Guan Guoqiang1Qu Liang2Zhao Yani1Wang Pengyun1Kong Fanzhuo1Zhang Yuyang1Lin Zhiyuan1Ni Xing1Zhang Xue1Lu Qiongya1
Zou Bin1*- 1School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China
- 2School of Food and Bioengineering, Wuhu Institute of Technology, Wuhu, China
Glucose oxidase (GOx), as a molecular recognition element of glucose biosensors, has high sensitivity and selectivity advantages. As a type of biosensor, the glucose oxidase electrode exhibits advantages such as ease of operation, high sensitivity, and strong specificity, promising broad application prospects in biomedical science, the food industry, and other fields. In recent years, with the advancement of nanotechnology, research efforts to enhance the performance of GOx biosensors have primarily focused on improving the conductive properties and specific surface area of nanomaterials, while neglecting the potential to modify the structure of the core component, GOx itself, to improve biosensor performance. Rapid modification of the GOx surface through chemical modification techniques yields a new modified enzyme (mGOx). Meanwhile, composite techniques involving carbon nanomaterials can be employed to further enhance sensor performance. This article reviews the construction methods and optimization strategies of glucose oxidase electrodes in recent years, along with research progress in their application in electrochemical sensing for glucose detection, and provides an outlook for future developments.
1 Necessity of glucose testing
With the rapid development of the social economy, people’s dietary structure has also changed, and carbohydrate-rich diets have become the norm and are increasing day by day. According to the International Diabetes Federation (IDF) in 2019, 463 million people worldwide are currently living with diabetes. China ranks first in the number of diabetic patients, with about 116.4 million people, 25% of the world’s share. The large diabetic community has attracted more and more social attention. People are starting to consciously avoid carbohydrate-rich foods, so foods with low-calorie or non-nutritive sweeteners and sugar-free products have become very popular. According to the GB28050-2011 National Food Safety Standard “General Principles for Nutrition Labeling of Prepackaged Foods”, “sugar-free or sugar-free” specifically refers to a sugar content of no more than 0.5 g per 100 g or 100 mL of solid or liquid food. The complex diversity of food samples makes it necessary to develop a highly sensitive, low-cost, and rapid glucose detection method. To help people understand the sugar content in food and to achieve the purpose of disease prevention (Zhang Y. et al., 2018; Wang et al., 2024). So far, a variety of glucose detection methods have been developed, including fluorescence, optical, sonic, thermal, electrochemical, and colorimetric methods have been used for the detection of glucose due to their simple operation, however, the sensitivity of this method is often poor and insufficient to quantify glucose in the sample, and to circumvent these problems, electrochemical methods have been extensively studied (Li et al., 2021; Ma et al., 2022; Sun et al., 2016). Although different glucose detection techniques have been reported, electrochemical sensors due to their high sensitivity, low cost, and simple instrumentation, it is still considered the most successful analytical tool for glucose detection (Han et al., 2021; Ma et al., 2021; Hu et al., 2024). Glucose electrochemical sensing detection has the advantages of high detection sensitivity, accuracy and rapidity, and low construction cost, which can not only detect human blood glucose levels but also can be used to detect trace amounts of glucose in food, medicine, and biological samples (Bai et al., 2020; Peng et al., 2019), Therefore, electrochemical sensing analysis occupies an important position in glucose detection. Meanwhile, in recent years, the improvement of sensor performance has become a research hotspot, especially in terms of sensitivity, selectivity and long-term stability. For example, Tong et al. (Tong et al., 2024) prepared nanocomposites (PGOx@M-Xene/CS) by efficient electrostatic assembly of GOx polygels (PGOx) onto MXene nanosheets, PGOx can enhance the stability of the enzyme, while MXene’s extensive large specific surface area reduces its influence on enzyme stability. The constructed glucose sensor had a linear range of 0.03–16.5 mM, with a sensitivity of 48.98 μA mM-1·cm-2, and the limit of detection was 3.1 μM. The current remained at 85.83% of the initial current value after 200 cycles. Ramachandran et al. (Ramachandran et al., 2022) effectively prepared hexagonal CoMn2O4 electrode material by simple hydrothermal technique using KOH as surfactant and the obtained sensor maintained 85% capacitance after 4,000 consecutive charge/discharge cycles with 81% maximum column efficiency.
2 Advances in electrochemical detection sensors for glucose
2.1 Glucose oxidase-based electrodes
The first enzyme electrodes and enzyme sensors were described by Clark and Updike in the 1960s (Hovancová et al., 2017). In their concept, glucose oxidase (GOx) could be trapped in a semi-dialysis membrane on an oxygen electrode, and glucose concentration could be determined indirectly by monitoring oxygen consumption. Five years later, Updike and Hicks proposed an innovative method for the preparation of glucose analysis enzyme electrodes, which was designed to achieve rapid and accurate determination of glucose concentration in the blood by firmly embedding glucose oxidase (GOx) in a polyacrylamide gel layer overlying an oxygen electrode (Wang P. et al., 2019). Since then, electrochemical glucose sensors have developed rapidly, and electrochemical enzyme electrode technology has become the main method for detecting glucose (Xia et al., 2022). Today, electrochemical glucose sensors have undergone four transformations in principle, forming four generations of sensors, the principle of which is shown in Figure (Figure 1).
Figure 1. Schematic diagram of electron transfer of fourth-generation electrochemical glucose sensor.
2.1.1 First-generation glucose biosensors
The first generation of glucose enzyme electrochemical sensors utilized molecular oxygen as an electron acceptor, and the electrochemical signal was transmitted by measuring the decreasing oxygen concentration or the released H2O2, but the detection method was greatly affected by dissolved oxygen, and the detection potential was too high, which made the detection results easily affected by other interfering substances (Wen et al., 2020; Bu et al., 2024). On the other hand, the H2O2 produced during the reaction accumulated, and the concentration increased, resulting in the loss of glucose oxidase activity (Qin et al., 2017).
In the 80s of the 20th century, a large number of studies focused on solving the problems of oxygen interference and redox interference (Zhang et al., 2019). There are many solutions to oxygen interference. Gough et al. (Gough et al., 1985) overcame oxygen interference by increasing oxygen/glucose permeability with the help of a mass migration limiting membrane. Wang et al. addressed oxygen restriction in glucose biosensors by using oxygen-rich Carbon Paste Electrodes (Wang and Lu, 2009). D’Costa et al. avoided oxygen demand deficiency by replacing GOx with glucose dehydrogenase, which does not require oxygen cofactors, and the solution to redox interference was mainly to reduce interference to the electrode surface by selective coating (D Costa et al., 1986). Zhang et al. found that a cellulose acetic-Nafion composite membrane can effectively eliminate electrochemical interference such as acetaminophen (Zhang et al., 1994). Millilista et al. immobilized GOx using an electrochemically synthesized polyphenylenediamine (PPD) membrane that selectively removed ascorbic acid interference (Malitesta et al., 1990; Rong et al., 2019).
2.1.2 Second-generation glucose biosensors
The second-generation biosensor overcame the limitations encountered by the first-generation biosensor. They used redox mediators instead of oxygen to transfer electrons from the enzyme to the surface of the working electrode. The resulting reducing medium is further oxidized on the electrode formation of oxidation mediators, thus the ampere signal was detected. Various organic/inorganic chemicals are used as electronic media, mainly ferrocene derivatives (Monkrathok et al., 2024), ferricyanide (Nikitina et al., 2023), quinones (Mtemeri and Hickey, 2023), transition metal complexes (Wijayanti et al., 2023), and phenothiazine (Teymourian et al., 2020). Campbell et al. realized the detection of glucose by covalently coupling glucose oxidase to a redox medium containing ferrocene, combined with intramolecular electron transfer and electron self-exchange (Campbell et al., 2017). Zhou et al. achieved the detection of glucose by incorporating ferrocene (FC) as an electronic medium and immobilizing glucose oxidase (Gox) on the gate electrode, and tests with simulated blood samples were performed, and the modified sensor showed a bilinear response in the range of 0.6–26.3 mM, whereas conventional sensors (e.g., PEDOT-based) have a narrower linear range (0.5 μM-0.1 mM) (Zhou et al., 2024). Donini et al. (Donini et al., 2020) fixed glucose oxidase to redox graphene and used it for the detection of glucose. Lin et al. (2019) used hydrophilic and positively charged alpha-poly-L-lysine (alpha PLL) as an embedded substrate to fix negatively charged glucose oxidase (GOx) and ferric cyanide (FIC) onto SPCE to construct a disposable second-generation glucose biosensor, and tested in real human serum samples, the sensitivity was significantly improved (from 117.4 to 212.1 nA/mM mm2), and the linear range and detection limit were also superior to the pre-improved sensor. The emergence of redox mediators solved the problem of the dependence of glucose sensors on oxygen, but the sensors still had limitations. The presence of redox agents increased the cost of sensors, and many redox agents were biotoxic, limiting the range of sensor applications. Therefore, it is hoped that a way to replace or eliminate redox mediators while maximizing the current intensity of the sensor (Ahmad et al., 2023). Therefore, in the third generation of electrochemical glucose sensors, the biocatalyst was directly bound to the electrode surface where the signal was converted and amplified, thus ensuring that the electron transfer between the biocatalyst and the contact surface of the conductive carrier was as efficient and fast as possible (Khan et al., 1996; Ravikumar et al., 2021).
2.1.3 Third-generation glucose biosensors
The third-generation glucose biosensor is not dependent on oxygen and redox mediators, and the goal is to transfer electrons directly from the redox site of GOx to the electrode in the absence of a medium. However, the redox center of GOx, flavin adenine dinucleotide (FAD), is deeply embedded in the three-dimensional macrostructure of the enzyme molecule, which acts as an inherent barrier to direct electron transfer between GOx and the electrode. A range of nanomaterials, such as conductive polymers, metal oxides, carbon nanotubes, and graphene, have been used as electrode materials for modified electrodes (Xu et al., 2017; Ma et al., 2019; Shen et al., 2019; Shah et al., 2024). The distance between the active center of the enzyme and the surface of the electrode is effectively reduced to create a suitable environment and promote the smooth progress of the direct electron transfer process between the enzyme and the electrode. Rafighi et al. (2016) synthesized a glucose biosensor using a hybrid of graphene and polyethyleneimine-gold nanoparticles as enzyme carriers, and tested in actual blood samples, the sensitivity of the sensor reached 9.3 μA·mM-1cm-2, superior to carbon quantum dots reported in the literature (6.1 μA mM-1). Tao et al. (Ming et al., 2024; Zhu et al., 2022)modified the sensor by electrochemically depositing gold nanoparticles to enhance its conductivity for better detection of glucose, tested in clinical serum samples, the improved sensor showed only a 13.1% decrease in sensitivity after 7 days in bovine serum compared to a 45.1% decrease in the uncoated version, demonstrating that the PU film significantly extends the service life. Chu et al. (2015) successfully prepared composite carriers by in situ electrodeposition of gold nanocubes on a thiol graphene film. These were uniformly distributed on the electrode surface and had a regular nanostructure. This glucose biosensor exhibits high sensitivity and selectivity, and increased sensitivity to 221.0 μA mM-1·cm-2, 4-7 times higher than similar sensors in the literature, and retained 79.3% sensitivity after 2 weeks of storage (Figure 2). However, the structure of the enzyme protein may change after fixation on the surface of the electrode, which will affect the enzyme activity and the stability of the electrode (Apaliya et al., 2017; Wang L. et al., 2019; Jing et al., 2020). Therefore, preliminary research on third-generation EBGS has focused on how to achieve DET, which mainly involves the following aspects: 1) adding an electron transfer domain to the enzyme itself, 2) modifying with conductive nanomaterials, and 3) modifying with other materials (Song et al., 2024).
Figure 2. The preparation scheme of Au nanocube/graphene composite film based biosensor. Due to the existence of -SH on graphene surface, oxidase can directly adhere on the film by the interaction produced by the -S-S- from protein and -SH from grapheneReproduced with permission from ref, (Chu et al., 2015). Copyright 2015, Elsevier Publication.
Compared with the first and second generations of glucose biosensors, the third generation of glucose biosensors has achieved better results. However, its core principle is still controversial, and whether the DET that generates electrical signals is real is still doubtful. The redox peak may not come from the electron transfer of active enzymes but because the enzyme’s structure is destroyed, resulting in FAD exposure. Moreover, external environmental factors such as temperature, pH, and humidity may affect their dependence on enzyme activity. In addition, the performance of biosensors also depends on the thickness of the enzyme layer, which is high, resulting in signal attenuation or loss (Ou et al., 2021; Yin et al., 2023; Xu et al., 2016).
2.2 Glucose dehydrogenase-based electrodes
Glucose dehydrogenase (GDH), an NAD(P)+-independent oxidoreductase, has attracted a lot of attention in recent years for its application in glucose sensors. Different types of GDH play an important role in improving the sensitivity, selectivity, and stability of the sensor. Glucose dehydrogenase forms complexes with cofactors such as flavin adenine dinucleotide (FAD), nicotinamide adenine dinucleotide (NAD), or pyrroloquinoline quinone (PQQ) (Zhao et al., 2021). For example, the glucose sensor developed by Kim et al., which involves binding the enzyme to the anionic self-assembled monolayer on the electrode through electrostatic interactions, exhibits high sensitivity. (Kim et al., 2012) (Figure 3). Chen et al. (2022) developed an NAD (+)-dependent dehydrogenase/NPG/SPE biosensing platform for the electrochemical detection of glucose by modifying a screen-printed electrode (SPE) with NPG and NAD (+)-dependent dehydrogenase.
Figure 3. Schematic for binding of PQQ-GDH onto the SAMs of 11-MUA: (a) electrostatic and (b) covalent binding via EDC/NHS chemistry. Reproduced with permission from ref, (Kim et al., 2012). Copyright 2015, Springer Science Publication.
3 Non-enzymatic glucose sensor
Non-enzymatic glucose sensors are a technology that does not rely on an enzyme-catalyzed reaction to detect glucose concentration. The core principle of the non-enzymatic glucose sensor lies in the precise detection of glucose concentration through an electrochemical catalytic reaction. The detection system relies on the intrinsic catalytic activity of metal nanomaterials to realize the direct oxidation process of glucose molecules on the electrode surface in an alkaline medium. The catalytic unit is usually constructed with highly active metal nanoparticles, including two systems of noble metals (Au, Ag, Pt) and transition metals (Ni, Co, Cu) (Li H. et al., 2024). These nanostructures significantly enhance the charge transfer efficiency and catalytic site density of the sensor by increasing the effective reaction interface and optimizing the mass transfer channels. Although non-enzymatic sensors circumvent the problem of enzyme activity decay, their selectivity, environmental adaptability and long-term stability are still the main bottlenecks (Table.1). Therefore, glucose oxidase-based sensors are still in the main position for glucose detection.
Table 1. Comparison of enzyme versus non-enzyme sensors.
The fourth-generation glucose biosensor is a non-enzymatic glucose sensor that uses an artificial substance with enzyme-like catalytic properties to replace glucose oxidase and oxidize glucose directly on the surface of the electrode. Fourth-generation glucose sensors (FGGS) are designed to enhance glucose-sensing technology and reduce the number of intermediate stages equired for glucose measurement. Diagnostic efficiency and cost-effectiveness can be improved by using these sensors, which are fabricated using electrocatalytic copper nanostructures (Naikoo et al., 2022; Kilic et al., 2023). Ahmad et al. fabricated an electrochemical-based non-enzymatic glucose biosensor using engineered layered CuO nanoleaves, which shows high sensitivity (1467.32 μA/(mM cm2)), linear range (0.005–5.89 mM), and detection limit of 12 nM (S/N = 3) (Ahmad et al., 2021). The fourth generation has the advantage of using chemically derived materials, which is more suitable for large-scale production, and the chemically produced identification parts can exert better uniformity and reproducibility compared to enzymes prepared by biotechnology. On the other hand, this type of device can be affected (Pohanka, 2021). Therefore, the use of glucose oxidase electric sensors for glucose detection is still the mainstream.
4 Enhancement of glucose oxidase activity stability
In the development of glucose biosensors, the enhancement of glucose oxidase activity stability is the core technical difficulty. In recent years, the linkage strategies for Gox activity attenuation have focused on the following directions: nanomaterial encapsulation protection: encapsulating GOx by metal-organic frameworks (MOFs) or mesoporous silica to limit the enzyme molecular conformational changes. For example, Mao et al. (Mao et al., 2025) successfully synthesized glucose oxidase (GOx)@Zn-HHTP, which significantly improved the stability of the encapsulated GOx and was applied to construct an ECL glucose sensor with 8.5-fold increase in sensitivity and 25-fold decrease in detection limit, which was successfully applied to detect glucose in sweat. Covalent cross-linking enhancement: Glutaraldehyde or genetically engineered bifunctional cross-linkers (e.g., SpyCatcher/SpyTag) were used to enhance the enzyme-electrode interface binding. For example, Chmayssem et al. (2023) used a chitosan-based hydrogel to capture glucose oxidase (GOx) and crosslinked the entire substrate with glutaraldehyde, and the resulting biosensor was able to maintain its stability over 6 months of storage. Biomimetic polymer coatings: polydopamine (PDA) or amphoteric ionic polymers (e.g., poly (sulfobetaine)) were utilized to form antifouling coatings. For example, Chen et al. (2023) combined the good hydrophilicity and biocompatibility of PDA with the high loading properties and peroxidase-like activity of HKUST-1 to synthesize the PDA/HKUST-1/MWCNTs/GOx biosensor, which was used for glucose detection with a sensitivity of 178 μA mM-1cm-2, a linear range of 0.005 mM, and a limit of detection of 0.12 μ M. The initial current response value remained at 82.0% after 30 days (Figure 4).
Figure 4. Copper-based metal–organic frameworks (MOF) and multi-walled carbon nanotubes (HKUST-1-MWCNTs) composite were synthesized by one-step hydrothermal method, and PDA-enzyme-HKUST-1-MWCNTs composite was prepared by one-pot method for the construction of glucose biosensors. Reproduced with permission from ref, (Chen et al., 2023). Copyright 2022, Springer Science Publication.
5 Chemical modification of glucose oxidase
5.1 Introduction to glucose oxidase
In the presence of molecular oxygen, glucose oxidase catalyzes the oxidation of β-D-glucose to D-glucono-delta-lactone and hydrogen peroxide. The resulting D-glucono-delta-lactone is sequentially hydrolyzed to D-gluconate by lactonase, and the resulting hydrogen peroxide is hydrolyzed by catalase to oxygen and water (Bauer et al., 2022). GOx has a relative molecular mass of 130 × 103–175 × 103 and exists as a glycosylated homodimer, with each subunit noncovalently bound to one flavin adenine dinucleotide (FAD) molecule, and the two subunits are bound to each other by forces such as salt bridges and hydrogen bonds (Huang et al., 2022). In the catalytic process, GOx uses molecular oxygen as an electron acceptor and FAD coenzyme as an electron carrier to catalyze specifically the formation of D-glucose-delta-lactone and hydrogen peroxide (H2O2) from β-D-glucose (Wang et al., 2022). GOx is most active under weakly acidic conditions, and the pH range in which activity is stable is 3.5–7.5; other than that too much acid and too much alkali will inactivate the enzyme molecule. GOx is available from a variety of sources. Bacterial, fungal, herbal, and animal sources are the main ones. Fungi are considered to be the richest source and are widely used for industrial applications, A. niger, and P. glaucoma were the first identified sources of GOx isolates, and glucose oxidase isolated from Aspergillus niger is considered to be the most stable (Khatami et al., 2022; He et al., 2022; Guo et al., 2021; Shang et al., 2019). Glucose oxidase has been widely used in biomedical applications (Min et al., 2023), bio-Fenton oxidation (Vaidyanathan et al., 2023), feed field (Liang et al., 2023), textile bleaching (Tzanov et al., 2002), reducing wine processing (Pickering et al., 1998), food packaging deaerator (Ge et al., 2012), etc.
5.2 Methods of chemical modification of enzymes
Chemical protein modification provides a large toolbox for the study and modification of enzymes, which play key roles in many important biological events in organisms; in particular, by associating desired properties/functions (affinity probes, fluorophores, reactive tags, etc.) with naturally or synthetically modified amino acid residues, chemical protein modification provides a useful way to identify enzyme locations and elucidate enzyme functions (Yu et al., 2022; He et al., 2018; Yan et al., 2024).
Chemical modifications of enzymes can be divided into covalent modifications, non-covalent modifications, targeted modifications, and macromolecular coupling (Table.2). The specific modification mechanisms are shown in the following table (Table.3). Surface modification is carried out using functionalized small molecules bearing alcohol, aldehyde, carboxylic acid, or isothiocyanate groups, and these reactions are carried out on the enzyme surface with exposed functional groups to form covalent bonds (Giri et al., 2021; Noro et al., 2022). Polymer coupling is the most common strategy for macromolecular modification enzymes. Among macromolecules such as polyglycol, polypropylene, and dextran, polydiethanolization is the most studied method, such as covalent polyglycol bonding with partial molecules or macromolecules (Noro et al., 2022; Li T. et al., 2024; Zhang et al., 2020; Chen et al., 2024). Chemical modifications of enzymes can alter affinity, specificity, or stability, while selective modifications enable the labeling of enzymes, allowing insight into complex biological processes, significant improvements have been made in the field of chemical modification/capture strategies for proteomic analysis, these methods emerged to be able to analyze the activity of enzymes in the body, and the chemicals used are often site-specific variants of those chemicals that are more commonly used to modify proteins and enzymes (Diaz-Rodriguez and Davis, 2011; Ren et al., 2018).
Table 2. Classification of chemical modifications of enzymes.
Table 3. Mechanistic explanation of chemical modification of enzymes.
5.3 Research progress on glucose oxidase modification
The specific recognition of glucose by glucose oxidase makes glucose oxidase biosensors the most common electrochemical biosensors for glucose detection. However, the current sources and types of glucose oxidase are limited, and rapid molecular modification of the enzyme, such as chemical modification techniques, is needed to alter its activity and selectivity. In recent years, the surface modification of glucose oxidase has attracted the attention of researchers (Table.4) (Liu et al., 2024). Back in 1991, Kunugi et al. (1992) introduced a ferrocenyl group on glucose oxidase by combining the carboxyl group on ferrocene acid with the amino group on glucose oxidase to form a modified enzyme, the modified enzyme can be detached from oxygen for electron transfer with the electrode. Halalipour et al. (2020) attached phenyl derivatives to the carboxyl side chain or amino side chain of glucose oxidase (Gox) and modified Gox with hydrophobic aniline and benzoate, respectively, which showed that aniline-modified Gox had the highest catalytic efficiency, followed by benzoate-modified Gox, and the natural Gox performed the worst. It is demonstrated that hydrophobic modification increases Gox activity and is more resistant to high temperatures, at 80°C and 240 MPa, the rate of inactivation of aniline-modified GOx was 3.7-fold lower than that of the natural enzyme and 2.8-fold lower than that of the benzoic acid-modified enzyme. Hosseinkhani et al. (Hosseinkhani et al., 2004; Shi et al., 2019; Jiang et al., 2020) used citric anhydride to modify the lysine residues of glucose oxidase chemically, the pH tolerance of the modified enzyme was enhanced, and UV and fluorescence spectra also indicated that the chemical modification resulted in more exposure to hydrophilic residues. In addition, Liu et al. (Liu et al., 2013) also utilized the carboxyl group on 1-pyrenebutyric acid to covalently bind to the amino group on the surface of glucose oxidase, so that the enzyme surface carries a pyrenyl group with a conjugated structure, which was then loaded on graphene and assembled into a glucose oxidase electrode, which greatly improved the detection range of glucose concentration, the linear detection range of the modified sensor was 0.2–40 mM with a detection limit of 0.154 mM (S/N = 3), which greatly improved the detection range of glucose concentration, and was tested in real human serum samples, which greatly improved the detection range of glucose concentration, with a 5.1% decrease in activity in the first week, and still retaining 82.2% activity after 4 weeks. In addition to the surface modifications of the Gox, Zappelli et al. (Zappelli et al., 1978; Hou et al., 2017) modified FAD with epoxy acid and subsequently coupled it with polyethyleneimine to form a macromolecular FAD, which showed a 12-fold increase in stability compared to unmodified FAD in a circulating system with alanine as substrate. Fornerod et al (2023) used porous aluminosilicates and silicates for surface modification of Gox loading, the glucose detection sensitivity of the amino-modified electrode was 0.26 μA/mM (0–14 mM), which was higher than that of the unmodified electrode of 0.16 μA/mM (0–8 mM), and the detection limit of the modified electrode was 1.4 mM lower than that of the unmodified electrode (3.6 mM) (Figure 5). Lv et al. (2021) modified Gox with amines to obtain enzymes with higher enzymatic activity and to give the sensor a higher peak current intensity, the electron transfer rate constant of the amine-modified sensor, 2.54 s-1, was 1.39 times higher than that of the unmodified sensor (1.83 s-1). It can be seen that chemical modification can quickly and inexpensively change the properties of glucose oxidase, and glucose oxidase will be more and more widely used.
Table 4. Chemical modification of glucose oxidase.
Figure 5. Poly (isoprene)-block-poly (ethylene oxide) (PI-b-PEO) micelles were co-assembled with aluminum silicate nanoparticles in solution and then spin-coated onto the working electrode to form a thin film. The hybrid coating was then calcined to condense the inorganic nanoparticles into a continuous matrix and remove the block copolymer (BCP) micelles, resulting in pores. Reproduced with permission from ref, (Fornerod et al., 2023). Copyright 2023, ACS Publication.
6 Multifunctional applications of carbon nanomaterials for glucose sensing
Since the birth of the first glucose oxidase electrode in 1962, people have been trying to find suitable materials to modify glucose oxidase on the electrode surface and maximize enzyme activity. Carbon nanomaterials have attracted great research interest due to their unique size, strength, electrical and surface area properties, and good biocompatibility and stability (Zeng et al., 2016). Therefore, carbon nanomaterials have become popular materials in the field of catalysis and sensing (Table 5). Currently people apply carbon nanomaterials to electrochemical sensors, which not only simplifies the size of the sensor, but improves the sensitivity as well as the stability of the electrode, but also greatly shortens the response time so that the electrochemical sensor is toward the direction of practical, miniaturization, and multifunctional development (Table 6) (Kaçar et al., 2014; Bi et al., 2018; Dong et al., 2024).
Table 5. Classification of carbon nanomaterials in glucose sensors.
Table 6. Mechanism of the effect of different carbon nanomaterials on sensor performance.
6.1 One-dimensional materials: carbon nanotubes and nanofibers
Carbon nanotubes are allotropes of carbon, which are part of the fullerene family of structures and are small in diameter (nanoscale) and length (micrometer) (Wang et al., 2021; Sridharan et al., 2022). Typical carbon nanotubes are tubular carbon atom systems composed of hexagonal carbon atoms, which have special properties due to their symmetrical structure. Their behavior depends entirely on their spiral nature, and because of this, they play the role of semiconductors or metals (Soni et al., 2020). Carbon nanotubes (CNTs) are highly regarded members of the synthetic carbon allotrope due to their unique arrangement of carbon atoms, sp2 hybridization, and cylindrical structure arranged between C-C distances of 1.42 Å and 3.4 Å layers, which make them different from other nanocarriers (Prajapati et al., 2022). According to their number of layers, they are divided into (a) single-walled carbon nanotubes (SWCNTs): graphite sheets of single-atom thickness bent into cylinders; (b) Multi-walled carbon nanotubes (MWCNTs): several layers of graphite sheets arranged concentrically (Bin et al., 2022). Single-walled carbon nanotubes (SWCNTs) have a simple chemical structure and clean surface properties, which give them a high degree of chemical stability. In contrast, multi-walled carbon nanotubes (MWCNTs) have a more complex wall layer, resulting in a complex and variable surface structure, which can adsorb and bind a large number of surface functional groups. This structural complexity provides significant advantages for MWCNTs in a wide range of applications (Han et al., 2024; Xia et al., 2023; Su et al., 2023) (Figure 6).
Figure 6. The role of carbon nanotubes in electrochemical sensors.
Carbon nanotubes were introduced by Sumio Iijima in 1991 (Sumio, 1991) and formally described and named, its unique tubular nanostructure (L/D ratio may be as high as 132 million:1), superior strength, and significant physicochemical properties quickly caught the attention of researchers (Upadhyay et al., 2011; Zeng et al., 2022). In addition, CNTs have excellent electrical conductivity, strong adsorption capacity, high sensitivity, good biocompatibility, and excellent chemical stability, making them ideal nanomaterials for the preparation of biosensors (Ma et al., 2019; Wang et al., 2021; Wang et al., 2009). Kyuhwan et al. discovered that carbon nanotubes (CNTs) as covalently immobilized materials for Gox can effectively maintain Gox based on activity and stability, the biosensor prepared based on this was used to detect glucose, and the sensitivity reached 53.5 μA·mM-1cm-2, which remained 86% active after 2 weeks, compared to the DTSSP modified gold electrode (0.026 s-1) and glassy carbon electrode (0.2 s-1), this electrode achieved an electron transfer rate of 1.14 s-1, a 5.7∼44-fold improvement (Hyun et al., 2015). Yin et al. (2024) used a glucose monitoring skin patch prepared from a hollow syringe modified with glucose oxidase (GOD) and carbon nanotubes (CNTs) as an electrochemical sensor for glucose monitoring and an integrated circuit for signal processing and transmission, and displaying real-time blood glucose levels on a smartphone via Bluetooth, which continuously measures glucose in real time in live animals with micromolar sensitivity and a lifetime of more than 14 days of useful life.
Carbon nanofibers (CNFs), with their excellent electrical conductivity and remarkable specific surface area, have attracted much attention in nanotechnology and materials science in recent years. Its excellent electron mobility ensures that electrons can be transferred at the electrode interfaces in an efficient and low-resistance manner, thus realizing an efficient electrical signal transduction mechanism. In addition, the nanoscale structure of CNFs provides many active sites, further facilitating rapid electron transfer and reaction kinetics. For example, Zhang et al. (2018a) modified GOx electrodes by combining manganese dioxide nanoparticles and carbon nanofiber nanocomposites and completed practical application validation by spiking samples of urine in order to obtain sensors with detection limits as low as 0.015 mM, recoveries in the range of 101.0%–104.8%, and retention of 71.9% of activity after 30 days, and the sensitivity of the MnO2- CNFs-modified sensor was 5.4 times more sensitive to H2O2 (33.1 μA/mM) than the MnO2-modified electrode only (6.1 μA/mM) (Figure 7).
Figure 7. (A) Schematic illustration for the synthesis of MnO2–CNFs nanocomposites. the MnO2–CNFs conjugates were prepared via the interaction of the carboxyl group with Mn2+ for subsequent on-spot chemical deposition of MnO2 onto CNFs. (B) Schematic representation of the mechanism of electrocatalysis of glucose catalyzed by glucose oxidase. Reproduced with permission from ref, (Zhang et al., 2018b). Copyright 2018, Springer Science Publication.
6.2 Two-dimensional materials: graphene, graphene oxide and graphyne
Following CNTs, scientists Geim and Novoselov prepared a new carbon nanomaterial, graphene (GR) in 2004 (Morales-Narvaez et al., 2017; Sun and Joshi, 2010; Wu et al., 2017). Graphene biosensors have been vigorously developed in the past decade due to their small size, unique conductive and optical properties (such as fluorescence quenching and conductivity), and good biocompatibility that meet the high efficiency and diversity requirements of biosensors (Zhu et al., 2017; Zhou et al., 2016; Zhao et al., 2022). Pakapongpan and Poo-Arporn, (2017); Wu et al. (2017) prepared a GOx biosensor by self-assembling glucose oxidase (GOx) on covalently modified magnetic nanoparticles (Fe3O4 NPs). The graphene material facilitated the electron transfer between the enzyme and the electrode surface, and the biosensor showed a fast amperometric response to glucose (3 s), a linear range from 0.05 to 1 mM, a low detection limit of 0.1 μM, significantly lower than that of GNs/ZnO/SPE (70 μM), and high sensitivity (5.9 μA mM-1), and the current response of the sensor remained 95.6% of the initial value after 1 month’s storage at 4°C. Wu et al. (2022) developed a microelectrode glucose biosensor based on 3D hybridized nanoporous platinum/graphene oxide nanostructures for rapid glucose detection in tomato and cucumber fruits, which achieved high glucose detection sensitivity (11.64 muA calibrated in glucose standard solution), low detection limit (13 mumol/L) and fast response time (95% steady-state response within 3 s). Liu et al. (2019) immobilized ferrous ions and glucose oxidase on GDY sheets and presented GDY-based composites with dual enzyme activity. Rat serum was used as a test sample and the electrodes obtained were superior to V2O5 nanowires (10–2000 µM) and Cu-Ag/GO composites (1–30 µM), with R2 = 0.998, and 0.89 µM for Fe-GDY/GOx, which is significantly lower than Fe/CeO2 NPs (3.41 µM) and H2TCPP/Fe2O3 NPs (2.54 µM) (Figure 8). Antonova et al. (2024) developed a soft microfluidic glucose sensor catalyzed and mediated by bimetallic palladium and platinum supported on reduced graphene oxide with 1,10-phenanthroline-5,6-dione. The sensor demonstrated a linear amperometric response to glucose within the range of 50–900 μM at an applied potential of 0.2 V, exhibiting a detection limit of 37 μM and a sensitivity of 30 μA cm-2 mM-1. The wearable sensor prototype enables convenient non-invasive measurement of exercise-induced sweat glucose levels for personalized diabetes monitoring.
Figure 8. Schematic representation of the mechanism for immobilizing ferrous ions and glucose oxidase on Graphdiyne. Reproduced with permission from ref, (Liu et al., 2019). Copyright 2018, ACS Publication.
6.3 Three-dimensional materials: hollow carbon spheres and carbon aerogels
Carbon aerogels (CAs) are mesoporous materials with abundant porosity and high specific surface area that are suitable for many practical applications. In addition, CAs have been found to have excellent biocompatibility due to their three-dimensional (3D) structural network, good electrical conductivity, exceptional chemical and environmental stability, and strong adhesion capabilities, which are ideal for the development of next-generation catalyst materials (Thirumalraj et al., 2018). Yu et al. (2015) reported a zirconium phosphate-carbon aerogel (ZrP-CA) composite material, where the ZrP-CA/GOx configuration exhibited a linear calibration range of 0.12–2.0 mM for glucose detection, surpassing conventional GOD/In2O3–chitosan (0.005–1.3 mM) and GOD–graphene–CdS (0.025–1.19 mM) systems. The sensitivity reached 5.56 μA mM-1cm-2, demonstrating a enhancement compared to traditional ZrP-based sensors (0.41 μA mM-1cm-2).
6.4 Zero-dimensional materials: carbon quantum dots and nanodiamonds
Carbon quantum dots (CDs) are a class of fluorescent carbon-based nanoparticles with a particle size of <10 nm, and the abundant oxygen-containing functional groups (-OH, -COOH) on their surfaces provide multiple anchor sites for Gox immobilization. Compared with conventional materials, the advantages of CDs are reflected in the excitation wavelength-dependent fluorescence property of CDs that can work synergistically with electrochemical signals, and the cytotoxicity of CDs (IC50 > 500 μg/mL) is significantly lower than that of CNTs (IC50 ≈ 50 μg/mL) for implantable sensors. Hu et al. (Hu et al., 2020) developed a sensitive fluorescent microfluidic sensor based on carbon quantum dots (CQDs), cadmium telluride quantum dots (CdTe QDs) aerogel, and glucose oxidase (GOx), with all experimental validations performed using human urine specimens for glucose detection. The sensor demonstrated exceptional storage stability, retaining stable fluorescence signal (R/G ratio) and colorimetric response after 30-day storage at −20°C. It achieved a broad glucose detection range from 0 to 13 mM with a detection limit of 0.223 mM (S/N = 3). Zhao et al. (2006) developed an electrochemically pretreated glucose biosensor based on non-doped nanocrystalline diamond (N-NCD)-modified gold electrodes for selective glucose detection, achieving a broad linear calibration range from 10 μM to 15 mM with a low detection limit of 5 μM (S/N = 3), which significantly outperforms conventional sensors. The biosensor retains 75% of its initial sensitivity after 30 days of storage.
We compare the effects of different carbon nanomaterials on glucose sensors (Table 7) and add the mechanisms by which they increase the electron transfer rate of the sensors (Table 8).
Table 7. Comparison of different carbon nanomaterials for glucose sensor applications.
Table 8. Effect of different carbon nanomaterials on the electron transfer rate of glucose sensors.
7 Synergistic chemical modification of carbon nanomaterials and enzymes with glucose sensors
The high conductivity of carbon nanomaterials provides a fast channel for electron transfer between the enzyme and the electrode; their large specific surface area also provides abundant sites for enzyme immobilization, while chemical modification can shorten the electron transfer path and further reduce the interfacial resistance; by regulating the enzyme microenvironment, conformational changes can be reduced, which improves the catalytic efficacy and thermal stability of the enzyme. The adsorption properties of carbon nanomaterials synergize with the selective screen of chemical modification, which can effectively shield interferences such as ascorbic acid and uric acid and improve the specificity of the sensor (Table 9).
Table 9. Comparison of typical cases of synergies.
8 Significance and challenges of glucose oxidase electrode modification
Glucose oxidase electrochemical sensors have attracted much attention because of their ability to detect glucose specifically, but maintaining the activity of the enzyme is also a challenge. By introducing materials such as carbon nanotubes, graphene, and metal nanoparticles with high specific surface area, good electrical conductivity, and catalytic activity on the electrode surface and by chemically modifying the enzyme, the efficiency of electron transfer between the enzyme and the electrode can be significantly improved, thereby increasing the sensitivity of the sensor. These modified materials not only provide more attachment sites for GOx, but also enhance the interaction between the enzyme and the electrode, making the oxidation of glucose more efficient. For sensor performance, future research should focus on optimizing sensor sensitivity, improving selectivity and interference immunity, and increasing long-term stability and commercialization potential.
In recent years, some enzyme-modified nanomaterial sensors have entered the commercialization stage, e.g., FreeStyle Libre (Abbott) has successfully occupied a significant position in the global market of glucose detection. However, the following key issues still need to be addressed in order to realize their large-scale production: reproducibility of material synthesis (e.g., the diameter and chirality control of single-walled carbon nanotubes (SWCNTs) still relies on a complex gas-phase deposition process, resulting in large performance variations between batches), compatibility of enzyme immobilization processes (existing enzyme modification technologies (e.g., covalent modification, macromolecular coupling) are susceptible to environmental fluctuations in continuous production), device miniaturization and integration (lab lab labs are susceptible to environmental fluctuations), and the use of enzyme-enabled devices.), miniaturization and integration of equipment (laboratory sensors rely on bulky electrochemical workstations, while commercial equipment requires integrated signal processing modules).
9 Outlook for future applications of the glucose oxidase electrode
Most of the current research is still based on standard solutions or simulated samples, and the validation of real blood samples needs to be further optimized for immunity (e.g., ascorbic acid, uric acid, etc.) and long-term stability. Future studies need to focus on the validation of sensor performance in real complex samples (e.g., whole blood, food extracts) and the development of portable devices in combination with miniaturization techniques to meet the needs of clinical diagnosis and immediate testing (POCT). At the same time, there are many issues to be faced to realize the utility of glucose oxidase electrodes as follows. Existing carbon nanomaterials (e.g., graphene, carbon nanotubes) may trigger an inflammatory response upon long-term skin contact, so encapsulating materials that are flexible, breathable, and biologically inert, such as polyurethane-nanofibrillar cellulose composite membranes, need to be developed. At the same time, surface functionalization techniques (e.g., PEG modification) are used to reduce the immunogenicity of the materials, thus further improving their biocompatibility. Second, wearable devices need to adapt to sweat pH fluctuations, mechanical deformation and temperature changes to ensure sensor stability in dynamic environments. Meanwhile, continuous glucose monitoring requires the integration of multiple sensors (e.g., pH, temperature compensation modules), but the energy consumption of nanomaterial devices limits miniaturization, so multimodal data synchronization needs to be optimized to balance energy consumption.
Author contributions
GG: Conceptualization, Funding acquisition, Writing – review and editing. QL: Writing – review and editing. ZYa: Writing – review and editing. WP: Writing – original draft. KF: Writing – review and editing. ZYu: Writing – review and editing. LZ: Writing – review and editing. NX: Writing – review and editing. ZX: Writing – review and editing. LQ: Writing – review and editing. ZB: Writing – review and editing.
Funding
The author(s) declare that financial support was received for the research and/or publication of this article. This work was financially supported by the Scientific Research Startup Funding Project for Introduced Talents of Wuhu Institute of Technology (No. wzyrc202403), the Wuhu Science and Technology Innovation Strategy Research Special Project (No. 2024swwzx07), the Wuhu Institute of Technology Food Nutrition and Health Innovation Team (No. 2023jxtd06), the “Double-Teacher and Dual-Ability” Teacher Team Construction Project of Anhui Provincial Department of Education (No. [2024]117).
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Generative AI statement
The author(s) declare that no Generative AI was used in the creation of this manuscript.
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References
Ahmad, N., Naeem, M., Ali, H., Alabbosh, K. F., Hussain, H., Khan, I., et al. (2023). From challenges to solutions: the impact of melatonin on abiotic stress synergies in horticultural plants via redox regulation and epigenetic signaling. Sci. Hortic. 321, 112369. doi:10.1016/j.scienta.2023.112369
Ahmad, R., Khan, M., Mishra, P., Jahan, N., Ahsan, M. A., Ahmad, I., et al. (2021). Engineered hierarchical CuO nanoleaves based electrochemical nonenzymatic biosensor for glucose detection. J. Electrochem. Soc. 168 (1), 017501. doi:10.1149/1945-7111/abd515
Antonova, I. V., Ivanov, A. I., Shavelkina, M. B., Poteryayev, D. A., Buzmakova, A. A., and Soots, R. A. (2024). Engineering of graphene-based composites with hexagonal boron nitride and PEDOT:PSS for sensing applications. Phys. Chem. Chem. Phys. 26 (9), 7844–7854. doi:10.1039/d3cp05953g
Apaliya, M. T., Zhang, H., Yang, Q., Zheng, X., Zhao, L., Kwaw, E., et al. (2017). Hanseniaspora uvarum enhanced with trehalose induced defense-related enzyme activities and relative genes expression levels against Aspergillus tubingensis in table grapes. Postharvest Biol. Technol. 132, 162–170. doi:10.1016/j.postharvbio.2017.06.008
Bai, J., Zhu, Y., Li, J., Zhang, Y., Dong, Y., and Xiao, X. (2020). Effects of bitter melon saponin on the glucose and lipid metabolism in HepG2 cell and C. elegans. J. Food Qual. 2020, 1–9. doi:10.1155/2020/8860356
Bauer, J. A., Zámocká, M., Majtán, J., and Bauerová-Hlinková, V. (2022). Glucose oxidase, an enzyme “ferrari”: its structure, function, production and properties in the light of various industrial and biotechnological applications. Biomolecules 12 (3), 472. doi:10.3390/biom12030472
Bi, J., Li, Y., Wang, H., Song, Y., Cong, S., Yu, C., et al. (2018). Presence and formation mechanism of foodborne carbonaceous nanostructures from roasted pike eel (Muraenesox cinereus). J. Agric. Food Chem. 66 (11), 2862–2869. doi:10.1021/acs.jafc.7b02303
Bin, Z., Feng, L., and Yan, Y. (2022). Biomimetic metalloporphyrin oxidase modified carbon nanotubes for highly sensitive and stable quantification of anti-oxidants tert-butylhydroquinone in plant oil. Food Chem., 388. doi:10.1016/j.foodchem.2022.132898
Bu, Q., Yu, F., Cai, J., Bai, J., Xu, J., Wang, H., et al. (2024). Preparation of sugarcane bagasse-derived Co/Ni/N/MPC nanocomposites and its application in H2O2 detection. Industrial Crops Prod. 211, 118218. doi:10.1016/j.indcrop.2024.118218
Campbell, A. S., Islam, M. F., and Russell, A. J. (2017). Intramolecular electron transfer through poly-ferrocenyl glucose oxidase conjugates to carbon electrodes: 1. Sensor sensitivity, selectivity and longevity. Electrochimica Acta 248, 578–584. doi:10.1016/j.electacta.2017.07.150
Chen, S. Y., Shang, K., Gao, X., and Wang, X. (2017). The development of NAD+-dependent dehydrogenase screen-printed biosensor based on enzyme and nanoporous gold co-catalytic strategy. Biosens. Bioelectron. 211, 578–584. doi:10.1016/j.bios.2022.114376
Chen, C. P., Xu, H., Zhan, Q., Zhang, Y., Wang, B., Chen, C., et al. (2023). Preparation of novel HKUST-1-glucose oxidase composites and their application in biosensing. Microchim. Acta 190 (1), 10. doi:10.1007/s00604-022-05563-4
Chen, Z., Chen, J., Ni, D., Xu, W., Zhang, W., and Mu, W. (2024). Microbial dextran-hydrolyzing enzyme: properties, structural features, and versatile applications. Food Chem. 437, 137951. doi:10.1016/j.foodchem.2023.137951
Chmayssem, A., Shalayel, I., Marinesco, S., and Zebda, A. (2023). Investigation of GOx stability in a chitosan matrix: applications for enzymatic electrodes. Sensors 23 (1), 465. doi:10.3390/s23010465
Chu, Z., Liu, Y., Xu, Y., Shi, L., Peng, J., and Jin, W. (2015). In-situ fabrication of well-distributed gold nanocubes on thiol graphene as a third-generation biosensor for ultrasensitive glucose detection. Electrochimica Acta 176, 162–171. doi:10.1016/j.electacta.2015.06.123
D Costa, E. J., Higgins, I. J., and Turner, A. P. F. (1986). Quinoprotein glucose dehydrogenase and its application in an amperometric glucose sensor. Biosensors 2 (2), 71–87. doi:10.1016/0265-928x(86)80011-6
Diaz-Rodriguez, A., and Davis, B. G. (2011). Chemical modification in the creation of novel biocatalysts. Curr. Opin. Chem. Biol. 15 (2), 211–219. doi:10.1016/j.cbpa.2010.12.002
Dong, X., Huang, A., He, L., Cai, C., and You, T. (2024). Recent advances in foodborne pathogen detection using photoelectrochemical biosensors: from photoactive material to sensing strategy. Front. Sustain. Food Syst. 8. doi:10.3389/fsufs.2024.1432555
Donini, C. A., Silva, M. K. L., Bronzato, G. R., Leão, A. L., and Cesarino, I. (2020). Evaluation of a biosensor based on reduced graphene oxide and glucose oxidase enzyme on the monitoring of second-generation ethanol production. J. Solid State Electrochem. 24 (8), 2011–2018. doi:10.1007/s10008-019-04471-7
Fornerod, M. J. J., Alvarez-Fernandez, A., Michalska, M., Papakonstantinou, I., and Guldin, S. (2023). Glucose oxidase loading in ordered porous aluminosilicates: exploring the potential of surface modification for electrochemical glucose sensing. Chem. Mater. 35 (18), 7577–7587. doi:10.1021/acs.chemmater.3c01202
Ge, L., Zhao, Y. s., Mo, T., Li, J. r., and Li, P. (2012). Immobilization of glucose oxidase in electrospun nanofibrous membranes for food preservation. Food control. 26 (1), 188–193. doi:10.1016/j.foodcont.2012.01.022
Giri, P., Pagar, A. D., Patil, M. D., and Yun, H. (2021). Chemical modification of enzymes to improve biocatalytic performance. Biotechnol. Adv. 53, 107868. doi:10.1016/j.biotechadv.2021.107868
Gough, D. A., Lucisano, J. Y., and Tse, P. H. S. (1985). Two-dimensional enzyme electrode sensor for glucose. Anal. Chem. 57 (12), 2351–2357. doi:10.1021/ac00289a042
Guo, Z., Wang, M., Barimah, A. O., Chen, Q., Li, H., Shi, J., El-Seedi, H. R., et al. (2021). Label-free surface enhanced Raman scattering spectroscopy for discrimination and detection of dominant apple spoilage fungus. Int. J. Food Microbiol., 338. doi:10.1016/j.ijfoodmicro.2020.108990
Halalipour, A., Duff, M. R., Howell, E. E., and Reyes-De-Corcuera, J. I. (2020). Catalytic activity and stabilization of phenyl-modified glucose oxidase at high hydrostatic pressure. Enzyme Microb. Technol. 137, 109538. doi:10.1016/j.enzmictec.2020.109538
Han, E., Li, L., Gao, T., Pan, Y., and Cai, J. (2024). Nitrite determination in food using electrochemical sensor based on self-assembled MWCNTs/AuNPs/poly-melamine nanocomposite. Food Chem. 437, 137773. doi:10.1016/j.foodchem.2023.137773
Han, J., Feng, H., Wu, J., Li, Y., Zhou, Y., Wang, L., et al. (2021). Construction of multienzyme Co-immobilized hybrid nanoflowers for an efficient conversion of cellulose into glucose in a cascade reaction. J. Agric. Food Chem. 69 (28), 7910–7921. doi:10.1021/acs.jafc.1c02056
He, P., Hassan, M. M., Tang, F., Jiang, H., Chen, M., Liu, R., et al. (2022). Total fungi counts and metabolic dynamics of volatile organic compounds in paddy contaminated by Aspergillus Niger during storage employing gas chromatography-ion mobility spectrometry. Food Anal. Methods 15 (6), 1638–1651. doi:10.1007/s12161-021-02186-y
He, W.-S., Zhu, H., and Chen, Z.-Y. (2018). Plant sterols: chemical and enzymatic structural modifications and effects on their cholesterol-lowering activity. J. Agric. Food Chem. 66 (12), 3047–3062. doi:10.1021/acs.jafc.8b00059
Hosseinkhani, S., Ranjbar, B., Naderi-Manesh, H., and Nemat-Gorgani, M. (2004). Chemical modification of glucose oxidase: possible formation of molten globule-like intermediate structure. FEBS Lett. 561 (1-3), 213–216. doi:10.1016/s0014-5793(04)00134-6
Hou, F., Ding, W., Qu, W., Oladejo, A. O., Xiong, F., Zhang, W., et al. (2017). Alkali solution extraction of rice residue protein isolates: influence of alkali concentration on protein functional, structural properties and lysinoalanine formation. Food Chem. 218, 207–215. doi:10.1016/j.foodchem.2016.09.064
Hovancová, J., Šišoláková, I., Oriňaková, R., and Oriňak, A. (2017). Nanomaterial-based electrochemical sensors for detection of glucose and insulin. J. Solid State Electrochem. 21 (8), 2147–2166. doi:10.1007/s10008-017-3544-0
Hu, T., Xu, K., Qiu, S., Han, Y., Chen, J., Xu, J., et al. (2020). Colorimetric detection of urine glucose using a C/CdTe QDs-GOx aerogel based on a microfluidic assay sensor. J. Mater. Chem. B 8 (32), 7160–7165. doi:10.1039/d0tb00328j
Hu, Y., Wang, X., Li, W., Lai, Y., Chen, Y., Wei, Z., et al. (2024). Metal-organic frameworks and related materials for nonenzymatic electrochemical glucose sensors. Int. J. Electrochem. Sci. 19 (2), 100466. doi:10.1016/j.ijoes.2024.100466
Huang, F., Wang, X., and Cao, Y. (2022). Mechanism and research progress of glucose oxidase in animal feed. Chin. J. Animal Nutr. 34 (9), 5500–5515. doi:10.3969/j.issn.1006-267x.2022.09.006
Hyun, K., Han, S. W., Koh, W. G., and Kwon, Y. (2015). Direct electrochemistry of glucose oxidase immobilized on carbon nanotube for improving glucose sensing. Int. J. Hydrogen Energy 40 (5), 2199–2206. doi:10.1016/j.ijhydene.2014.12.019
Jiang, C., Wang, X., Hou, B., Hao, C., Li, X., and Wu, J. (2020). Construction of a lignosulfonate-lysine hydrogel for the adsorption of heavy metal ions. J. Agric. Food Chem. 68 (10), 3050–3060. doi:10.1021/acs.jafc.9b07540
Jing, Y., Zhang, Y., Han, I., Wang, P., Mei, Q., and Huang, Y. (2020). Effects of different straw biochars on soil organic carbon, nitrogen, available phosphorus, and enzyme activity in paddy soil. Sci. Rep. 10 (1), 8837. doi:10.1038/s41598-020-65796-2
Kaçar, C., Dalkiran, B., Erden, P. E., and Kiliç, E. (2014). An amperometric hydrogen peroxide biosensor based on Co3O4 nanoparticles and multiwalled carbon nanotube modified glassy carbon electrode. Appl. Surf. Sci. 311, 139–146. doi:10.1016/j.apsusc.2014.05.028
Kajiwara, S., Komatsu, K., Yamada, R., Matsumoto, T., Yasuda, M., and Ogino, H. (2019). Improvement of the organic solvent stability of a commercial lipase by chemical modification with dextran. Biochem. Eng. J. 142, 1–6. doi:10.1016/j.bej.2018.11.003
Khan, G. F., Ohwa, M., and Wernet, W. (1996). Design of a stable charge transfer complex electrode for a third-generation amperometric glucose sensor. Anal. Chem. 68, 2939–2945. doi:10.1021/ac9510393
Khatami, S. H., Vakili, O., Ahmadi, N., Soltani Fard, E., Mousavi, P., Khalvati, B., et al. (2022). Glucose oxidase: applications, sources, and recombinant production. Biotechnol. Appl. Biochem. 69 (3), 939–950. doi:10.1002/bab.2165
Kilic, N. M., Singh, S., Keles, G., Cinti, S., Kurbanoglu, S., and Odaci, D. (2023). Novel approaches to enzyme-based electrochemical nanobiosensors. Biosensors-Basel 13 (6), 622. doi:10.3390/bios13060622
Kim, Y.-P., Park, S. J., Lee, D., and Kim, H. S. (2012). Electrochemical glucose biosensor by electrostatic binding of PQQ-glucose dehydrogenase onto self-assembled monolayers on gold. J. Appl. Electrochem. 42 (6), 383–390. doi:10.1007/s10800-012-0409-1
Kunugi, S., Murakami, Y., Ikeda, K., and Itoh, N. (1992). Chemical modification of proteins under high pressure. Preparation of ferrocene-attached bovine serum albumin and glucose oxidase. J. Biol. Chem. 14 (4), 210–214. doi:10.1016/s0141-8130(05)80029-7
Li, H., Xiao, N., Jiang, M., Long, J., Li, Z., and Zhu, Z. (2024a). Advances of transition metal-based electrochemical non-enzymatic glucose sensors for glucose analysis: a review. Crit. Rev. Anal. Chem., 1–37. doi:10.1080/10408347.2024.2339955
Li, T., Cheng, C., and Liu, J. (2024b). Chemical and enzyme-mediated chemical reactions for studying nucleic acids and their modifications. Chembiochem 25 (15), e202400220. doi:10.1002/cbic.202400220
Li, X., Li, C., Zhang, S., Cui, C., Li, J., and Gao, Q. (2021). Simple and fast colorimetric and electrochemical methods for the ultrasensitive detection of glucose. Anal. Bioanal. Chem. 413 (23), 5725–5731. doi:10.1007/s00216-021-03547-6
Liang, Z. Q., Yan, Y., Zhang, W., Luo, H., Yao, B., Huang, H., et al. (2023). Review of glucose oxidase as a feed additive: production, engineering, applications, growth-promoting mechanisms, and outlook. Crit. Rev. Biotechnol. 43 (5), 698–715. doi:10.1080/07388551.2022.2057275
Lin, M.-J., Wu, C.-C., and Chang, K.-S. (2019). Effect of poly-l-lysine polycation on the glucose oxidase/ferricyanide composite-based second-generation blood glucose sensors. Sensors 19 (6), 1448. doi:10.3390/s19061448
Liu, G., Liu, Z., Sun, Y., Sun, M., Duan, J., Tian, Y., et al. (2024). Cascade amplifying electrochemical bioanalysis for zearalenone detection in agricultural products: utilizing a glucose-fenton-HQ system on bimetallic-ZIF@CNP nanocomposites. Foods 13 (19), 3192. doi:10.3390/foods13193192
Liu, J., Kong, N., Li, A., Luo, X., Cui, L., Wang, R., et al. (2013). Graphene bridged enzyme electrodes for glucose biosensing application. Analyst 138 (9), 2567–2575. doi:10.1039/c3an36929c
Liu, J. M., Shen, X., Baimanov, D., Wang, L., Xiao, Y., Liu, H., et al. (2019). Immobilized ferrous ion and glucose oxidase on graphdiyne and its application on one-step glucose detection. Acs Appl. Mater. and Interfaces 11 (3), 2647–2654. doi:10.1021/acsami.8b03118
Lv, C., Yang, X., Wang, Z., Ying, M., Han, Q., and Li, S. (2021). Enhanced performance of bioelectrodes made with amination-modified glucose oxidase immobilized on carboxyl-functionalized ordered mesoporous carbon. Nanomaterials 11 (11), 3086. doi:10.3390/nano11113086
Ma, S., Pan, L. g., You, T., and Wang, K. (2021). g-C3N4/Fe3O4 nanocomposites as adsorbents analyzed by UPLC-MS/MS for highly sensitive simultaneous determination of 27 mycotoxins in maize: aiming at increasing purification efficiency and reducing time. J. Agric. Food Chem. 69 (16), 4874–4882. doi:10.1021/acs.jafc.1c00141
Ma, S., Wang, M., You, T., and Wang, K. (2019). Using magnetic multiwalled carbon nanotubes as modified QuEChERS adsorbent for simultaneous determination of multiple mycotoxins in grains by UPLC-MS/MS. J. Agric. Food Chem. 67 (28), 8035–8044. doi:10.1021/acs.jafc.9b00090
Ma, X., Huang, W., Song, Y., Han, J., Wu, J., Wang, L., et al. (2022). Novel recyclable UCST-type immobilized glucose isomerase biocatalyst with excellent performance for isomerization of glucose to fructose. J. Agric. Food Chem. 70 (43), 13959–13968. doi:10.1021/acs.jafc.2c05667
Malitesta, C., Palmisano, F., Torsi, L., and Zambonin, P. G. (1990). Glucose fast-response amperometric sensor based on glucose oxidase immobilized in an electropolymerized poly(o-phenylenediamine) film. Anal. Chem. 62 (24), 2735–2740. doi:10.1021/ac00223a016
Mao, M. F., Limin, L., Chuanhui, H., Zhenyu, L., and Weijia, W. (2025). Highly sensitive electrochemiluminescence glucose sensor under alkaline conditions based on glucose oxidase@conductive metal-organic framework nanocapsules. Sensors Actuators B-Chemical, 434. doi:10.1016/j.snb.2025.137595
Min, S. Y., Yu, Q., Ye, J., Hao, P., Ning, J., Hu, Z., et al. (2023). Nanomaterials with glucose oxidase-mimicking activity for biomedical applications. Molecules 28 (12), 4615. doi:10.3390/molecules28124615
Ming, T., Lan, T., Yu, M., Duan, X., Cheng, S., Wang, H., et al. (2024). A novel electrochemical microneedle sensor for highly sensitive real time monitoring of glucose. Microchem. J., 207. doi:10.1016/j.microc.2024.112021
Monkrathok, J., Janphuang, P., Suphachiaraphan, S., Kampaengsri, S., Kamkaew, A., Chansaenpak, K., et al. (2024). Enhancing glucose biosensing with graphene oxide and ferrocene-modified linear poly(ethylenimine). Biosensors 14 (4), 161. doi:10.3390/bios14040161
Morales-Narvaez, E., Baptista-Pires, L., Zamora-Gálvez, A., and Merkoçi, A. (2017). Graphene-based biosensors: going simple. Adv. Mater 29 (7). doi:10.1002/adma.201604905
Mtemeri, L., and Hickey, D. P. (2023). Model-driven design of redox mediators: quantifying the impact of quinone structure on bioelectrocatalytic activity with glucose oxidase. J. Phys. Chem. B 127 (36), 7685–7693. doi:10.1021/acs.jpcb.3c03740
Naikoo, G. A., Awan, T., Salim, H., Arshad, F., Hassan, I. U., Pedram, M. Z., et al. (2022). Fourth-generation glucose sensors composed of copper nanostructures for diabetes management: a critical review. Bioeng. and Transl. Med. 7 (1), e10248. doi:10.1002/btm2.10248
Nikitina, V. N., Karastsialiova, A. R., and Karyakin, A. A. (2023). Glucose test strips with the largest linear range made via single step modification by glucose oxidase-hexacyanoferrate-chitosan mixture. Biosens. Bioelectron. 220, 114851. doi:10.1016/j.bios.2022.114851
Noro, J., Cavaco-Paulo, A., and Silva, C. (2022). Chemical modification of lipases: a powerful tool for activity improvement. Biotechnol. J. 17 (8), e2100523. doi:10.1002/biot.202100523
Ou, L., Liu, G., and Xia, N. (2021). Research progress and application prospects of electrochemical glucose sensors. Int. J. Electrochem. Sci. 16 (6), 210633. doi:10.20964/2021.06.42
Pakapongpan, S., and Poo-Arporn, R. P. (2017). Self-assembly of glucose oxidase on reduced graphene oxide-magnetic nanoparticles nanocomposite-based direct electrochemistry for reagentless glucose biosensor. Mater Sci. Eng. C Mater Biol. Appl. 76, 398–405. doi:10.1016/j.msec.2017.03.031
Peng, Y., Sun, Q., and Park, Y. (2019). Chicoric acid promotes glucose uptake and Akt phosphorylation via AMP-activated protein kinase α-dependent pathway. J. Funct. Foods 59, 8–15. doi:10.1016/j.jff.2019.05.020
Pickering, G. J., Heatherbell, D. A., and Barnes, M. F. (1998). Optimising glucose conversion in the production of reduced alcohol wine using glucose oxidase. Food Res. Int. 31 (10), 685–692. doi:10.1016/s0963-9969(99)00046-0
Pohanka, M. (2021). Glucose electrochemical biosensors: the past and current trends. Int. J. Electrochem. Sci. 16 (7), 210719. doi:10.20964/2021.07.52
Prajapati, S. K., Malaiya, A., Kesharwani, P., Soni, D., and Jain, A. (2022). Biomedical applications and toxicities of carbon nanotubes. Drug Chem. Toxicol. 45 (1), 435–450. doi:10.1080/01480545.2019.1709492
Qin, C., Guo, W., Liu, Y., Liu, Z., Qiu, J., and Peng, J. (2017). A novel electrochemical sensor based on graphene oxide decorated with silver nanoparticles–molecular imprinted polymers for determination of sunset yellow in soft drinks. Food Anal. Methods 10 (7), 2293–2301. doi:10.1007/s12161-016-0753-6
Rafighi, P., Tavahodi, M., and Haghighi, B. (2016). Fabrication of a third-generation glucose biosensor using graphene-polyethyleneimine-gold nanoparticles hybrid. Sensors Actuators B Chem. 232, 454–461. doi:10.1016/j.snb.2016.03.147
Ramachandran, T., Mourad, A. I., Raji, R. K., Krishnapriya, R., Cherupurakal, N., Subhan, A., et al. (2022). KOH mediated hydrothermally synthesized hexagonal-CoMn2O4 for energy storage supercapacitor applications. Int. J. Energy Res. 46 (12), 16823–16838. doi:10.1002/er.8350
Ravikumar, Y., Ponpandian, L. N., Zhang, G., Yun, J., and Qi, X. (2021). Harnessing -arabinose isomerase for biological production of -tagatose: recent advances and its applications. Trends Food Sci. and Technol. 107, 16–30. doi:10.1016/j.tifs.2020.11.020
Ren, X., Liang, Q., Zhang, X., Hou, T., Li, S., and Ma, H. (2018). Stability and antioxidant activities of corn protein hydrolysates under simulated gastrointestinal digestion. Cereal Chem. 95 (6), 760–769. doi:10.1002/cche.10092
Rong, J., Zhou, Z., Wang, Y., Han, J., Li, C., Zhang, W., et al. (2019). Immobilization of horseradish peroxidase on multi-armed magnetic graphene oxide composite: improvement of loading amount and catalytic activity. Food Technol. Biotechnol. 57 (2), 260–271. doi:10.17113/ftb.57.02.19.5832
Shah, S. S., Niaz, F., Ehsan, M. A., Das, H. T., Younas, M., Khan, A. S., et al. (2024). Advanced strategies in electrode engineering and nanomaterial modifications for supercapacitor performance enhancement: a comprehensive review. J. Energy Storage 79, 110152. doi:10.1016/j.est.2023.110152
Shang, L., Bai, X., Chen, C., Liu, L., Li, M., Xia, X., et al. (2019). Isolation and identification of a Bacillus megaterium strain with ochratoxin A removal ability and antifungal activity. Food control. 106, 106743. doi:10.1016/j.foodcont.2019.106743
Shen, L., Zhou, X., Zhang, C., Yin, H., Wang, A., and Wang, C. (2019). Functional characterization of bimetallic CuPdxnanoparticles in hydrothermal conversion of glycerol to lactic acid. J. Food Biochem. 43 (8), e12931. doi:10.1111/jfbc.12931
Shi, T., Yuan, L., Mu, J., and Gao, R. (2019). The effect of Arginine, Lysine and Histidine in the myosin secondary structure by circular dichroism and Raman spectroscopy. Cyta-Journal Food 17 (1), 656–660. doi:10.1080/19476337.2019.1635648
Song, C., Guo, J., Wang, Y., Xiang, H., and Yang, Y. (2024). Electrochemical glucose sensors: classification, catalyst innovation, and sampling mode evolution. Biotechnol. J. 19 (10), e202400349. doi:10.1002/biot.202400349
Soni, S. K., Thomas, B., and Kar, V. R. (2020). A comprehensive review on CNTs and CNT-reinforced composites: syntheses, characteristics and applications. Mater. Today Commun. 25, 101546. doi:10.1016/j.mtcomm.2020.101546
Sridharan, R., Monisha, B., Kumar, P. S., and Gayathri, K. V. (2022). Carbon nanomaterials and its applications in pharmaceuticals: a brief review. Chemosphere 294, 133731. doi:10.1016/j.chemosphere.2022.133731
Su, X., et al. (2023). An advanced ratiometric molecularly imprinted sensor based on metal ion reoxidation for indirect and ultrasensitive glyphosate detection in fruit. Food Chem., 429. doi:10.1016/j.foodchem.2023.136927
Sumio, I. a. (1991). Helical microtubules of graphitic carbon. Nature 354 (6348), 56–58. doi:10.1038/354056a0
Sun, B., and Joshi, R. (2010). Formulation of 2D graphene deformation based on chiral-tube base vectors. J. Nanomater. 2010 (1). doi:10.1155/2010/402591
Sun, J., Zhou, X., Mao, H., Wu, X., Zhang, X., Gao, H., et al. (2016). Identification of pesticide residue level in lettuce based on hyperspectra and chlorophyll fluorescence spectra. Int. J. Agric. Biol. Eng. 9 (6), 231–239. doi:10.3965/j.ijabe.20160906.2519
Tatsumi, H., and Osaku, N. (2011). Sensitive electrochemical detection of the hydroxyl radical using enzyme-catalyzed redox cycling. Anal. Sci. 27 (11), 1065–1067. doi:10.2116/analsci.27.1065
Teymourian, H., Barfidokht, A., and Wang, J. (2020). Electrochemical glucose sensors in diabetes management: an updated review (2010–2020). Chem. Soc. Rev. 49 (21), 7671–7709. doi:10.1039/d0cs00304b
Thirumalraj, B., Rajkumar, C., Chen, S. M., Veerakumar, P., Perumal, P., and Liu, S. B. (2018). Carbon aerogel supported palladium-ruthenium nanoparticles for electrochemical sensing and catalytic reduction of food dye. Sensors Actuators B-Chemical 257, 48–59. doi:10.1016/j.snb.2017.10.112
Tong, X. L., Jiang, L., Ao, Q., Lv, X., Song, Y., and Tang, J. (2024). “Highly stable glucose oxidase polynanogel@MXene/chitosan electrochemical biosensor based on a multi-stable interface structure for glucose detection,”, 248. Biosens. and Bioelectron. doi:10.1016/j.bios.2023.115942
Tzanov, T., Costa, S. A., Gübitz, G. M., and Cavaco-Paulo, A. (2002). Hydrogen peroxide generation with immobilized glucose oxidase for textile bleaching. J. Biotechnol. 93, 87–94. doi:10.1016/s0168-1656(01)00386-8
Upadhyay, A. K., Chen, S. M., Ting, T. W., and Peng, Y. Y. (2011). A biosensor for hydrogen peroxide based on single walled carbon nanotube and metal oxide modified indium tin oxide electrode. Int. J. Electrochem. Sci. 6 (8), 3466–3482. doi:10.1016/s1452-3981(23)18265-4
Vaidyanathan, V. K., Alanazi, A. K., Kumar, P. S., Rajendran, D. S., Chidambaram, A., Venkataraman, S., et al. (2023). Cost-effective, scalable production of glucose oxidase using Casuarina equisetifolia biomass and its application in the bio-Fenton oxidation process for the removal of trace organic contaminants from wastewater. Bioresour. Technol., 377. doi:10.1016/j.biortech.2023.128958
Wang, F., Chen, X., Wang, Y., Li, X., Wan, M., Zhang, G., et al. (2022). Insights into the structures, inhibitors, and improvement strategies of glucose oxidase. Int. J. Mol. Sci. 23 (17), 9841. doi:10.3390/ijms23179841
Wang, F., Zhu, Y., Qian, L., Yin, Y., Yuan, Z., Dai, Y., et al. (2024). Lamellar Ti 3 C 2 MXene composite decorated with platinum-doped MoS 2 nanosheets as electrochemical sensing functional platform for highly sensitive analysis of organophosphorus pesticides. Food Chem. 459, 140379. doi:10.1016/j.foodchem.2024.140379
Wang, J. a.F. L., and Lu, F. (2009). Oxygen-rich oxidase enzyme electrodes for operation in oxygen-free solutions. J. Am. Chem. Soc. 120 (5), 1048–1050. doi:10.1021/ja972759p
Wang, L., Li, W., Liu, Y., Zhi, W., Han, J., Wang, Y., et al. (2019b). Green separation of bromelain in food sample with high retention of enzyme activity using recyclable aqueous two-phase system containing a new synthesized thermo-responsive copolymer and salt. Food Chem. 282, 48–57. doi:10.1016/j.foodchem.2019.01.005
Wang, P., Hassan, M. M., Guo, Z., Zhang, Z. Z., and Chen, Q. (2019a). Fabricating an acetylcholinesterase modulated UCNPs-Cu2+fluorescence Biosensor for ultrasensitive Detection of organophosphorus pesticides-Diazinon in food. J. Agric. Food Chem. 67 (14), 4071–4079. doi:10.1021/acs.jafc.8b07201
Wang, X., Li, Q., Xie, J., Jin, Z., Wang, J., Li, Y., et al. (2009). Fabrication of ultralong and electrically uniform single-walled carbon nanotubes on clean substrates. Nano Lett. 9 (9), 3137–3141. doi:10.1021/nl901260b
Wang, X., Xu, Y., Li, Y., Li, Y., Li, Z., Zhang, W., et al. (2021). Rapid detection of cadmium ions in meat by a multi-walled carbon nanotubes enhanced metal-organic framework modified electrochemical sensor. Food Chem. 357, 129762. doi:10.1016/j.foodchem.2021.129762
Wen, C., Zhang, J., Feng, Y., Duan, Y., Ma, H., and Zhang, H. (2020). Purification and identification of novel antioxidant peptides from watermelon seed protein hydrolysates and their cytoprotective effects on H2O2-induced oxidative stress. Food Chem. 327, 127059. doi:10.1016/j.foodchem.2020.127059
Wijayanti, S. D., Schachinger, F., Ludwig, R., and Haltrich, D. (2023). Electrochemical and biosensing properties of an FAD-dependent glucose dehydrogenase from Trichoderma virens. Bioelectrochemistry 153, 108480. doi:10.1016/j.bioelechem.2023.108480
Wu, B., Xu, H., Shi, Y., Yao, Z., Yu, J., Zhou, H., et al. (2022). Microelectrode glucose biosensor based on nanoporous platinum/graphene oxide nanostructure for rapid glucose detection of tomato and cucumber fruits. Food Qual. Saf. 6. doi:10.1093/fqsafe/fyab030
Wu, S., Duan, N., Qiu, Y., Li, J., and Wang, Z. (2017). Colorimetric aptasensor for the detection of Salmonella enterica serovar typhimurium using ZnFe2O4-reduced graphene oxide nanostructures as an effective peroxidase mimetics. Int. J. Food Microbiol. 261, 42–48. doi:10.1016/j.ijfoodmicro.2017.09.002
Xia, J., Feng, L., Lishi, Y., Hongbo, S., Jingya, Q., and Bin, Z. (2023). Simultaneous determination of tert-butylhydroquinone, butylated hydroxyanisole and phenol in plant oil by metalloporphyrin-based covalent organic framework electrochemical sensor. J. Food Compos. Analysis 122, 105486. doi:10.1016/j.jfca.2023.105486
Xia, J., Zou, B., Liu, F., Wang, P., and Yan, Y. (2022). Sensitive glucose biosensor based on cyclodextrin modified carbon nanotubes for detecting glucose in honey. J. Food Compos. Analysis 105, 104221. doi:10.1016/j.jfca.2021.104221
Xu, Y., Zhang, W., Shi, J., Zou, X., Li, Y., Haroon Elrasheid, T., et al. (2017). Electrodeposition of gold nanoparticles and reduced graphene oxide on an electrode for fast and sensitive determination of methylmercury in fish. Food Chem. 237, 423–430. doi:10.1016/j.foodchem.2017.05.096
Xu, Y., Zhang, W., Shi, J., Zou, X., Li, Z., and Zhu, Y. (2016). Microfabricated interdigitated Au electrode for voltammetric determination of lead and cadmium in Chinese mitten crab (Eriocheir sinensis). Food Chem. 201, 190–196. doi:10.1016/j.foodchem.2016.01.078
Yan, M., Chen, Y., Feng, Y., Saeed, M., Fang, Z., Zhen, W., et al. (2024). Perspective on agricultural industrialization: modification strategies for enhancing the catalytic capacity of keratinase. J. Agric. Food Chem. 72 (23), 13537–13551. doi:10.1021/acs.jafc.4c03025
Yin, L., Jayan, H., Cai, J., El-Seedi, H. R., Guo, Z., and Zou, X. (2023). Development of a sensitive SERS method for label-free detection of hexavalent chromium in tea using carbimazole redox reaction. Foods 12 (14), 2673. doi:10.3390/foods12142673
Yin, S. J., et al. (2024). A long lifetime and highly sensitive wearable microneedle sensor for the continuous real-time monitoring of glucose in interstitial fluid. Amsterdam, Netherlands Biosensors and Bioelectronics, 244.
Yu, H. B., Feng, J., Zhong, F., and Wu, Y. (2022). Chemical modification for the “Off-/On” regulation of enzyme activity. Macromol. Rapid Commun. 43 (18), e2200195. doi:10.1002/marc.202200195
Yu, Z. H., Kou, Y., Dai, Y., Wang, X., Wei, H., and Xia, D. (2015). Direct electrochemistry of glucose oxidase on a three-dimensional porous zirconium phosphate-carbon aerogel composite. Electrocatalysis 6 (4), 341–347. doi:10.1007/s12678-015-0249-y
Zappelli, P., Pappa, R., Rossodivita, A., and Re, L. (1978). Carboxylic and polyethyleneimine-bound FAD derivatives. Synthesis, coenzymic properties, conformational and enzyme-coenzyme interaction studies. IEEE 89 (2), 491–499. doi:10.1111/j.1432-1033.1978.tb12553.x
Zeng, K., Chen, B., Li, Y., Meng, H., Wu, Q., Yang, J., et al. (2022). Gold nanoparticle-carbon nanotube nanohybrids with peroxidase-like activity for the highly-sensitive immunoassay of kanamycin in milk. Int. J. Food Sci. Technol. 57 (9), 6028–6037. doi:10.1111/ijfs.15955
Zeng, K., Wei, W., Jiang, L., Zhu, F., and Du, D. (2016). Use of carbon nanotubes as a solid support to establish quantitative (centrifugation) and qualitative (filtration) immunoassays to detect gentamicin contamination in commercial milk. J. Agric. Food Chem. 64 (41), 7874–7881. doi:10.1021/acs.jafc.6b03332
Zhang, C., Shi, X., Yu, F., and Quan, Y. (2020). Preparation of dummy molecularly imprinted polymers based on dextran-modified magnetic nanoparticles Fe3O4 for the selective detection of acrylamide in potato chips. Food Chem. 317, 126431. doi:10.1016/j.foodchem.2020.126431
Zhang, D., Wang, Y., Sun, X., Liu, Y., Zhou, Y., Shin, H., et al. (2019). Voltammetric, spectroscopic, and cellular characterization of redox functionality of eckol and phlorofucofuroeckol-A: a comparative study. J. Food Biochem. 43 (7), e12845. doi:10.1111/jfbc.12845
Zhang, L., Chen, Q., Han, X., and Zhang, Q. (2018b). MnO2Nanoparticles and carbon nanofibers Nanocomposites with high sensing performance toward glucose. J. Clust. Sci. 29 (6), 1089–1098. doi:10.1007/s10876-018-1421-3
Zhang, Y., Cui, Y., Hong, X., and Du, D. (2018a). Using of tyramine signal amplification to improve the sensitivity of ELISA for aflatoxin B1 in edible oil samples. Food Anal. Methods 11 (9), 2553–2560. doi:10.1007/s12161-018-1235-9
Zhang, Y., Hu, Y., Wilson, G. S., Moatti-Sirat, D., Poitout, V., and Reach, G. (1994). Elimination of the acetaminophen interference in an implantable glucose sensor. Anal. Chem. 66 (7), 1183–1188. doi:10.1021/ac00079a038
Zhao, S., Guo, D., Zhu, Q., Dou, W., and Guan, W. (2021). Display of microbial glucose dehydrogenase and cholesterol oxidase on the yeast cell surface for the detection of blood biochemical parameters. Biosensors-Basel 11 (1), 13. doi:10.3390/bios11010013
Zhao, W., Xu, J. J., Qiu, Q. Q., and Chen, H. Y. (2006). Nanocrystalline diamond modified gold electrode for glucose biosensing. Biosens. and Bioelectron. 22 (5), 649–655. doi:10.1016/j.bios.2006.01.026
Zhao, Y., Ma, Y., Zhou, R., He, Y., Wu, Y., Yi, Y., et al. (2022). Highly sensitive electrochemical detection of paraoxon ethyl in water and fruit samples based on defect-engineered graphene nanoribbons modified electrode. J. Food Meas. Charact. 16 (4), 2596–2603. doi:10.1007/s11694-022-01366-6
Zhou, G., Cao, Z., Liu, Y., Zheng, H., and Xu, K. (2024). Highly sensitive and stable glucose sensing using N-type conducting polymer based organic electrochemical transistor. J. Electroanal. Chem. 952, 117961. doi:10.1016/j.jelechem.2023.117961
Zhou, W., Li, C., Sun, C., and Yang, X. (2016). Simultaneously determination of trace Cd2+ and Pb2+ based on L-cysteine/graphene modified glassy carbon electrode. Food Chem. 192, 351–357. doi:10.1016/j.foodchem.2015.07.042
Zhu, C., Du, D., and Lin, Y. (2017). Graphene-like 2D nanomaterial-based biointerfaces for biosensing applications. Biosens. Bioelectron. 89 (Pt 1), 43–55. doi:10.1016/j.bios.2016.06.045
Keywords: glucose oxidase, electrochemical sensing, carbon nanomaterials, glucose detection, chemical modification
Citation: Guoqiang G, Liang Q, Yani Z, Pengyun W, Fanzhuo K, Yuyang Z, Zhiyuan L, Xing N, Xue Z, Qiongya L and Bin Z (2025) Recent advances in glucose monitoring utilizing oxidase electrochemical biosensors integrating carbon-based nanomaterials and smart enzyme design. Front. Chem. 13:1591302. doi: 10.3389/fchem.2025.1591302
Received: 12 March 2025; Accepted: 09 April 2025;
Published: 28 April 2025.
Edited by:
Shuai Wang, Qilu University of Technology, ChinaReviewed by:
Yi Hu, Hong Kong Polytechnic University, Hong Kong SAR, ChinaTholkappiyan Ramachandran, Khalifa University, United Arab Emirates
Copyright © 2025 Guoqiang, Liang, Yani, Pengyun, Fanzhuo, Yuyang, Zhiyuan, Xing, Xue, Qiongya and Bin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Zou Bin, Ymluem91MjAwOUB1anMuZWR1LmNu