Estimation of dietary intake of sodium, potassium, phosphorus and protein in healthy Indian population and patients with chronic kidney disease

Introduction

The burden of non-communicable diseases (NCDs) such as hypertension, diabetes mellitus, cardiovascular disease (CVD) and chronic kidney disease (CKD) is increasing in India. According to the World Health Organization (WHO), 63% of total deaths reported in 2016 in India were due to NCDs, with 27% attributed to cardiovascular diseases (CVD) (1). Similarly, the number of deaths due to CKD is rising in India. Unhealthy diets, lack of physical activity and use of alcohol and tobacco are major NCD risk factors (2, 3). The WHO guidelines recommend a daily dietary sodium intake of 2 g (corresponding to 5 g salt) and potassium consumption of at least 3.50 g (4). In the presence of chronic conditions such as kidney diseases and hypertension, sodium intake should not be more than 1.50 g per day (5). The National Academies of Sciences, Engineering, and Medicine Dietary Reference Intakes for Sodium and Potassium lists the maximum amount of sodium consumption as 2.30 grams per day for chronic disease risk reduction (6), and the Kidney Disease Global Outcomes (KDIGO) guidelines recommend restricting sodium intake to <2 g/day (7). According to the Institute of Medicine (The National Academy of Medicine), the recommended dietary allowance (RDA) for phosphorus and protein for healthy adults is 700 mg/day and 0.80 g/kg/day (8), respectively. The actual phosphorus and protein intake in the western populations, however, is 1,056–1,617 mg/day (9–11) and 1.30–1.40 g/kg/day (12), respectively. The biological availability of protein depends on the source, with vegetarian sources (biological value 56–74%) being less efficient than animal proteins (biological value 77–104%) (13). Normative data on nutrient intake is limited in Indian populations, and where available, is based on dietary recall, which indicates a gap in knowledge (2, 14–17).

Dietary manipulation is an important strategy in the management of patients with NCDs, including CKD. In addition to salt restriction, protein intake is recommended to not exceed beyond 0.80 g/kg/day (0.55–0.60 g/kg/day for CKD stage 3–5, 0.60–0.80 g/kg/day in case of diabetic patients) to slow the progression of CKD (18). For the Western societies, where the normal dietary protein intake is 1.30 g/kg/day (12), this is commonly expressed as ‘limiting’ protein intake. The appropriateness of this recommendation in other societies with substantially different food habits has not been examined. For example, the usual protein intake in predominantly vegetarian Indian societies is likely lower (39–57 g/day) (2, 14, 16). A systematic review also highlighted the diverse dietary patterns in India, including large variations across regions and over time. For example, sweets, snacks, meat or fish were the most prevalent diets in the Eastern and Southern parts of India whereas fruits, vegetables, rice and pulses were popular in the North and West (19). Accurate assessment of dietary intake is therefore essential to develop appropriate dietary advice for people with chronic conditions. The 24-h urinary excretion method is better than dietary recall methods as recall is a retrospective diet assessment method, where an individual needs to remember their food consumption during the preceding 24 h (20). Also, the recall method is prone to errors due to literacy demand, burden, and challenges with portion size estimation (21).

The dietary protein intake is best estimated by measuring the urea nitrogen appearance rate, which requires a 24-h urine collection (22). Similarly, the estimation of the 24-h urinary sodium excretion is the gold standard for estimating salt intake (23–26), and dietary potassium intake is correlated with the 24-h urinary excretion of potassium (27, 28). Dietary assessment has also been shown to be prone to errors in estimating dietary phosphorus intake, making 24-h urine collection the optimal method for this purpose as well (29).

Information on recommended dietary allowances and actual dietary intake of nutrients are important for development of personalized dietary plans in specific population with variable health issues. There are limited studies with small sample size on healthy individuals that have examined the dietary intake of nutrients. Most of these have focused on salt intake. In this study, we aimed to determine the daily intake of sodium, potassium, protein and phosphorus using 24-h urinary excretion method in a group of healthy subjects and those with mild-to-moderate CKD in north India. Given the importance of ascertaining the sodium intake, we also evaluated the performance of equations designed to estimate dietary sodium intake using sodium concentration in a spot urine sample to help identify the equation best suited for this purpose in Indian subjects. These equations have been derived in Caucasian, Japanese or Chinese populations and use different input parameters, for example, sex and potassium (30–35). None of these were developed for Indians.

Methods

Study design and population

This cross-sectional study was conducted at the Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh, India, as a part of the study to measure the glomerular filtration rate in Indian population between 2016 and 2020. We recruited adults over the age of 18 years of either sex with stage 2 to 4 CKD [estimated glomerular filtration rate (eGFR) 15–60 mL/min/1.73m² or eGFR >60 mL/min/1.73m² and proteinuria: ≥500 mg/day] (36) and healthy volunteers drawn from the families of those with CKD. Chronic Kidney Disease-Epidemiology Collaborative Group (CKD-EPI) creatinine equation 2009 (CKD-EPI GFR 2009) was used for eGFR estimation. All healthy volunteers were confirmed to be normotensive, and had a normal HbA1C, eGFR >60 mL/min/1.73 m2, and were normoalbuminuric. Other health conditions were excluded by self-reporting. Individuals with voiding problems, urinary incontinence, limb amputation, chronic liver disease, or cardiac disease were also excluded. Figure 1 shows the flow diagram for the study subjects. The study was approved by the Institutional Ethics Committee at PGIMER, Chandigarh, and all subjects provided written consent. All the methods used in this study were performed in accordance with relevant guidelines and regulations.

Figure 1

Figure 1. Consort flow diagram for the study subjects.

Study conduct

Urine sample collection

Written and verbal instructions for 24-h urine collection were provided to all subjects. Subjects were asked to discard the first void of the day of starting the urine collection and collect all subsequent urine voids over the next 24-h period. The volume of 24-h urine samples was measured at reporting. Subjects were asked to collect a second-morning spot urine sample in a 50 mL container. Both spot and 24-h urine samples were transported to the laboratory and aliquots were drawn. Complete urine collection of 24-h urine volume of ≥500 mL was considered for analysis. Urine collection compliance was confirmed by the following formula: collected urine volume/(body weight × 21) > 0.7 (37). For collections with values lower than 0.7, we checked the 24-h urine creatinine excretion. If it was lower than the reference value (< 670 mg for men or < 450 mg for women), the data were excluded from the analysis (37). Urine samples were centrifuged at 2000 rpm for 15 min, and supernatant was collected in 2 mL vials. A total of 4 aliquots of 2 mL were stored at -80°C, out of which one was given to the biochemistry laboratory for analysis. Estimation of dietary intake using 24-h urinary excretion has been considered a reliable tool provided the compliance of 24-h urine collection has been validated which we have done carefully (22).

Demographic, anthropometric and clinical measurements

Demographic details were recorded. Weight was measured in minimum clothing using calibrated scales. Height was measured as per standard protocols using a wall mount height measurement scale. The Body Mass index (BMI) was calculated as kg/m² and categorised as BMI ≥ 25 kg/m² and BMI <25 kg/m². The blood pressure was measured using a validated Omron BP monitor following WHO protocol (38). Hypertension was defined as blood pressure ≥ 140/90 mmHg or use of antihypertensive medications. The waist and hip circumferences were measured using standard methods (39). Abdominal obesity was defined as waist circumference ≥ 90 cm in men or ≥ 80 cm in women (40). All these anthropometric measurements, along with blood pressure, was measured three times by a trained study nurse, and the mean of three values was used for analysis.

Laboratory analyses

A total 5 mL blood sample was withdrawn from each subject and collected in BD Vacutainer® tubes and serum was separated for the analysis of serum creatinine (modified Jaffe’s method traceable to isotope dilution mass spectrometry (IDMS) standard), urea, and albumin. 24-h urine samples were analysed for sodium, creatinine, protein, urea, phosphorus and potassium. Urine spot samples were analysed for sodium excretion. All investigations were done at the Central Laboratory facility, PGIMER, Chandigarh using COBAS c702 auto-analyzer (Roche Diagnostic Limited, Rotkreuz, Switzerland). The sodium and potassium were measured using Ion-Selective Electrode method, and phosphorus and protein were measured using Molybdate UV (41) and Turbidimetric methods (42) respectively.

Dietary salt intake was calculated using 24-h urine sodium value using the formula (2):

$\begin{array}{l}\mathrm{Amount}\mathrm{of}\mathrm{salt}\left(\mathrm{g}/\mathrm{day}\right)\\ =\left(\mathrm{Urinary}\mathrm{sodium}\left(\mathrm{mmol}/\mathrm{day}\right)\times 0.023\right)/0.397\end{array}$ .

The dietary protein intake per day was calculated by 24-h urine urea nitrogen method as follows.

$\begin{array}{l}\mathrm{Protein}\mathrm{intake}\left(\mathrm{g}/\mathrm{day}\right)\\ =[\left(\mathrm{urine}\mathrm{urea}\mathrm{nitrogen}\left(\mathrm{g}/\mathrm{day}\right)+\left(\mathrm{weight}\left(\mathrm{kg}\right)\times 0.031\right)]{}^{\ast }6.25\right)\end{array}$ (43, 44).

Potassium intake was calculated as:

$\begin{array}{l}\mathrm{Potassium}\mathrm{intake}\left(\mathrm{mg}/\mathrm{day}\right)=\mathrm{Urinary}\mathrm{potassium}\mathrm{excretion}\\ \left(\mathrm{mmol}/\mathrm{day}\right)\times 39\left(\mathrm{molecular}\mathrm{weight}\mathrm{of}\mathrm{potassium}\right)\end{array}$ (27, 28).

Phosphorus intake was calculated assuming an absorption rate of 65% (29).

$\begin{array}{l}\mathrm{Dietary}\mathrm{phosphorus}\mathrm{intake}\left(\mathrm{mg}/\mathrm{day}\right)\\ =\mathrm{Urinary}\mathrm{phosphorus}\mathrm{excretion}\left(\mathrm{mg}/\mathrm{day}\right)/0.65\end{array}$ .

Estimation of salt intake using spot urinary sodium concentration

We compared the measured 24 h urinary sodium excretion with those derived using seven estimation equations (Kawasaki, Tanaka, Toft, INTERSALT with and without potassium, Whitton and Mage) (30–35). Detailed formulae are presented in Appendix 1 in Supplementary Materials.

Statistical analyses

Data are presented as mean ± standard deviation or as median and 25th and 75th percentiles. The normality of data was tested using Shapiro–Wilk test. Continuous data were compared using independent t-test or Mann Whitney U test as appropriate. Categorical data were compared using the chi-square test. Correlation between variables was tested using Pearson’s or Spearman’s rho correlation as appropriate. Dietary intake of nutrients was also analysed in subgroups based on BMI, blood pressure and abdominal obesity. Mean bias for 24-h urinary sodium was computed as the difference between the measured and estimated values obtained from different equations and expressed as mean ± standard deviation (SD). The Bland–Altman Plot was used to estimate the bias and limits of agreement between sodium values obtained by 24 h urinary excretion method and sodium estimating equation methods. Limits of agreement were calculated as mean bias ±1.96 × SD. Precision was defined as 95% confidence interval of bias. Two-tailed p value <0.05 was considered significant. Data were analyzed using the Statistical Package for the Social Sciences (SPSS) software for Macintosh, version 26.0 (IBM Corp., Armonk, NY, United States).

Results

Overall, 662 individuals were screened, out of whom 430 were enrolled (Figure 1). Another 26 participants were excluded from analysis because of various reasons and finally, data from 404 participants (182 adult healthy volunteers and 222 adults with CKD) were analyzed (Figure 1). Among adults with CKD, 71 (32%) were in stage 4, 127 (57%) were in stage 3 and 24 (11%) in stage 2. The demographic characteristics of the participants are shown in Table 1. The mean age was 47.01 ± 11.46 years, with equal distribution of men and women. The mean eGFR of the study subjects was 71.10 ± 38.28 mL/min/1,73m², and the mean BMI was 24.94 ± 5.47 kg/m², with 121 (30%) being overweight (BMI ≥25 kg/m²) and 51 (12.60%) were obese (BMI ≥30 kg/m²).

Table 1

Table 1. Demographic and clinical characteristics of study population.

Table 1 shows the daily dietary nutrient intake in the study population. The mean dietary intakes of sodium, salt, potassium, protein and phosphorus were 2.94 ± 1.68 g/day, 7.42 ± 4.24 g/day, 1.43 ± 0.59 g/day, 47.67 ± 14.73 g/day and 0.86 ± 0.39 g/day, respectively. Compared to healthy subjects, those with CKD had similar dietary intakes of sodium (2.81 ± 1.60 g/day vs., 3.05 ± 1.73 g/day, p = 0.136), salt (7.08 ± 4.04 g/day vs. 7.70 ± 4.37 g/day, p = 0.136) protein (47.16 ± 14.59 g/day vs. 48.08 ± 14.86 g/day, p = 0.531), potassium (1.38 ± 0.59 g/day vs1.48 ± 0.58 g/day, p = 0.095) and phosphorus (0.86 ± 0.41 mg/day vs. 0.87 ± 0.37 g/day, p = 0.741). However, protein intake per kilogram of body weight was more in healthy subjects (0.78 ± 0.25 g/kg/day vs 0.73 ± 0.20 g/kg/day, p = 0.015). The salt consumption was above the WHO recommended intake of 5 g/day in 118 (64.83%) of the healthy population and 150 (67.56%) of those with CKD.

In general, men consumed more dietary salt; 8.28 ± 4.44 vs. 6.55 ± 3.83 g/day (p < 0.001), protein; 51.43 ± 15.74 g/day vs. 43.84 ± 12.56 (p < 0.001), potassium; 1.52 ± 0.58 vs. 1.35 ± 0.58 g/day (p = 0.003) and phosphorus; 0.94 ± 0.39 mg/day vs. 0.79 ± 0.36 g/day (p < 0.011) as compared to women (Supplementary Table S1).

Stratification based on body mass index (BMI) and blood pressure was done as shown in Table 2. A total of 80 (44%) healthy volunteers and 92 (41%) subjects with CKD were overweight, whereas 168 (76%) of those with CKD were hypertensive. Consumption of salt, potassium, protein and phosphorus was higher among subjects with BMI ≥ 25 than those with BMI < 25, i.e., 8.38 ± 4.67 vs. 6.71 ± 3.73 g/day, p < 0.001 (salt), 1.57 ± 0.63 vs. 1.33 ± 0.53 g/day, p < 0.001 (potassium), 51.12 ± 13.77 vs. 45.11 ± 14.92 g/day, p < 0.001 (protein) and 0.93 ± 0.38 vs. 0.82 ± 0.38 g/day, p = 0.005 (phosphorus), respectively. Compared to normotensive study subjects, hypertensive individuals (blood pressure ≥ 140/90 mm Hg) consumed more salt and potassium, i.e., 8.23 ± 4.89 vs. 6.84 ± 3.59 g/day (p = 0.002) and 1.51 ± 0.63 vs. 1.38 ± 0.55 g/day (p = 0.024), respectively. No differences were seen in terms of protein and phosphorus intake.

Table 2

Table 2. Daily dietary intake of nutrients per 24 h of urinary excretion as per BMI and blood pressure category in different subgroups.

Also, data were grouped based on the presence or absence of sex-specific abdominal obesity (waist circumference ≥ 90 cm in men or ≥ cm in women) (40). A total of 112 (62%) healthy individuals, and 137 (62%) of those with CKD had abdominal obesity. Overall, 115 (57%) men and 134 (68%) women had abdominal obesity. The protein intake in men and women with abdominal obesity was higher than in the non-obese (abdominal) individuals (54.33 ± 15.12 vs. 47.62 ± 15.98 g/day, p = 0.003 for men; and 45.68 ± 11.50 vs. 39.60 ± 14.05 g/day, p = 0.004 for women). However, weight-corrected protein intake was lower in men and women with abdominal obesity (0.72 ± 0.18 vs. 0.81 ± 0.28 g/kg/day, p = 0.014 for men and 0.71 ± 0.18 vs. 0.80 ± 0.27 g/kg/day, p = 0.019 for women). Intake of salt, potassium and phosphorus was significantly higher in both men and women with abdominal obesity (Table 3).

Table 3

Table 3. Daily dietary intake of nutrients as per abdominal obesity in men and women.

Table 4 and Figure 2 show the performance of equations used to estimate 24-h sodium intake from spot urinary sodium concentration against the measured 24-h urinary sodium excretion. The mean bias ranged from −47.82 (95% CI, −55.54 to −40.09) for the Kawasaki equation to 15.97 (95% CI, 9.24 to 22.69) for the Whitton formula. The INTERSALT2 formula had the least bias of all equations [1.08 (95% CI, −5.50 to 7.65)].

Table 4

Table 4. Comparison of measured and estimated 24 h urinary sodium excretion in study subjects.

Figure 2

Figure 2. Bland–Altman plots for the difference in agreement between the measured sodium (mSodium) and estimated sodium using Kawasaki (A), Tanaka (B), INTERSALT1 (C), INTERSALT1 (D), Toft (E), Whitton (F) and Mage (G) estimating equations shown by plotting the bias (measured sodium intake minus estimated sodium intake) against the mean of measured and estimated sodium intake. Central line represent the mean bias between measured and estimated methods and dotted line represent ±1.96 SD.

Discussion

This is the first study to provide a comprehensive assessment of the intake of multiple nutrients in the north Indian population that included healthy subjects and those with CKD. We document a higher-than-recommended dietary intake of salt and phosphorus and relatively low protein and potassium consumption.

The dietary salt intake was higher than recommended in about two-thirds of the study subjects, with no heterogeneity with regard to sex, or presence of hypertension or CKD (Figure 3). While this is the first study looking at salt intake in subjects with CKD, a similar finding has been reported in general population studies conducted in different parts of India. Johnson et al., using 24-h estimation, found the dietary salt intake to be higher among the population residing in Andhra Pradesh (9.46 g/day) compared to people of Delhi and Haryana (8.59 g/day) (17). A nationally representative cross-sectional survey conducted in 2017–18 in India estimated the salt intake to be 8 g/day using the INTERSALT equation on spot urine samples (45). The somewhat lower salt intake amongst subjects in this study compared to those from older studies could be attributed to increased awareness about the benefit of salt reduction and human health, in particular the targeted advice received by patients with CKD, which might have also influenced the consumption in other members of the family from whom the healthy population was drawn. Although the direction of movement is encouraging, a majority of the population (including those with CKD) is still consuming higher than recommended amounts of salt, indicating an unfinished public health agenda. This might also indicate the limits to which salt reduction is possible only with education. A study from China also reported the consumption of similar amounts of salt (7.40 g/day) among patients with CKD (46). Together, these findings raise the need to consider alternate interventions, such as low-sodium salt substitutes. Concerns are often raised about the high potassium content of salt substitutes. While these may be valid for those with advanced stages of CKD, large population-based studies (47) have not shown an increased risk of hyperkalemia. Given the remarkable ability of the kidneys to excrete potassium until the late stages of CKD, it is unlikely that use of salt substitutes will pose a substantial risk of hyperkalemia at a population level (48). Appropriate caution, however, should be exercised in those with advances stages of CKD.

Figure 3

Figure 3. Salt intake per day in various subgroups. Data represented as median (25th, 75th percentile). HV; healthy volunteer, CKD; chronic kidney disease.

Also striking was the low potassium intake in the study population, not only amongst those with CKD but also in the otherwise healthy population. The intake was substantially lower than 3.50 g/day recommended by the WHO for the healthy population and 2-4 g for those with early stages of CKD as per KDOQI 2020 guidelines (49). Given that nuts, green vegetables, and fruits are the main sources of potassium in the diet, the finding reflects low dietary diversity in our population (50). Our findings are consistent with the results of a 32-country INTERSTROKE study that found overall low urinary potassium excretion (1.68 g/day) in 32 countries, including India (15), and other Indian studies (50, 51). Studies from West Africa (1.8 g/day) (52) and China (1.5 g/day) (53) reported potassium intake similar to the current study. The combination of higher than recommended sodium and lower than recommended potassium collectively intake raises cardiovascular disease risk.

Another notable finding was the relatively low dietary protein intake, especially in the healthy individuals (0.78 g/kg). Previous studies from different parts of India that included healthy people and those with hypertension, CKD and diabetes have shown similar findings (Table 5). Protein intake measured using 24-h or 3-day dietary recall and or a validated Food Frequency Questionnaire (FFQ) in healthy individuals varies from 39.54 g/day to 67.5 g/day (2, 14, 16, 58), whereas protein intake in subjects with CKD was 39.05 g/day (2, 14, 16, 58). This finding is of relevance with regard to the CKD population. Current guidelines recommend restricting dietary protein intake to about 0.8 g/kg/day in those with CKD stage 1–2 and 0.55–0.60 g/kg/day in those with CKD Stage 3–5 (17) to retard disease progression. Data from Western countries show a wide variation in protein intake. A Danish survey reported protein intake ranging from 75.5–94.2 g/day among men and 59.1–69.7 g/day among women (59). A multinational (Denmark, Finland, Netherlands, United Kingdom, Spain, Bulgaria, Australia, New Zealand) randomised controlled trial in prediabetic obese individuals showed a protein intake of 105.8 ± 35.7 g/day (60, 61). The Nurses’ Health Study from the US reported a mean protein intake of 76 grams both in subjects with normal renal function and with mild renal insufficiency (62). The EPIC-Oxford cohort of the general population, which included meat eaters, fish eaters, vegetarians, and vegans, also showed high protein intakes (0.99 to 1.28 g/kg/day) (63). Therefore in the Western context, restriction of dietary protein intake is advised. However, this advice is interpreted in other parts of the world without contextualizing the prevalent dietary practice. Further, excessive protein restriction is likely to lead to protein-energy wasting, a phenomenon shown to be associated with poorer outcomes in those with CKD (64).

Table 5

Table 5. Studies showing the dietary intake of protein, salt, potassium and phosphorus in Indians.

Regarding phosphorus intake, the mean intake was above the recommended levels. Usually, the dietary phosphorus intake tracks with the protein in the western meat-based diets, but there may be a divergence in India, where dairy products are the main source of phosphorus and a reduced phosphorus intake in those with CKD could indicate preferential reduction of dairy products. Finally, doubts have been raised about the reliability of 24-h urinary phosphate for estimating dietary intake in the presence of CKD (65).

We have summarised findings from published studies on dietary intakes of multiple nutrients across India including healthy and individuals with various NCDs (Table 5) (2, 14, 16, 17, 45, 50, 54–57).

The salt, protein, potassium and phosphorus intakes were higher among subjects with higher BMI than those with normal BMI, consistent results with earlier findings (66–70), suggesting overall better nourishment. In line with results as per BMI, both men and women subjects with abdominal obesity has higher intake of all these nutrients. We also noted some sex-specific differences in dietary intake of salt, protein, potassium, and phosphorus. All of them were significantly lower in females. These findings are consistent with previous studies, and indicate a comparative nutritional disadvantage for females, except for salt.

Finally, we analyzed the performance of the estimated 24-h sodium consumption by comparing it with 24-h urine sodium excretion levels. Our data shows that the INTERSALT2 exhibited the least bias. Hence, we suggest the use of this equation to estimate sodium intake using spot urine samples. Whitton et al. also found the INTERSALT equation suitable for estimating sodium excretion in urban Asian populations (Singapore residents of Chinese, Malay, and Indian ethnicity) (30). One possible reason this equation performed better in our population is that it was developed using data from 32 countries that included Indian subjects in contrast to others that did not include Indian subjects. A recent study, however, found poor agreement between the actual sodium intake and the estimated intake using all equations, suggesting a high degree of variability and the need to use data from estimation equations with caution (71).

The strength of this study is the inclusion of a well-phenotyped population, including healthy subjects and those with CKD and the use of 24-h urine excretions for analysis of nutrient intake. Our study had a few limitations, including a relatively small sample size, lack of adjustments for dietary energy intake, lack of dietary assessment over a period of time and lack of a community-based study sample as the study was conducted at the hospital level. We also did not correct for the faecal loss of potassium and phosphorus. This limits the value of using urinary excretion as a surrogate for intake. Further, The enrolment of healthy subjects from amongst the family members of subjects with CKD might have introduced a bias since they could have altered their dietary habits as well. Therefore, our findings remain hypothesis-generating for the general population and should be confirmed in larger population-based samples.

In conclusion, the study shows that intake of several nutrients, including salt, potassium, phosphorus and protein, is either above or below the recommended levels, which might be one reason for increased NCD risk, including CKD. An improved understanding of dietary patterns and nutrient intakes will help provide tailored dietary advice to persons with various health conditions, including CKD. These data can help support the development of locally appropriate guidelines and implementation strategies such as public awareness, counselling at the individual level, and developing appropriate food policy.

Data availability statement

The original contributions presented in the study are included in the article/Supplementary material, further inquiries can be directed to the corresponding authors.

Ethics statement

The studies involving humans were approved by Postgraduate Institute of Medical Education and Research, Chandigarh, India. The studies were conducted in accordance with the local legislation and institutional requirements. The participants provided their written informed consent to participate in this study.

Author contributions

PK: Data curation, Methodology, Writing – original draft. AY: Conceptualization, Data curation, Formal analysis, Funding acquisition, Methodology, Project administration, Supervision, Validation, Writing – original draft, Writing – review & editing. AP: Investigation, Methodology, Writing – review & editing. RJ: Investigation, Writing – review & editing. NuS: Methodology, Supervision, Resources, Writing – review & editing. NaS: Methodology, Data curation, Writing – review & editing. VK: Funding acquisition, Investigation, Data curation, Resources, Supervision, Writing – review & editing. VJ: Conceptualization, Data curation, Funding acquisition, Resources, Supervision, Writing – review & editing.

Funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. We acknowledge the grant received by Department of Biotechnology, New Delhi, India (grant no: BT/PR11105/MED/30/1345/2014), Science and Engineering Research Board, New Delhi, India (EMR/2017/002159), and Department of Biotechnology-Wellcome Trust, India Alliance (IA/CPHI/19/1/504609) to support this study. Open access fee was paid from the Imperial College London Open Access Fund.

Conflict of interest

VJ has received grant funding from GSK, Baxter Healthcare, and Biocon and honoraria from Bayer, AstraZeneca, Boeringer Ingelheim, NephroPlus and Zydus Cadilla, under the policy of all honoraria being paid to the organization.

The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Supplementary material

The Supplementary material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fnut.2024.1312581/full#supplementary-material

References

1. World Health Organization. Cardiovascular diseases Available at: https://www.who.int/india/health-topics/cardiovascular-diseases (Accessed on October 4, 2023)

Google Scholar

2. Smina, TP, Kumpatla, S, and Viswanathan, V. Higher dietary salt and inappropriate proportion of macronutrients consumption among people with diabetes and other co morbid conditions in South India: estimation of salt intake with a formula. Diabetes Metab Syndr. (2019) 13:2863–8. doi: 10.1016/j.dsx.2019.07.059

PubMed Abstract | Crossref Full Text | Google Scholar

3. World Health Organization. Global action plan for the prevention and control of NCDs 2013–2020 2013-2020 Available at: https://www.who.int/publications/i/item/9789241506236. (Accessed on October 4, 2023)

Google Scholar

4. World Health Organization. WHO issues new guidance on dietary salt and potassium 2013 Available at: https://www.who.int/news/item/31-01-2013-who-issues-new-guidance-on-dietary-salt-and-potassium#:~:text=Adults%20should%20consume%20less%20than,guidelines%20issued%20by%20the%20WHO. (Accessed on October 4, 2023)

Google Scholar

5. National Institute of Diabetes and Digestive and Kidney Diseases. Diet & Nutrition for adults with advanced chronic kidney Disease Available at: https://www.niddk.nih.gov/health-information/kidney-disease/chronic-kidney-disease-ckd/eating-nutrition/nutrition-advanced-chronic-kidney-disease-adults. (Accessed on October 4, 2023)

Google Scholar

6. National Academies of sciences E, and medicine. Sodium and potassium dietary reference intake values updated in new report; introduces new category for sodium based on chronic Disease risk reduction 2019 Available at: https://www.nationalacademies.org/news/2019/03/sodium-and-potassium-dietary-reference-intake-values-updated-in-new-report#:~:text=For%20individuals%20ages%2014%20and,for%20children%20ages%201%2D13. (Accessed on October 4, 2023)

Google Scholar

7. Kidney disease: improving global outcomes blood pressure work Group. KDIGO 2021 clinical practice guideline for the Management of Blood Pressure in chronic kidney Disease. Kidney Int. (2021) 99:S1–S87. doi: 10.1016/j.kint.2020.11.003

Crossref Full Text | Google Scholar

8. Institute of Medicine. Phosphorus Available at: https://ods.od.nih.gov/factsheets/Phosphorus-HealthProfessional/. (Accessed on October 4, 2023)

Google Scholar

9. Itkonen, ST, Karp, HJ, Kemi, VE, Kokkonen, EM, Saarnio, EM, Pekkinen, MH, et al. Associations among total and food additive phosphorus intake and carotid intima-media thickness—a cross-sectional study in a middle-aged population in southern Finland. Nutr J. (2013) 12:94. doi: 10.1186/1475-2891-12-94

PubMed Abstract | Crossref Full Text | Google Scholar

10. Olivo, RE, Hale, SL, Diamantidis, CJ, Bhavsar, NA, Tyson, CC, Tucker, KL, et al. Dietary phosphorus and ambulatory blood pressure in African Americans: the Jackson heart Study. Am J Hypertens. (2019) 32:94–103. doi: 10.1093/ajh/hpy126

PubMed Abstract | Crossref Full Text | Google Scholar

11. Gutierrez, OM, Porter, AK, Viggeswarapu, M, Roberts, JL, and Beck, GR Jr. Effects of phosphorus and calcium to phosphorus consumption ratio on mineral metabolism and cardiometabolic health. J Nutr Biochem. (2020) 80:108374. doi: 10.1016/j.jnutbio.2020.108374

PubMed Abstract | Crossref Full Text | Google Scholar

12. Mariotti, F, and Gardner, CD. Dietary Protein and Amino Acids in Vegetarian Diets-A Review. Nutrients. (2019) 11. doi: 10.3390/nu11112661

PubMed Abstract | Crossref Full Text | Google Scholar

13. Berrazaga, I, Micard, V, Gueugneau, M, and Walrand, S. The role of the anabolic properties of plant- versus animal-based protein sources in supporting muscle mass maintenance: a critical review. Nutrients. (2019) 11. doi: 10.3390/nu11081825

PubMed Abstract | Crossref Full Text | Google Scholar

14. Kumar, A, Mittal, S, Orito, S, Ishitani, K, and Ohta, H. Impact of dietary intake, education, and physical activity on bone mineral density among north Indian women. J Bone Miner Metab. (2010) 28:192–201. doi: 10.1007/s00774-009-0118-y

PubMed Abstract | Crossref Full Text | Google Scholar

15. Judge, C, O'Donnell, MJ, Hankey, GJ, Rangarajan, S, Chin, SL, Rao-Melacini, P, et al. Urinary sodium and potassium, and risk of ischemic and hemorrhagic stroke (INTERSTROKE): a case-control Study. Am J Hypertens. (2021) 34:414–25. doi: 10.1093/ajh/hpaa176

PubMed Abstract | Crossref Full Text | Google Scholar

16. Joshi, SR, Bhansali, A, Bajaj, S, Banzal, SS, Dharmalingam, M, Gupta, S, et al. Results from a dietary survey in an Indian T2DM population: a STARCH study. BMJ Open. (2014) 4:e005138. doi: 10.1136/bmjopen-2014-005138

PubMed Abstract | Crossref Full Text | Google Scholar

17. Johnson, C, Mohan, S, Rogers, K, Shivashankar, R, Thout, SR, Gupta, P, et al. Mean dietary salt intake in urban and rural areas in India: a population survey of 1395 persons. J Am Heart Assoc. (2017) 6. doi: 10.1161/JAHA.116.004547

PubMed Abstract | Crossref Full Text | Google Scholar

18. Ikizler, TA, Burrowes, JD, Byham-Gray, LD, Campbell, KL, Carrero, JJ, Chan, W, et al. KDOQI clinical practice guideline for nutrition in CKD: 2020 update. Am J Kidney Dis. (2020) 76:S1–S107. doi: 10.1053/j.ajkd.2020.05.006

PubMed Abstract | Crossref Full Text | Google Scholar

19. Green, R, Milner, J, Joy, EJ, Agrawal, S, and Dangour, AD. Dietary patterns in India: a systematic review. Br J Nutr. (2016) 116:142–8. doi: 10.1017/S0007114516001598

PubMed Abstract | Crossref Full Text | Google Scholar

20. Raina, SK . Limitations of 24-hour recall method: micronutrient intake and the presence of the metabolic syndrome. N Am J Med Sci. (2013) 5:498. doi: 10.4103/1947-2714.117329

PubMed Abstract | Crossref Full Text | Google Scholar

21. Block, G . Human dietary assessment: methods and issues. Prev Med. (1989) 18:653–60. doi: 10.1016/0091-7435(89)90036-4

Crossref Full Text | Google Scholar

22. Abdel-Nabey, M, Saint-Jacques, C, Boffa, JJ, Frochot, V, Livrozet, M, Daudon, M, et al. 24-h urine collection: a relevant tool in CKD nutrition evaluation. Nutrients. (2020) 12. doi: 10.3390/nu12092615

PubMed Abstract | Crossref Full Text | Google Scholar

23. Campbell, NRC, He, FJ, Tan, M, Cappuccio, FP, Neal, B, Woodward, M, et al. The international consortium for Quality research on dietary sodium/salt (TRUE) position statement on the use of 24-hour, spot, and short duration (<24 hours) timed urine collections to assess dietary sodium intake. J Clin Hypertens (Greenwich). (2019) 21:700–9. doi: 10.1111/jch.13551

Crossref Full Text | Google Scholar

24. Ji, C, Sykes, L, Paul, C, Dary, O, Legetic, B, Campbell, NR, et al. Systematic review of studies comparing 24-hour and spot urine collections for estimating population salt intake. Rev Panam Salud Publica. (2012) 32:307–15. doi: 10.1590/S1020-49892012001000010

PubMed Abstract | Crossref Full Text | Google Scholar

25. Lucko, AM, Doktorchik, C, Woodward, M, Cogswell, M, Neal, B, Rabi, D, et al. Percentage of ingested sodium excreted in 24-hour urine collections: a systematic review and meta-analysis. J Clin Hypertens (Greenwich). (2018) 20:1220–9. doi: 10.1111/jch.13353

PubMed Abstract | Crossref Full Text | Google Scholar

26. Cogswell, ME, Maalouf, J, Elliott, P, Loria, CM, Patel, S, and Bowman, BA. Use of urine biomarkers to assess sodium intake: challenges and opportunities. Annu Rev Nutr. (2015) 35:349–87. doi: 10.1146/annurev-nutr-071714-034322

PubMed Abstract | Crossref Full Text | Google Scholar

27. Dennis, B, Stamler, J, Buzzard, M, Conway, R, Elliott, P, Moag-Stahlberg, A, et al. INTERMAP: the dietary data—process and quality control. J Hum Hypertens. (2003) 17:609–22. doi: 10.1038/sj.jhh.1001604

PubMed Abstract | Crossref Full Text | Google Scholar

28. Taylor, EN, Stampfer, MJ, Mount, DB, and Curhan, GC. DASH-style diet and 24-hour urine composition. Clin J Am Soc Nephrol. (2010) 5:2315–22. doi: 10.2215/CJN.04420510

PubMed Abstract | Crossref Full Text | Google Scholar

29. Morimoto, Y, Sakuma, M, Ohta, H, Suzuki, A, Matsushita, A, Umeda, M, et al. Estimate of dietary phosphorus intake using 24-h urine collection. J Clin Biochem Nutr. (2014) 55:62–6. doi: 10.3164/jcbn.14-15

PubMed Abstract | Crossref Full Text | Google Scholar

30. Whitton, C, Gay, GM, Lim, RB, Tan, LW, Lim, WY, and van Dam, RM. Evaluation of equations for predicting 24-hour urinary sodium excretion from casual urine samples in Asian adults. J Nutr. (2016) 146:1609–15. doi: 10.3945/jn.116.232108

PubMed Abstract | Crossref Full Text | Google Scholar

31. Toft, U, Cerqueira, C, Andreasen, AH, Thuesen, BH, Laurberg, P, Ovesen, L, et al. Estimating salt intake in a Caucasian population: can spot urine substitute 24-hour urine samples? Eur J Prev Cardiol. (2014) 21:1300–7. doi: 10.1177/2047487313485517

Crossref Full Text | Google Scholar

32. Tanaka, T, Okamura, T, Miura, K, Kadowaki, T, Ueshima, H, Nakagawa, H, et al. A simple method to estimate populational 24-h urinary sodium and potassium excretion using a casual urine specimen. J Hum Hypertens. (2002) 16:97–103. doi: 10.1038/sj.jhh.1001307

PubMed Abstract | Crossref Full Text | Google Scholar

33. Mage, DT, Allen, RH, and Kodali, A. Creatinine corrections for estimating children's and adult's pesticide intake doses in equilibrium with urinary pesticide and creatinine concentrations. J Expo Sci Environ Epidemiol. (2008) 18:360–8. doi: 10.1038/sj.jes.7500614

PubMed Abstract | Crossref Full Text | Google Scholar

34. Kawasaki, T, Itoh, K, Uezono, K, and Sasaki, H. A simple method for estimating 24 h urinary sodium and potassium excretion from second morning voiding urine specimen in adults. Clin Exp Pharmacol Physiol. (1993) 20:7–14. doi: 10.1111/j.1440-1681.1993.tb01496.x

PubMed Abstract | Crossref Full Text | Google Scholar

35. Brown, IJ, Dyer, AR, Chan, Q, Cogswell, ME, Ueshima, H, Stamler, J, et al. Estimating 24-hour urinary sodium excretion from casual urinary sodium concentrations in Western populations: the INTERSALT study. Am J Epidemiol. (2013) 177:1180–92. doi: 10.1093/aje/kwt066

PubMed Abstract | Crossref Full Text | Google Scholar

36. Chapter 1: Definition and classification of CKD. Kidney Int Suppl (2011). (2013) 3:19–62. doi: 10.1038/kisup.2012.64

Crossref Full Text | Google Scholar

37. Blackburn, GL, Bistrian, BR, Maini, BS, Schlamm, HT, and Smith, MF. Nutritional and metabolic assessment of the hospitalized patient. JPEN J Parenter Enteral Nutr. (1977) 1:11–21. doi: 10.1177/014860717700100101

Crossref Full Text | Google Scholar

38. World Health Organization. WHO technical specifications for automated non-invasive blood pressure measuring devices with cuff 2020 Available at: https://www.who.int/docs/default-source/searo/indonesia/who-tech-spec-for-automated-non-invasive-blood-pressure-measuring-devices-with-cuff.pdf?sfvrsn=b112be47_2. (Accessed on October 4, 2023)

Google Scholar

39. World Health Organization. Waist circumference and waist–hip ratio: Report of a WHO expert consultation 2008 Available at: http://apps.who.int/iris/bitstream/handle/10665/44583/9789241501491_eng.pdf;jsessionid=929929DB3B3D25FCAB721EA4272618A3?sequence=1. (Accessed on October 4, 2023)

Google Scholar

40. Pradeepa, R, Anjana, RM, Joshi, SR, Bhansali, A, Deepa, M, Joshi, PP, et al. Prevalence of generalized & abdominal obesity in urban & rural India—the ICMR-INDIAB Study (phase-I) [ICMR-NDIAB-3]. Indian J Med Res. (2015) 142:139–50. doi: 10.4103/0971-5916.164234

PubMed Abstract | Crossref Full Text | Google Scholar

41. Henry, RJ . Clinical chemistry: principles and technics. 2nd ed. Hagerstown, MD: Harper and Row (1974). 723 p.

Google Scholar

42. Iwata, J, and Nishikaze, O. New micro-turbidimetric method for determination of protein in cerebrospinal fluid and urine. Clin Chem. (1979) 25:1317–9. doi: 10.1093/clinchem/25.7.1317

PubMed Abstract | Crossref Full Text | Google Scholar

43. Maroni, BJ, Steinman, TI, and Mitch, WE. A method for estimating nitrogen intake of patients with chronic renal failure. Kidney Int. (1985) 27:58–65. doi: 10.1038/ki.1985.10

Crossref Full Text | Google Scholar

44. Masud, T, Manatunga, A, Cotsonis, G, and Mitch, WE. The precision of estimating protein intake of patients with chronic renal failure. Kidney Int. (2002) 62:1750–6. doi: 10.1046/j.1523-1755.2002.00606.x

Crossref Full Text | Google Scholar

45. Mathur, P, Kulothungan, V, Leburu, S, Krishnan, A, Chaturvedi, HK, Salve, HR, et al. National noncommunicable disease monitoring survey (NNMS) in India: estimating risk factor prevalence in adult population. PLoS One. (2021) 16:e0246712. doi: 10.1371/journal.pone.0246712

PubMed Abstract | Crossref Full Text | Google Scholar

46. Sun, L, Duan, S, Zuo, C, Sun, Z, Nie, G, Zhang, C, et al. Characteristics of urinary sodium excretion in patients with chronic kidney disease in Jiangsu. China J Clin Hypertens (Greenwich). (2021) 23:1372–9. doi: 10.1111/jch.14307

PubMed Abstract | Crossref Full Text | Google Scholar

47. Neal, B, Wu, Y, Feng, X, Zhang, R, Zhang, Y, Shi, J, et al. Effect of salt substitution on cardiovascular events and death. N Engl J Med. (2021) 385:1067–77. doi: 10.1056/NEJMoa2105675

PubMed Abstract | Crossref Full Text | Google Scholar

48. Greer, RC, Marklund, M, Anderson, CAM, Cobb, LK, Dalcin, AT, Henry, M, et al. Potassium-enriched salt substitutes as a means to lower blood pressure: benefits and risks. Hypertension. (2020) 75:266–74. doi: 10.1161/HYPERTENSIONAHA.119.13241

PubMed Abstract | Crossref Full Text | Google Scholar

49. Kidney Disease Outcomes Quality I. K/DOQI clinical practice guidelines on hypertension and antihypertensive agents in chronic kidney disease. Am J Kidney Dis. (2004) 43:11–3. doi: 10.1053/j.ajkd.2004.03.003

Crossref Full Text | Google Scholar

50. Anand, S, Shivashankar, R, Kondal, D, Garg, V, Khandelwal, S, Gupta, R, et al. Potassium intake in India: opportunity for mitigating risks of high-sodium diets. Am J Prev Med. (2020) 58:302–12. doi: 10.1016/j.amepre.2019.09.017

PubMed Abstract | Crossref Full Text | Google Scholar

51. Jan, RA, Shah, S, Saleem, SM, Waheed, A, Mufti, S, Lone, MA, et al. Sodium and potassium excretion in normotensive and hypertensive population in Kashmir. J Assoc Physicians India. (2006) 54:22–6.

PubMed Abstract | Google Scholar

52. Mizehoun-Adissoda, C, Houinato, D, Houehanou, C, Chianea, T, Dalmay, F, Bigot, A, et al. Dietary sodium and potassium intakes: data from urban and rural areas. Nutrition. (2017) 33:35–41. doi: 10.1016/j.nut.2016.08.007

PubMed Abstract | Crossref Full Text | Google Scholar

53. Du, X, Fang, L, Xu, J, Chen, X, Bai, Y, and Zhong, J. Association between 24-h urinary sodium and potassium excretion and blood pressure among Chinese adults aged 18-69 years. Sci Rep. (2021) 11:3474. doi: 10.1038/s41598-021-83049-8

PubMed Abstract | Crossref Full Text | Google Scholar

54. Thakur, JS, Jeet, G, Nangia, R, Singh, D, Grover, S, Lyngdoh, T, et al. Non-communicable diseases risk factors and their determinants: a cross-sectional state-wide STEPS survey, Haryana, North India. PLoS One. (2019) 14:e0208872. doi: 10.1371/journal.pone.0208872

PubMed Abstract | Crossref Full Text | Google Scholar

55. Kumbla, D, Dharmalingam, M, Dalvi, K, Ray, S, Shah, MK, Gupta, S, et al. A Study of salt and fat consumption pattern in regional Indian diet among hypertensive and dyslipidemic PaTients-SCRIPT study. J Assoc Physicians India. (2016) 64:47–54.

PubMed Abstract | Google Scholar

56. Radhika, G, Sathya, RM, Sudha, V, Ganesan, A, and Mohan, V. Dietary salt intake and hypertension in an urban south Indian population—[CURES-53]. J Assoc Physicians India. (2007) 55:405–11.

PubMed Abstract | Google Scholar

57. Berkemeyer, S, Bhargava, A, and Bhargava, U. Urinary phosphorus rather than urinary calcium possibly increases renal stone formation in a sample of Asian Indian, male stone-formers. Br J Nutr. (2007) 98:1224–8. doi: 10.1017/S0007114507778686

PubMed Abstract | Crossref Full Text | Google Scholar

58. Swaminathan, S, Vaz, M, and Kurpad, AV. Protein intakes in India. Br J Nutr. (2012) 108:S50–8. doi: 10.1017/S0007114512002413

Crossref Full Text | Google Scholar

59. Kristensen, NB, Madsen, ML, Hansen, TH, Allin, KH, Hoppe, C, Fagt, S, et al. Intake of macro- and micro-nutrients in Danish vegans. Nutr J. (2015) 14:115. doi: 10.1186/s12937-015-0103-3

PubMed Abstract | Crossref Full Text | Google Scholar

60. Moller, G, Rikardt Andersen, J, Ritz, C, PS, M, Navas-Carretero, S, Jalo, E, et al. Higher protein intake is not associated with decreased kidney function in pre-diabetic older adults following a one-year intervention-a preview sub-Study. Nutrients. (2018) 10. doi: 10.3390/nu10010054

PubMed Abstract | Crossref Full Text | Google Scholar

61. Cirillo, M, Lombardi, C, Chiricone, D, De Santo, NG, Zanchetti, A, and Bilancio, G. Protein intake and kidney function in the middle-age population: contrast between cross-sectional and longitudinal data. Nephrol Dial Transplant. (2014) 29:1733–40. doi: 10.1093/ndt/gfu056

PubMed Abstract | Crossref Full Text | Google Scholar

62. Knight, EL, Stampfer, MJ, Hankinson, SE, Spiegelman, D, and Curhan, GC. The impact of protein intake on renal function decline in women with normal renal function or mild renal insufficiency. Ann Intern Med. (2003) 138:460–7. doi: 10.7326/0003-4819-138-6-200303180-00009

Crossref Full Text | Google Scholar

63. Sobiecki, JG, Appleby, PN, Bradbury, KE, and Key, TJ. High compliance with dietary recommendations in a cohort of meat eaters, fish eaters, vegetarians, and vegans: results from the European prospective investigation into Cancer and nutrition-Oxford study. Nutr Res. (2016) 36:464–77. doi: 10.1016/j.nutres.2015.12.016

PubMed Abstract | Crossref Full Text | Google Scholar

64. Kopple, JD, Levey, AS, Greene, T, Chumlea, WC, Gassman, JJ, Hollinger, DL, et al. Effect of dietary protein restriction on nutritional status in the Modification of Diet in Renal Disease Study. Kidney Int. (1997) 52:778–91. doi: 10.1038/ki.1997.395

PubMed Abstract | Crossref Full Text | Google Scholar

65. Stremke, ER, McCabe, LD, McCabe, GP, Martin, BR, Moe, SM, Weaver, CM, et al. Twenty-four-hour urine phosphorus as a biomarker of dietary phosphorus intake and absorption in CKD: a secondary analysis from a controlled Diet balance Study. Clin J Am Soc Nephrol. (2018) 13:1002–12. doi: 10.2215/CJN.00390118

PubMed Abstract | Crossref Full Text | Google Scholar

66. Ravi, S, Bermudez, OI, Harivanzan, V, Kenneth Chui, KH, Vasudevan, P, Must, A, et al. Sodium intake, blood pressure, and dietary sources of sodium in an adult south Indian population. Ann Glob Health. (2016) 82:234–42. doi: 10.1016/j.aogh.2016.02.001

Crossref Full Text | Google Scholar

67. Nair, S, and Bandyopadhyay, S. Sodium intake pattern in west Indian population. Indian J Community Med. (2018) 43:67–71. doi: 10.4103/ijcm.IJCM_116_17

PubMed Abstract | Crossref Full Text | Google Scholar

68. Menyanu, EK, Corso, B, Minicuci, N, Rocco, I, Russell, J, Ware, LJ, et al. Salt and potassium intake among adult Ghanaians: WHO-SAGE Ghana wave 3. BMC Nutr. (2020) 6:54. doi: 10.1186/s40795-020-00379-y

PubMed Abstract | Crossref Full Text | Google Scholar

69. Sari, DW, Noguchi-Watanabe, M, Sasaki, S, Sahar, J, and Yamamoto-Mitani, N. Estimation of sodium and potassium intakes assessed by two 24-hour urine collections in a city of Indonesia. Br J Nutr. (2021) 126:1537–48. doi: 10.1017/S0007114521000271

PubMed Abstract | Crossref Full Text | Google Scholar

70. Yamamoto, KT, Robinson-Cohen, C, de Oliveira, MC, Kostina, A, Nettleton, JA, Ix, JH, et al. Dietary phosphorus is associated with greater left ventricular mass. Kidney Int. (2013) 83:707–14. doi: 10.1038/ki.2012.303

PubMed Abstract | Crossref Full Text | Google Scholar

71. Lobene, AJ, Stremke, ER, McCabe, GP, Moe, SM, Moorthi, RN, and Hill Gallant, KM. Spot urine samples to estimate Na and K intake in patients with chronic kidney Disease and healthy adults: a secondary analysis from a controlled feeding Study. J Ren Nutr. (2021) 31:602–10. doi: 10.1053/j.jrn.2020.09.007

PubMed Abstract | Crossref Full Text | Google Scholar