Abstract
Introduction:
This study explores potential application of silver nanoparticles (AgNPs) to treat periodontal infection using Azadirachta indica leaf extract. The eco-friendly green synthesis process uses Azadirachta indica as a natural stabilizer and reducer, allowing AgNPs to be formed.
Methods:
Experimental AgNPs were characterized through transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), Zeta potential, ultraviolet-visible spectroscopy (UV-Vis) etc. The antimicrobial, antioxidant potential of AgNPs was tested to identify its efficacy against periodontal infections.
Results and discussion:
AgNPs were found spherical, nanosized (86 nm), with negative surface charge (−26.9 mV). TEM study depicted clear formation of discrete nanosize particles with smooth surface texture. Results showed strong antibacterial and anti-oxidant action of experimental AgNPs, preventing biofilm growth and bacterial viability. A higher binding affinity was observed between Quercetin and the selected protein, which is implicated in bacterial growth and biofilm formation on teeth. The study suggests that Azadirachta indica derived AgNPs could be a safe, efficacious, and eco-friendly alternative in place of conventional therapies to treat periodontal infection. Future in vivo studies are however warranted.
1 Introduction
Periodontal disease is a global issue affecting millions, with 10%–15% of adults experiencing severe periodontitis. It causes tooth loss, pain, and reduced quality of life and is linked to health disorders (). The financial impact is substantial, with medical treatment, tooth extraction, and reduced work efficiency (). Periodontal disease is primarily caused by the buildup of dental plaque, a viscous biofilm of bacteria on teeth and gumline (). Key pathogens include diverse range of parasites including Treponema denticola, Prevotella intermedia, Fusobacterium nucleatum, Eikenella corrodens, Porphyromonas gingivalis, causing inflammation and tissue damage (; ). Modern periodontal treatments have made significant progress in managing periodontal disease, but they still face challenges such as insufficient effectiveness in advanced cases, limited access to care, patient adherence, and the possibility of disease return (; ). The increasing incidence of dental implants has led to concerns about peri-implantitis, a disorder that resembles periodontitis but affects dental implants (Xu et al., 2022; ). Antibiotic resistance has also been wide spread incidence owing to irrational use of antibiotics in periodontal infections (; ).
Azadirachta indica, a plant largely popular for its unique flavonoids content and long history of use in traditional medicine system, has also been found to be effective in treating periodontal disease (). Flavonoids content of the Azadirachta indica leaf extract have antibacterial, antifungal, and anti-inflammatory properties, preventing the development of infections and reducing inflammation (; Singh and Mijakovic, 2022a). They also possess antioxidant properties, protecting gum tissues from oxidative stress. Azadirachta indica extracts can also enhance tissue regeneration and wound healing and can be used in oral hygiene practices ().
Nanoparticulate mediated delivery has been emerged as hopeful technologies in improving pharmacological effectiveness and also in modulating pharmacokinetic and pharmacodynamic properties of conventional therapeutics (Vijayaram et al., 2024). Silver nanoparticles (AgNPs) are being explored as a potential treatment for periodontal disease thanks to their strong antibacterial properties and ability to infiltrate bacterial biofilms. AgNPs have been found to reduce inflammatory responses, improve periodontal tissue regeneration (). Few reports have documented effective antimicrobial potency of AgNPs against periodontal infections (). A recent study by Hedayatipanah et al. (2024), reported green synthesis of AgNPs from propolis and its antimicrobial activity on the on the Porphyromonas gingivalis biofilm. Similarly, in another study, AgNPs conjugated with chlorhexidine or metronidazole demonstrated preferential antibacterial and anti-inflammatory potency in vitro ().
The work aims to explore the use of Azadirachta indica leaf extract as a reducing and stabilizing agent for producing AgNPs for periodontal therapy. Primary objective was to create a reliable method for generating AgNPs with controlled dimensions, morphology, and surface properties. To achieve the highest yield, stability, and antibacterial activity, the synthesis parameters were optimized. Experimental AgNPs were then examined using techniques like UV-Vis spectroscopy, FTIR, size analysis, zeta potential and TEM (). The antimicrobial activity of AgNPs against common periodontal pathogens was assessed using standard microbiological tests and antioxidant potential was evaluated using DPPH assay (). Additionally, docking and ADME analysis was performed to unveil the mechanism of interaction with key periodontal bacterial protein.
2 Materials and methods
2.1 Materials
The green leaves of Azardircata indica were gathered from the centurion university campus, odisha. The silver nitrate used in this study was acquired from Mahavir Chemical Supply, located in Bhubaneswar, Odisha.
Nutrient agar media, nutrient broth, and antibiotic assay media serve as standard substrates for antimicrobial studies. The free radical scavenging activity of the nanoparticles was assessed using the DPPH assay, employing methanol or ethanol as solvents and ascorbic acid obtained from Himedia, Mumbai, India. All the chemicals were of analytical grade.
2.2 Methods
2.2.1 Preparation of Azadirachta indica extract
The process of preparing an aqueous extract from Azadirachta indica leaves involved collecting the leaves and allowing them to dry completely in the shade. Once dried, the leaves were powdered and stored in an airtight container (; ). To make the extract, 5 gm of Azadirachta indica powder was boiled with 50 mL of distilled water for 30 min in a beaker. After cooling it to room temperature, the liquid extract was filtered and was stored at a temperature for future use.
2.2.2 Green synthesis of experimental silver nanoparticles
Green synthesis of AgNPs was carried out as per the method reported elsewhere. Briefly, a solution of silver nitrate was prepared by dissolving 10 mg of silver nitrate in 50 mL of double distilled water. The solution was heated to a temperature range of 50°C–60°C, and Azadirachta indica extract was slowly added. The solution was thereafter incubated at a temperature of 80°C for duration of 60 min until an identifiable change in color was seen. The presence of pale-yellow color in the solution suggested the creation of AgNps (; ). This was further confirmed by the visible absorbance peak at 438 nm.
2.2.3 Characterization of experimental silver nanoparticles
2.2.3.1 UV–visible spectroscopy
The UV-visible absorption spectra were used to determine the optimal time and temperature conditions for the reduction of silver ions by the colloidal mixture of prepared AgNPs and the substrate mixture of the plant extract. This spectroscopic technique allowed for precise control over the synthesis process, ensuring maximum efficiency in the production of silver nanoparticles (Wade, 2021; ).
2.2.3.2 Zetapotential and particle size
The zetasizer equipment was used to assess the particle size range and polydispersity of the nanoparticles. The particle size was determined by analyzing the temporal fluctuations in the scattering of laser light while the particles were undergoing Brownian motion (; ). This tool allows for the analysis of the average size of the particles in the sample. Overall, these analyses provide valuable insights into the characteristics and behavior of the nanoparticles in the colloidal solution ().
2.2.3.3 Fourier transmission infrared spectroscopy (FTIR)
The FTIR spectroscopy was used to identify the functional groups and their interactions in the sample. The resultant peaks corresponding to specific chemical bonds provide valuable information about the composition of the reaction mixture. The resultant AgNPs was scanned over a wave length of 4,000 cm−1 to 600 cm−1 in an FTIR instrument (SHIMADZU IR Prestige-21, Mumbai, India).
2.2.3.4 Morphological examination
The morphological examination allowed for the observation of the size, shape, and surface features of the AgNPs using the Transmission electron microscopy (TEM) and Selected Area electron diffraction (SAED). The analysis provides high-resolution images of the AgNPs, allowing for a detailed examination of their surface morphology (; ). Additionally, SAED analysis helps to determine the crystalline nature of the nanoparticles by analyzing the diffraction patterns produced when electrons interact with the sample ().
2.2.3.5 SAED analysis
The Tecnai G2 20 S-TWIN instrument was utilized to conduct measurements of selected area electron diffraction pattern. These measurements allowed for a detailed analysis of the crystal structure and orientation of the silver nanoparticles. This information is crucial in understanding the physical properties of the AgNPs ().
2.2.3.6 TEM analysis
TEM imaging allows for direct observation of the nanoparticles, enabling precise determination of their size and morphology. It is a powerful imaging technique that allows for high-resolution visualization of nanoscale structures, such as AgNPs (). TEM provides detailed information about the size, shape, and distribution of nanoparticles within a sample (Sharma et al., 2022). The specimens were prepared by applying a droplet of suspension with an approximate thickness of 60 nm onto a carbon membrane. The carbon membrane was then transferred onto a copper grid and allowed to dry before being inserted into the TEM ().
2.2.3.7 Antimicrobial activity
Treponema denticola and Porphyromonas gingivalis, commonest pathogens associated with periodontal diseases are selected for the study (). These are known to be highly resistant to conventional antibiotics (). Briefly, the agar plates were inoculated with the bacterial strains of Staphylococcus. aureus (ATCC 25923) P. gingivalis (ATCC 33277), T. denticola (ATCC 35405) and incubated at an optimal temperature for growth (). A chlorhexidine suspension at an equivalent concentration was introduced into another sterile disc as a standard. The growth inhibition zones around each disc were measured after incubation. The nutrient broth medium provided essential nutrients for the bacteria to grow and multiply, allowing for an accurate assessment of their susceptibility to the AgNPs (). 1 mg/mL solution of AgNPs was prepared using Milli Q water and subjected to appropriate sonication. Overnight grown culture in Luria-Bertani broth of the mentioned microorganisms was taken and diluted to an optical density of 1. A 500 μL volume of diluted bacterial suspension was evenly distributed across three distinct Luria-Bertani agar plates, each containing a different microorganism. Following the dispersion, two wells were created on each plate utilizing a well borer with an approximate diameter of 10 mm. Each of the three plates was filled with solutions of AgNPs at concentrations of 20 μg/mL, 50 μg/mL, and 75 μg/mL, and incubated for 24 h at 37°C. The antibacterial activity of AgNPs was assessed by measuring the presence of a clear zone of inhibition (ZOI) and from the minimum inhibitory concentration (MIC) (; ).
2.2.3.8 Antioxidant study
The DPPH (2-Phenyl-1-Picrylhydrazyl) technique with relatively small modifications was used to assess the antioxidant activity of the biosynthesized AgNPs. DPPH provides free radical with a prominent purple color which gets decolorized in presence of antioxidant. This approach is a dependable way to assess the radical scavenging activity of a molecule (; ).
Briefly, five distinct concentrations of biosynthesized AgNPs were prepared, with concentrations ranging from 0.5, 1, 1.5, 2, and 2.5 mg/mL. Each concentration was kept in a separate volumetric flask to which about 3 mL of a 0.1 mM methanolic solution containing DPPH radical was added. The solution was then rapidly agitated and left undisturbed for 30 min in a dark place at ambient temperature. The control sample included all the reaction reagents except for the AgNPs. Methanol was used for the purpose of baseline correction. Subsequently, the spectrophotometer was used to measure the absorbance at a wavelength of 517 nm. The findings were quantified as the percentage of radical scavenging activity, with ascorbic acid as a standard antioxidant.
The antioxidant activity data were quantified and reported as IC50values produced by linear regression analysis which is the minimum concentration (in micrograms) of the test sample needed to neutralize or block 50% of the radicals of DPPH concentration. Lower the IC50 value, the higher the antioxidant activity of the test sample.
The DPPH scavenging activity, expressed as a percentage of inhibition, is calculated using the formula:
2.2.3.9 Molecular docking
To depict the molecular interaction in between quercetin with virulence factor of selected periodontal causing bacteria’s i.e., P. gingivalis and T. denticola, in silico docking analysis was employed. The molecular structure of the selected compound was constructed utilizing canonical smiles from the PubChem database PubChem CID: 5280343. The PDB structure of virulence factor of P. gingivalis and T. denticola was acquired from the RCSB Protein Data Bank (PDB id: 5Y1A and 3R15 respectively). Following that, the receptor and ligand structures were uploaded to the Argus Lab App for docking. The Argus Lab App outcome was modified for the optimal structure and visualized with Discovery studio visualizer 2.5. Subsequently, the relationship was verified by evaluation of the obtained binding energy, including ACE (atomic contact energy), global energy and attractive (vdw van der Waals). The proteins employed for the analysis included collagenase, protease, and urinase, which are responsible for catalyzing specific biochemical reactions in the oral cavity (; ).
2.2.3.10 Assessment of ADMET
The Swiss ADME offers a practical alternative to the traditional approach of designing drugs from natural materials or synthetic compounds. The Swiss ADME approach was used to assess the pharmacokinetics, bioavailability, drug-likeness, and medicinal chemistry compatibility of the quercetin in order to enhance its properties and determining the bioavailability and efficacy of quercetin as a potential drug candidate.
3 Results and discussion
The main goal is to prepare nanomaterial-based therapies that are effective, non-hazardous, and compatible with the human body, potentially improving treatment outcomes and patient care in periodontal dentistry (Wasilewska et al., 2023; Singh and Mijakovic, 2022b).
In the current study, we have used Azadirachta indica leaf extracts to show the production of AgNPs. The impact of different experimental conditions on the biogenesis of silver nanoparticles was also investigated. In addition, silver nanoparticles that were produced under the most favorable circumstances were analyzed using several aspects of analytical equipment. The antibacterial and antioxidant activities of the produced silver nanoparticles were assessed. The results demonstrated that the silver nanoparticles exhibited strong antibacterial properties against a variety of pathogens. Furthermore, the antioxidant activity of the nanoparticles was found to be significant, indicating potential applications in biomedical and environmental fields (; Sharifi-Rad et al., 2024).
3.1 Green synthesis of silver nanoparticle from leaf extract of Azadirachta indica
The formation of silver nanoparticles was confirmed by surface plasmon resonance activity with a peak of 429 nm which indicated the reduction of AgNO3 and the subsequent formation of silver nanoparticles were validated, all achieved without the use of any hazardous substances (; ). It can be determined that this is an ecofriendly friendly technique commonly referred to as green synthesis of silver nanoparticles using the Azadirachta indica extract as shown in Figure 1.
FIGURE 1
3.2 Characterization of optimized silver-nanoparticle
The characterization includes UV analysis, zetapotential, FTIR, SAED, and TEM. These techniques are used to determine the size, shape, and stability of the silver nanoparticles. UV analysis is used to measure the absorption spectrum of the nanoparticles, while zeta potential measures the surface charge. FTIR is used to analyze the chemical composition of nanoparticles, and SAED and TEM are used to visualize the crystal structure and morphology of the nanoparticles. Overall, these characterization techniques provide a comprehensive understanding of the optimized silver nanoparticles (Yun et al., 2022; Zhang et al., 2022a).
3.3 UV-visible spectroscopy
The UV-vis absorption spectra showed a peak at 438 nm, indicating the formation of silver nanoparticles. As shown in Figure 2. The yellow color of the reaction mixture confirmed the successful reduction of Ag+ ions to AgNPs (). Analysis of the UV-vis spectra showed that the peak intensity at 438 nm increased with longer incubation time. This suggests that there were more AgNPs in the solution (). Additionally, the temperature had a significant effect on the reaction rate, with higher temperatures leading to faster synthesis of AgNPs. The stability and production of silver nanoparticles in water are tested using UV-Vis spectroscopy. Surface plasmon activity is detected during the formation of nanoparticles, as shown in Figure 2.
FIGURE 2
3.4 FTIR
The FTIR value of AgNPS suggested that the nanoparticles have a strong absorption peak at around 400 cm−1, indicating the presence of silver-metal bonding. Additionally, the FTIR spectrum shows peaks at 1,348.96 cm−1 and 3,400 cm−1, suggesting the presence of carbonyl groups and hydroxyl groups on the surface of the nanoparticles. Overall, the FTIR analysis indicates that the AgNPS are well-formed and have functional groups that could potentially be useful for various applications in nanotechnology (Shirmohammadi et al., 2023; ). The FTIR characterization image of AgNPs has been shown in Figure 3.
FIGURE 3
3.5 Zetapotential and particle size
The zeta potential of optimized AgNPs was found to be −26.9 mV, and the particle size was 86 nm with a polydisopersibility index of about 0.6832. This result suggests that the AgNPs would stable in solution due to their high negative zeta potential, indicating repulsion between particles. The relatively small particle size suggests good dispersion and the potential for enhanced reactivity in various applications. The low polydispersibility index also indicated a relatively uniform size distribution, further supporting the stability and potential effectiveness of the AgNPs (; Singh et al., 2020). As shown in Figures 4A, B.
FIGURE 4
3.6 SAED
The results showed that the AgNPs had a uniform size distribution and exhibited a face-centered cubic [FCC] crystal structure. The Selected area electron diffraction (SAED) data obtained from the optimized AgNPs exhibits a circular ring that exhibits the crystalline characteristics of the silver nanoparticles. The dimensions and morphology of the nanoparticles play a crucial role, since their functionalities are contingent upon their size and form. This suggests that the optimized AgNPs were well-formed and possess high purity Figures 5.
FIGURE 5
3.7 TEM
This analysis determined the diameters ranging from 1 to 100 nm, revealed the existence of evenly distributed and highly crystalline silver nanoparticles. Most of them exhibited a roughly spherical morphology. The image showed that the periphery of the produced silver nanoparticles seemed to be less dense compared to the central region. Biomolecules function as capping agents, inhibiting the aggregation of nanoparticles (; ). As shown in Figure 6.
FIGURE 6
3.8 Antimicrobial activity
An antimicrobial study of silver nanoparticles showed promising results against a wide range of pathogenic bacteria and a standard drug to compare the effectiveness of AgNPs which shows a greater zone of inhibition against P. gingivalish, measuring 24 mm, T. denticola, measuring 22 mm; and S. aureus, measuring 20 mm, at a concentration of 75 μg/mL as shown in Table 1 (; ). This research demonstrates that both Azadirachta indica and silver nanoparticles possess potent antibacterial abilities that successfully fight periodontal disorders (Yang et al., 2024; Zhang et al., 2022b). The large inhibition zone values showed that AgNPs could work well as an antibacterial agent against bacteria as shown in Figure 7.
TABLE 1
| Name of organism | Diameter of zone of inhibition [mm] | |||||
|---|---|---|---|---|---|---|
| AgNPs (25 μg/mL) | Chlorhexidine (25 μg/mL) | AgNPs (50 μg/mL) | Chlorhexidine (50 μg/mL) | AgNPs (75 μg/mL) | Chlorhexidine (75 μg/mL) | |
| S. aureus | 15 ± 1.125 | 18 ± 2.052 | 18 ± 1.154 | 22 ± 1.456 | 24 ± 1.259 | 25 ± 1.961 |
| T. denticola | 14 ± 2.28 | 18 ± 1.32 | 17 ± 1.46 | 20 ± 2.45 | 26 ± 1.38 | 24 ± 1.34 |
| P. gingivalis | 18 ± 1.81 | 23 ± 1.61 | 22 ± 2.63 | 25 ± 1.95 | 25 ± 2.58 | 28 ± 2.67 |
In vitro antimicrobial efficacy evaluation of AgNPs on selected periodontal pathogens, viz., S. aureus, T. denticola and P. gingivalis through zone of inhibition assay.
Data are expressed Mean ± SD.
FIGURE 7
3.9 Antioxidant activity
An Examination of the antioxidant properties of optimized silver nanoparticles (AgNPs) produced from Azadirachta indica demonstrated promising findings for possible medicinal uses. When 2.5 mg/mL of aqueous extract was added, the enzyme activity was inhibited by 45.11%. However, using AgNPs resulted in a substantially greater inhibition rate of 62%. By contrast, ascorbic acid, a well-recognized antioxidant, attained an inhibition rate of 72.48%. The results suggest that AgNPs have a higher antioxidant capacity compared to the aqueous extract. The IC50 value, representing the concentration needed to block 50% of enzyme activity, was lower for AgNPs compared to the aqueous extract. This indicates that AgNPs have a greater ability to remove harmful free radicals and protect cells from oxidative stress. The study’s findings highlight the strong ability of the AgNPs to scavenge DPPH free radicals, hence strengthening their potential for use in the treatment of many disorders associated with oxidative stress. As shown in Table 2. The study’s findings highlight the strong ability of the AgNPs to scavenge DPPH free radicals, strengthening their potential for use in periodontal treatment as shown in Figure 8.
TABLE 2
| Concentration mg/mL | %Inhibition (aqueous extract) | %Inhibition AgNPs | %Inhibition (Ascorbic acid) |
|---|---|---|---|
| 0.5 | 19.27 ± 1.2 | 40.29 ± 2.4 | 51.78 ± 1.7 |
| 1 | 22.89 ± 2.3 | 45.98 ± 2.7 | 59.89 ± 1.3 |
| 1.5 | 28.49 ± 1.6 | 50.68 ± 1.4 | 64.28 ± 1.1 |
| 2 | 35.05 ± 2.1 | 56.89 ± 1.5 | 67.99 ± 1.4 |
| 2.5 | 45.11 ± 1.5 | 62.01 ± 1.9 | 72.48 ± 1.8 |
In vitro anti-oxidant activity analysis of experimental AgNPs vs. aqueous extract through DPPH free radical scavenging assay; Ascorbic acid taken as standard.
FIGURE 8
3.10 Molecular docking result
The presentation of the primary phytochemical was examined using in silico molecular docking simulation (Quercetin) on different virulence factor of selected periodontal causing bacteria’s. The findings demonstrated that quercetin interacted with distinct amino acids of the alkyl group at the active site of virulence factor of selected periodontal causing bacteria. These visualizations help to understand quercetin’s molecular interactions with enzymes, revealing its therapeutic potential. The docking algorithm was able to predict the Quercetin ligand pose with those mediators viz5Y1A and 3R15 binding energy score of-9.37151 kcal/mol and −9.04139 kcal/mol and amino acids in Collagenase, Protease, and Urease, resulting in binding energies of −7.3, −8.7, and −8.6 kcal/mol, respectively. The results indicate that a large negative score of the mediators viz5Y1A binding energy score of −9.37151 kcal/mol strongly binds with quercetin rather than other proteins (Wu et al., 2024; ). In Figure 9A, the two-dimensional pictures illustrate how quercetin binds to specific sites on the enzymes, affecting their activity. The 3D visualizations in Figure 9B provide a more detailed look at the complex molecular interactions between quercetin and the enzymes, highlighting the specific structural changes that occur during the binding process ().
FIGURE 9
3.11 In-silico Swiss-ADME analysis
The ADMET attribute of quercetin encompasses several characteristics, including physicochemical properties, lipophilicity, water solubility, pharmacokinetics, and drug-likeness. These attributes play a crucial role in determining the bioavailability and efficacy of quercetin as a potential drug candidate ().
As per Lipinski’s rule, the molecular mass is always less than 500 Da. This rule helps in predicting the likelihood of a compound being orally bioavailable. As per the result, quercetin has a molecular mass of 302.24 Da, which indicates it is a good conductor for the process. The number of H-bond acceptors is below 10, which is 7, which follows Lipinski rule 5. The molar reactivity is about 78.03, which is in the range of 40–130. This suggests that the compound has a moderate reactivity level that indicate a balance between the desired activity of the drug and its potential safety profile, making it potentially suitable for further drug development. The topological surface area can predict the solubility parameter of a compound, which is about (TPSA) 131.36 Å2. This value suggesting moderate absorption potential and the compound is unlikely to cross the BBB effectively, meaning it may not have central nervous system (CNS) effects. A TPSA under 140 Å2 is generally favorable for oral bioavailability, so this result suggests that the compound suitable for oral administration. The log P value, which is less than 5, is what determines a compound’s lipophilicity, and the average value is 1.22. The water solubility property of the compound shows that it is soluble in nature. The pharmacokinetic property shows high GI absorption; there is no blood-brain barrier permeation. The compound can be metabolized by the enzymes cytochrome P1A22, P2D6, P2D6, and P3A. The skin permeability log Kp is about 7.05 cm/s suggests that the compound has very high permeability through the skin. This is crucial for topical or transdermal drug delivery, as it indicates that the drug can effectively pass through the skin barrier and enter systemic circulation. Moderate reactivity and high skin permeability suggest that the compound could be effective in skin-based delivery methods.
The pharmacokinetics property shows the different parameters for designing a drug molecule and using the polymer to get more action. The drug-likeness property of the compound shows there is no violation of Lipinski, and the bioavailability score is about 0.55. A bioavailability score of 0.55 indicates that 55% of the orally administered compound reaches systemic circulation. This suggests that the compound has moderate oral absorption but may still be affected by factors like first-pass metabolism or poor permeability. The ADMET property of Quercetin was illustrated in the Table 3.
TABLE 3
| Physicochemical properties | Lipophilicity | Water solubility | Pharmaco-kinetics | Drug likeness | |||||
|---|---|---|---|---|---|---|---|---|---|
| Formula | C15H10O7 | Log Po/w (iLOGP) | 1.63 | Log S (ESOL) | −3.16 | GI absorption | High | Lipinski | Yes; 0 violation |
| Molecular weight | 302.24 g/mol | Log Po/w (XLOGP3) | 1.54 | Solubility | 2.11e-01 mg/mL; 6.98e-04 mol/L | BBB permeation | No | Ghose | Yes |
| Num. heavy atoms | 22 | Log Po/w (WLOGP) | 1.99 | Class | Soluble | P-gp substrate | No | Veber | Yes |
| Num. arom. heavy atoms | 16 | Log Po/w (MLOGP) | −0.56 | Log S (Ali) | −3.91 | CYP1A2 inhibitor | Yes | Egan | Yes |
| Fraction Csp3 | 0.00 | Log Po/w (SILICOS-IT) | 1.54 | Solubility | 3.74e-02 mg/mL; 1.24e-04 mol/L | CYP2C19 inhibitor | No | Muegge | Yes |
| Num. rotatable bonds | 1 | Consensus Log Po/w | 1.23 | Class | Soluble | CYP2C9 inhibitor | No | Bioavailability Score | 0.55 |
| Num. H-bond acceptors | 7 | Log S (SILICOS-IT) | −3.24 | CYP2D6 inhibitor | Yes | ||||
| Num. H-bond donors | 5 | Solubility | 1.73e-01 mg/mL; 5.73e-04 mol/L | CYP3A4 inhibitor | Yes | ||||
| Molar Refractivity | 78.03 | Class | Soluble | Log Kp (skin permeation) | −7.05 cm/s | ||||
| TPSA | 131.36 Å2 | ||||||||
Insight into ADMET property analysis of Quercetin using SWISS ADMET.
The BOILED-Egg model simplifies the calculation of molecular polarity and lipophilicity. According to the findings of the BOILED-Egg model, the molecules would appear in the white section of the egg, which would indicate that they were absorbed by the gastrointestinal tract. The BOILED-Egg model provides a visual representation of where the molecule is likely to be absorbed in the body, guiding further research and development efforts. Overall, the compound appears to have favorable properties for drug development, with high solubility, absorption, and metabolism rates. The skin permeability and pharmacokinetics data suggest that the compound could be effective when administered orally or through the skin. Additionally, the lack of blood-brain barrier permeation indicates that the compound may have a lower risk of causing central nervous system side effects. The adherence to Lipinski’s rule of five and favorable bioavailability score further support the potential of this compound as a drug candidate [69]. The BOILED-Egg model of Quercetin was illustrated (Figure 10).
FIGURE 10
4 Conclusion
One easy, green, and inexpensive way to make AgNPs is to use the extract from the leaves of the Azadirachta indica plant. The Azadirachta indica leaves contain phytochemicals, including flavonoid (Quercetin), which function as both reducing and capping agents throughout the synthesis process. The silver nanoparticles (AgNPs) show strong antibacterial efficacy against pathogenic gram-negative strains. The ability of AgNPs to break down bacterial cell membranes, stop metabolic processes, and make reactive oxygen species makes them antibacterial. The Azadirachta indica derived AgNPs not only possess antibacterial activities but also exhibit substantial antioxidant activity. The Azadirachta indica leaves include phytochemicals that enhance the ability of the AgNPs to neutralize free radicals and reduce them. Their size was determined to be 86 nm, with a potential of −26.9 mV. Furthermore, the SAED, FTIR, AFM, and TEM analyses demonstrated advantageous morphological properties of the silver nanoparticles. The promising results from in silico docking and ADME analyses demonstrated that Quercetin exhibits strong binding affinity to the target protein, which is critically involved in bacterial growth and biofilm formation on teeth. Additionally, ADME analysis indicated high skin permeability, supporting the potential of a synergistic combination of Quercetin and silver nanoparticles for effective topical application in periodontal therapy. The detailed study of the synthesized AgNPs gives us important information about their structure and function, which supports the idea that they could be used to treat gum disease. The study indicates that the synthesized AgNPs have acceptable pharmacokinetic features and low toxicity, making them appropriate for biomedical applications, particularly periodontal usage.
5 Future scope
Anticipating the future, the potential of this study is quite promising. Continued improvement of the synthesis process and optimization approaches may result in the powerful formulations that have improved medicinal benefits. Furthermore, exploring the possible synergistic impacts with other therapeutic agents might open up novel approaches for periodontal disorders. Additionally, continued research into the effectiveness and safety of these novel treatments will be crucial in advancing the field of periodontics.
Statements
Data availability statement
The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding authors.
Author contributions
BB: Software, Writing–review and editing. BS: Data curation, Methodology, Writing–original draft. GP: Investigation, Writing–original draft. DB: Data curation, Formal Analysis, Writing–original draft. KS: Conceptualization, Validation, Writing–original draft.
Funding
The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. The work was funded by Ajman University, United Arab Emirates. Authors express their gratitude for the financial support.
Acknowledgments
I, KPS would thankful to the Deanship of graduate studies and research, Ajman University for necessary support towards Article Publication charges for this manuscript.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Supplementary material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fchem.2024.1489253/full#supplementary-material
Abbreviations
AgNPs, Silver nanoparticle; FTIR, Fourier transmission infrared spectroscopy; TEM, Transmission electron microscopy; DPPH, 2-Phenyl-1-Picrylhydrazyl; SAED, Selected area electron diffraction, MIC, Minimum Inhibitory Concentration; TPSA, Topological surface area.
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Summary
Keywords
silver nanoparticles, Azadirachta indica, antimicrobial, antioxidant, molecular docking, ADMET
Citation
Barik B, Satapathy BS, Pattnaik G, Bhavrao DV and Shetty KP (2024) Sustainable synthesis of silver nanoparticles from Azadirachta indica: antimicrobial, antioxidant and in silico analysis for periodontal treatment. Front. Chem. 12:1489253. doi: 10.3389/fchem.2024.1489253
Received
31 August 2024
Accepted
27 September 2024
Published
15 October 2024
Volume
12 - 2024
Edited by
Afzal Basha Shaik, Jawaharlal Nehru Technological University, Kakinada, India
Reviewed by
Mithun Rudrapal, Vignan’s Foundation for Science, Technology and Research, India
Adil Shareef Mohammed, Temple University, United States
Updates
Copyright
© 2024 Barik, Satapathy, Pattnaik, Bhavrao and Shetty.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Gurudutta Pattnaik, gurudutta.pattnaik@cutm.ac.in; Krishna Prasad Shetty, kprasad11@gmail.com
Disclaimer
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