Risk factors of early death in pediatric hemophagocytic lymphohistocytosis: Retrospective cohort study

Abstract

Background:

Hemophagocytic lymphocytosis (HLH) is a rare life-threatening hyperinflammatory syndrome in which early mortality remains high in patients with HLH.

Methods:

We retrospectively collected the medical records of all pediatric patients diagnosed with HLH at the West China Second Hospital of Sichuan University between January 2014 and December 2020. Collect demographic, laboratory, clinical, genetic profiles, treatment information and perform statistical analysis from records. Risk factors for death 30 days after admission were evaluated using a multivariable logistic regression model.

Results:

A total of 110 pediatric HLH patients were enrolled. The median age of patients was 44 months (IQR 23-100.5) and 62 (56.4%) in males. The 30-day mortality rate for admission to this cohort was 34 (30.9%). Multivariate logistic regression analysis showed that heart failure (OR = 13.389, 95% CI, 1.671–107.256, p = 0.015) and hypoproteinemia (OR = 4.841, 95% CI, 1.282–18.288, p = 0.020) were associated with increased early mortality in children with HLH.

Conclusions:

These identified risk factors may help clinicians stratify patients with HLH and develop targeted treatment strategies. More research is needed to explore the best treatment strategies for patients with HLH to reduce early mortality in patients with HLH.

Introduction

Hemophagocytic lymphocytosis (HLH) is a rare hyper-inflammatory syndrome characterized by an overactive immune system leading to hypercytokinemia and multi-organ dysfunction. HLH is divided into primary HLH (pHLH), which is due to genetic defects and usually familial, and secondary HLH (sHLH), which is due to infection (e.g., Epstein-Barr virus, EBV), tumors and other underlying factors (1–4). Both can be detected at any age and can be life-threatening if left untreated and effective (3, 5).

Clinical features of HLH include persistent hyperthermia, splenomegaly, cytopenia, hypertriglyceridemia, hyperferritinemia, and elevated levels of soluble CD25 (sIL-2r) (6, 7). In addition, the central nervous system (CNS) is often affected, manifested by epilepsy and cognitive dysfunction (8, 9). Laboratory tests include specific bone marrow examination and nonspecific expression of high cytokines (e.g., interleukin-6 [IL-6], interleukin-12 [IL-12], and tumor necrosis factor-a [TNF-a]) (10). Diagnosis of HLH is not difficult, but needs to be differentiated from other highly inflammatory diseases. It is important to note that chemotherapy and/or immunotherapy are often required due to the difficulty of distinguishing between pHLH and sHLH at the beginning of the diagnosis (11). In our cohort, the majority of patients belong to sHLH (especially sHLH due to EBV infection).

About 80 years ago, Scott and Robb-Smith first described the disease (12). In the time that followed, more HLH was reported, however, HLH was notorious for its high mortality rate. For example, about 10 years ago, there were reports that the 180-day survival rate for sHLH was only 26% (13). Happily, with the continuous improvement of diagnosis and treatment options, its long-term survival rate has improved significantly. Recently, in a cohort study of HLH patients in Yoon et al., the 5-year survival rates for sHLH induced by different etiologies (e.g., EBV infection, autoimmune disease, other infections, or unexplained causes) were 25.1%, 82.4%, 78.7%, and 55.5%, respectively (14). Still, the early high mortality rate of HLH is significant, and it may be of great significance that research can predict risk factors for early death from HLH. That's why we conducted this study. By reviewing the medical records of a tertiary children's hospital in southwest China and following up HLH patients included in the study, we described the clinical parameters of these patients and analyzed the risk factors associated with 30-day mortality.

Materials and methods

Patients

This is a retrospective cohort study. Data collection and graphical analysis took place between March 2022 and July 2022. We reviewed all children with HLH in the Information Management System (HIS) of West China Second Hospital of Sichuan University between January 2014 and December 2020. The inclusion criteria were: (1) age less than 18 years, (2) hospitalized patients (ICD-10) code D76.1 (hemophagocytic lymphohistocytosis) or D76.2 (hemophagocytic syndrome, infection-related). Exclusion criteria were: (1) children who did not meet the HLH-2004 diagnostic criteria, (2) not a first admission, (3) were incomplete, (4) lost follow-up, and (5) children who had been hospitalized for less than 24 h.

Definitions

HLH diagnostic criteria confirmed using HLH-2004 (6, 15). Briefly, molecular diagnostics that are HLH compliant (e.g., PRF1, UNC13D, STX11, STXBP2, Rab27α, SH2D1A, BIRC4) and/or meet 5 of the following first 7 diagnostic criteria (due to the availability of soluble CD25 in our hospital): (1) Fever (≥38.5°C), (2) splenomegaly, (3) Cytopenia (at least one cytopenia) (Hb < 90 g/L [neonatal <100 g/L], platelet <100  ×  109/L, absolute neutrophil count [ANC] < 1.0 × 109/L), (4) hypertriglyceridemia (fasting triglycerides ≥2.0 mmol/L) and/or hypofibrinemia (fibrinogen ≤1.5 g/L), (5) hemophagocytosis (bone marrow, spleen, lymph nodes, or liver), (6) ferritin ≥500 µg/L, (7) low/absent natural killer (NK) - cell activity (<10 solute units), (8) soluble CD25 (sIL-2r) (≥2,400 U/ml, or average >±2SD).

Data collection

Data was collected from medical records by two trained investigators. We collected demographic characteristics, laboratory metrics at admission, genetic testing data from patients, and treatment strategies during hospitalization. Common complications including multiple organ dysfunction syndrome (MODS), respiratory failure, heart failure, acute liver failure, acute kidney injury (AKI), acute respiratory distress syndrome (ARDS), sepsis, coagulopathy, severe pneumonia, electrolyte abnormalities, hypoproteinemia, liver dysfunction, and hyperlactatemia were also collected. Among them, respiratory failure is defined as PaO₂ < 60 mm Hg in ambient air. Heart failure is defined in line with guidelines published by the European Society of Cardiology in 2016 (16). AKI is defined in line with the clinical practice guidelines published by KDIGO 2012 (Kidney Disease: Improving Global Outcomes): serum creatinine levels increased by a factor of 1.5 from baseline (17). Hypoproteinemia is defined as: serum albumin <30 g/L. Other complications are diagnosed according to guidelines and/or consensus.

Statistical analysis

For continuous variables, we expressed them with median and interquartile range (IQR) and used the Mann–Whitney U test for between-group comparisons. For categorical variables, absolute values and percentages were expressed, and Fisher's exact test was used for between-group comparisons. The investigation of 30-day mortality prediction in patients with HLH was initially conducted by univariate analysis between the two groups to identify all potential associated factors. After binary analysis, the independent variables with p-value less than 0.05 were selected and entered into the multivariable Logistic regression analysis model. Survival curves were estimated by the Kaplan-Meier method. The survival time of the two groups was compared using the log-rank test. All statistical analyses were performed using SPSS (IBM SPSS Statistics for Windows, Version 26.0; IBM corp., Armonk, NY). Statistical significance for all tests was two-tailed p < 0.05. There were no trials of any specific treatment or preventive intervention. This study was approved by the Ethics Committee of the West China Second Hospital of Sichuan University (approval number: 2022-127).

Results

A total of 164 patients passed the inclusion criteria. After an initial review, 54 patients were excluded, including 40 patients with repeat admissions, 7 patients with lack of primary data, 6 patients with a hospital stay of less than 24 h, and 1 patient not meeting the HLH-2004 diagnosis standard. The final analysis included 110 patients (Figure 1).

Figure 1

Clinical data comparison between survival and death groups

As shown in Table 1, the median age of patients at diagnosis was 44 months (IQR 23.0–100.5). Of these, 62 patients (56.4%) were male. Our records show no significant differences between the two groups in terms of gender, age, weight. Of the 110 patients, 109 were admitted to the ICU, 34 (30.9%) died within 30 days of admission, and the 30-day OS was 69% (76/110). 109 (99.1%) patients developed fever and 90 (81.8%) developed splenomegaly. Strikingly, all patients developed hyperferritinemia. In addition, 63 (57.3%) patients had EBV infection, but there was no statistical difference between the death group and the survival group within 30 days. By comparing the death vs. survival within 30 days of admission, we found statistically significant differences in neutrophil counts, serum sodium counts, and splenomegaly between the two groups (p = 0.019, p = 0.022, and p = 0.031, respectively). There were no differences in other laboratory parameters between groups.

Table 2 is the information about the complications of our patients. Among them, the most common complication was coagulation disorder, which was diagnosed in 86 (78.2%) patients. There were statistically significant differences in MODS, respiratory failure, heart failure, acute liver failure, AKI, electrolyte disturbance, hypoproteinemia, and hyperlactatemia between the two groups in terms of death and survival within 30 days of admission (p = 0.011, p = 0.001, p < 0.001, p = 0.009, p < 0.001, p = 0.012, p = 0.004 and p = 0.011, respectively). There were no statistically significant differences in ARDS, sepsis, pneumonia, and liver dysfunction between the two groups.

Gene mutations were found in only 14 of the 110 patients during hospitalization (Supplementary Table S1), including 8 (57.1%) with UNC13D mutation, 4 (28.6%) with STXBP2 mutation, and 2 (14.3%) with PRF1 mutation. Notably, in our cohort, there was no statistically significant difference in sHLH diagnosis rates between the group that died within 30 days of admission and the group that survived (p = 0.06). As can be seen from Table 3, most patients were treated with antibiotics [109 (99.1%)] and dexamethasone [91 (82.7%)]. Continuous renal replacement therapy (CRRT), plasma exchange (PE), etoposide, dexamethasone, and assisted ventilation were statistically different between groups of deaths and survivors within 30 days of admission (p < 0.001, p = 0.031, p = 0.007, p = 0.002 and p < 0.001, respectively). Among them, the dose of etoposide in the survival group was 130.00 mg/m² (IQR 116.17–145.60), the reported dose of liquid in the death group was 120.00 mg/m² (IQR 108.55–128.00), and there was no significant difference in the dose of etoposide between the two groups (p = 0.205). There were no differences between groups in other therapies.

Multivariable Logistic regression analysis is used for HLH

As shown in Table 4, multivariable logistic regression analysis was used to predict risk factors for death within 30 days of admission to HLH patients. These include neutrophil count, serum sodium, respiratory failure, heart failure, acute liver failure, AKI, splenomegaly, hypoproteinemia, hyperlactatemia, CRRT, PE, etoposide, dexamethasone, and assisted ventilation. Multivariable logistic regression models do not include electrolyte disturbances because they are strongly related to electrolyte disturbances. At the same time, the model does not include MODS, because it is strongly associated with complications of organ injury such as respiratory failure and heart failure.

The results of multivariable logistic regression analysis showed that heart failure (OR = 13.389, 95% CI, 1.671–107.256, p = 0.015) and hypoproteinemia (OR = 4.841, 95% CI, 1.282–18.288, p = 0.020) were significantly associated with the increase in early HLH deaths. Other neutrophil counts, serum sodium, respiratory failure, AKI, acute liver failure, splenomegaly, hyperlactatemia, CRRT, PE, etoposide, dexamethasone, and assisted ventilation were not significantly associated with increased early mortality (p = 0.655, p = 0.176, p = 0.573, p = 0.210, p = 0.614, p = 0.157, p = 0.598, p = 0.775, p = 0.153, p = 0.719, p = 0.158, and p = 0.068, respectively). Based on the risk factors (heart failure and hypoproteinemia) derived from a multivariable logistic regression model, we plotted the 30-day survival curves of patients with HLH after admission (Figure 2). In addition, we performed subgroup analyses by respiratory failure (yes vs. no), heart failure (yes vs. no), acute liver failure (yes vs. no), AKI (yes vs. no), splenomegaly (yes vs. no), hypoproteinemia (yes vs. no), hyperlactic acidemia (yes vs. no), CRRT (yes vs. no), PE (yes vs. no), etoposide (yes vs. no), dexamethasone (yes vs. no), and assisted ventilation (yes vs. no), as shown in Figure 3, and we came to the same conclusion. Heart failure (OR = 12.636, 95% CI, 1.574–101.464, p = 0.017) and hypoproteinemia (OR = 5.125, 95% CI, 1.429–18.387, p = 0.012) were significantly associated with an increase in early HLH deaths.

Figure 2

Figure 3

Discussion

In our study, early mortality in HLH children remained high. In order to describe HLH patients who died shortly after hospitalization and to identify risk factors associated with early death, we described and analyzed the demographic information and clinical features of 110 children with HLH. The 30-day OS for children with HLH was 69.1%. The established risk factors for 30-day mortality were heart failure and hypoproteinemia, of which heart failure was strong risk factors (p < 0.05 and OR > 10).

In our patients, 30-day OS was 69.1%. We identified several other large case families of pediatric HLH patients in Chinese populations with a 30-day OS of 66%–86% (18–21). Our 30-day OS is similar to these results. Reports of early mortality in HLH patients in other regions are equally striking. Studies suggest that in one HLH patient with hypofibrinogenemia, the OS was 73.7% at 28 days (22). Strikingly, up to 109 (99.1%) of our case cohorts were admitted to the ICU, meaning that without timely and effective treatment, patients would be life-threatening. Therefore, it will be of great significance to analyze the risk factors for early death of patients with HLH, stratify the patients, and adopt timely and effective treatment strategies in a targeted manner. In addition, although previous reports suggest improved long-term survival in patients with HLH (14), it is still not optimistic. Therefore, this cohort follow-up of ours is continuing in order to characterize and analyze the long-term survival rates of HLH patients at 2 and 5 years.

A common complication was coagulation disorders, up to 86 cases (78.2%). In addition, because both sepsis and HLH are hyper-inflammatory syndromes and there is an overlap in diagnostic criteria between the two (23, 24), up to 66 (60%) of children are diagnosed with sepsis. This is consistent with previous research. It is difficult to explain the relationship between sepsis and HLH, and more sophisticated diagnostic indicators may be needed in the future to distinguish between the two diseases. Notably, there was no statistically significant difference in the incidence of sepsis between the two groups that died within 30 days of our and those who survived.

Our investigation identified clinical features that are significantly associated with early mortality, which is consistent with previous findings (25). Heart failure and hypoproteinemia are risk factors for increased mortality within 30 days. Because HLH can rapidly develop into MODS, if it is not treated in a timely and effective manner, it will endanger the lives of patients (26). Our study is consistent with a higher incidence of MODS (38.2% vs. 14.5%) in patients who died within 30 days than in surviving patients. However, because MODS is strongly associated with a variety of diseases such as heart failure, respiratory failure, and AKI, our final logistic regression model did not include MODS. Nonetheless, MODS remains a risk factor for death in patients with HLH (27), and targeted treatment is needed to improve patient outcomes.

The incidence of EBV-HLH is high in East Asian countries (28). The vast majority of our cohort is sHLH, and 57.3% of patients are positive for EBV infection Table 1, and other causes of sHLH are tumors, autoimmune diseases, or immunodeficiency. Our study shows that EBV infection has no difference between the survival and death groups and is not a risk factor for early death, which is similar to the previous conclusion that patients with EBV-HLH tend to have good outcomes when treated in a timely manner (29). In addition, in our cohort, sHLH did not affect the outcome of early treatment (p = 0.06) (Table 1). The therapeutic strategy for HLH is to support treatment and control of the overactivated immune system. It mainly includes immunosuppressive therapy (dexamethasone) and etoposide, as well as supportive therapy for complications. In our cohort, patients were treated with HLH-2004 therapy. Among them, 82.7% of patients were treated with dexamethasone, 50% were treated with etoposide, and 30% were treated with cyclosporine. It is worth noting that the rate of etoposide use in the death group is lower than that in the survival group (p = 0.007), which is related to the more serious complications (acute liver injury, acute renal failure) in the death group, because these complications are contraindications to etoposide use. Due to the different rates of etoposide use in the two groups, we calculated the dose of etoposide in both groups. In our cohort, etoposide was used once a week for the first 8 weeks at a dose of 128.28 mg/m² (IQR 114.55–145.22), and the dose was flexibly adjusted according to the patient's liver and kidney function. There was no significant difference in etoposide use between the survival group and the death group within 30 days of admission (p = 0.205), and the dose was not low. In the future, clinicians may need to find an optimal balance between contraindications to etoposide use and effective treatment in patients with HLH. By multivariate regression analysis, we found that the use of etoposide was not associated with a better outcome (OR). In addition, due to other complications, we gave patients CRRT (to rescue AKI), PE [to replace of cytokines and correct of coagulation disorders by fresh frozen plasma (FFP)], intravenous Immunoglobulin (to improve immunity), antibiotics (to fight infection), and assisted ventilation (to rescue respiratory failure).

The strength of this study is that it includes a large number of patients with rare diseases and describes in detail the demographic characteristics, clinical features, complications and treatment strategies of the patients. Our study provides a reference for identifying and stratifying high-risk patients for HLH and further targeted treatment strategies. Our study has some flaws, starting with the fact that this study is a single-center, retrospective study that may have selection bias and missing information and affect patient features and clinical data. Second, the vast majority of patients in our cohort are sHLH, so the findings of the study may be more appropriate for patients with sHLH. In addition, soluble CD25 is not routinely tested and may be missed diagnoses. Therefore, more accurate description and analysis of multicenter, prospective, randomized controlled studies with larger sample sizes are needed in the future.

Conclusions

We identified risk factors that are strongly associated with early mortality in pediatric patients with HLH. This may help clinicians stratify HLH patients and develop targeted treatment strategies. More research is needed to explore the best treatment strategies for patients with HLH to reduce early mortality in patients with HLH.

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