Uranium Distribution and Incorporation Mechanism in Deep-Sea Corals: Implications for Seawater [CO₃^2–] Proxies

Abstract

A conservative element in seawater, uranium is readily incorporated into the aragonitic skeletons of scleractinian corals, making them an important paleoclimate archive that can be absolutely dated with U-Th techniques. In addition, uranium concentrations (U/Ca ratios) in corals have been suggested to be influenced by the temperature and/or carbonate ion concentration of the ambient seawater based on empirical calibrations. Microsampling techniques have revealed strong heterogeneities in U/Ca within individual specimens in both surface and deep-sea corals, suggesting a biological control on the U incorporation into the skeletons. Here we further explore the mechanism of uranium incorporation in coral skeletons with the deep-sea species Desmophyllum dianthus, an ideal test organism for the biomineralization processes due to its relatively constant growth environment. We find a negative correlation between bulk coral U/Ca and temperature as well as ambient pH and [CO₃^2–] that is consistent with previous studies. By sampling the growth bands of individual corals, we also find a twofold change in U/Ca within individual corals that is strongly correlated with the δ¹⁸O, δ¹³C, and other Me/Ca ratios of the bands. A similar correlation between U/Ca and stable isotopes as well as other Me/Ca ratios are observed in bulk deep-sea coral samples. With a numerical coral calcification model, we interpret the U/Ca-stable isotope correlation as a result of changes in uranium speciation in response to internal pH elevations in the extracellular calcifying fluid (ECF) of the corals, and suggest that the Ca₂UO₂(CO₃)₃(aq) complex, the dominant U species in seawater, may be the major species incorporated into the coral skeleton. Therefore, the correlation between U/Ca and ambient [CO₃^2–] is likely a result of the response of the biomineralization process, especially the magnitude of internal pH elevation, to the growth environment of the corals. Our data suggest overall lower alkalinity pump rates in corals from low saturation seawater compared to those from high saturation seawater, and possible increases in Ca²⁺ supply from active pumping relative to seawater transport in response to the environmental stress of low saturation.

Introduction

The minor and trace element contents (Me/Ca ratios) of biogenic carbonates have been used for paleoclimate reconstructions for decades. Among them, uranium is of interest due to its relatively high concentrations allowing it to be used as a dating tool as well as a geochemical proxy, especially in biogenic aragonites (Broecker, 1963; Edwards et al., 1987; Min et al., 1995; Shen and Dunbar, 1995; Cheng et al., 2000; Cutler et al., 2003; Russell et al., 2004; Robinson et al., 2006; Anagnostou et al., 2011; Keul et al., 2013; Raddatz et al., 2014; Chen T. et al., 2016; DeCarlo et al., 2016). Uranium is soluble in oxic seawater as uranyl-carbonate complexes and is readily incorporated into the aragonite lattice (Langmuir, 1978; Djogić et al., 1986; Reeder et al., 2000, 2001). However, the exact mechanism of uranium incorporation into carbonate minerals is still under debate (Reeder et al., 2000, 2001; Anagnostou et al., 2011; DeCarlo et al., 2015; Chen X. et al., 2016; Chen, 2020), complicating its use as a paleoclimate proxy. The uranium incorporation mechanism is further complicated by more recent discoveries that the dominant uranium species in seawater are actually Ca-UO₂-CO₃ and Mg-UO₂-CO₃ complexes due to their high stability constants in a medium of high ionic strength (Dong and Brooks, 2006; Endrizzi and Rao, 2014; Chen X. et al., 2016).

Early work on surface corals found seasonal variations in U/Ca that track sea surface temperature (Min et al., 1995; Shen and Dunbar, 1995). Through thermodynamic calculations of a simple exchange reaction (UO₂²⁺ directly substituting Ca²⁺ in aragonite), it was found that the negative correlation between coral U/Ca and temperature is mainly a result of the temperature dependence of the carbonate system constants and its resulting influence on the speciation of uranium, rather than the temperature dependence of uranium partitioning into the mineral (Min et al., 1995). As a result, the incorporation of uranium into coral skeletons can also be influenced by changes in the carbonate chemistry of the ambient seawater (Min et al., 1995; Shen and Dunbar, 1995). This has inspired efforts in developing U/Ca in biogenic carbonates as a proxy for seawater carbonate ion concentrations ([CO₃^2–]), especially in the deep ocean where temperature changes are more limited. Empirical calibrations show that U/Ca in both corals and foraminifera are negatively correlated with seawater [CO₃^2–] or pH (Russell et al., 2004; Anagnostou et al., 2011; Inoue et al., 2011; Raitzsch et al., 2011; Keul et al., 2013; Raddatz et al., 2014; Allen et al., 2016). A negative U/Ca-[CO₃^2–] correlation was also observed in inorganic aragonite precipitation experiments in seawater, without significant influence from temperature or pH, although the apparent sensitivity of U/Ca to [CO₃^2–] is different between laboratory-grown aragonite and coral skeletons (DeCarlo et al., 2015).

Different ideas have been proposed for this negative correlation regarding both the uranium species that gets incorporated into the mineral and the exact incorporation mechanism (Reeder et al., 2000; Anagnostou et al., 2011; DeCarlo et al., 2015; Chen X. et al., 2016; Chen, 2020). In solutions of low ionic strength, UO₂(CO₃)₃^4– is the dominant species above pH = 8 and increases in abundance with higher pH (Langmuir, 1978; Djogić et al., 1986). Spectroscopic evidence suggests that uranium in aragonite has the same bonding structure as aqueous UO₂(CO₃)₃^4– (Reeder et al., 2000). Therefore, the negative correlation between carbonate U/Ca and seawater [CO₃^2–] may be a result of a reduced adsorption efficiency for UO₂(CO₃)₃^4– at high [CO₃^2–] (Barnett et al., 2000; Anagnostou et al., 2011). Such a model, however, does not account for the more complicated uranium speciation in seawater more recently discovered (Dong and Brooks, 2006; Endrizzi and Rao, 2014; Chen X. et al., 2016). The biomineralization process of the calcifying organisms, especially the internal pH elevation in the calcifying fluid of the organisms (Al-Horani et al., 2003; Zoccola et al., 2004; de Nooijer et al., 2009; Venn et al., 2011; Toyofuku et al., 2017), could bring additional complexity to the U/Ca-[CO₃^2–] relation observed in biogenic carbonates without good constraints on the actual carbonate chemistry of the calcifying fluid.

Here we further study the incorporation mechanism of uranium in biogenic aragonite with deep-sea corals as a test organism. Deep-sea corals are well-suited to study the impact of the biomineralization process on geochemical tracers (i.e., “vital effects”), due to their relatively constant growth environment and the large tracer gradient observed in individual specimens (Adkins et al., 2003; Rollion-Bard et al., 2003, 2010; Robinson et al., 2006, 2014; Gagnon et al., 2007). Strong tracer correlations have been found in the skeletons of individual deep-sea corals that can be attributed to a common biomineralization process (Adkins et al., 2003; Gagnon et al., 2007; Chen S. et al., 2018). In particular, the strong correlation between carbon and oxygen isotopes (δ¹⁸O and δ¹³C) of deep-sea coral skeletons has been quantitatively explained with a process-based coral calcification model, which provides the potential to realistically constrain the carbonate chemistry of the extracellular calcifying fluid (ECF) of the corals (Chen S. et al., 2018). As a result, we can explore the fundamental mechanisms of vital effects in the incorporation of minor and trace elements in corals by making coupled Me/Ca and stable isotope measurements. With a suite of modern Desmophyllum dianthus specimens, we show a consistent relation between U/Ca and the stable isotopes (δ¹⁸O and δ¹³C) as well as other Me/Ca ratios of the skeletons in bulk samples and within individual corals. We then discuss the incorporation mechanism of uranium in corals and its implications for U/Ca as a paleo-[CO₃^2–] proxy.

Materials and Methods

Sample Preparation

The D. dianthus specimens used in this study were either provided by the Smithsonian Museum of Natural History or collected from Seamounts south of Tasmania with the deep submergence vehicle Jason during cruise TN-228 in 2008–2009 on the R/V Thompson. Most corals were collected with tissue remains on them and are therefore considered modern. They span a wide range of geographic locations and environmental conditions (Table 1). The environmental conditions of the corals are estimated from nearby stations in the GLODAP v2 database (Key et al., 2015; Olsen et al., 2016) with Ocean Data View (Table 1). The carbonate chemical speciation of the ambient seawater is calculated with alkalinity and dissolved inorganic carbon (DIC) in the GLODAP v2 database using CO2SYS, with carbonate chemistry constants from Millero (2010) and in-situ estimates of silicate and phosphate concentrations for each coral.

For bulk coral samples, a small piece containing both the septa and theca was removed from the coral calice. After physically abrading the outer surface, the coral piece was rinsed in ethanol and DI water, dried with compressed air, and crushed into fine powder with mortar and pestle, before chemical processing and geochemical measurements. A few to tens of milligrams of powder were collected for each coral. Different aliquots of the same powder sample were used for stable isotope and Me/Ca measurements.

To study the tracer variability within individual corals, we also sampled growth features in cross sections of the coral skeletons with a Merchantek micromill system, with a method similar to Adkins et al. (2003) and Gagnon et al. (2007). To generate coupled Me/Ca and stable isotope measurements on the same samples, we micromilled growth bands that are typically 100 μm wide, 200 μm deep, and 1–3 mm long. This allowed us to collect 30–100 μg of powder for stable isotope measurements and 50–200 μg of powder for Me/Ca measurements of the same growth band, and directly compare the distribution patterns in different tracers.

Geochemical Analysis

The stable isotopes were measured at Scripps Institution of Oceanography using a Thermo MAT 253 mass spectrometer equipped with a Kiev IV carbonate preparation device. The samples were measured against an in-house reference gas with an isotopic composition originally calibrated to the Vienna Pee Dee Belemnite (VPDB) scale through the NBS-17 gas standard. The carbonate samples were further calibrated to the VPDB scale with the NBS-19 carbonate standard. The long-term reproducibility (1σ) for carbonate measurements in the mass range employed here is 0.03‰ for δ¹³C and 0.08‰ for δ¹⁸O.

The Me/Ca ratios of the samples were measured in two different labs at the University of St. Andrews and the California Institute of Technology. Measurements of the micromilled samples were split between the two labs, while all the bulk samples were measured at Caltech. With a cleaning test at University of St. Andrews, we found minimal impact of a two-step chemical cleaning (0.1 M NH₄OH+1% H₂O₂ and 0.0005 M HNO₃) on measured U/Ca compared to uncleaned powder from the same coral sample. As a result, the samples were directly dissolved in 5% HNO₃ before Me/Ca analyses. Dilutions of the primary solutions (1:100 at St. Andrews, 1:40 at Caltech) were made to check for [Ca²⁺] with ⁴⁸Ca intensity on the mass spectrometer, after which 1 mM [Ca²⁺] secondary solutions were made for the Me/Ca measurements. The measurements were performed on an Agilent 7500a ICP-MS at St. Andrews, and an Agilent 7500cx ICP-MS at Caltech. Multiple elements were collected during the analyses, including Li, B, Mg, Ca, Mn, Sr, Cd, Ba, and U. The same method was used for the bulk and micromilled samples. This paper specifically focuses on uranium due to its complex speciation as compared to other metal cations. Spatial distributions of other elements in deep-sea coral skeletons are also presented to make cross correlations with uranium, but the detailed mechanisms of these elements will be discussed elsewhere.

The U/Ca ratios of the D. dianthus samples were calibrated with well characterized in-house multi-element standards with compositions similar to biogenic carbonates. Coral standard Jcp-1 was measured with the samples to check for the accuracy of the measurements. We obtained U/Ca ratios of 1.192 ± 0.012 μmol/mol (N = 3) at St. Andrews and 1.188 ± 0.005 μmol/mol (N = 15) at Caltech, both within uncertainty of the interlab calibration value of 1.192 ± 0.045 (1σ) μmol/mol (Hathorne et al., 2013), suggesting our U/Ca measurements are accurately calibrated with sub-percent precision.

Coral Calcification Modeling

The goal of this study is to couple stable isotopes and U/Ca to study the uranium incorporation mechanism. The coral calcification model developed by Chen S. et al. (2018) for stable isotopes is used to constrain the carbonate chemistry of the ECF of the corals together with the stable isotope measurements. The model can quantitatively explain all the δ¹⁸O and δ¹³C features in deep-sea corals in the framework of internal pH elevation modulated by the enzyme carbonic anhydrase (Chen S. et al., 2018). As a result, we expect the model to realistically simulate the carbonate chemistry of the ECF of the corals corresponding to a given stable isotope measurement.

The incorporation of minor and trace elements (with Mg²⁺ as an example) into the coral skeleton can be modeled with the following equations:

Both equations include a seawater flushing term with a timescale of τ_sw. The time scale of seawater turnover should be the same for all geochemical tracers, and is constrained to be a few minutes for D. dianthus based on the stable isotopes (Chen S. et al., 2018). For Ca²⁺, there is an additional source from the alkalinity pump (F_Alk) by membrane bond Ca-ATPase. The f_Ca term in the alkalinity pump flux represents the fraction of the total alkalinity flux from Ca²⁺ pumping, and accounts for the fact that other forms of alkalinity pumps have been described in corals (Jokiel, 2013; Barott et al., 2015; Barron et al., 2018). Ca²⁺ is removed from the ECF by aragonite precipitation (F_{CaCO_3}), with z being the thickness of the ECF (10 μm) to convert fluxes into concentration changes. For Mg²⁺, the only source is from seawater flushing, and the sink term is co-precipitation with aragonite with a distribution coefficient of D_Mg [D_Mg = (Mg/Ca)_coral /(Mg/Ca)_ECF].

As a trace element, the speciation of uranium is dominated by the major ions and the carbonate system. With the carbonate chemistry of the ECF constrained by the stable isotope measurements and the model, we can calculate the corresponding uranium speciation to simulate its incorporation process, and therefore study its incorporation mechanism into coral skeletons. To calculate uranium speciation, we use stability constants determined for high ionic strength conditions from Guillaumont et al. (2003) and Endrizzi and Rao (2014). As with Ca and Mg, the incorporation of uranium into the coral skeleton can be modeled with the equation:

Here we introduce the term χ_U as the fraction of the uranium species that gets incorporated into the coral skeleton in total uranium, and test different incorporation mechanisms with the uranium speciation calculations. Following the definition of χ_U, the U/Ca of the coral aragonite can be calculated as:

We note that there are different definitions of D_U in previous inorganic precipitation experiments regarding whether the denominator should be [Ca²⁺] or [CO₃^2–] in the U/Ca ratio (Meece and Benninger, 1993; Gabitov et al., 2008; Raddatz et al., 2014; DeCarlo et al., 2015). We choose [Ca²⁺] because it is more commonly used in the literature, and uranium most likely substitutes for calcium in the aragonite lattice regardless of the species that is preferentially incorporated (Min et al., 1995; Reeder et al., 2000). Together with the stable isotopes, major ion concentrations and the corresponding carbonate chemistry, we can calculate the steady state [U]_ECF and U/Ca of the coral skeleton.

Results

Empirical Tracer Calibration of Bulk Samples

Figure 1 shows plots of bulk D. dianthus U/Ca with temperature and carbonate chemistry of the ambient seawater. We see that coral U/Ca is negatively correlated with temperature, seawater [CO₃^2–] and pH, consistent with previous studies (Min et al., 1995; Shen and Dunbar, 1995; Anagnostou et al., 2011; Inoue et al., 2011; Raddatz et al., 2014). The correlation with [CO₃^2–] (R = –0.70, p < 0.001) is stronger than with pH (R = –0.66, p = 0.002) and temperature (R = –0.55, p = 0.015), although all three are statistically significant. However, we observe a wide range of scatter in these correlations that is similar to the data collected by Anagnostou et al. (2011) with laser ablation ICP-MS, despite using different analytical methods and calibration standards that could cause measurement offsets in the same corals (Table 1). In our sample set, two corals from the Mediterranean Sea seem to deviate from the U/Ca-[CO₃^2–] trend defined by other corals, suggesting possible changes in their biomineralization process in a special environmental setting (Figure 1B). When these two corals are excluded, the U/Ca-[CO₃^2–] relation we obtained is also statistically indistinguishable from the one found by Anagnostou et al. (2011). We also find that replacing [CO₃^2–] with Δ[CO₃^2–] ([CO₃^2–]_in–situ–[CO₃^2–]_saturation) slightly improves the U/Ca calibration (R = –0.75, p < 0.001), although the scatter is similar with [CO₃^2–] and Δ[CO₃^2–] (Figures 1C,D). No clear correlations are found for U/Ca with other environmental conditions.

FIGURE 1

Tracer Distribution in Individual Desmophyllum dianthus

Figure 2 shows an example of the distribution of stable isotopes and some Me/Ca ratios from the micromilled samples in coral 47394. As with previous studies, we find a relatively wide range of tracer variability within a single D. dianthus specimen (Adkins et al., 2003; Robinson et al., 2006; Gagnon et al., 2007; Case et al., 2010; Stewart et al., 2016). In a single septum of 47394, we see a 3.5‰ range in δ¹⁸O, an 8‰ range in δ¹³C, a 5% change in Sr/Ca, a 24% change in B/Ca, and almost twofold changes in Mg/Ca and U/Ca (Figure 2). Similar ranges of tracer variability are observed in other corals we sampled. In general, the magnitude of tracer variability we observe in individual corals is smaller than previous studies. For U/Ca in particular, Robinson et al. (2006) observed threefold U/Ca changes in individual D. dianthus with a neutron bombardment and fission track counting method. The narrower range in our data is most likely a result of the micromilling procedure, which sampled relatively large amounts of powder for both stable isotopes and Me/Ca measurements and caused significant spatial averaging, compared to methods used in previous studies. However, the tracer variability we were able to retrieve still covers a sufficiently large gradient that can be used to infer the underlying biomineralization process. We chose this spatial averaging so that measurements of Me/Ca ratios and of stable isotopes could be made on the exact same powder while still preserving the natural banding resolution.

FIGURE 2

As with previous observations, we also see distinct isotope and Me/Ca compositions in the center of calcification (COC) bands in deep-sea corals, including depletions in δ¹⁸O, δ¹³C, B/Ca U/Ca, and enrichment in Mg/Ca (Adkins et al., 2003; Blamart et al., 2007; Gagnon et al., 2007; Case et al., 2010; Stewart et al., 2016). There is no distinct Sr/Ca change in the COC, also consistent with previous work (Gagnon et al., 2007; Stewart et al., 2016). Such tracer correlations can be used to infer whether the distinct composition of COCs can be explained with the same biomineralization mechanism as the fibrous secondary aragonite.

U/Ca Correlations With Other Tracers

The distribution of tracers with growth bands points toward strong tracer correlations in individual D. dianthus skeletons. This paper focuses on uranium, and the correlations between other tracers will be discussed elsewhere. Figure 3 shows U/Ca correlations with Mg/Ca, Sr/Ca, δ¹⁸O, and δ¹³C in micromilled bands of individual D. dianthus. There is generally a negative correlation between Mg/Ca and U/Ca, and positive correlations for U/Ca with Sr/Ca, δ¹⁸O and δ¹³C. The COCs may have different tracer correlations from the secondary aragonite, but it is not obvious from the data we collected, which could be a result of the spatial averaging during the micromilling process. Coral 48744 from the Mediterranean Sea shows limited tracer variability in the area we sampled, but still exhibits similar tracer correlation patterns as other corals. The correlations we observe between Mg/Ca, Sr/Ca, and U/Ca are consistent with previous studies of deep-sea corals as well as surface corals (Sinclair, 2005; Gagnon et al., 2007; Raddatz et al., 2014; DeCarlo et al., 2016; Stewart et al., 2016). By coupling Me/Ca ratios with stable isotopes, we also document for the first time the strong positive correlations between U/Ca, δ¹⁸O, and δ¹³C. Given our interpretation of the stable isotopes in deep-sea corals (Adkins et al., 2003; Chen S. et al., 2018), such correlation suggests a decrease in uranium incorporation into the coral skeletons as the alkalinity pump rate is increased in the ECF.

FIGURE 3

In addition to the micromilled growth bands in individual corals, we also observe strong tracer correlations in our bulk coral samples (Figure 4) that are similar to the micromilled samples (Figure 3). For U/Ca-Mg/Ca and U/Ca-δ¹⁸O, there seems to be a single correlation trend in the bulk samples that is consistent with the micromilled samples in individual corals (Figures 4A,C). However, corals from supersaturated and undersaturated seawater show different ranges in tracer values. Corals from undersaturated seawater generally have low Mg/Ca, high U/Ca and enriched δ¹⁸O values and vice versa (Figures 4A,C). For U/Ca-Sr/Ca, no clear correlation is observed when all the corals are considered. However, corals from undersaturated seawater exhibit positive U/Ca-Sr/Ca correlations that are similar to the micromill data, while corals from supersaturated seawater do not show a clear U/Ca-Sr/Ca correlation (Figure 4B). For U/Ca and δ¹³C, two separate correlation trends are observed in the bulk data (Figure 4D). Corals from warm (T > 5°C) supersaturated seawater define a U/Ca-δ¹³C relation with a shallower slope, while the other corals define a steeper U/Ca-δ¹³C slope. Most of the corals on the steep U/Ca-δ¹³C trend live in undersaturated seawater, with two corals from supersaturated seawater at low temperatures (T < 5°C). The separation in U/Ca-δ¹³C slopes can also be seen in the micromill data, where there is a wider spread in the U/Ca-δ¹³C correlations compared to the U/Ca-δ¹⁸O correlations (Figures 3C,D).

FIGURE 4

Uranium Speciation in Seawater

Figure 5 shows uranium speciation changes with pH in seawater at either a constant alkalinity or a constant DIC with stability constants from Guillaumont et al. (2003) and Endrizzi and Rao (2014). We see that the dominant uranium species is the uncharged Ca₂UO₂(CO₃)₃(aq) (58.2%), followed by MgUO₂(CO₃)₃^2– (19.1%) and CaUO₂(CO₃)₃^2– (16.7%). UO₂(CO₃)₃^4–, the dominant species in freshwater at alkaline pH, only constitutes a minor fraction (6.0%) of total uranium under seawater conditions. All other uranium species have negligible contributions to total uranium. Over much of the pH range covered, there is little change in the relative contributions of the dominant uranium species because we assumed constant [Ca²⁺] and [Mg²⁺] in the calculations.

FIGURE 5

Discussion

Uranium Speciation Changes in the Biomineralization Process

In contrast to seawater with constant [Ca²⁺] and [Mg²⁺], there are more dynamic changes in the carbonate chemistry and [Ca²⁺] in the ECF during the biomineralization process, which can significantly impact the uranium speciation. Figure 6 shows uranium speciation changes in the ECF in response to the internal pH up-regulation by the alkalinity pump at different f_Ca values. Changing f_Ca significantly alters the [Ca²⁺] dynamics in the ECF and has strong impacts on the speciation of uranium. When f_Ca = 1, the Ca²⁺ pump is balanced by aragonite precipitation in the model at different pump rates, maintaining a relatively constant [Ca²⁺] in the ECF and therefore small changes in U speciation, until UO₂(OH)₃^– takes over at high pH. In contrast, when f_Ca = 0, Ca²⁺ is removed from the ECF by aragonite precipitation with supply coming only from seawater. Therefore, [Ca²⁺] decreases in the ECF for this end member scenario as the alkalinity pump and precipitation rate increase, causing a decrease in Ca₂UO₂(CO₃)₃ and an increase in MgUO₂(CO₃)₃^2– at higher pH. We expect such changes in uranium speciation to influence the amount of uranium incorporated into the coral skeleton, depending on the species that is preferentially taken into the aragonite lattice.

FIGURE 6

Uranium Incorporation Mechanism

Given the sensitivity of uranium speciation to [Ca²⁺] and the carbonate chemistry of the solution, the U/Ca proxy is expected to be strongly influenced by the biomineralization process. X-ray absorption experiments in synthetic carbonates showed that uranyl in aragonite has the same bonding configuration as aqueous UO₂(CO₃)₃^4–, suggesting intact incorporation of the whole unit in place of a Ca polyhedron with three CO₃^2– units (Reeder et al., 2000). These precipitation experiments, however, were performed in low ionic strength solutions without significant contributions of the Ca-UO₂-CO₃ and Mg-UO₂-CO₃ complexes to the uranium speciation. Aragonite precipitation experiments in seawater by DeCarlo et al. (2015) revealed a dependence of the uranium distribution coefficient on [CO₃^2–] and suggested uranium speciation changes as the most likely mechanism, although their speciation calculations do not consider the Ca-UO₂-CO₃ and Mg-UO₂-CO₃ complexes, either. Recent work on uranium isotopes in synthetic carbonates grown in NaCl solution found very small to no fractionation between the solution and the mineral (Chen X. et al., 2016). Chen X. et al. (2016) suggested the observed small δ²³⁸U enrichment (0.11‰) in aragonite relative to aqueous uranium could be a result of the preferential incorporation of the charged complexes [CaUO₂(CO₃)₃^2– and MgUO₂(CO₃)₃^2–] that are expected to be enriched in ²³⁸U. However, the ²³⁸U enrichment was observed in only one aragonite experiment, and their later work on biogenic carbonates found little fractionation between multiple coral species and seawater (Chen X. et al., 2018), suggesting either no particular preference for the incorporation of any uranium species or little isotope fractionation between the species. We can further test these ideas with the observed tracer correlations in deep-sea corals.

Among the tracer correlations observed in D. dianthus, the most robust one is between U/Ca and δ¹⁸O in both the micromill and bulk data (Figures 3C, 4C). The positive U/Ca-δ¹⁸O correlations are almost identical for the micromill and bulk data. The sign of the correlation suggests a depletion in uranium incorporation with lower δ¹⁸O, which corresponds to higher alkalinity pump rates and ECF pH. A growth rate effect cannot explain a U/Ca decrease with higher pH, since the distribution coefficient for uranium is expected to increase at higher growth rates in aragonite (Gabitov et al., 2008). Following Equation (3), a decrease in uranium incorporation at higher pH is most likely a result of changes in total uranium concentration and the abundance of the preferentially incorporated uranium species relative to Ca²⁺ in the ECF. We can explore which uranium species is preferentially incorporated by comparing observed tracer correlations with model results.

Figure 7 shows the data-model comparison for U/Ca correlations with other tracers under different uranium incorporation scenarios. We see that in order to get the correct signs of tracer correlations in the data, the distribution coefficient for uranium (D_U) has to be greater than 1, regardless of the uranium species incorporated. With D_U > 1, uranium is preferentially removed from the ECF over Ca, causing a decrease in U/Ca at higher alkalinity pump rates and ECF pH. With such a distillation process, U/Ca in corals have lower values and are more sensitive to [CO₃^2–] than inorganic aragonite (DeCarlo et al., 2015). In terms of the uranium species incorporated into the coral skeleton, we get a better match between the data and the model when we either consider total uranium (all uranium species are incorporated without particular preference) or the dominant species Ca₂UO₂(CO₃)₃, as compared to the other species (Figure 7). Between U_total and Ca₂UO₂(CO₃)₃, we see that the model parameter set with Ca₂UO₂(CO₃)₃ incorporation at f_Ca = 0 fits most of the features in U/Ca correlations with δ¹⁸O, Mg/Ca, and Sr/Ca (Figures 7E,J,O). For U_total, the f_Ca values required to fit the U/Ca-δ¹⁸O and U/Ca-Mg/Ca correlations are different, and the model cannot create the U/Ca-Sr/Ca slope in the majority of the data regardless of the f_Ca value (Figures 7A,F,K). As a result, our tracer data and biomineralization model favors Ca₂UO₂(CO₃)₃ as the uranium species that is preferentially incorporated into the coral skeletons. Ca₂UO₂(CO₃)₃ has several advantages in terms of incorporation into the aragonite lattice. Besides being the dominant aqueous uranium species in seawater, it is also uncharged and has a symmetric structure that is similar to the arrangement of Ca²⁺ and CO₃^2– ions in the aragonite lattice (Wu et al., 2016). This may allow it to substitute for three whole CaCO₃ unit cells without much distortion in the structure of the mineral. This could explain the lack of uranium isotope fractionation between coral skeletons and seawater observed by Chen X. et al. (2018). Stoichiometrically, such a substitution is equivalent to a UO₂²⁺-for-Ca²⁺ swap, which may also explain why the calculations by Min et al. (1995) were able to predict the temperature sensitivity of U/Ca based on a simple UO₂²⁺-for-Ca²⁺ exchange reaction.

FIGURE 7

In addition to diagnosing the uranium species preferentially incorporated, the data-model comparison also suggests that the tracer correlation patterns are best explained by the f_Ca = 0 case. This is most clearly reflected in the U/Ca-Mg/Ca and U/Ca-Sr/Ca correlations, where f_Ca = 0 yields the slopes that best match the data. When f_Ca = 0.5 or 1, the simulated range in Mg/Ca is too small while the range in Sr/Ca is too large for the same amount of U/Ca changes, and is related to the different distribution coefficients for these two elements in aragonite (D_Mg < < 1, D_Sr > 1), which has been previously suggested (Gaetani and Cohen, 2006; Gagnon et al., 2007; Gaetani et al., 2011) and will be detailed in a separate paper. In brief, the observed tracer correlations point toward a case of limited Ca²⁺ pumping (low f_Ca) in the cross-membrane alkalinity flux to the ECF in D. dianthus. Given a 5:1 Ca:DIC ratio (and ∼100:1 Ca²⁺:CO₃^2– ratio) in seawater, it is a better strategy for the corals to elevate DIC and pH in the ECF to facilitate precipitation with alkalinity pumps that are more efficient than Ca-ATPase, instead of spending a lot of energy in elevating [Ca²⁺]_ECF. Quantifying the relative importance of various alkalinity pumps in different marine calcifying organisms is an interesting target for future culture experiments.

Finally, it should be noted that our model is not able to fit all the features in the tracer data. The misfit is mostly seen at the high pH end with depleted δ¹⁸O, high Mg/Ca, and low U/Ca (Figure 7). There is a kink in most model curves in Figure 7 related to drastic carbonate chemistry changes as the pH is elevated above pK_a2 of the carbonate system, which is also reflected in the δ¹⁸O-δ¹³C correlation in the COCs (Adkins et al., 2003; Chen S. et al., 2018). It is possible that the COCs have a different incorporation mechanism for minor and trace elements, as evidenced by their unique elemental compositions and tracer correlations (high Mg/Ca, low B/Ca, and U/Ca, intermediate Sr/Ca, Figure 2). This could complicate the empirical tracer calibrations in bulk coral samples. It is also possible that the exact uranium incorporation mechanism in aragonite is more complicated than our simple model can currently account for.

Implications for U/Ca as a [CO₃^2–] Proxy

With the micromill data and the biomineralization model, we are able to find a scenario that explains most of the tracer data in D. dianthus, with Ca₂UO₂(CO₃)₃ as the incorporated uranium species and limited Ca²⁺ pumping (low f_Ca). This could give us clues to the impact of the biomineralization process on empirical tracer calibrations for U/Ca. The observation that U/Ca is slightly better correlated with Δ[CO₃^2–] than [CO₃^2–] (Figures 1C,D) suggests that the saturation state of seawater plays a role in uranium incorporation. We see from bulk tracer correlations that corals living in high saturation seawater tend to have lower δ¹⁸O, higher Mg/Ca and lower U/Ca compared to their counterparts from low saturation seawater (Figure 4), suggesting the corals have overall higher alkalinity pump rates when the environmental conditions are favorable for calcification. The negative U/Ca-[CO₃^2–] correlation from bulk D. dianthus samples is most likely a result of the response of the strength of the alkalinity pump to the ambient environment. On the other hand, the scatter in the U/Ca-[CO₃^2–] correlation is likely a result of the differences in the magnitude of internal pH elevation in individual corals that was randomly sampled in the bulk powder, given a twofold or more change in U/Ca in individual corals. For example, the two corals from the Mediterranean Sea have higher U/Ca than the U/Ca-[CO₃^2–] trend defined by other corals would suggest (Figure 1C). However, the bulk δ¹⁸O of these two corals are also closer to equilibrium than the other corals from supersaturated seawater, suggesting overall lower alkalinity pump rates. This may be the result of a sampling bias, but can also be related to the corals lowering the energetic cost of alkalinity pumping when the growth environment is favorable for calcification. A similar observation can be made on Lophelia pertusa (recently reclassified as Desmophyllum pertusum by Addamo et al., 2016), another deep-sea coral species. Although U/Ca in L. pertusa was found to be better correlated with pH than with [CO₃^2–] by Raddatz et al. (2014), the Mediterranean samples show a different U/Ca-[CO₃^2–] relation from their North Atlantic counterparts. While pH and [CO₃^2–] are often strongly correlated in seawater, Mediterranean seawater has very different physical and chemical properties from the open ocean and thus a different pH-[CO₃^2–] relation, which is manifested by higher [CO₃^2–] at similar pH values (Flögel et al., 2014) and influences the U/Ca calibrations in both D. dianthus and L. pertusa. Flögel et al. (2014) also suggested that high DIC in the Mediterranean Sea may negatively impact the growth of L. pertusa and limit its colony size, which could also affect D. dianthus if they share a similar calcification mechanism. Overall, the stronger correlations for bulk U/Ca with δ¹⁸O and Mg/Ca compared to ambient [CO₃^2–] indicate dominant control by the biomineralization process on the U/Ca proxy. This also supports the use of U/Ca as a normalizer for the vital effects in the development of better proxies such as the Sr-U thermometer in corals (DeCarlo et al., 2016).

While the strong biological control on U incorporation complicates the application of the U/Ca proxy, the observed correlations between U/Ca and other tracers may provide insights into the response of the coral biomineralization process to the environmental conditions. We see in Figure 7 that increasing the fraction of Ca²⁺ in the alkalinity pump (f_Ca) can cause changes in U/Ca variability relative to other tracers and generate different tracer correlation slopes. Another way to change the sensitivity of U/Ca to the alkalinity pump in our model is to change the seawater turnover timescale (τ_sw). Figure 8 shows the sensitivity of U/Ca to ECF pH and [CO₃^2–] as well as U/Ca correlations with δ¹³C and Mg/Ca at different seawater turnover rates. We see that U/Ca is more sensitive to ECF pH and [CO₃^2–] at low pump rates, causing a concave-down trend in the correlations (Figures 8A,B). The low U/Ca sensitivity to ECF pH and [CO₃^2–] at high pump rates could explain the observation of low U/Ca extending beyond the COCs in D. dianthus (Robinson et al., 2006). U/Ca sensitivity to pH and [CO₃^2–] increases when seawater turnover is slowed down (longer τ_sw), and the corresponding U/Ca-δ¹³C slope is also steeper (Figure 8C). The U/Ca-Mg/Ca correlation trend is not influenced by τ_sw in our simple model, but the range of variability in U/Ca and Mg/Ca also increases with τ_sw (Figure 8D). As seawater turnover is slowed down, the ECF is more like a closed system that increases the distillation of uranium relative to Ca²⁺ and causes larger changes in U/Ca. The change in U/Ca-δ¹³C slope in the bulk D. dianthus data (Figure 4D) could be a result of individual corals adapting to their growth environment by changing their calcification strategies. Corals living in high saturation seawater tend to have a relatively fast seawater turnover and low f_Ca in the alkalinity pump while pumping to relatively high pH in the ECF, all of which tend to reduce U/Ca sensitivity to the biomineralization process. On the contrary, corals living in a low saturation environment would slow down the turnover of the corrosive seawater and have to make up for the Ca²⁺ supply by increasing f_Ca in the alkalinity pump. In addition, the magnitude of pH elevation is likely more limited in corals from low saturation seawater (as shown by the range in stable isotopes), further increasing the sensitivity of U/Ca to the calcification dynamics. Such differences in biomineralization strategies can explain the general trend in the U/Ca proxy calibration (Figure 1), but also adds complexity to its potential paleoceanographic applications. A multi-proxy approach is required to fully disentangle the vital effects from environmental imprints in different geochemical tracers in deep-sea corals and other biogenic carbonates.

FIGURE 8

Conclusion

We have combined stable isotopes, Me/Ca ratios and a numerical model of coral calcification to study uranium incorporation in deep-sea corals. While bulk U/Ca in D. dianthus show statistically significant correlations with temperature and ambient [CO₃^2–], there is significant scatter in the empirical calibrations that is related to the biomineralization process. Given the strong tracer correlations between U/Ca, Mg/Ca, Sr/Ca, δ¹⁸O, and δ¹³C both in the bulk samples and within the growth bands in individual corals, we suggest that the uncharged Ca₂UO₂(CO₃)₃(aq) complex, the dominant uranium species in seawater, is likely the preferentially incorporated uranium species in coral skeletons. Deep-sea corals may also change their biomineralization strategies in response to the growth environment, including overall lower alkalinity pump rates, increases in the fraction of Ca²⁺ pumping as well as slower seawater turnover when the ambient seawater is less saturated. The empirical correlation between U/Ca and seawater [CO₃^2–] is mainly a result of changes in overall alkalinity pump rates, but can be complicated by the exact calcification strategy used by individual corals. We expect a multi-proxy approach that couples stable isotopes and Me/Ca ratios to better help us better understand the mechanisms of vital effects and use these tracers for paleoceanographic reconstructions.

References

• 1

  AddamoA. M.VertinoA.StolarskiJ.García-JiménezR.TavianiM.MachordomA. (2016). Merging scleractinian genera: the overwhelming genetic similarity between solitary Desmophyllum and colonial Lophelia.BMC Evol. Biol.16:108. 10.1186/s12862-016-0654-8

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 2

  AdkinsJ. F.BoyleE. A.CurryW. B.LutringerA. (2003). Stable isotopes in deep-sea corals and a new mechanism for “vital effects.”.Geochim. Cosmochim. Acta671129–1143. 10.1016/S0016-7037(02)01203-6

  • CrossRef
  • Google Scholar

• 3

  Al-HoraniF. A.Al-MoghrabiS. M.de BeerD. (2003). The mechanism of calcification and its relation to photosynthesis and respiration in the scleractinian coral Galaxea fascicularis.Mar. Biol.142419–426. 10.1007/s00227-002-0981-8

  • CrossRef
  • Google Scholar

• 4

  AllenK. A.HönischB.EgginsS. M.HaynesL. L.RosenthalY.YuJ. (2016). Trace element proxies for surface ocean conditions: a synthesis of culture calibrations with planktic foraminifera.Geochim. Cosmochim. Acta193197–221. 10.1016/j.gca.2016.08.015

  • CrossRef
  • Google Scholar

• 5

  AnagnostouE.SherrellR. M.GagnonA.LaVigneM.FieldM. P.McDonoughW. F. (2011). Seawater nutrient and carbonate ion concentrations recorded as P/Ca, Ba/Ca, and U/Ca in the deep-sea coral Desmophyllum dianthus.Geochim. Cosmochim. Acta752529–2543. 10.1016/j.gca.2011.02.019

  • CrossRef
  • Google Scholar

• 6

  BarnettM. O.JardineP. M.BrooksS. C.SelimH. M. (2000). Adsorption and transport of uranium(VI) in subsurface media.Soil Sci. Soc. Am. J.64908–917. 10.2136/sssaj2000.643908x

  • CrossRef
  • Google Scholar

• 7

  BarottK. L.PerezS. O.LinsmayerL. B.TresguerresM. (2015). Differential localization of ion transporters suggests distinct cellular mechanisms for calcification and photosynthesis between two coral species.Am. J. Physiol. Regulat. Integrat. Compar. Physiol.309R235–R246. 10.1152/ajpregu.00052.2015

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 8

  BarronM. E.ThiesA. B.EspinozaJ. A.BarottK. L.HamdounA.TresguerresM. (2018). A vesicular Na+/Ca2+ exchanger in coral calcifying cells.PLoS One13:e0205367. 10.1371/journal.pone.0205367

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 9

  BlamartD.Rollion−BardC.MeibomA.CuifJ.-P.Juillet−LeclercA.DauphinY. (2007). Correlation of boron isotopic composition with ultrastructure in the deep-sea coral Lophelia pertusa: Implications for biomineralization and paleo-pH.Geochem. Geophys. Geosyst.8:12. 10.1029/2007GC001686

  • CrossRef
  • Google Scholar

• 10

  BroeckerW. S. (1963). A preliminary evaluation of uranium series inequilibrium as a tool for absolute age measurement on marine carbonates.J. Geophys. Res.682817–2834. 10.1029/JZ068i009p02817

  • CrossRef
  • Google Scholar

• 11

  CaseD. H.RobinsonL. F.AuroM. E.GagnonA. C. (2010). Environmental and biological controls on Mg and Li in deep-sea scleractinian corals.Earth Planet. Sci. Lett.300215–225. 10.1016/j.epsl.2010.09.029

  • CrossRef
  • Google Scholar

• 12

  ChenS.GagnonA. C.AdkinsJ. F. (2018). Carbonic anhydrase, coral calcification and a new model of stable isotope vital effects.Geochim. Cosmochim. Acta236179–197. 10.1016/j.gca.2018.02.032

  • CrossRef
  • Google Scholar

• 13

  ChenT.RobinsonL. F.BeasleyM. P.ClaxtonL. M.AndersenM. B.GregoireL. J.et al (2016). Ocean mixing and ice-sheet control of seawater 234U/238U during the last deglaciation.Science354626–629. 10.1126/science.aag1015

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 14

  ChenX. (2020). Aqueous uranium speciation on U/Ca in foraminiferal calcite: the importance of minor species—UO₂(CO₃)₂^2–.ACS Earth Space Chem.42050–2060.

  • Google Scholar

• 15

  ChenX.RomanielloS. J.HerrmannA. D.SamankassouE.AnbarA. D. (2018). Biological effects on uranium isotope fractionation (²³⁸U/²³⁵U) in primary biogenic carbonates. Geochim. Cosmochim. Acta.2401–10. 10.1016/j.gca.2018.08.028

  • CrossRef
  • Google Scholar

• 16

  ChenX.RomanielloS. J.HerrmannA. D.WasylenkiL. E.AnbarA. D. (2016). Uranium isotope fractionation during coprecipitation with aragonite and calcite.Geochim. Cosmochim. Acta188189–207. 10.1016/j.gca.2016.05.022

  • CrossRef
  • Google Scholar

• 17

  ChengH.AdkinsJ.EdwardsR. L.BoyleE. A. (2000). U-Th dating of deep-sea corals.Geochim. Cosmochim. Acta642401–2416. 10.1016/S0016-7037(99)00422-6

  • CrossRef
  • Google Scholar

• 18

  CutlerK. B.EdwardsR. L.TaylorF. W.ChengH.AdkinsJ.GallupC. D.et al (2003). Rapid sea-level fall and deep-ocean temperature change since the last interglacial period.Earth Planet. Sci. Lett.206253–271. 10.1016/S0012-821X(02)01107-X

  • CrossRef
  • Google Scholar

• 19

  de NooijerL. J.ToyofukuT.KitazatoH. (2009). Foraminifera promote calcification by elevating their intracellular pH.Proc. Natl. Acad. Sci.10615374–15378. 10.1073/pnas.0904306106

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 20

  DeCarloT. M.GaetaniG. A.CohenA. L.FosterG. L.AlpertA. E.StewartJ. A. (2016). Coral Sr-U thermometry.Paleoceanography31626–638. 10.1002/2015PA002908

  • CrossRef
  • Google Scholar

• 21

  DeCarloT. M.GaetaniG. A.HolcombM.CohenA. L. (2015). Experimental determination of factors controlling U/Ca of aragonite precipitated from seawater: implications for interpreting coral skeleton.Geochim. Cosmochim. Acta162151–165. 10.1016/j.gca.2015.04.016

  • CrossRef
  • Google Scholar

• 22

  DjogićR.SiposL.BranicaM. (1986). Characterization of uranium(VI) in seawater1.Limnol. Oceanogr.311122–1131. 10.4319/lo.1986.31.5.1122

  • CrossRef
  • Google Scholar

• 23

  DongW.BrooksS. C. (2006). Determination of the formation constants of ternary complexes of uranyl and carbonate with alkaline earth metals (Mg2+, Ca2+, Sr2+, and Ba2+) using anion exchange method.Environ. Sci. Technol.404689–4695. 10.1021/es0606327

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 24

  EdwardsL. R.ChenJ. H.WasserburgG. J. (1987). 238U234U230Th232Th systematics and the precise measurement of time over the past 500,000 years.Earth Planet. Sci. Lett.81175–192. 10.1016/0012-821X(87)90154-3

  • CrossRef
  • Google Scholar

• 25

  EndrizziF.RaoL. (2014). Chemical speciation of uranium(VI) in marine environments: complexation of calcium and magnesium ions with [(UO2)(CO3)3](4-) and the effect on the extraction of uranium from seawater.Chem. (Weinheim Bergstr. Germ.)2014499–14506. 10.1002/chem.201403262

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 26

  FlögelS.DulloW.-C. h. r.PfannkucheO.KiriakoulakisK.RüggebergA. (2014). Geochemical and physical constraints for the occurrence of living cold-water corals.Deep Sea Res. Part II Top. Stud. Oceanogr.9919–26. 10.1016/j.dsr2.2013.06.006

  • CrossRef
  • Google Scholar

• 27

  GabitovR. I.GaetaniG. A.WatsonE. B.CohenA. L.EhrlichH. L. (2008). Experimental determination of growth rate effect on U6+ and Mg2+ partitioning between aragonite and fluid at elevated U6+ concentration.Geochim. Cosmochim. Acta724058–4068. 10.1016/j.gca.2008.05.047

  • CrossRef
  • Google Scholar

• 28

  GaetaniG. A.CohenA. L. (2006). Element partitioning during precipitation of aragonite from seawater: a framework for understanding paleoproxies.Geochim. Cosmochim. Acta704617–4634. 10.1016/j.gca.2006.07.008

  • CrossRef
  • Google Scholar

• 29

  GaetaniG. A.CohenA. L.WangZ.CrusiusJ. (2011). Rayleigh-based, multi-element coral thermometry: a biomineralization approach to developing climate proxies.Geochim. Cosmochim. Acta751920–1932. 10.1016/j.gca.2011.01.010

  • CrossRef
  • Google Scholar

• 30

  GagnonA. C.AdkinsJ. F.FernandezD. P.RobinsonL. F. (2007). Sr/Ca and Mg/Ca vital effects correlated with skeletal architecture in a scleractinian deep-sea coral and the role of Rayleigh fractionation.Earth Planet. Sci. Lett.261280–295. 10.1016/j.epsl.2007.07.013

  • CrossRef
  • Google Scholar

• 31

  GuillaumontR.MompeanF. J.Oecd Nuclear Energy Agency(Eds.) (2003). Update on the Chemical Thermodynamics of Uranium, Neptunium, Plutonium, Americium and Technetium.Amsterdam: Elsevier.

  • Google Scholar

• 32

  HathorneE. C.GagnonA.FelisT.AdkinsJ.AsamiR.BoerW.et al (2013). Interlaboratory study for coral Sr/Ca and other element/Ca ratio measurements.Geochem. Geophys. Geosyst.143730–3750. 10.1002/ggge.20230

  • CrossRef
  • Google Scholar

• 33

  InoueM.SuwaR.SuzukiA.SakaiK.KawahataH. (2011). Effects of seawater pH on growth and skeletal U/Ca ratios of Acropora digitifera coral polyps.Geophys. Res. Lett.3812. 10.1029/2011GL047786

  • CrossRef
  • Google Scholar

• 34

  JokielP. L. (2013). Coral reef calcification: carbonate, bicarbonate and proton flux under conditions of increasing ocean acidification.Proc. R. Soc. B Biol. Sci.280:1764. 10.1098/rspb.2013.0031

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 35

  KeulN.LangerG.NooijerL. J.de NehrkeG.ReichartG.-J.BijmaJ. (2013). Incorporation of uranium in benthic foraminiferal calcite reflects seawater carbonate ion concentration.Geochem. Geophys. Geosyst.14102–111. 10.1029/2012GC004330

  • CrossRef
  • Google Scholar

• 36

  KeyR. M.OlsenA.van HeuvenS.LauvsetS. K.VeloA.LinX.et al (2015). Global Ocean Data Analysis Project, Version 2 (GLODAPv2) [Miscellaneous]. ORNL/CDIAC-162, NDP-093.Ridge, TN: Carbon Dioxide Information Analysis Center, Oak Ridge National Laboratory. 10.3334/CDIAC/OTG

  • CrossRef
  • Google Scholar

• 37

  LangmuirD. (1978). Uranium solution-mineral equilibria at low temperatures with applications to sedimentary ore deposits.Geochim. Cosmochim. Acta42(6 Part A)547–569. 10.1016/0016-7037(78)90001-7

  • CrossRef
  • Google Scholar

• 38

  MeeceD. E.BenningerL. K. (1993). The coprecipitation of Pu and other radionuclides with CaCO3.Geochim. Cosmochim. Acta571447–1458. 10.1016/0016-7037(93)90005-H

  • CrossRef
  • Google Scholar

• 39

  MilleroF. J. (2010). Carbonate constants for estuarine waters.Mar. Freshw. Res.61139–142. 10.1071/MF09254

  • CrossRef
  • Google Scholar

• 40

  MinR. G.EdwardsL. R.TaylorF. W.RecyJ.GallupC. D.Warren BeckJ. (1995). Annual cycles of UCa in coral skeletons and UCa thermometry.Geochim. Cosmochim. Acta592025–2042. 10.1016/0016-7037(95)00124-7

  • CrossRef
  • Google Scholar

• 41

  OlsenA.KeyR. M.HeuvenS.van LauvsetS. K.VeloA.LinX.et al (2016). The global ocean data analysis project version 2 (GLODAPv2) – an internally consistent data product for the world ocean.Earth Syst. Sci. Data8297–323. 10.5194/essd-8-297-2016

  • CrossRef
  • Google Scholar

• 42

  RaddatzJ.RüggebergA.FlögelS.HathorneE. C.LiebetrauV.EisenhauerA.et al (2014). The influence of seawater pH on U / Ca ratios in the scleractinian cold-water coral Lophelia pertusa.Biogeosciences111863–1871. 10.5194/bg-11-1863-2014

  • CrossRef
  • Google Scholar

• 43

  RaitzschM.KuhnertH.HathorneE. C.GroeneveldJ.BickertT. (2011). U/Ca in benthic foraminifers: a proxy for the deep-sea carbonate saturation.Geochem. Geophys. Geosyst.12:6. 10.1029/2010GC003344

  • CrossRef
  • Google Scholar

• 44

  ReederR. J.NugentM.LambleG. M.TaitC. D.MorrisD. E. (2000). Uranyl incorporation into calcite and aragonite: XAFS and luminescence studies.Environ. Sci. Technol.34638–644. 10.1021/es990981j

  • CrossRef
  • Google Scholar

• 45

  ReederR. J.NugentM.TaitC. D.MorrisD. E.HealdS. M.BeckK. M.et al (2001). Coprecipitation of uranium(VI) with calcite: XAFS, micro-XAS, and luminescence characterization.Geochim. Cosmochim. Acta653491–3503. 10.1016/S0016-7037(01)00647-0

  • CrossRef
  • Google Scholar

• 46

  RobinsonL. F.AdkinsJ. F.FernandezD. P.BurnettD. S.WangS.-L.GagnonA. C.et al (2006). Primary U distribution in scleractinian corals and its implications for U series dating.Geochem. Geophys. Geosyst.7:5. 10.1029/2005GC001138

  • CrossRef
  • Google Scholar

• 47

  RobinsonL. F.AdkinsJ. F.FrankN.GagnonA. C.ProutyN. G.Brendan RoarkE.et al (2014). The geochemistry of deep-sea coral skeletons: a review of vital effects and applications for palaeoceanography.Deep Sea Res Part II Top. Stud. Oceanogr.99184–198. 10.1016/j.dsr2.2013.06.005

  • CrossRef
  • Google Scholar

• 48

  Rollion-BardC.BlamartD.CuifJ.-P.DauphinY. (2010). In situ measurements of oxygen isotopic composition in deep-sea coral, Lophelia pertusa: re-examination of the current geochemical models of biomineralization.Geochim. Cosmochim. Acta741338–1349. 10.1016/j.gca.2009.11.011

  • CrossRef
  • Google Scholar

• 49

  Rollion-BardC.ChaussidonM.France-LanordC. (2003). PH control on oxygen isotopic composition of symbiotic corals.Earth Planet. Sci. Lett.215275–288. 10.1016/S0012-821X(03)00391-1

  • CrossRef
  • Google Scholar

• 50

  RussellA. D.HönischB.SperoH. J.LeaD. W. (2004). Effects of seawater carbonate ion concentration and temperature on shell U, Mg, and Sr in cultured planktonic foraminifera.Geochim. Cosmochim. Acta684347–4361. 10.1016/j.gca.2004.03.013

  • CrossRef
  • Google Scholar

• 51

  ShenG. T.DunbarR. B. (1995). Environmental controls on uranium in reef corals.Geochim. Cosmochim. Acta592009–2024. 10.1016/0016-7037(95)00123-9

  • CrossRef
  • Google Scholar

• 52

  SinclairD. J. (2005). Correlated trace element “vital effects” in tropical corals: a new geochemical tool for probing biomineralization.Geochim. Cosmochim. Acta693265–3284. 10.1016/j.gca.2005.02.030

  • CrossRef
  • Google Scholar

• 53

  StewartJ. A.AnagnostouE.FosterG. L. (2016). An improved boron isotope pH proxy calibration for the deep-sea coral Desmophyllum dianthus through sub-sampling of fibrous aragonite.Chem. Geol.447148–160. 10.1016/j.chemgeo.2016.10.029

  • CrossRef
  • Google Scholar

• 54

  ToyofukuT.MatsuoM. Y.de NooijerL. J.NagaiY.KawadaS.FujitaK.et al (2017). Proton pumping accompanies calcification in foraminifera.Nat. Commun.8:14145. 10.1038/ncomms14145

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 55

  VennA.TambuttéE.HolcombM.AllemandD.TambuttéS. (2011). Live tissue imaging shows reef corals elevate pH under their calcifying tissue relative to seawater.PLoS One6:e20013. 10.1371/journal.pone.0020013

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 56

  WuW.PriestC.ZhouJ.PengC.LiuH.JiangD.-E. (2016). Solvation of the Ca2UO2(CO3)3 complex in seawater from classical molecular dynamics.J. Phys. Chem. B1207227–7233. 10.1021/acs.jpcb.6b05452

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 57

  YorkD.EvensenN. M.MartínezM. L.De Basabe DelgadoJ. (2004). Unified equations for the slope, intercept, and standard errors of the best straight line.Am. J. Phys.72367–375. 10.1119/1.1632486

  • CrossRef
  • Google Scholar

• 58

  ZoccolaD.TambuttéE.KulhanekE.PuverelS.ScimecaJ.-C.AllemandD.et al (2004). Molecular cloning and localization of a PMCA P-type calcium ATPase from the coral Stylophora pistillata.Biochim. Biophys. Acta (BBA) Biomembr.1663117–126. 10.1016/j.bbamem.2004.02.010

  • Pubmed Abstract
  • CrossRef
  • Google Scholar