Frontiers in Bioengineering and Biotechnology

Original Research

Published on 28 Apr 2025

A versatile genetic toolkit for engineering Wickerhamomyces ciferrii for tetraacetyl phytosphingosine production

in Synthetic Biology

Seong-Rae Lee
Jun Su Kang
Pyung Cheon Lee

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1586218

505 views

Original Research

Accepted on 25 Apr 2025

Deletion of succinic semialdehyde dehydrogenase Sad and chromosomal expression of phosphoenolpyruvate carboxylase as metabolic requirements for improved production of 2,4-dihydroxybutyric acid via malyl-P pathway using E. coli

in Synthetic Biology

T.A. Stefanie Nguyen
Ceren ALKIM
Nadine Ihle
Thomas Walther
Cláudio J.R. Frazão

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1589489

122 views

Original Research

Published on 25 Apr 2025

In situ intein-mediated multiprotein assembly via engineered cross-species consortia

in Synthetic Biology

Hao Wang
Jiajia Kang
Hui Gao

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1529655

433 views

Review

Published on 24 Apr 2025

Expanding the application of tyrosine: engineering microbes for the production of tyrosine and its derivatives

in Synthetic Biology

Jian Shen
Pengfu Liu
Bin Zhang
Bangce Ye
Shunqing Xu
Weike Su
Xiaohe Chu

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1519764

603 views

Brief Research Report

Published on 09 Apr 2025

Developing a media formulation to sustain ex vivo chloroplast function

in Synthetic Biology

Mariam Mohagheghi
Ali Navid
Thomas Mossington
Congwang Ye
Matthew A. Coleman
Steven Hoang-Phou

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1560200

629 views

Original Research

Published on 02 Apr 2025

Evaluation of the Salmonella type 3 secretion system (T3SS) as part of a protein production platform for space biology applications

in Synthetic Biology

Min-Kyoung Kang
James Bevington
Danielle Tullman-Ercek

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1567596

909 views

Original Research

Published on 31 Mar 2025

Analysis of the intrinsic value of life in the context of synthetic biology

in Synthetic Biology

Yi Zhang
Yuling Chen
Bohua Liao

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1536403

673 views

Original Research

Published on 27 Mar 2025

Determination of the genome-scale metabolic network of Bartonella quintana str. Toulouse to optimize growth for its use as chassis for synthetic biology

in Synthetic Biology

Emilio Garrote-Sánchez
Andrés Moya
Rosario Gil

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1527084

732 views

Methods

Published on 25 Mar 2025

An innovative high-throughput genome releaser for rapid and efficient PCR screening

in Synthetic Biology

Guoliang Yuan
Aljon Salalila
Sungjoo Hwang
Zhiqun Daniel Deng
Shuang Deng

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1547909

631 views

Brief Research Report

Published on 21 Mar 2025

Inducible Mtld expression facilitated the introduction of the mannitol synthesis pathway in Synechococcus elongatus PCC 7942

in Synthetic Biology

Jiahui Sun
Jinyu Cui
Xuejing Xu
Jinhui Tang
Huili Sun
Xiangxiao Liu
Xiangyi Yuan
Guodong Luan
Xuefeng Lu

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1575266

597 views

Review

Published on 11 Mar 2025

Advancements in metabolic engineering: unlocking the potential of key organic acids for sustainable industrial applications

in Synthetic Biology

Tengfei Wang
Han Xue
Hongling Liu
Haibo Yuan
Di Huang
Yi Jiang

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1556516

803 views

Original Research

Published on 28 Feb 2025

Dysregulated biosynthesis and hydrolysis of cyclic-di-adenosine monophosphate impedes sporulation and butanol and acetone production in Clostridium beijerinckii NCIMB 8052

in Synthetic Biology

Marian M. Awaga-Cromwell
Santosh Kumar
Hieu M. Truong
Eric Agyeman-Duah
Christopher C. Okonkwo
Victor C. Ujor

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1547226

945 views

Mini Review

Published on 27 Feb 2025

Cyanobacterial type I CRISPR-Cas systems: distribution, mechanisms, and genome editing applications

in Synthetic Biology

Yongjiu Zhang
Shuxiao Yang
Xianliang Zheng
Xiaoming Tan

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1552030

879 views

Review

Published on 24 Feb 2025

There and turn back again: the application of phage serine integrases in eukaryotic systems

in Synthetic Biology

Thais Torquato Sales
Marco Antônio de Oliveira
Lilian Hasegawa Florentino
Rayane Nunes Lima
Elibio Rech

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1478413

1,089 views

Original Research

Published on 20 Feb 2025

Cofactor engineering for improved production of 2,4-dihydroxybutyric acid via the synthetic homoserine pathway

in Synthetic Biology

Nadine Ihle
Laura Grüßner
Ceren Alkim
T. A. Stefanie Nguyen
Thomas Walther
Cláudio J. R. Frazão

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1504785

1,224 views

Editorial

Published on 04 Feb 2025

Editorial: Recent advancements in RNA technologies, diagnostics, and therapeutics

in Synthetic Biology

Jaimie Marie Stewart
Yaroslava G. Yingling
Kirill A. Afonin
Florent Hubé
Naoyuki Kataoka

Frontiers in Bioengineering and Biotechnology

doi 10.3389/fbioe.2025.1550225

1,107 views