bu formula (YLTB) corrected ovarian dysfunction and glucose intolerance, with metabolomic analysis identifying ferulic acid as a key microbiota-associated mediator (). Fufang Zhenzhu Tiao Zhi (FTZ) improved estrous cycle regularity and IR in letrozole-induced PCOS mice by upregulating adiponectin, supporting fat–ovary metabolic crosstalk (). These findings underscore that CHM formulas act through enhancing SCFA-producing bacteria, modulating BA metabolism, reinforcing intestinal barrier function, suppressing LPS-induced inflammation, and restoring host metabolic pathways.
4.2 Bioactive compounds of herbal medicine
Modern studies have shown that bioactive compounds derived from herbal medicine play a therapeutic role in PCOS-IR by regulating the GM and its related metabolic pathways (Table 2; Figure 3). Berberine, an isoquinoline alkaloid derived from Coptis and Phellodendron species, has attracted considerable attention for its glucose- and lipid-lowering properties, with multiple studies supporting its potential to alleviate IR in PCOS. Clinical trials revealed that 12 weeks of berberine reduced waist circumference, HOMA-IR, and metabolic parameters more effectively than placebo and even metformin (). Mechanistic studies suggest that berberine acts partly through reshaping the GM, enhancing SCFA producers, and regulating key metabolites such as glutamine and glucose, thereby influencing host energy metabolism and inflammatory pathways (). However, the therapeutic role of berberine remains controversial. While certain PCOS-like rodent models confirmed improvements in IR, sex hormone profiles, and ovarian morphology, another study reported that berberine reduced microbial diversity without ameliorating metabolic or reproductive phenotypes (). Such discrepancies may reflect differences in experimental models, treatment duration, or baseline microbiota composition.
Naringenin (Nar), a natural flavanone, has demonstrated substantial benefits in PCOS-like models. Nar restored estrous cycles, improved ovarian morphology, and attenuated hyperandrogenism while reducing body weight and improving IR. GM sequencing revealed Nar-induced enrichment of SCFA-producing bacteria (Butyricimonas, Lachnospira, Coprococcus, Roseburia), alongside upregulation of tight junction proteins (claudin-1, occludin) in the colon (). These findings suggest that Nar exerts metabolic and reproductive improvements through enhancing intestinal barrier integrity and SCFA-driven signaling pathways.
Polysaccharides are another class of herbal medicine active ingredients with GM-dependent effects. Dendrobium officinale polysaccharide (DOP) cannot be directly absorbed but is fermented into SCFAs, particularly butyrate, by gut microbes. In PCOS rats, DOP increased microbial diversity, enriched butyrate producers, and elevated butyrate and PYY levels, which mediated improvements in ovarian morphology and estrous cyclicity via a gut–brain–ovary axis (). Similarly, Cordyceps polysaccharide (CP) ameliorated glucose-lipid disturbances by reducing Gram-negative bacteria such as Desulfovibrionaceae and Helicobacter, thereby lowering gut-derived LPS translocation. This suppressed TLR4/MyD88/NF-κB activation in the liver and adipose tissue, restored insulin signaling, and alleviated ovarian polycystic changes (). Astragalus polysaccharide (APS) also improved IR, oxidative stress, and dyslipidemia in PCOS mice, while reshaping GM by enriching beneficial bacteria such as Odoribacter and Marinifilaceae ().
Other phytochemicals exhibit complementary effects. Mangiferin, a xanthone glycoside, ameliorated ovarian dysfunction, IR, and lipid abnormalities in PCOS rats while significantly altering gut microbial composition, increasing beneficial SCFA-producing genera (Blautia, Coprococcus, Roseburia). Transcriptomic analyses further suggested its regulation of apoptosis and inflammatory signaling (). Curcumin, a well-known polyphenol, demonstrated anti-inflammatory and barrier-protective effects in PCOS models. It reduced serum testosterone and LH/FSH ratios, improved insulin sensitivity, and attenuated ovarian and colonic histopathology. Mechanistically, curcumin increased occludin and ZO-1 expression while suppressing TLR4/MyD88/NF-κB activation and systemic proinflammatory cytokines, thereby reducing LPS-induced endotoxemia (). Another polyphenol, resveratrol, is widely used in the treatment of PCOS. Wang et al. showed that fecal microbiota transplantation (FMT) from resveratrol-treated donors significantly improved ovarian function and increased microbial diversity, characterized by elevated Firmicutes/Bacteroidetes ratios and higher relative abundance of Lactobacillus murinus and L. salivarius (). Thus, GM as a central mediator of CHM active ingredients in alleviating PCOS-IR. By restoring gut microbial balance, reducing LPS leakage, and enhancing beneficial metabolites, these CHM bioactive compounds attenuate IR, hyperandrogenism, and ovarian dysfunction.
4.3 Dietary therapy
Dietary therapy, as a fundamental aspect of TCM, is increasingly being utilized in the research of PCOS (Figure 3). Recent experimental studies highlight the therapeutic role of functional foods such as quinoa and flaxseed oil (FO). In PCOS-like rats, quinoa supplementation significantly improved estrous cycle regularity, reduced fasting insulin and HOMA-IR, and alleviated ovarian, pancreatic, and intestinal pathology (). Besides, quinoa restored autophagy and PI3K/AKT/mTOR signaling in ovarian tissue, reinforced intestinal barrier integrity via upregulation of tight junction proteins, and shifted GM composition by enriching Lactobacillus, Bacteroides, and Oscillospira while reducing Prevotella and the Firmicutes/Bacteroidetes ratio (). These microbial and metabolic improvements were closely correlated with reductions in hyperandrogenism and improved reproductive outcomes. Similarly, flaxseed oil, rich in α-linolenic acid, exerted broad benefits in letrozole-induced PCOS rats (). FO corrected sex hormone imbalances, reduced body weight and dyslipidemia, and ameliorated IR. Anti-inflammatory effects were evident through reductions in plasma and ovarian IL-1β, TNF-α, and MCP-1, alongside increases in IL-10. Importantly, FO supplementation enriched beneficial microbes including Lactobacillus, Bifidobacterium, and Faecalibacterium, while reducing Proteobacteria and Streptococcus ().
Clinical evidence further supports the role of diet in PCOS pathophysiology. Meta-analyses reveal that women with PCOS consume significantly less dietary fiber than controls, a deficiency associated with greater adiposity, IR, and impaired glucose tolerance (). Dietary fiber fermentation by gut microbes yields SCFAs, which regulate host metabolism, immune homeostasis, and gut barrier integrity. Inadequate fiber intake may reduce SCFA production, exacerbating PCOS metabolic disturbances (). Integrating functional foods rich in fiber and unsaturated fatty acids may therefore represent a cost-effective, sustainable adjunct to conventional PCOS management.
4.4 Probiotics, prebiotics, and synbiotics
Prebiotics are organic substances that are not digested and absorbed by the host but can selectively promote the metabolism and proliferation of beneficial bacteria (). Common prebiotics include cellulose, polysaccharides, chitosan, and polyphenols. Inulin, a fermentable dietary fiber that enhances SCFA production, improves microbial diversity, and mitigates systemic inflammation. Clinical trials have shown that inulin supplementation in PCOS women reduced body mass, hyperandrogenism, and IR while lowering inflammatory cytokines (TNF-α, IL-1β, IL-6, MCP-1) (). Other studies in letrozole- or DHEA-induced PCOS mice confirmed that inulin increased SCFA production, restored estrous cycles, reduced testosterone, and suppressed ovarian inflammation via downregulation of LPS-TLR4 signaling (, ). Importantly, FMT from inulin-treated patients improved insulin sensitivity, lipid accumulation, and reproductive outcomes in antibiotic-treated mice ().
Synbiotics, which combine probiotics and prebiotics, have demonstrated stronger effects than either alone. Usually comes as a supplement in pharmaceutical form of juice and capsules. In PCOS mouse models, inulin-enriched synbiotic yogurt restored estrous cyclicity, improved ovarian morphology, and enhanced IL-22 secretion while shifting microbial composition toward Lactobacillus, Bifidobacterium, and Akkermansia, with concurrent modulation of bile acid metabolism (). Clinical studies further support these findings: randomized trials revealed that probiotic and synbiotic supplementation for 8–12 weeks significantly improved HOMA-IR, fasting glucose, lipid profiles, and hormonal balance (). The meta-analysis confirmed that synbiotics exert the most pronounced improvements in metabolic and endocrine outcomes, though variations in probiotic strains, dosing, and trial designs limit standardization ().
Probiotics are live microorganisms that confer health benefits by restoring microbial balance, enhancing gut barrier integrity, and modulating host immunity—particularly Bifidobacterium and Lactobacillus species. Specific bacterial species like Bifidobacterium lactis V9 supplementation in PCOS patients reduced LH/FSH ratios and increased SCFA levels, with clinical efficacy linked to successful gut colonization (). In DHT-induced PCOS mice, Bifidobacterium longum BL21 supplementation enhanced ovarian function, improved glucose tolerance, and reduced inflammatory cytokines while enriching beneficial microbiota (94). Similarly, Lactobacillus strains alleviated hyperandrogenism, restored estrous cycles, and improved ovarian morphology in letrozole-induced PCOS models, highlighting the gut–brain–ovary axis as a potential regulatory pathway (95). Therefore, these interventions not only improve insulin sensitivity and metabolic health but also alleviate hyperandrogenism and ovarian dysfunction, highlighting their dual impact on both reproductive and metabolic outcomes. However, clinical evidence remains limited by small sample sizes and short intervention durations, necessitating larger multicenter trials to establish standardized protocols (Figure 3).
4.5 Acupuncture
Acupuncture, a cornerstone of TCM, has gained attention as a non-pharmacological intervention for PCOS and IR. In animal models, electroacupuncture (EA) improved estrous cyclicity, reduced visceral adiposity, and enhanced glucose tolerance in dihydrotestosterone (DHT)-induced PCOS rats. These benefits were associated with shifts in microbial taxa, notably reduced Prevotella and altered Tenericutes abundance (96). Human studies provide more interesting insights. A randomized trial combining acupuncture with clomiphene in obese PCOS patients demonstrated greater reductions in LH/FSH ratios and improved IR compared with clomiphene alone, alongside compositional changes in GM, including increased Agathobacter faecis and decreased Erysipelatoclostridium and Streptococcus species. These microbial shifts may contribute to improvements in hormone balance and metabolism (97). However, large-scale trials report mixed outcomes: Wen et al. found that acupuncture was less effective than metformin in reducing HOMA-IR, though it showed advantages in glucose metabolism and fewer gastrointestinal side effects. Such findings demonstrate its potential as a low-risk adjunct therapy, especially in patients intolerant to pharmacologic agents (98) (Figure 3).
5 Shortcomings and future prospection
Although TCMs show considerable promise as modulators of the GM in alleviating IR in PCOS, current evidence is limited by several shortcomings that warrant critical attention. Most clinical studies are small, single-center trials with short intervention durations and heterogeneous diagnostic criteria, making it difficult to generalize findings or establish standardized treatment regimens. While some clinical trials, such as those investigating berberine, demonstrate significant improvements in IR and metabolic parameters, contradictory findings in animal models highlight the complexity of herbal medicine–microbiota–host interactions and the need for greater mechanistic clarity. Moreover, the lack of long-term safety evaluations and rigorous quality control in herbal preparation, standardization, and bioactive compound identification poses significant challenges to reproducibility and clinical translation. Variations in formulation, dosage, and preparation methods further complicate the interpretation of therapeutic outcomes and hinder cross-study comparisons. Furthermore, most existing studies examine single herbs or isolated compounds, whereas traditional Chinese medicine typically employs multi-herb prescriptions with synergistic interactions that remain poorly characterized. Future research should integrate multi-omics technologies and artificial intelligence (AI)-driven analytical models to identify active components, predict host–microbiota interactions, and optimize individualized therapeutic strategies, and conduct well-designed, large-scale, multicenter randomized clinical trials with standardized diagnostic criteria and safety assessments to make TCM a safe, effective, and evidence-based strategy for managing PCOS and its metabolic dysfunctions.
6 Conclusion
PCOS is a multifactorial disorder in which IR and hyperandrogenism form a vicious cycle driving metabolic and reproductive dysfunction. Increasing evidence indicate that GM dysbiosis as a pivotal mediator of these abnormalities through mechanisms involving impaired intestinal barrier integrity, endotoxemia, disrupted microbial metabolites such as SCFAs and BAs, and altered gut–brain–ovarian signaling. Within this context, TCM emerges as a promising modulator of GM, capable of restoring microbial balance, reducing inflammation, and improving IR. Preclinical and clinical studies have shown that herbal formulas, active ingredients, dietary fibers, synbiotic interventions, and acupuncture enhance the abundance of SCFA-producing bacteria, strengthen intestinal barrier function, and attenuate systemic and ovarian inflammation. Moreover, these interventions often exert synergistic effects on metabolic and endocrine pathways, linking microbiota regulation to improved reproductive outcomes. Here, we emphasize that TCM may offer an integrative therapeutic strategy to alleviate IR and improve long-term outcomes in PCOS by regulating the gut microbiome.
Statements
Author contributions
LY: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Software, Visualization, Writing – original draft. WY: Investigation, Software, Writing – original draft. QX: Investigation, Writing – original draft. JX: Investigation, Writing – original draft. YL: Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Writing – review & editing. JW: Funding acquisition, Project administration, Resources, Supervision, Writing – review & editing.
Funding
The author(s) declare that financial support was received for the research and/or publication of this article. This project was supported by the Joint Innovation Fund of Health Commission of Chengdu and Chengdu University of Traditional Chinese Medicine (File no. WXLH202403034), and the Young Talent Support Program by China Association of Chinese Medicine (2023–2025) (File no. CACM-2023-QNRC2-B03).
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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Abbreviations
PCOS, Polycystic ovary syndrome; IR, Insulin resistance; GM, Gut microbiota; SCFA, Short-chain fatty acid; LPS, Lipopolysaccharide; CAM, Complementary and alternative medicine; HA, Hyperandrogenism; HI, Hyperinsulinemia; HPA, Hypothalamic-pituitary-adrenal; IGF-1, Insulin-like growth factor-1; SHBG, Sex hormone-binding globulin; GLP-1, Glucagon-like peptide-1; PYY, Peptide YY; FXR, Farnesoid X receptor; TGR5, Takeda G-protein receptor 5; FMT, fecal microbiota transplantation.
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Summary
Keywords
polycystic ovary syndrome, gut microbiota, insulin resistance, traditional Chinese medicine, natural products, short-chain fatty acid
Citation
Yin L, Yang W, Xie Q, Xu J, Lan Y and Wu J (2025) The role of traditional Chinese medicine in modulating gut microbiota to alleviating insulin resistance in polycystic ovary syndrome. Front. Nutr. 12:1700612. doi: 10.3389/fnut.2025.1700612
Received
07 September 2025
Revised
21 October 2025
Accepted
05 November 2025
Published
25 November 2025
Volume
12 - 2025
Edited by
Xiaoang Li, Peking University Third Hospital, China
Reviewed by
Guoxin Huang, Macau University of Science and Technology, Macao SAR, China
Tingting Zhao, University of Macau, Macao SAR, China
Updates
Copyright
© 2025 Yin, Yang, Xie, Xu, Lan and Wu.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Ying Lan, lanyingtcm@163.com; Jie Wu, drwujie@163.com
† These authors have contributed equally to this work
Disclaimer
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