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Front. Chem., 09 November 2020 | https://doi.org/10.3389/fchem.2020.596479

Nature's Chemists: The Discovery and Engineering of Phytochemical Biosynthesis

  • Centre for Novel Agricultural Products, Department of Biology, University of York, York, United Kingdom

Plants produce a diverse array of natural products, many of which have high pharmaceutical value or therapeutic potential. However, these compounds often occur at low concentrations in uncultivated species. Producing phytochemicals in heterologous systems has the potential to address the bioavailability issues related to obtaining these molecules from their natural source. Plants are suitable heterologous systems for the production of valuable phytochemicals as they are autotrophic, derive energy and carbon from photosynthesis, and have similar cellular context to native producer plants. In this review we highlight the methods that are used to elucidate natural product biosynthetic pathways, including the approaches leading to proposing the sequence of enzymatic steps, selecting enzyme candidates and characterizing gene function. We will also discuss the advantages of using plant chasses as production platforms for high value phytochemicals. In addition, through this report we will assess the emerging metabolic engineering strategies that have been developed to enhance and optimize the production of natural and novel bioactive phytochemicals in heterologous plant systems.

Introduction

Plants have a remarkable capacity to produce a wide array of specialized metabolites (also referred to as secondary metabolites or natural products), to support their defense and ecological adaptation. These phytochemicals exhibit a variety of bioactivities and many of them are of considerable pharmaceutical importance. Based on their biosynthetic origins, plant secondary metabolites can be divided into three major groups: terpenoids, alkaloids, and phenolics.

Terpenoids or isoprenoids are a structurally diverse class of plant specialized metabolites. They are derived by the repetitive fusion of branched five-carbon isopentanes, usually referred to as isoprene units. Many terpenoids have biological activities and are utilized as valuable pharmaceuticals. The most renowned terpenoid-based drugs include the antimalarial medicine artemisinin (Artemisia annua) and the anticancer drug Taxol (Taxus brevifolia) (Figure 1A). Another major class of secondary metabolites are alkaloids, nitrogen containing low-molecular-weight compounds, typically derived from amino acids (Lichman, 2020). Numerous alkaloids (or their derivatives) are employed as high value drugs, such as vincristine and vinblastine (anticancer) from Madagascar periwinkle (Catharanthus roseus), camptothecin (anticancer) from Camptotheca acuminata and morphine (analgesic drug) from opium poppy (Figure 1B). Phenolics are a large and diverse group of aromatic compounds which include the tannins, phenylpropanoid, anthocyanin and lignan subgroups. Etoposide and teniposide are among the most pharmaceutically important polyphenols (Figure 1C). These compounds are derivatives of podophyllotoxin, a lignan from mayapple (Podophyllum peltatum), and are widely used in chemotherapies. Alongside alkaloids, terpenoids, and phenolics, plants also produce other types of secondary metabolites, such as polyketides, cyanogenic glycosides and glucosinolates, of which many have pharmaceutical relevance.

FIGURE 1
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Figure 1. Examples of plant specialized metabolites of pharmaceutical significance. (A) Terpenoids: artemisinin and taxol. Isoprene units are highlighted with colors. (B) Alkaloids: camptothecin, vinblastine, and vincristine with tryptophan precursor highlighted; morphine tyrosine precursors highlighted. (C) Phenolics: etoposide and teniposide with phenylpropanoid units highlighted.

Sourcing bioactive natural products from plants has many challenges. These metabolites are often found in low abundance or are produced in rare or slow-growing plant species, which limits the availability and accessibility of the active pharmaceutical agent and impacts market prices. In many cases, these molecules are very complex and are recalcitrant to chemical synthesis, as they contain many chiral centers or polycyclic rings (Nicolaou and Rigol, 2020). Even when synthesis is possible, it is not sustainable as it often relies on petrochemical feedstocks and/or environmentally unfriendly production processes (Lechner et al., 2016). This makes finding alternative sources for the supply of these valuable molecules necessary.

Increased access to phytochemicals can be achieved through a number of approaches. For instance, the biosynthetic capabilities of the natural producer may be enhanced through selective breeding programmes (Townsend et al., 2013) or through genetic modification and the creation of transgenic lines (Shen et al., 2018). Cell cultures may also enable greater control over the production of the compound of interest. For example, cambial meristematic cell cultures derived from Taxus cuspidata have been shown to produce high yields of the anticancer drug, paclitaxel (Taxol), while circumventing the obstacles routinely associated with the commercial growth of dedifferentiated plant cells (Lee et al., 2010).

Another alternative and increasingly popular approach is the transfer of biosynthetic pathways into heterologous systems such as plants (e.g., Nicotiana benthamiana) or microbes (e.g., Saccharomyces cerevisiae and Escherichia coli). The advantages of using microbes as production platforms for valuable chemicals include fast growth cycles, ease of genetic modification, and the fact that they offer a simplified product purification pipeline. The synthetic biology approaches used to produce plant metabolites in microbial organisms have been recently reviewed (Moses et al., 2017; Li et al., 2018). Plants, on the other hand, are photoautotrophs and do not require exogenous carbon sources, unlike typical microbial platforms. Plants also provide a cellular context similar to the native producer, which make them an attractive platform for the production of valuable chemicals.

In order to engineer valuable phytochemicals in microbe or plants, the first step is to uncover the biosynthetic pathway of the metabolite of interest. In this review we highlight the recent methods that have been used to elucidate natural product biosynthetic pathways, including the approaches leading to proposing the sequence of enzymatic steps, assigning enzyme family, as well as, gene function elucidation. We will also discuss the advantages of using plant chassis as a production platform for high value phytochemicals. In addition, through this report we will assess the emerging metabolic engineering strategies that have been developed to enhance and optimize the production of natural and novel bioactive phytochemicals in heterologous plant systems.

Discovery of Specialized Metabolic Pathways

Proposing a Biosynthetic Pathway

In order to identify the enzymes that underlie the biosynthesis of valuable plant natural products, it is first necessary to hypothesize a plausible sequence of enzyme catalyzed reactions that can lead from primary metabolism to the molecule of interest. Generally, biomimetic syntheses tend to use mild conditions and harness cascade-like reactions commonly found in biosynthetic pathways, hence providing clues for pathway elucidation (Yoder and Johnston, 2005).

If the metabolic pathway is incompletely described, isotope-labeling studies can be used to identify the unknown steps. Primary metabolites, such as amino acids, sugars, or even dioxygen and carbon dioxide, labeled with stable isotopes (e.g. 2H, 13C, 15N, and 18O), can be fed to the plant. The target specialized metabolite can then be assessed for isotope labeling by mass spectrometry (MS) or nuclear magnetic resonance (NMR) (Freund and Hegeman, 2017). This allows us to establish the identity of the starting material and identify its mode of incorporation. Strategically positioned isotopic labels can be used to probe the transformations and structural rearrangements that occur along the biosynthetic pathway.

For example, the incompletely described biosynthesis of camptothecin, a monoterpene indole alkaloid (MIA) from Camptotheca acuminata, was investigated using metabolite profiling and isotope labeling studies. The approach led to the identification of plausible intermediates for missing pathway steps and demonstrated that nearly all camptothecin pathway intermediates were present as multiple isomers (Sadre et al., 2016). Therefore, these metabolite focused strategies can lead to a hypothetical biosynthetic pathway made up of a set of chemically reasonable enzymatic conversions.

The next step is to assign possible enzyme classes to each hypothesized reaction based on characterized enzyme activities. For instance, a hydroxylation step will probably involve a cytochrome P450 enzyme or a 2-oxoglutarate dependent oxygenase (Mitchell and Weng, 2019), whilst a transfer of an amine group onto a carbonyl is likely to involve a PLP-dependent enzyme (Lee and Facchini, 2011). Once the hypothetical pathway has been populated with proposed enzyme classes, the gene discovery effort can begin.

Integrative Approaches for Gene Identification

The discovery of genes involved in the biosynthesis of phytochemicals typically requires the use of multi-omics technologies such as genomics, transcriptomics, proteomics, and metabolomics. Analysis of these data enable the identification of candidate genes that encode enzymes that catalyze biosynthetic steps (Figure 2).

FIGURE 2
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Figure 2. Overview of the approaches that can be employed to discover unknown enzymes in a biosynthetic pathway of interest. (A) Identification of candidate genes in plant material with difference in metabolites accumulation, through co-expression analysis or phylogenetic analysis to examine whether a gene clade is specific to the native plant producer. (B) Mining genomic data to look for genes that are physically localized in the vicinity of previously characterized enzymes (gene clusters). (C) Total protein purification and fractionation from the native plant material, followed by functional assays and proteomic identification of the active fraction.

The biosynthesis of natural products is regulated both during development and in response to various environmental stimuli. Transcriptomics can pinpoint differentially expressed genes across different types of tissues (or cells), different developmental stages or in elicitor-treated material. Candidate genes involved in biosynthesis can be identified through correlation of expression with metabolite accumulation, or through identification of co-expressed clusters in which genes involved in the same pathway share expression patterns across tissues (Figure 2A).

The hormone methyl jasmonate (MeJA) is known to elicit various species-specific specialized metabolic pathways. This feature has often been used to identify genes involved in the biosynthesis of various natural products. Cytochrome P450 (CYP728B70) catalyzing the oxidation step in triptolide biosynthesis, an abietane-type diterpenoid from Tripterygium wilfordii, was identified through an integrative gene prioritization approach (Tu et al., 2020). The prioritized candidate genes were highly expressed in MeJA-induced cells and/or in the root bark (main accumulation site of the diterpene), as well as, exhibited similar expression patterns to already characterized enzymes in the pathway; copalyl diphosphate synthase and miltiradiene synthase (Tu et al., 2020).

Various valuable plant natural products have been shown to accumulate in specific specialized structures, such as artemisinin in the glandular trichomes of Artemisia annua (Duke et al., 1994) and morphine in the laticifers of the aerial organs of opium poppy (Papaver somniferum) (Facchini and De Luca, 1995). Generating multi-omic data from these structures allows the identification of new biosynthetic enzymes involved in pathways of interest. For example, comparative proteomic analysis of trichomes and trichome-depleted leaves in catmint (Nepeta mussinii) led to the discovery of the unusual nepetalactol-related short-chain dehydrogenase enzymes (NEPS), which are enriched in the trichome and involved in the biosynthesis of volatile nepetalactones (Lichman et al., 2019).

Phylogenetic analysis can provide valuable information to further guide the candidate gene prioritization process. Genes involved in a biosynthetic pathway unique to a particular species are likely to be phylogenetically distinct. Therefore, building phylogenetic trees containing candidate genes and homologous genes from other species may reveal subclades unique to the producer plant. Key oxidases in limonoid biosynthesis in Melia azedarach; CYP71BQ5 and CYP71CD2 were identified through this strategy. These cytochrome P450 enzymes were found in a distinct subclade within the phylogenetic tree, comprising only Melia azedarach sequences, suggesting their involvement in this lineage-specific pathway (Hodgson et al., 2019).

Candidate gene selection may also be supplemented by mining whole genomes or partial genome sequences for genes physically localized in the vicinity of previously characterized enzymes (Figure 2B). It has been reported that genes involved in biosynthetic pathways of a number of natural products are organized in functional clusters within plant genomes (Roselli et al., 2017; Lichman et al., 2020; Liu et al., 2020). Features of plant biosynthetic gene clusters, how they form and how they are regulated have been recently reviewed (Nützmann et al., 2016, 2018). Another approach is total protein purification and fractionation, from the native plant material, followed by functional assays (Figure 2C). Protein fractions demonstrating the desired enzyme activity can be analyzed through protein-mass spectrometry and the identity of the enzyme determined by comparison with a predicted peptide database. The formation of thebaine, the first opiate alkaloid in the biosynthesis of codeine and morphine in opium poppy, can occur spontaneously from (7S)-salutaridinol 7-O-acetate. However, functional assays with total soluble protein isolated from opium poppy latex resulted in a 10-fold increase in the formation of thebaine. After protein fractionation and subjecting the active protein fraction to LC-MS/MS proteomics analysis, candidate genes were identified and were then tested through enzymatic assays, leading to the functional characterization of thebaine synthase (Chen et al., 2018).

Most of the aforementioned strategies, such as co-expression analysis, phylogenetics and genome mining, tend to be employed in an integrative way to allow efficient prioritization of candidate genes responsible for metabolic traits of interest. The next step is to verify the identity of the gene through functional assays.

Functional Characterization of Candidate Genes

Two types of experiments are typically required to ascribe a gene to a biosynthetic pathway: enzyme activity assays and reverse genetics gene function validation. The activity of the encoded enzyme must be characterized; this is typically achieved using recombinant expression. However, activity alone is not sufficient to determine in planta function. Here, reverse genetics approaches such as gene silencing are necessary to verify the role of the gene in a pathway.

Pathway Elucidation in Microbial Platforms

Recombinant protein expression in Escherichia coli has been extensively used for the enzymatic characterization of biosynthetic genes. The advantages of employing this prokaryotic organism include fast growth kinetics and well-established molecular tools. Protein expression in E. coli is often followed by enzyme purification and in vitro assays. This enables the assessment of the biochemical activities of the candidate enzymes outside the complex cellular context (Caputi et al., 2018; Torrens-Spence et al., 2018; Lichman et al., 2019; Kim et al., 2020) (Figure 3).

FIGURE 3
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Figure 3. Overview of the functional characterization methods that can be utilized to determine gene function; enzymatic assays, heterologous expression of candidate biosynthetic genes in microbial host (yeast cells), and heterologous expression in planta (Nicotiana benthamiana).

However, several enzyme families frequently involved in specialized metabolism in plants are membrane-associated, such as cytochrome P450s and membrane-bound prenyltransferases, or require specific eukaryotic-type post-translational modifications. For expression of these proteins, Saccharomyces cerevisiae is preferred over E. coli because of its eukaryotic cell architecture, including the availability of more suitable protein post-translational mechanisms. Microsomal fractions of yeast cells containing active recombinant proteins can be prepared and utilized for subsequent biochemical assays (Levsh et al., 2019).

Alternatively, it is possible to exploit endogenous yeast metabolism and examine the enzymatic activity in an in vivo context, without purification. This method relies on the available precursors and substrates within native yeast pathways (Figure 3). This approach may be preferred over in vitro assays if enzyme purification is challenging or higher throughput is required. If the substrate of interest is not available in yeast, an exogenously supplied substrate can be used to build the desired secondary metabolite (Tu et al., 2020). Another option is to engineer yeast strains through genome engineering (e.g., CRISPR or homologous recombination) to accumulate specific precursors that are not naturally present in yeast (Luo et al., 2019; Munakata et al., 2019), or to shutdown endogenous competitive branches that deplete the precursor of interest (Moses et al., 2014).

Pathway Elucidation in Nicotiana benthamiana

Nicotiana benthamiana is a practical heterologous expression system for plant natural product biosynthetic pathways (Figure 3). It is highly amenable to Agrobacterium-mediated transformation, allowing the transient expression of one or, simultaneously, multiple gene(s) of interest. This plant also provides a cellular context that is similar to the native producer plant. This characteristic allows the expressed proteins to be properly folded and targeted to the correct subcellular compartment, as well as provides a natural supply of chemical precursors; coenzymes, and cofactors.

Heterologous expression of candidate genes in N. benthama has led to the discovery of a variety of enzymes underlying the biosynthesis of diverse high value plant-derived compounds (Levsh et al., 2019; Pluskal et al., 2019). The plant can also be used to reconstitute whole pathways for preparation of high-value compounds. For example, eight genes from Gloriosa superba involved in the biosynthesis of the alkaloid colchicine were discovered using N. benthamiana as a screening tool. Subsequently, the newly discovered genes plus eight previously described genes were used to engineer N. benthamiana to synthesise N-formyldemecolcine, a colchicine precursor, from phenylalanine and tyrosine (Nett et al., 2020). The use of N. benthamiana for the heterologous production of whole pathways is discussed further in section Plants as Chasses for Production of High-Value Phytochemicals.

Virus Induced Gene Silencing

Virus-induced gene silencing (VIGS) is a reverse genetics approach commonly employed for the in vivo functional characterization of candidate biosynthetic genes (Moglia et al., 2016). VIGS provides direct evidence of the biological role of the gene through a loss-of-function mechanism. The system takes advantage of the plant's homology-dependent defense mechanisms in response to attack by viruses (Hileman et al., 2005). Inoculating the plant with a construct containing viral RNA and a fragment of the target gene will trigger the degradation or the inhibition of translation of the correspondent RNA, leading to the silencing of the gene of interest. The knock-down of specific genes through this system will reveal their involvement in the biosynthesis of the target natural product. The system was established and used in a variety of plants such as Madagascan periwinkle (Catharanthus roseus) (Qu et al., 2019) and opium poppy (Chen et al., 2018). However, a species-specific set up of the method is needed for each newly studied plant by, for instance, identifying suitable virus for the plant and efficient inoculation methods (Courdavault et al., 2020).

Plants as Chasses for the Production of High-Value Phytochemicals

Expressing genes in heterologous plant systems not only enables pathway elucidation and gene function determination, but also provides new opportunities to increase and diversify the production of high-value bioactive phytochemicals. As mentioned above, N. benthamiana represents a valuable tool for the heterologous production of phytochemicals. For example, 16 biosynthetic genes from Himalayan mayapple (Podophyllum hexandrum) were transferred to N. benthamiana to produce precursors of the chemotherapeutic drug etoposide at milligram-scale (Schultz et al., 2019).

A major advantage of using N. benthamina as a production platform is the rapidity of the process; no transgenic plants need to be generated. Turnaround times are comparable to microbial systems, with the detection of products possible a few days after agroinfiltration. The process can be scaled up using vacuum agroinfiltration to reach gram-scale production of phytochemicals (Reed et al., 2017).

Optimizing Plant-Based Production of Phytochemicals

Diverse strategies have been used to enhance and optimize the production of phytochemicals in planta. Among these strategies include overexpressing yield-boosting enzymes such as tHMGR (truncated 3-hydroxy-3-methylglutaryl-CoA reductase, key rate-limiting enzyme in mevalonate pathway) (Reed et al., 2017) or 1-deoxy-d-xylulose 5-phosphate synthase (DXS), the first committed MEP pathway enzyme (Brückner and Tissier, 2013). Another method that can potentially lead to increased production is the suppression of competitive pathways through inactivation of endogenous genes by virus-induced gene silencing (Hasan et al., 2014) or RNA interference (Cankar et al., 2015).

The biosynthesis of natural products in plants is highly compartmentalized with different steps of biosynthetic pathways occurring in different subcellular localization (Heinig et al., 2013). To enhance the production of phytochemicals of interest, an alternative method is to alter the subcellular location of heterologously expressed enzymes by addition, removal or modification of target peptides (Reed and Osbourn, 2018). Various studies have shown the potential of this strategy in enhancing the production, with targeting biosynthetic enzymes in different subcellular compartments. Dong et al. (2016) reported that the targeting of geraniol synthase (GES) from Valeriana officinalis in the chloroplasts of N. benthamina increased the production of GES products compared to the mitochondrial- and cytosolic-targeted GES.

Emerging Metabolic Engineering Strategies

The ability of plant specialized structures, such as glandular trichomes, to synthesize and store hydrophobic and toxic metabolites has the potential to make engineering the production of these metabolites in plants more advantageous (Huchelmann et al., 2017). Conditions in trichomes and other structures may even be vital for the formation of the target compound: the final oxidative steps in the biosynthesis of artemisinin are non-enzymatic and require the non-aqueous environment of the subapical cavity of glandular trichomes to proceed (Czechowski et al., 2016). These findings highlight the challenges of producing specific secondary metabolites in microbial platforms that lack this level of structural complexity.

In addition, engineering metabolic pathways in plants is sometimes hindered by toxicity issues and growth defects, due to the cytotoxicity of the engineered compounds or the depleted pools of precursors, necessary for central metabolism. Expressing these pathways in a cell-type specific way, by taking advantage of the separation of trichomes from the rest of the plant, might offer a solution for these issues (Tissier et al., 2012). Efforts to produce secondary metabolites in trichomes through the use of trichome-specific promoters have been reported, such as; taxadiene in N. sylvestris, and casbene (diterpene from Ricinus communis), in N. tabacum (Table 1) (Rontein et al., 2008; Tissier et al., 2012).

TABLE 1
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Table 1. Examples of engineered cellular/subcellular localization of phytochemical production in Nicotiana sp.

Engineering plastids in order to optimize the yields of valuable phytochemicals has been explored. Plastid engineering has various advantages over nuclear genome engineering including an efficient homologous recombination machinery, as well as the potential for greater gene expression levels (Bock, 2015; Boehm and Bock, 2019). To enhance the yield of artemisinic acid production (the precursor for the anti-malaria drug: artemisinin), a synthetic biology strategy has been developed combining plastome and nuclear transformation (Fuentes et al., 2016). The artemisinic acid metabolic pathway was introduced into the chloroplast genome of N. tabacum plants. Subsequently, enzymes known to affect the metabolic flux were introduced through nuclear transformation (Table 1). This strategy led to identifying plants that produce high amounts of artemisinic acid (more than 120 mg of per kg biomass) (Fuentes et al., 2016; Jensen and Scharff, 2019).

A newly emerging metabolic engineering strategy is engineering synthetic hydrophobic droplets within cells that enable the accumulation and storage of lipophilic compounds such as terpenoids. For example, the synthesis of lipid droplets in transient N. benthamiana system was enhanced through the ectopic production of a regulator of plastid fatty acid biosynthesis and a microalgal lipid droplet surface protein. Biosynthetic steps were anchored onto the surface of the lipid droplets and high-value sesqui- or diterpenoids were efficiently produced. These engineered lipid droplets may potentially facilitate terpenoid extraction from the plant, through “trapping” of the target molecules in the oil fraction (Table 1) (Sadre et al., 2019). Another study demonstrated the potential of lipid bodies as hydrophobic storage organelle for sesquiterpenes molecules such as α-bisabolol (Delatte et al., 2018).

CRISPR and Cas9-associated protein systems are emerging as powerful tools to study gene function and to improve specific traits in plant species. Knocking out biosynthetic genes using CRISPR/Cas9 system has been reported in Salvia miltiorrhiza (tanshinone, diterpenoid) (Li et al., 2017) and Papaver somniferum L. (benzylisoquinoline alkaloids) (Alagoz et al., 2016). Setting up CRISPR/Cas9 systems in diverse plant species will be useful for gene function elucidation studies and will also guide future efforts to improve the yield of phytochemicals of interest in valuable medicinal plants. CRISPR/Cas9 systems also have the potential to shut down competing side branches of metabolic pathways within plants, leading to improved yields of the target compound.

Combinatorial Biosynthesis

Engineering new-to-nature metabolic pathways in plants offers the possibility to expand the natural chemical diversity of plants and create a subset of novel chemical structures with new or improved bioactivities. Combinatorial biosynthesis is an engineering strategy, based on combining biosynthetic genes from different sources in a single host, thereby establishing new enzyme–substrate combinations, that enables the generation of a suite of related compounds including many that are new-to-nature (Arendt et al., 2016). This strategy takes advantage of the hypothesis that enzymes involved in plant natural product biosynthesis have inherent relaxed substrate specificity and are often able to transform non-native substrates. Different studies aiming to explore the potential of this approach in creating novel compounds have been reported (Table 2).

TABLE 2
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Table 2. Examples of novel compounds produced through combinatorial biosynthesis using transient expression in N. benthamiana.

Co-agroinfiltration of N. benthamiana with different expression constructs is an efficient method to test numerous enzyme combinations (Reed and Osbourn, 2018). This approach was used to generate novel β-amyrin derivatives through the expression of combinations of β-amyrin synthase and β-amyrin-oxidizing P450s from various plant species (Reed et al., 2017). In another study, the stereoselective biosynthesis of over 50 diterpene skeletons including natural variants and novel compounds was achieved via transient expression of combinations of class I and II diterpene synthases in N. benthamiana (Andersen-Ranberg et al., 2016). These terpene synthases were isolated from seven different diterpenoids producing plant species.

The generation of costunolide and parthenolide derivatives was achieved by applying a combinatorial metabolic engineering approach expressing different enzymes involved in sesquiterpenoids biosynthesis from Tanacetum parthenium and Artemisia annua (Table 2) (Kashkooli Beyraghdar et al., 2019). In a recent study, diverse cytochrome P450s from Setaria italica, Sorghum bicolor, Lotus japonicus, as well as glucosinolate biosynthetic genes from Brassica rapa, were heterologously expressed in N. benthamiana. This expression generated new-to-nature compounds with potent antifungal activity, called crucifalexins. This report showed that by broadening the set of primary metabolites that can be utilized by the core biosynthetic pathway of brassinin (isothiocyanate), it is possible to synthesize novel molecules with enhanced proprieties (Calgaro-Kozina et al., 2020).

Conclusion

Phytochemicals were foundational to the emergence of organic chemistry and the early development of pharmaceuticals. The structural complexity and bioactivity of phytochemicals will ensure their continued relevance in synthetic chemistry and drug discovery. With the maturity of genetic and biochemical tools for gene discovery, characterization and engineering, we are entering into a new synthetic era of phytochemistry, in which biosynthetic pathways are elucidated and high-value phytochemicals can be produced in synthetic biological chasses.

An integrative approach to gene prioritization, combining genomic transcriptomic and metabolomics, has become an efficient method for the identification of candidate biosynthetic genes. Characterization of genes through in vitro assays, coupled with in vivo validation through RNAi or VIGS, subsequently enables verification of candidate gene function. Once the pathway is uncovered, the heterologous in planta expression of the biosynthetic pathway is made possible, with many emerging engineering strategies available to enhance or diversify the production of the target compounds. These advances open the door to a future where diverse phytochemicals with new bioactivities and unusual structures can be routinely produced and purified from heterologous plant systems, aiding drug discovery efforts and leading to the scalable and sustainable production of valuable phytochemicals.

Author Contributions

KE conceived of the manuscript and wrote the first draft. Both authors prepared figures, edited text, and approved the manuscript for submission.

Funding

This work was supported by a UK Research and Innovation Future Leaders Fellowship to BRL (MR/S01862X/1).

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

References

Alagoz, Y., Gurkok, T., Zhang, B., and Unver, T. (2016). Manipulating the biosynthesis of bioactive compound alkaloids for next-generation metabolic engineering in opium poppy Using CRISPR-Cas 9 genome editing technology. Sci. Rep. 6:30910. doi: 10.1038/srep30910

PubMed Abstract | CrossRef Full Text | Google Scholar

Andersen-Ranberg, J., Kongstad, K. T., Nielsen, M. T., Jensen, N. B., Pateraki, I., Bach, S. S., et al. (2016). Expanding the landscape of diterpene structural diversity through stereochemically controlled combinatorial biosynthesis. Angew. Chemie Int. Ed. 55, 2142–2146. doi: 10.1002/anie.201510650

PubMed Abstract | CrossRef Full Text | Google Scholar

Arendt, P., Pollier, J., Callewaert, N., and Goossens, A. (2016). Synthetic biology for production of natural and new-to-nature terpenoids in photosynthetic organisms. Plant J. 87, 16–37. doi: 10.1111/tpj.13138

PubMed Abstract | CrossRef Full Text | Google Scholar

Bock, R. (2015). Engineering plastid genomes: methods, tools, and applications in basic research and biotechnology. Annu. Rev. Plant Biol. 66, 211–241. doi: 10.1146/annurev-arplant-050213-040212

PubMed Abstract | CrossRef Full Text | Google Scholar

Boehm, C. R., and Bock, R. (2019). Recent advances and current challenges in synthetic biology of the plastid genetic system and metabolism. Plant Physiol. 179, 794–802. doi: 10.1104/pp.18.00767

PubMed Abstract | CrossRef Full Text | Google Scholar

Brückner, K., and Tissier, A. (2013). High-level diterpene production by transient expression in Nicotiana benthamiana. Plant Methods 9:46. doi: 10.1186/1746-4811-9-46

PubMed Abstract | CrossRef Full Text | Google Scholar

Calgaro-Kozina, A., Vuu, K. M., Keasling, J. D., Loqué, D., Sattely, E. S., and Shih, P. M. (2020). Engineering plant synthetic pathways for the biosynthesis of novel antifungals. ACS Cent. Sci. 6, 1394–1400. doi: 10.1021/acscentsci.0c00241

PubMed Abstract | CrossRef Full Text | Google Scholar

Cankar, K., Jongedijk, E., Klompmaker, M., Majdic, T., Mumm, R., Bouwmeester, H., et al. (2015). (+)-Valencene production in Nicotiana benthamiana is increased by down-regulation of competing pathways. Biotechnol. J. 10, 180–189. doi: 10.1002/biot.201400288

PubMed Abstract | CrossRef Full Text | Google Scholar

Caputi, L., Franke, J., Farrow, S. C., Chung, K., Payne, R. M. E., Nguyen, T. D., et al. (2018). Missing enzymes in the biosynthesis of the anticancer drug vinblastine in Madagascar periwinkle. Science 360, 1235–1239. doi: 10.1126/science.aat4100

PubMed Abstract | CrossRef Full Text | Google Scholar

Chen, X., Hagel, J. M., Chang, L., Tucker, J. E., Shiigi, S. A., Yelpaala, Y., et al. (2018). A pathogenesis-related 10 protein catalyzes the final step in thebaine biosynthesis article. Nat. Chem. Biol. 14, 738–743. doi: 10.1038/s41589-018-0059-7

PubMed Abstract | CrossRef Full Text | Google Scholar

Courdavault, V., Besseau, S., Oudin, A., Papon, N., and O'Connor, S. E. (2020). Virus-induced gene silencing: hush genes to make them talk. Trends Plant Sci. 25, 714–715. doi: 10.1016/j.tplants.2020.02.013

PubMed Abstract | CrossRef Full Text | Google Scholar

Czechowski, T., Larson, T. R., Catania, T. M., Harvey, D., Brown, G. D., and Graham, I. A. (2016). Artemisia annua mutant impaired in Artemisinin synthesis demonstrates importance of nonenzymatic conversion in terpenoid metabolism. Proc. Natl. Acad. Sci. U.S.A. 113, 15150–15155. doi: 10.1073/pnas.1611567113

PubMed Abstract | CrossRef Full Text | Google Scholar

Delatte, T. L., Scaiola, G., Molenaar, J., de Sousa Farias, K., Alves Gomes Albertti, L., Busscher, J., et al. (2018). Engineering storage capacity for volatile sesquiterpenes in Nicotiana benthamiana leaves. Plant Biotechnol. J. 16, 1997–2006. doi: 10.1111/pbi.12933

PubMed Abstract | CrossRef Full Text | Google Scholar

Dong, L., Jongedijk, E., Bouwmeester, H., and Van Der Krol, A. (2016). Monoterpene biosynthesis potential of plant subcellular compartments. New Phytol. 209, 679–690. doi: 10.1111/nph.13629

PubMed Abstract | CrossRef Full Text | Google Scholar

Duke, M. V., Paul, R. N., Elsohly, H. N., Sturtz, G., and Duke, S. O. (1994). Localization of artemisinin and artemisitene in foliar tissues of glanded and glandless biotypes of Artemisia annua L. Int. J. Plant Sci. 155, 365–372. doi: 10.1086/297173

CrossRef Full Text | Google Scholar

Facchini, P. J., and De Luca, V. (1995). Phloem-specific expression of tyrosine/dopa decarboxylase genes and the biosynthesis of isoquinoline alkaloids in opium poppy. Plant Cell 7, 1811–1821. doi: 10.2307/3870189

PubMed Abstract | CrossRef Full Text | Google Scholar

Freund, D. M., and Hegeman, A. D. (2017). Recent advances in stable isotope-enabled mass spectrometry-based plant metabolomics. Curr. Opin. Biotechnol. 43, 41–48. doi: 10.1016/j.copbio.2016.08.002

PubMed Abstract | CrossRef Full Text | Google Scholar

Fuentes, P., Zhou, F., Erban, A., Karcher, D., Kopka, J., and Bock, R. (2016). A new synthetic biology approach allows transfer of an entire metabolic pathway from a medicinal plant to a biomass crop. Elife 5:e13664. doi: 10.7554/eLife.13664.020

PubMed Abstract | CrossRef Full Text | Google Scholar

Hasan, M. M., Kim, H. S., Jeon, J. H., Kim, S. H., Moon, B. K., Song, J. Y., et al. (2014). Metabolic engineering of Nicotiana benthamiana for the increased production of taxadiene. Plant Cell Rep. 33, 895–904. doi: 10.1007/s00299-014-1568-9

PubMed Abstract | CrossRef Full Text | Google Scholar

Heinig, U., Gutensohn, M., Dudareva, N., and Aharoni, A. (2013). The challenges of cellular compartmentalization in plant metabolic engineering. Curr. Opin. Biotechnol. 24, 239–246. doi: 10.1016/j.copbio.2012.11.006

PubMed Abstract | CrossRef Full Text | Google Scholar

Hileman, L. C., Drea, S., De Martino, G., Litt, A., and Irish, V. F. (2005). Virus-induced gene silencing is an effective tool for assaying gene function in the basal eudicot species Papaver somniferum (opium poppy). Plant J. 44, 334–341. doi: 10.1111/j.1365-313X.2005.02520.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Hodgson, H., De La Peña, R., Stephenson, M. J., Thimmappa, R., Vincent, J. L., Sattely, E. S., et al. (2019). Identification of key enzymes responsible for protolimonoid biosynthesis in plants: opening the door to azadirachtin production. Proc. Natl. Acad. Sci. U.S.A. 116, 17096–17104. doi: 10.1073/pnas.1906083116

PubMed Abstract | CrossRef Full Text | Google Scholar

Huchelmann, A., Boutry, M., and Hachez, C. (2017). Plant glandular trichomes: natural cell factories of high biotechnological interest. Plant Physiol. 175, 6–22. doi: 10.1104/pp.17.00727

PubMed Abstract | CrossRef Full Text | Google Scholar

Jensen, P. E., and Scharff, L. B. (2019). Engineering of plastids to optimize the production of high-value metabolites and proteins. Curr. Opin. Biotechnol. 59, 8–15. doi: 10.1016/j.copbio.2019.01.009

CrossRef Full Text | Google Scholar

Kashkooli Beyraghdar, A., van der Krol, A. R., Rabe, P., Dickschat, J. S., and Bouwmeester, H. (2019). Substrate promiscuity of enzymes from the sesquiterpene biosynthetic pathways from Artemisia annua and Tanacetum parthenium allows for novel combinatorial sesquiterpene production. Metab. Eng. 54, 12–23. doi: 10.1016/j.ymben.2019.01.007

CrossRef Full Text | Google Scholar

Kim, C. Y., Mitchell, A. J., Glinkerman, C. M., Li, F. S., Pluskal, T., and Weng, J. K. (2020). The chloroalkaloid (–)-acutumine is biosynthesized via a Fe(II)- and 2-oxoglutarate-dependent halogenase in Menispermaceae plants. Nat. Commun. 11:1867. doi: 10.1038/s41467-020-15777-w

PubMed Abstract | CrossRef Full Text | Google Scholar

Lechner, A., Brunk, E., and Keasling, J. D. (2016). The need for integrated approaches in metabolic engineering. Cold Spring Harb. Perspect. Biol. 8:a023903. doi: 10.1101/cshperspect.a023903

PubMed Abstract | CrossRef Full Text | Google Scholar

Lee, E. J., and Facchini, P. J. (2011). Tyrosine aminotransferase contributes to benzylisoquinoline alkaloid biosynthesis in opium poppy. Plant Physiol. 157, 1067–1078. doi: 10.1104/pp.111.185512

PubMed Abstract | CrossRef Full Text | Google Scholar

Lee, E. K., Jin, Y. W., Park, J. H., Yoo, Y. M., Hong, S. M., Amir, R., et al. (2010). Cultured cambial meristematic cells as a source of plant natural products. Nat. Biotechnol. 28, 1213–1217. doi: 10.1038/nbt.1693

PubMed Abstract | CrossRef Full Text | Google Scholar

Levsh, O., Pluskal, T., Carballo, V., Mitchell, A. J., and Weng, J. K. (2019). Independent evolution of rosmarinic acid biosynthesis in two sister families under the Lamiids clade of flowering plants. J. Biol. Chem. 294, 15193–15205. doi: 10.1074/jbc.RA119.010454

PubMed Abstract | CrossRef Full Text | Google Scholar

Li, B., Cui, G., Shen, G., Zhan, Z., Huang, L., Chen, J., et al. (2017). Targeted mutagenesis in the medicinal plant Salvia miltiorrhiza. Sci. Rep. 7:43320. doi: 10.1038/srep43320

PubMed Abstract | CrossRef Full Text | Google Scholar

Li, S., Li, Y., and Smolke, C. D. (2018). Strategies for microbial synthesis of high-value phytochemicals. Nat. Chem. 10, 395–404. doi: 10.1038/s41557-018-0013-z

PubMed Abstract | CrossRef Full Text | Google Scholar

Lichman, B. R. (2020). The scaffold-forming steps of plant alkaloid biosynthesis. Nat. Prod. Rep. doi: 10.1039/D0NP00031K

PubMed Abstract | CrossRef Full Text | Google Scholar

Lichman, B. R., Godden, G. T., Hamilton, J. P., Palmer, L., Kamileen, M. O., Zhao, D., et al. (2020). The evolutionary origins of the cat attractant nepetalactone in catnip. Sci. Adv. 6:eaba0721. doi: 10.1126/sciadv.aba0721

PubMed Abstract | CrossRef Full Text | Google Scholar

Lichman, B. R., Kamileen, M. O., Titchiner, G. R., Stevenson, C. E. M., Lawson, D. M., and Connor, S. E. O. (2019). Uncoupled activation and cyclization in catmint reductive terpenoid biosynthesis. Nat. Chem. Biol. 15, 71–79. doi: 10.1038/s41589-018-0185-2

PubMed Abstract | CrossRef Full Text | Google Scholar

Liu, Z., Suarez Duran, H. G., Harnvanichvech, Y., Stephenson, M. J., Schranz, M. E., Nelson, D., et al. (2020). Drivers of metabolic diversification: how dynamic genomic neighbourhoods generate new biosynthetic pathways in the Brassicaceae. New Phytol. 227, 1109–1123. doi: 10.1111/nph.16338

PubMed Abstract | CrossRef Full Text | Google Scholar

Luo, X., Reiter, M. A., d'Espaux, L., Wong, J., Denby, C. M., Lechner, A., et al. (2019). Complete biosynthesis of cannabinoids and their unnatural analogues in yeast. Nature 567, 123–126. doi: 10.1038/s41586-019-0978-9

PubMed Abstract | CrossRef Full Text | Google Scholar

Mitchell, A. J., and Weng, J. K. (2019). Unleashing the synthetic power of plant oxygenases: from mechanism to application. Plant Physiol. 179, 813–829. doi: 10.1104/pp.18.01223

PubMed Abstract | CrossRef Full Text | Google Scholar

Moglia, A., Acquadro, A., Eljounaidi, K., Milani, A. M., Cagliero, C., Rubiolo, P., et al. (2016). Genome-wide identification of BAHD acyltransferases and in vivo characterization of HQT-like enzymes involved in caffeoylquinic acid synthesis in globe artichoke. Front. Plant Sci. 7:1424. doi: 10.3389/fpls.2016.01424

PubMed Abstract | CrossRef Full Text | Google Scholar

Moses, T., Mehrshahi, P., Smith, A. G., and Goossens, A. (2017). Synthetic biology approaches for the production of plant metabolites in unicellular organisms. J. Exp. Bot. 68, 4057–4074. doi: 10.1093/jxb/erx119

PubMed Abstract | CrossRef Full Text | Google Scholar

Moses, T., Pollier, J., Almagro, L., Buyst, D., Van Montagu, M., Pedreño, M. A., et al. (2014). Combinatorial biosynthesis of sapogenins and saponins in Saccharomyces cerevisiae using a C-16α hydroxylase from Bupleurum falcatum. Proc. Natl. Acad. Sci. U.S.A. 111, 1634–1639. doi: 10.1073/pnas.1323369111

PubMed Abstract | CrossRef Full Text | Google Scholar

Munakata, R., Takemura, T., Tatsumi, K., Moriyoshi, E., Yanagihara, K., Sugiyama, A., et al. (2019). Isolation of Artemisia capillaris membrane-bound di-prenyltransferase for phenylpropanoids and redesign of artepillin C in yeast. Commun. Biol. 2:384. doi: 10.1038/s42003-019-0630-0

PubMed Abstract | CrossRef Full Text | Google Scholar

Nett, R. S., Lau, W., and Sattely, E. S. (2020). Discovery and engineering of colchicine alkaloid biosynthesis. Nature 584, 148–153. doi: 10.1038/s41586-020-2546-8

CrossRef Full Text | Google Scholar

Nicolaou, K. C., and Rigol, S. (2020). Perspectives from nearly five decades of total synthesis of natural products and their analogues for biology and medicine. Nat. Prod. Rep. doi: 10.1039/D0NP00003E

PubMed Abstract | CrossRef Full Text | Google Scholar

Nützmann, H.-W., Scazzocchio, C., and Osbourn, A. (2018). Metabolic gene clusters in eukaryotes. Annu. Rev. Genet. 52, 159–183. doi: 10.1146/annurev-genet-120417-031237

PubMed Abstract | CrossRef Full Text | Google Scholar

Nützmann, H. W., Huang, A., and Osbourn, A. (2016). Plant metabolic clusters – from genetics to genomics. New Phytol. 211, 771–789. doi: 10.1111/nph.13981

PubMed Abstract | CrossRef Full Text | Google Scholar

Pluskal, T., Torrens-Spence, M. P., Fallon, T. R., De Abreu, A., Shi, C. H., and Weng, J. K. (2019). The biosynthetic origin of psychoactive kavalactones in kava. Nat. Plants 5, 867–878. doi: 10.1038/s41477-019-0474-0

PubMed Abstract | CrossRef Full Text | Google Scholar

Qu, Y., Safonova, O., and De Luca, V. (2019). Completion of the canonical pathway for assembly of anticancer drugs vincristine/vinblastine in Catharanthus roseus. Plant J. 97, 257–266. doi: 10.1111/tpj.14111

PubMed Abstract | CrossRef Full Text | Google Scholar

Reed, J., and Osbourn, A. (2018). Engineering terpenoid production through transient expression in Nicotiana benthamiana. Plant Cell Rep. 37, 1431–1441. doi: 10.1007/s00299-018-2296-3

PubMed Abstract | CrossRef Full Text | Google Scholar

Reed, J., Stephenson, M. J., Miettinen, K., Brouwer, B., Leveau, A., Brett, P., et al. (2017). A translational synthetic biology platform for rapid access to gram-scale quantities of novel drug-like molecules. Metab. Eng. 42, 185–193. doi: 10.1016/j.ymben.2017.06.012

PubMed Abstract | CrossRef Full Text | Google Scholar

Rontein, D., Onillon, S., Herbette, G., Lesot, A., Werck-Reichhart, D., Sallaud, C., et al. (2008). CYP725A4 from yew catalyzes complex structural rearrangement of taxa-4(5),11(12)-diene into the cyclic ether 5(12)-oxa-3(11)-cyclotaxane. J. Biol. Chem. 283, 6067–6075. doi: 10.1074/jbc.M708950200

PubMed Abstract | CrossRef Full Text | Google Scholar

Roselli, S., Olry, A., Vautrin, S., Coriton, O., Ritchie, D., Galati, G., et al. (2017). A bacterial artificial chromosome (BAC) genomic approach reveals partial clustering of the furanocoumarin pathway genes in parsnip. Plant J. 89, 1119–1132. doi: 10.1111/tpj.13450

PubMed Abstract | CrossRef Full Text | Google Scholar

Sadre, R., Kuo, P., Chen, J., Yang, Y., Banerjee, A., Benning, C., et al. (2019). Cytosolic lipid droplets as engineered organelles for production and accumulation of terpenoid biomaterials in leaves. Nat. Commun. 10:853. doi: 10.1038/s41467-019-08515-4

PubMed Abstract | CrossRef Full Text | Google Scholar

Sadre, R., Magallanes-Lundback, M., Pradhan, S., Salim, V., Mesberg, A., Jones, A. D., et al. (2016). Metabolite diversity in alkaloid biosynthesis: a multilane (diastereomer) highway for camptothecin synthesis in camptotheca acuminata. Plant Cell 28, 1926–1944. doi: 10.1105/tpc.16.00193

PubMed Abstract | CrossRef Full Text | Google Scholar

Schultz, B. J., Kim, S. Y., Lau, W., and Sattely, E. S. (2019). Total biosynthesis for milligram-scale production of etoposide intermediates in a plant chassis. J. Am. Chem. Soc. 141, 19231–19235. doi: 10.1021/jacs.9b10717

PubMed Abstract | CrossRef Full Text | Google Scholar

Shen, Q., Zhang, L., Liao, Z., Wang, S., Yan, T., Shi, P., et al. (2018). The genome of Artemisia annua provides insight into the evolution of asteraceae family and artemisinin biosynthesis. Mol. Plant 11, 776–788. doi: 10.1016/j.molp.2018.03.015

PubMed Abstract | CrossRef Full Text | Google Scholar

Tissier, A., Sallaud, C., and Rontein, D. (2012). “Tobacco trichomes as a platform for terpenoid biosynthesis engineering,” in Isoprenoid Synthesis in Plants and Microorganisms, eds. T. Bach and M. Rohmer (New York, NY: Springer). p. 271–283. doi: 10.1007/978-1-4614-4063-5_18

CrossRef Full Text | Google Scholar

Torrens-Spence, M. P., Pluskal, T., Li, F. S., Carballo, V., and Weng, J. K. (2018). Complete pathway elucidation and heterologous reconstitution of rhodiola salidroside biosynthesis. Mol. Plant 11, 205–217. doi: 10.1016/j.molp.2017.12.007

PubMed Abstract | CrossRef Full Text | Google Scholar

Townsend, T., Segura, V., Chigeza, G., Penfield, T., Rae, A., Harvey, D., et al. (2013). The use of combining ability analysis to identify elite parents for Artemisia annua F1 hybrid production. PLoS ONE 8:e61989. doi: 10.1371/journal.pone.0061989

PubMed Abstract | CrossRef Full Text | Google Scholar

Tu, L., Su, P., Zhang, Z., Gao, L., Wang, J., Hu, T., et al. (2020). Genome of Tripterygium wilfordii and identification of cytochrome P450 involved in triptolide biosynthesis. Nat. Commun. 11:971. doi: 10.1038/s41467-020-14776-1

CrossRef Full Text | Google Scholar

Yoder, R. A., and Johnston, J. N. (2005). A case study in biomimetic total synthesis: polyolefin carbocyclizations to terpenes and steroids. Chem. Rev. 105, 4730–4756. doi: 10.1021/cr040623l

PubMed Abstract | CrossRef Full Text | Google Scholar

Keywords: phytochemicals, gene discovery, biosynthetic pathway, bioactive chemicals, synthetic biology, metabolic engineering, natural products, biosynthesis

Citation: Eljounaidi K and Lichman BR (2020) Nature's Chemists: The Discovery and Engineering of Phytochemical Biosynthesis. Front. Chem. 8:596479. doi: 10.3389/fchem.2020.596479

Received: 19 August 2020; Accepted: 09 October 2020;
Published: 09 November 2020.

Edited by:

Chun Xu, The University of Queensland, Australia

Reviewed by:

Anne Osbourn, John Innes Centre, United Kingdom
Paripok Phitsuwan, King Mongkut's University of Technology Thonburi, Thailand

Copyright © 2020 Eljounaidi and Lichman. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Benjamin R. Lichman, benjamin.lichman@york.ac.uk