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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Phys. | doi: 10.3389/fphy.2019.00192

Calibration of Spectral Imaging Devices with Oxygenation-Controlled Phantoms: Introducing a Simple Gel-based Hemoglobin Model

 V. N. Du Le1*,  Myla Manser2, Sunny Gurm3, Ben Wagner4, Joseph Hayward2 and  Qiyin Fang2
  • 1University of California, Davis, United States
  • 2McMaster University, Canada
  • 3Other, Canada
  • 4Christie (Canada), Canada

An optical phantom model with stable oxygen saturation (StO2) and optical properties (OPs) is the key for quick and accurate calibration of spectral imaging devices. Toward this goal, we have developed gel-based phantoms using Agarose, polystyrene microsphere, purified hemoglobin and yeast. Theoretical values of OPs were calculated by applying Beer Lambert law and Mie theory whereas a robust fitting algorithm was used to extract StO2. Stability of OPs and StO2 in the phantoms was validated experimentally over a period of two weeks and the effect of pre-heating at 65o C degrees on StO2 was observed. Multi-spectral imaging (750, 780, 850 and 945 nm) of the phantoms were also performed on the FDA-approved commercial system to demonstrate the utility of the phantoms.

Keywords: Multispectral imaging (MSI), Hemoglobin, Optical properties, oxygen saturation, optical spectroscopy, Absorption

Received: 31 Jul 2019; Accepted: 04 Nov 2019.

Copyright: © 2019 Le, Manser, Gurm, Wagner, Hayward and Fang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. V. N. Du Le, University of California, Davis, Davis, United States,