ORIGINAL RESEARCH article
Front. Chem.
Sec. Analytical Chemistry
Volume 13 - 2025 | doi: 10.3389/fchem.2025.1632033
This article is part of the Research TopicFrontiers in Chemistry: Rising Stars in ChinaView all 3 articles
UPLC Fingerprinting Combined with Quantitative Analysis of Multicomponents by A Single Marker (QAMS) for Quality Evaluation of YiQing Granules
Provisionally accepted
- 1Chongqing Medical University, Chongqing, China
- 2Chongqing University of Chinese Medicine, Chongqing, China
- 3Chongqing Institute of Pharmaceutical Plant, Chongqing, China
- 4Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan Province, China
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Research background: YiQing granules (YQGs) are a patented drug commonly used in clinics in China. However, the quality control index of this preparation is relatively limited, which does not effectively ensure the quality of the product. Thus, a comprehensive quality evaluation of YQGs is lacking.Objective: The aim of this study was to establish a method for quantitative and qualitative analysis of YQGs based on ultra-high performance liquid chromatography-photodiode array detector (UPLC-PAD) fingerprint combined with quantitative analysis of multicomponents by a single marker (QAMS).Methods: We established and verified the comprehensive evaluation method that UPLC fingerprint combined with QAMS and stoichiometric method, to assess the overall quality of YQGs produced by different manufacturers. Berberine was selected as an internal reference, and the relative correction factors of coptisine, epiberberine, baicalin, berberine, palmatine, wogonoside, baicalein, wogonin, aloe-emodin, rhein, emodin, and chrysophanol were established.Results showed that the experimental time of the fingerprint was significantly reduced to approximately 0.5 h using the UPLC-PAD method. A total of 32 common peaks with similarity greater than 0.9 were identified. The accuracy of QAMS was compared with the external standard method, with no significant difference between the two methods.The UPLC fingerprint combined with QAMS method established in this study is feasible and reliable; it can be used for the comprehensive quality evaluation of YQGs and can provide a reference for the quality evaluation of other traditional Chinese medicine preparations.
Keywords: "YiQing" granule, quantitative analysis of multicomponents by a single marker (QAMS), UPLC, Fingerprint, quantitative analysis, Quality control
Received: 20 May 2025; Accepted: 17 Jul 2025.
Copyright: © 2025 Li, Yu, Zhao, Pu, Zhong, Tao, Wang, Zhang and Cao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Dan Zhang, Chongqing Medical University, Chongqing, 400016, China
Weiguo Cao, Chongqing University of Chinese Medicine, Chongqing, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.