Multi-omics techniques revealing the mechanism of Polygonatum sibiricum Huangjiu in alleviating hyperlipidemia in mice

Jingzhang Geng¹Guotai Zhang¹Jianwei Dong^1*Honglei Tian¹Zaixiang Lou²

• ¹Shaanxi Province Key Laboratory of Bio-resources, College of bioscience and bioengineering, Shaanxi University of Technology,, Hanzhong, China
• ²School of Food Science and Technology, Jiangnan University, Wuxi, China

This study examined Polygonatum sibiricum Huangjiu's (PSHJ) ability to alleviate hyperlipidemia in mice brought by a high-fat diet, and the regulatory mechanism was studied via gut microbiome and fecal lipidomics. In the hyperlipidemia mice, PSHJ increased high-density lipoprotein cholesterol (HDL-C) while simultaneously decreasing serum levels of triglycerides (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C). Moreover, PSHJ was shown to repair liver, kidney, and small intestine damage in these mice. The regulating mechanism may be that Huangjiu's polysaccharides operate as prebiotics to improve the diversity of the gut microbiota, encourage the growth of functional bacteria that produce short-chain fatty acids (SCFAs), and indirectly raise SCFAs levels. The metabolic pathways of glycerophospholipids, glycerolipids, and fatty acids are then regulated by SCFAs and polysaccharides, promoting the breakdown metabolism of TG and diacylglycerol (DG) in hyperlipidemic mice. Overall, our research shows that PSHJ has active components that can alleviate hyperlipidemia, thereby laying a theoretical foundation for extracting of bioactive substances from Huangjiu for future medical or dietary use.