ORIGINAL RESEARCH article
Front. Chem.
Sec. Chemical Biology
Volume 13 - 2025 | doi: 10.3389/fchem.2025.1627286
Molecular functional mechanisms of two alcohol acetyltransferases in Lavandula x intermedia (lavandin)
Provisionally accepted
Dafeng Liu1*
Yanyan Du1Ablikim Abdiriyim1Lvxia Zhang1Daoqi Song2Huashui Deng2Xiongying Wen1Yanyan Zhang1Bingwang Sun1- 1Yili Normal University, Yili, China
- 2Xiamen University, Xiamen, China
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Volatile esters are key flavor components in most plants, including Lavandula x intermedia (lavandin). The final step in ester biosynthesis is catalyzed by Lavandula x intermedia alcohol acyltransferases (LiAATases), which attach alcohols to acyl groups.However, the functional role and mechanism of LiAATases remain poorly understood.Here, we predicted their structural models using AlphaFold2 and identified potential active site residues through the GalaxyWEB program. Catalytic assays were optimized at pH 7.5 and 30 o C. Substrate specificity for alcohols was assessed for both enzymes.Gene expression analysis revealed that LiAATase1 and LiAATase2 were most highly expressed in the petals and pistils, respectively, with peak expression occurring at stage 4 for LiAATase1 and stage 1 for LiAATase2. Our study aims to elucidate the functional properties of alcohol acyltransferases in Lavandula x intermedia, contributing to an understanding of ester biosynthesis and specificity in this species.
Keywords: Lavandula x intermedia (Lavandin), Alcohol acetyltransferase, structural prediction, enzyme activity assay, Gene Expression Levels
Received: 12 May 2025; Accepted: 27 May 2025.
Copyright: © 2025 Liu, Du, Abdiriyim, Zhang, Song, Deng, Wen, Zhang and Sun. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Dafeng Liu, Yili Normal University, Yili, China
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